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The map label for this gene is hsrA [H]

Identifier: 148358984

GI number: 148358984

Start: 1667399

End: 1668766

Strand: Reverse

Name: hsrA [H]

Synonym: LPC_0869

Alternate gene names: 148358984

Gene position: 1668766-1667399 (Counterclockwise)

Preceding gene: 148358985

Following gene: 148358980

Centisome position: 46.66

GC content: 36.77

Gene sequence:

>1368_bases
ATGTTCATTGTATCATGCGCCATGTTTATGGAGGCTATTGATACAACTATTCTCAACACGGCAATCCCGGTGATGGCACA
TAGTTTAGAAGTCAATCCAATTGATCTCAAACTCGTGCTCATTAGCTATCTTTTAAGTTTGGCAATCTTTATTCCCATAA
GTGGATGGATTGCTGATAAATATGGTGTAAAAAAAGTATTTATAGCAGCTATTGGTGTCTTTACTCTAAGTTCCTTATGG
TGCGGATTCACTCAAAGTCTTTGGGAATTGGTATTAGCCAGAATTATACAGGGTTTAGGCGGCTCACTGACCGTGCCTGT
TGGACGTTTAATTATACTGAGAACTTGTAAGCGTCACGAATTAATAACAAAAATGAGTATTGTCGTCATGGTTGCATCGC
TAGGTATGCTACTTGGCCCCTTACTGGGCGGTATCATTACTGATTATTTCTCATGGCGGTGGATATTTTGGGTTAATATC
CCTGTTGGCATAATCGCCATGCTTTTATCTCACAAACTGCTTCCAACTATCCTGCCCAGATCGGTTCCACCTTTGGATAA
ATCAGGATTTATTATGTTCGGAGCAGGCCTTGCATTCTTGACTTTAGGTTTGTCTTTGTTTAGTGAGACGAAAACGGATA
TTGTCTACTCCGCTTCAATTACTCTTTTATCCGCCTTGCTATTAATTGGATATACCAAACATTCTCGTAACAAAAAAAAT
CCTATAATAAAAGTTACTTTGTTAAATATAAGAACATTTCGTATTGCCGTACTTGGAAATTTATTAACTCGTTTAAGTTT
TGGTGGAATTCCCTTTTTATTACCTTTACTCTTCCAAATTATTTTAGAATATTCTCCCCAGTTATCAGGAATGTTACTTA
CACCAGTAGCATTAGGCGTATTCCTGGTTAAACCATTATCTTTCTCCATCTTACGAAGGTTGGGTTATAAAAAATTACTC
CTTTTAAATACCGTTTTAGTCTGTTTTTCTCTCTGGTCCTTTGCCACAATAAATCAAAGCACTTCTGTCATCTACATTGG
TTTTTTAACATTTCTTTATGGATTTTTCATTGCTCTGCAATACACAGGGATGAATTCCCTTGCGTATGCGAATATTGACG
ATAATGATATGAGTTATGCAACAAGCATTATGAGCACAGTTCAACAATTATCCCAAAGTTTTGGAGTAGCAATTTCTGCC
CTACTGGTCAGTTTATTCACTTCTCAGGTTTCGCAGCATTTTGTTTTAACAGTCAAGATTTTTCATCTAACCTTTTTTGC
TTTAGGGATGCTTACGATTCTTTCAGGATTAATATTTACCAGCTTAAAAAAAGAGGATGGAAAAGAACTGATTGAGTCAC
CCACCTAG

Upstream 100 bases:

>100_bases
AAATACTGGAGCGGGCTTGTGACTTTAATAGAGTTTCAGGTTAATAAAATTTAAACGTTCTAACTTCCATAAAACAGGCA
TTATGAAGAAAAATATTATT

Downstream 100 bases:

>100_bases
ATTCGCATCAGTTGCATCACTAAGCAGACAGCAGGACGACCAAGACAATGCATATCGATGCAGTATCTGGTCATTCAAGA
AAAATTAATGCGTAAGTAAA

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MFIVSCAMFMEAIDTTILNTAIPVMAHSLEVNPIDLKLVLISYLLSLAIFIPISGWIADKYGVKKVFIAAIGVFTLSSLW
CGFTQSLWELVLARIIQGLGGSLTVPVGRLIILRTCKRHELITKMSIVVMVASLGMLLGPLLGGIITDYFSWRWIFWVNI
PVGIIAMLLSHKLLPTILPRSVPPLDKSGFIMFGAGLAFLTLGLSLFSETKTDIVYSASITLLSALLLIGYTKHSRNKKN
PIIKVTLLNIRTFRIAVLGNLLTRLSFGGIPFLLPLLFQIILEYSPQLSGMLLTPVALGVFLVKPLSFSILRRLGYKKLL
LLNTVLVCFSLWSFATINQSTSVIYIGFLTFLYGFFIALQYTGMNSLAYANIDDNDMSYATSIMSTVQQLSQSFGVAISA
LLVSLFTSQVSQHFVLTVKIFHLTFFALGMLTILSGLIFTSLKKEDGKELIESPT

Sequences:

>Translated_455_residues
MFIVSCAMFMEAIDTTILNTAIPVMAHSLEVNPIDLKLVLISYLLSLAIFIPISGWIADKYGVKKVFIAAIGVFTLSSLW
CGFTQSLWELVLARIIQGLGGSLTVPVGRLIILRTCKRHELITKMSIVVMVASLGMLLGPLLGGIITDYFSWRWIFWVNI
PVGIIAMLLSHKLLPTILPRSVPPLDKSGFIMFGAGLAFLTLGLSLFSETKTDIVYSASITLLSALLLIGYTKHSRNKKN
PIIKVTLLNIRTFRIAVLGNLLTRLSFGGIPFLLPLLFQIILEYSPQLSGMLLTPVALGVFLVKPLSFSILRRLGYKKLL
LLNTVLVCFSLWSFATINQSTSVIYIGFLTFLYGFFIALQYTGMNSLAYANIDDNDMSYATSIMSTVQQLSQSFGVAISA
LLVSLFTSQVSQHFVLTVKIFHLTFFALGMLTILSGLIFTSLKKEDGKELIESPT
>Mature_455_residues
MFIVSCAMFMEAIDTTILNTAIPVMAHSLEVNPIDLKLVLISYLLSLAIFIPISGWIADKYGVKKVFIAAIGVFTLSSLW
CGFTQSLWELVLARIIQGLGGSLTVPVGRLIILRTCKRHELITKMSIVVMVASLGMLLGPLLGGIITDYFSWRWIFWVNI
PVGIIAMLLSHKLLPTILPRSVPPLDKSGFIMFGAGLAFLTLGLSLFSETKTDIVYSASITLLSALLLIGYTKHSRNKKN
PIIKVTLLNIRTFRIAVLGNLLTRLSFGGIPFLLPLLFQIILEYSPQLSGMLLTPVALGVFLVKPLSFSILRRLGYKKLL
LLNTVLVCFSLWSFATINQSTSVIYIGFLTFLYGFFIALQYTGMNSLAYANIDDNDMSYATSIMSTVQQLSQSFGVAISA
LLVSLFTSQVSQHFVLTVKIFHLTFFALGMLTILSGLIFTSLKKEDGKELIESPT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=451, Percent_Identity=37.2505543237251, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI1788392, Length=453, Percent_Identity=35.5408388520971, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1789042, Length=452, Percent_Identity=25.8849557522124, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788710, Length=434, Percent_Identity=26.9585253456221, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI87081983, Length=398, Percent_Identity=26.6331658291457, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1790146, Length=166, Percent_Identity=25.9036144578313, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=139, Percent_Identity=30.9352517985612, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=172, Percent_Identity=31.3953488372093, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=128, Percent_Identity=35.15625, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=179, Percent_Identity=25.6983240223464, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50178; Mature: 50178

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIVSCAMFMEAIDTTILNTAIPVMAHSLEVNPIDLKLVLISYLLSLAIFIPISGWIADK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
YGVKKVFIAAIGVFTLSSLWCGFTQSLWELVLARIIQGLGGSLTVPVGRLIILRTCKRHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHH
LITKMSIVVMVASLGMLLGPLLGGIITDYFSWRWIFWVNIPVGIIAMLLSHKLLPTILPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHHCCC
SVPPLDKSGFIMFGAGLAFLTLGLSLFSETKTDIVYSASITLLSALLLIGYTKHSRNKKN
CCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCC
PIIKVTLLNIRTFRIAVLGNLLTRLSFGGIPFLLPLLFQIILEYSPQLSGMLLTPVALGV
CEEEEEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
FLVKPLSFSILRRLGYKKLLLLNTVLVCFSLWSFATINQSTSVIYIGFLTFLYGFFIALQ
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHH
YTGMNSLAYANIDDNDMSYATSIMSTVQQLSQSFGVAISALLVSLFTSQVSQHFVLTVKI
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHLTFFALGMLTILSGLIFTSLKKEDGKELIESPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFIVSCAMFMEAIDTTILNTAIPVMAHSLEVNPIDLKLVLISYLLSLAIFIPISGWIADK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
YGVKKVFIAAIGVFTLSSLWCGFTQSLWELVLARIIQGLGGSLTVPVGRLIILRTCKRHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHH
LITKMSIVVMVASLGMLLGPLLGGIITDYFSWRWIFWVNIPVGIIAMLLSHKLLPTILPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHHCCC
SVPPLDKSGFIMFGAGLAFLTLGLSLFSETKTDIVYSASITLLSALLLIGYTKHSRNKKN
CCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCC
PIIKVTLLNIRTFRIAVLGNLLTRLSFGGIPFLLPLLFQIILEYSPQLSGMLLTPVALGV
CEEEEEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
FLVKPLSFSILRRLGYKKLLLLNTVLVCFSLWSFATINQSTSVIYIGFLTFLYGFFIALQ
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHH
YTGMNSLAYANIDDNDMSYATSIMSTVQQLSQSFGVAISALLVSLFTSQVSQHFVLTVKI
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHLTFFALGMLTILSGLIFTSLKKEDGKELIESPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]