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The map label for this gene is cmr [H]

Identifier: 148358936

GI number: 148358936

Start: 1615278

End: 1616558

Strand: Reverse

Name: cmr [H]

Synonym: LPC_0821

Alternate gene names: 148358936

Gene position: 1616558-1615278 (Counterclockwise)

Preceding gene: 148358938

Following gene: 148358920

Centisome position: 45.2

GC content: 38.02

Gene sequence:

>1281_bases
ATGTCTGAGCCACTAATTAAAATTTCATACAGACAGGCCATTATCTTTGCTTGCTTCCTGGTTTTATATGAATTTTTGAC
TTATATAGCCAATGACATGATCATGCCAGGTATGATCAATGTTGTTAAATCATTCAACGCGCATGAAAGTGTTGTTGCCA
CCTCTCTAACTGTCTATGTCCTCGGAGGGGCCAGTTTGCAGCTCATACTTGGCCCACTATCAGATGCTTATGGCAGAAGA
CCAATGATGTTAATAGGATCATGCTTATTTTTTCTGTTCACTCTTTTGATTGCAAGCTCACACTCCATGAATCAATTTTT
AATCGCCCGGTTTTTTCAGGGAATGGGATTGTGTTTCATTGGTGTGATAGGTTATGCCACAATTCAAGAAATATTTGAAG
AAATGGACGCTATCCGATTAATTGCTATCATGGCTAATGCCGCAATATTAGCCCCATTATTAGGGCCACTATTAGGAGCC
ATAATCATTCATTATGCGTCCTGGCGTTTGATTTTTATTATTATTGCTTTGGGTGCTCTTTTAGCTTATTGGGGATTATG
GCGGTTTATGCCAGAACCCATAGGCCAGGTAAAAAGAGACGGCCAGCTTATACCTAAAACACCGTTCGCACTGAACGCTA
TAACCCGAAATTATAAAACGTTATTATCTAATCAGGCCTTTTGTTATAGTGCAATTGCTGAAGGTCTGGTAGGGATCCCA
TGCATCGCCTGGATTGCGCTTGCGCCAATCATATTAATTGCTGAAGCAAAATTAACTGTAATTCAATATGGCCTTTGGCA
ACTACCCATCTTCGGGGCAACAATTTTAGGAAACTGGTGCTTACATCATTTAACTTACAAATATAAAATCGAGAGAATTA
TTTTTATAGGTTGTATCATAATGGTCGTGGGACTTGCTCTGACAGCTCTTCTCCCTTATTTTTATGGCAATAATTATGTG
TATTTGATCCCGGGAATCATAATTTACTTTTTCTCACTGAGTGTAATTAATGCCCCCTTGAACAGATATTGTTTATTTGT
AACCGCTGTAAGCAAAGGAACAGCCTCGGCATTAATCAGCCTAAGTATTATGATCATTGGGGCTATTGGAATAGAAATAG
CTAACCTGTTTTATCAACATCACAATAATCTGCATTTCGCCCTCTATTGTAATGCCGTTGGATTATTATTTCTAATCTTT
ATCGGTTTAACTTTTTTTATAGGGACTCCCAAGAAATCTGAAATTCATGATGAAGCAAATAGTAATGTGGCTAACTCATA
A

Upstream 100 bases:

>100_bases
TCGCTTACCAAAACATTTATCAGATTTTCTACCAAATTATGAAATAAACGTATATACTGATAGACCGCCTTTTAGAAACG
ATTGATTCAGGGTTAATGCT

Downstream 100 bases:

>100_bases
GAACAGGCCACATTTACATAACCTGATTCCTTTGTTTTAGGTCAATAACGGAAACATGAACAGGTGAATGGAGTTAACAT
GCATCAAATTGTTGCTATAT

Product: multidrug translocase MdfA

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKSFNAHESVVATSLTVYVLGGASLQLILGPLSDAYGRR
PMMLIGSCLFFLFTLLIASSHSMNQFLIARFFQGMGLCFIGVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGA
IIIHYASWRLIFIIIALGALLAYWGLWRFMPEPIGQVKRDGQLIPKTPFALNAITRNYKTLLSNQAFCYSAIAEGLVGIP
CIAWIALAPIILIAEAKLTVIQYGLWQLPIFGATILGNWCLHHLTYKYKIERIIFIGCIIMVVGLALTALLPYFYGNNYV
YLIPGIIIYFFSLSVINAPLNRYCLFVTAVSKGTASALISLSIMIIGAIGIEIANLFYQHHNNLHFALYCNAVGLLFLIF
IGLTFFIGTPKKSEIHDEANSNVANS

Sequences:

>Translated_426_residues
MSEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKSFNAHESVVATSLTVYVLGGASLQLILGPLSDAYGRR
PMMLIGSCLFFLFTLLIASSHSMNQFLIARFFQGMGLCFIGVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGA
IIIHYASWRLIFIIIALGALLAYWGLWRFMPEPIGQVKRDGQLIPKTPFALNAITRNYKTLLSNQAFCYSAIAEGLVGIP
CIAWIALAPIILIAEAKLTVIQYGLWQLPIFGATILGNWCLHHLTYKYKIERIIFIGCIIMVVGLALTALLPYFYGNNYV
YLIPGIIIYFFSLSVINAPLNRYCLFVTAVSKGTASALISLSIMIIGAIGIEIANLFYQHHNNLHFALYCNAVGLLFLIF
IGLTFFIGTPKKSEIHDEANSNVANS
>Mature_425_residues
SEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKSFNAHESVVATSLTVYVLGGASLQLILGPLSDAYGRRP
MMLIGSCLFFLFTLLIASSHSMNQFLIARFFQGMGLCFIGVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGAI
IIHYASWRLIFIIIALGALLAYWGLWRFMPEPIGQVKRDGQLIPKTPFALNAITRNYKTLLSNQAFCYSAIAEGLVGIPC
IAWIALAPIILIAEAKLTVIQYGLWQLPIFGATILGNWCLHHLTYKYKIERIIFIGCIIMVVGLALTALLPYFYGNNYVY
LIPGIIIYFFSLSVINAPLNRYCLFVTAVSKGTASALISLSIMIIGAIGIEIANLFYQHHNNLHFALYCNAVGLLFLIFI
GLTFFIGTPKKSEIHDEANSNVANS

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=407, Percent_Identity=41.031941031941, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1790794, Length=416, Percent_Identity=32.9326923076923, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI48994889, Length=157, Percent_Identity=33.1210191082803, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788509, Length=248, Percent_Identity=25, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082312, Length=309, Percent_Identity=25.2427184466019, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790146, Length=146, Percent_Identity=25.3424657534247, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322071, Length=204, Percent_Identity=22.0588235294118, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319480, Length=192, Percent_Identity=27.0833333333333, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321838, Length=159, Percent_Identity=31.4465408805031, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=222, Percent_Identity=22.0720720720721, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47142; Mature: 47011

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKSFNAHESVVATSLTVYV
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHEEEE
LGGASLQLILGPLSDAYGRRPMMLIGSCLFFLFTLLIASSHSMNQFLIARFFQGMGLCFI
ECCCEEEEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
GVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGAIIIHYASWRLIFIIIALGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYWGLWRFMPEPIGQVKRDGQLIPKTPFALNAITRNYKTLLSNQAFCYSAIAEGLVGIP
HHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
CIAWIALAPIILIAEAKLTVIQYGLWQLPIFGATILGNWCLHHLTYKYKIERIIFIGCII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVVGLALTALLPYFYGNNYVYLIPGIIIYFFSLSVINAPLNRYCLFVTAVSKGTASALIS
HHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCHHHHEEEEEEECCCHHHHHHH
LSIMIIGAIGIEIANLFYQHHNNLHFALYCNAVGLLFLIFIGLTFFIGTPKKSEIHDEAN
HHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHC
SNVANS
CCCCCC
>Mature Secondary Structure 
SEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKSFNAHESVVATSLTVYV
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHEEEE
LGGASLQLILGPLSDAYGRRPMMLIGSCLFFLFTLLIASSHSMNQFLIARFFQGMGLCFI
ECCCEEEEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
GVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGAIIIHYASWRLIFIIIALGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYWGLWRFMPEPIGQVKRDGQLIPKTPFALNAITRNYKTLLSNQAFCYSAIAEGLVGIP
HHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
CIAWIALAPIILIAEAKLTVIQYGLWQLPIFGATILGNWCLHHLTYKYKIERIIFIGCII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVVGLALTALLPYFYGNNYVYLIPGIIIYFFSLSVINAPLNRYCLFVTAVSKGTASALIS
HHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCHHHHEEEEEEECCCHHHHHHH
LSIMIIGAIGIEIANLFYQHHNNLHFALYCNAVGLLFLIFIGLTFFIGTPKKSEIHDEAN
HHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHC
SNVANS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]