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The map label for this gene is holB [H]

Identifier: 148358931

GI number: 148358931

Start: 1609982

End: 1610887

Strand: Direct

Name: holB [H]

Synonym: LPC_0816

Alternate gene names: 148358931

Gene position: 1609982-1610887 (Clockwise)

Preceding gene: 148358930

Following gene: 148358932

Centisome position: 45.02

GC content: 35.21

Gene sequence:

>906_bases
ATGATTACTTATCAAGAACAATGGCAGAAAATGCAAATGGCCTTGGCGATAAGACGAATTCCTCAAGCCATGCTATTTTT
GGGTTCTTTGCATTGTGGTCTTATAGAATTTACAAATCAAGTGATCAAATTACTCTTGTGTAAAATAAAACAGAATGAGC
CTTGTTTGACTTGCATGGATTGCCAAATGATAGAGCGTTTTGAGCATCCGGATGTGGAATGGATTAAGCCTGAAAGAAGC
GGTGGTATTATTAAAATTGATCAAATTAGGGGATTGCAGAGTACGGCGTTTCTAACACCACAGAGGACTAATCATAAATT
TATAATTATCGAATCAGCTGAATCAATGAATATTGCGGCATCAAATGCCTTATTGAAAATTCTGGAAGAGCCAGCGCAAC
ATACTATCTTTATTTTGTTAGCGCAACAAATTGGCAATATACCACCCACCGTATTGAGCCGATGCCAAATAACGCAATTT
GCTGTTTTTGATGAGTTGCCTTGCAACAATTTATTGGCTTTAGGAGAGTACTATACTCGGGAATTGAATAGCACAATGGT
CTTCAAGCATTCAGAATCTATTTTAGAAGGCTTGATTGCAATTATTGAGCGAAGAGAGCATCCATGCACTTTGGCTGCAC
AATGGATTCAACATGAATTAAGTGCTTTTTTGTGGTTTTTATATTTGCTCTATTCTCAACTACAGTATATGCATTTGTTT
CGAGTGGCTTATTCTGGTACGTTAACAAAACAATTGAATAAGCTATCCTCATTACTCAATCCGGTTATGATTTTTGCCCA
AGTAGATAAAATCAATACTTTGTTAAGAAAATTAAGCCATAATATGAATATGAATCATACATTGGTCTTGGAAGATTTAT
TATTTTCTTTGGTTGTTGATTATTAA

Upstream 100 bases:

>100_bases
AACAAAACCCGGAAATTATGATGATTGATGCTAACAGGCCTTTGGAAGATGTTCAATCTTCCATTCAATCAGTAATCGAG
GAGTTTATAGAGCATAACCT

Downstream 100 bases:

>100_bases
TTGAGTTTAAAGGAATTAAACCATTGCGTGTGAACCGAGTTTTTTATTAAGCAACTGAAGTGAGCAAGATGATTAGTTAA
AACAGGACTATTCAAGGAGG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MITYQEQWQKMQMALAIRRIPQAMLFLGSLHCGLIEFTNQVIKLLLCKIKQNEPCLTCMDCQMIERFEHPDVEWIKPERS
GGIIKIDQIRGLQSTAFLTPQRTNHKFIIIESAESMNIAASNALLKILEEPAQHTIFILLAQQIGNIPPTVLSRCQITQF
AVFDELPCNNLLALGEYYTRELNSTMVFKHSESILEGLIAIIERREHPCTLAAQWIQHELSAFLWFLYLLYSQLQYMHLF
RVAYSGTLTKQLNKLSSLLNPVMIFAQVDKINTLLRKLSHNMNMNHTLVLEDLLFSLVVDY

Sequences:

>Translated_301_residues
MITYQEQWQKMQMALAIRRIPQAMLFLGSLHCGLIEFTNQVIKLLLCKIKQNEPCLTCMDCQMIERFEHPDVEWIKPERS
GGIIKIDQIRGLQSTAFLTPQRTNHKFIIIESAESMNIAASNALLKILEEPAQHTIFILLAQQIGNIPPTVLSRCQITQF
AVFDELPCNNLLALGEYYTRELNSTMVFKHSESILEGLIAIIERREHPCTLAAQWIQHELSAFLWFLYLLYSQLQYMHLF
RVAYSGTLTKQLNKLSSLLNPVMIFAQVDKINTLLRKLSHNMNMNHTLVLEDLLFSLVVDY
>Mature_301_residues
MITYQEQWQKMQMALAIRRIPQAMLFLGSLHCGLIEFTNQVIKLLLCKIKQNEPCLTCMDCQMIERFEHPDVEWIKPERS
GGIIKIDQIRGLQSTAFLTPQRTNHKFIIIESAESMNIAASNALLKILEEPAQHTIFILLAQQIGNIPPTVLSRCQITQF
AVFDELPCNNLLALGEYYTRELNSTMVFKHSESILEGLIAIIERREHPCTLAAQWIQHELSAFLWFLYLLYSQLQYMHLF
RVAYSGTLTKQLNKLSSLLNPVMIFAQVDKINTLLRKLSHNMNMNHTLVLEDLLFSLVVDY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=143, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 34782; Mature: 34782

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITYQEQWQKMQMALAIRRIPQAMLFLGSLHCGLIEFTNQVIKLLLCKIKQNEPCLTCMD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHH
CQMIERFEHPDVEWIKPERSGGIIKIDQIRGLQSTAFLTPQRTNHKFIIIESAESMNIAA
HHHHHHCCCCCCCEECCCCCCCEEEEHHHCCCCHHEEECCCCCCCEEEEEECCCCCCHHH
SNALLKILEEPAQHTIFILLAQQIGNIPPTVLSRCQITQFAVFDELPCNNLLALGEYYTR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ELNSTMVFKHSESILEGLIAIIERREHPCTLAAQWIQHELSAFLWFLYLLYSQLQYMHLF
HHCCCEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVAYSGTLTKQLNKLSSLLNPVMIFAQVDKINTLLRKLSHNMNMNHTLVLEDLLFSLVVD
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
Y
C
>Mature Secondary Structure
MITYQEQWQKMQMALAIRRIPQAMLFLGSLHCGLIEFTNQVIKLLLCKIKQNEPCLTCMD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHH
CQMIERFEHPDVEWIKPERSGGIIKIDQIRGLQSTAFLTPQRTNHKFIIIESAESMNIAA
HHHHHHCCCCCCCEECCCCCCCEEEEHHHCCCCHHEEECCCCCCCEEEEEECCCCCCHHH
SNALLKILEEPAQHTIFILLAQQIGNIPPTVLSRCQITQFAVFDELPCNNLLALGEYYTR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ELNSTMVFKHSESILEGLIAIIERREHPCTLAAQWIQHELSAFLWFLYLLYSQLQYMHLF
HHCCCEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVAYSGTLTKQLNKLSSLLNPVMIFAQVDKINTLLRKLSHNMNMNHTLVLEDLLFSLVVD
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]