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The map label for this gene is lspF [H]

Identifier: 148358894

GI number: 148358894

Start: 1572887

End: 1574086

Strand: Reverse

Name: lspF [H]

Synonym: LPC_0779

Alternate gene names: 148358894

Gene position: 1574086-1572887 (Counterclockwise)

Preceding gene: 148358901

Following gene: 148358893

Centisome position: 44.01

GC content: 40.33

Gene sequence:

>1200_bases
ATGGGCGCCTACCAATATCAAGCGCTAAAAGTGAATGGCAGCATTAGCAAAGGTGTAATAGAAGCTGATTCTGAACGTCA
TGCACGTCAATTATTGCGAGAACAAGGACTAATCCCTACTCAAGTCCAAACATTAACCCAGCAACGTGCTGCCAGCAGCA
AAAGTAAAGTTTCTGCGGCTGATTTGGCATTACTCACCAGACAGCTGGCAACACTTCTAGCTGCCGGTATTCCTGTTGAA
GAATCATTGCGCGGGGTCAGCGAACAAACAGAAAAAGACAAAGTCAGAGAGCTTATTATCGGAGTTCGCTCTAAAGTATT
GGAAGGGTATGGTCTTGCTCAGGCAATGGCTCAATATCCCAATGCCTTTCCAGAACTATACCGGGCAACAGTAGGCTCAG
GCGAGCAGACAGGGCGTCTTGATGTTGTTTTAGAAAAACTGGCTGATTATACTGAAAAACAACAACAAACTCGACAGAAA
GTGCAGCAAGCTTTGATTTACCCATTGCTCATGATCATTGTTTCTACTGCCATTATCAGTTTTTTACTTACTTTTGTTGT
CCCTAAAATTATTGATGTATTTACCGAAAGTGGCCAAACACTACCACCAATGACGCAATTGCTTATTAACTTGAGCCAAT
TTATTAAATCTTATGGTTTGTATAGTCTAGTGACAATGATTGTGGCTCTAATTGGCTTTAAAAAGAGCCTCAGTAATATA
AAAATTAAAACAGCATGGCATCAATTCATGCTTAAACTACCTATCGTGTCTTATTTGGTTAAAACAATTAATGTCGCGCG
ATATATTCACACGTTTGGAATTCTATTTGCAGCGGGAGTCAGTGTACTTGAAACCATGCGTGTTTCTTCAAGTTTGGTAA
CCAATATCGTGATGAGACAGGCTTTTGATTTAGCTACTCTACGAGTCCGTGAAGGAAGTGGAATCAGTGAGGCCCTCAAA
GAAACAAAATTCATTAGCCCCATGGCTATCCATCTGATAGCCAGTGGTGAGAAAAGCGGCCAACTTTCAGACATGATGGA
ACGCTCGGCGTCTCATTTAGATAATGAAGTAAACCGACTGATTGAAACATCCTTAACTTTATTAGAACCTATGGTTATTC
TATTAATGGGTGCTGTTGTTTTATTTATTGTACTAGCGACCTTATTACCTATTTTTTCAATGGAACAATTAGTTGCCTGA

Upstream 100 bases:

>100_bases
TCATCTTTTGTCTCCAGGATAGTCCGCGTATAGTTTACCTTAAGTGACTTATTAATCCCAATAGATTACACTCTATAGTT
TTAATTTTAACAAAAATAAT

Downstream 100 bases:

>100_bases
TTAAGCGGAGAAAGGGCTATCCGCGTCTTTTTAAAATTTAAGTCTATTCATAGGCATCAATATTTGTGTTATAATTTAAC
ATTTAGTTTCAAGAGGTACA

Product: type II secretory pathway protein LspF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MGAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKSKVSAADLALLTRQLATLLAAGIPVE
ESLRGVSEQTEKDKVRELIIGVRSKVLEGYGLAQAMAQYPNAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQK
VQQALIYPLLMIIVSTAIISFLLTFVVPKIIDVFTESGQTLPPMTQLLINLSQFIKSYGLYSLVTMIVALIGFKKSLSNI
KIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILFAAGVSVLETMRVSSSLVTNIVMRQAFDLATLRVREGSGISEALK
ETKFISPMAIHLIASGEKSGQLSDMMERSASHLDNEVNRLIETSLTLLEPMVILLMGAVVLFIVLATLLPIFSMEQLVA

Sequences:

>Translated_399_residues
MGAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKSKVSAADLALLTRQLATLLAAGIPVE
ESLRGVSEQTEKDKVRELIIGVRSKVLEGYGLAQAMAQYPNAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQK
VQQALIYPLLMIIVSTAIISFLLTFVVPKIIDVFTESGQTLPPMTQLLINLSQFIKSYGLYSLVTMIVALIGFKKSLSNI
KIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILFAAGVSVLETMRVSSSLVTNIVMRQAFDLATLRVREGSGISEALK
ETKFISPMAIHLIASGEKSGQLSDMMERSASHLDNEVNRLIETSLTLLEPMVILLMGAVVLFIVLATLLPIFSMEQLVA
>Mature_398_residues
GAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKSKVSAADLALLTRQLATLLAAGIPVEE
SLRGVSEQTEKDKVRELIIGVRSKVLEGYGLAQAMAQYPNAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQKV
QQALIYPLLMIIVSTAIISFLLTFVVPKIIDVFTESGQTLPPMTQLLINLSQFIKSYGLYSLVTMIVALIGFKKSLSNIK
IKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILFAAGVSVLETMRVSSSLVTNIVMRQAFDLATLRVREGSGISEALKE
TKFISPMAIHLIASGEKSGQLSDMMERSASHLDNEVNRLIETSLTLLEPMVILLMGAVVLFIVLATLLPIFSMEQLVA

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=395, Percent_Identity=36.7088607594937, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1786295, Length=406, Percent_Identity=26.8472906403941, Blast_Score=135, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43959; Mature: 43828

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKSKVSAA
CCCCEEEEEEECCCHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
DLALLTRQLATLLAAGIPVEESLRGVSEQTEKDKVRELIIGVRSKVLEGYGLAQAMAQYP
HHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
NAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQKVQQALIYPLLMIIVSTAIIS
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLLTFVVPKIIDVFTESGQTLPPMTQLLINLSQFIKSYGLYSLVTMIVALIGFKKSLSNI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
KIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILFAAGVSVLETMRVSSSLVTNIVMRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFDLATLRVREGSGISEALKETKFISPMAIHLIASGEKSGQLSDMMERSASHLDNEVNRL
HHHHHHHEEECCCCHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
IETSLTLLEPMVILLMGAVVLFIVLATLLPIFSMEQLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
GAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKSKVSAA
CCCEEEEEEECCCHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
DLALLTRQLATLLAAGIPVEESLRGVSEQTEKDKVRELIIGVRSKVLEGYGLAQAMAQYP
HHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
NAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQKVQQALIYPLLMIIVSTAIIS
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLLTFVVPKIIDVFTESGQTLPPMTQLLINLSQFIKSYGLYSLVTMIVALIGFKKSLSNI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
KIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILFAAGVSVLETMRVSSSLVTNIVMRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFDLATLRVREGSGISEALKETKFISPMAIHLIASGEKSGQLSDMMERSASHLDNEVNRL
HHHHHHHEEECCCCHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
IETSLTLLEPMVILLMGAVVLFIVLATLLPIFSMEQLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]