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The map label for this gene is kdsA

Identifier: 148358769

GI number: 148358769

Start: 1430263

End: 1431087

Strand: Direct

Name: kdsA

Synonym: LPC_0649

Alternate gene names: 148358769

Gene position: 1430263-1431087 (Clockwise)

Preceding gene: 148358768

Following gene: 148358770

Centisome position: 39.99

GC content: 41.45

Gene sequence:

>825_bases
ATGAGATTATGTGGTTTTGAAGCAGGCTTGGATAAGCCATTGTTTTTGATTGCCGGCCCTTGTGTTATTGAAAGTGAAGA
ACTGGCTTTAGAGACCGCAGGATATTTAAAGGAAATGTGCAGTCAATTGAATATCCCTTTCATCTATAAGTCTTCTTTTG
ATAAAGCAAACCGTTCATCTATTTCCAGTTACAGGGGGCCTGGCTTTGAAAAAGGGTTATCGATTCTGGAAAAGGTCAAA
TCACAAATAGGTGTTCCTGTTTTGACTGATGTTCATGAAGACACGCCCTTATTTGAAGTCTCCAGTGTGGTAGATGTATT
ACAAACGCCAGCTTTCCTATGCAGACAAACCAATTTTATCCAAAAAGTTGCTGCTATGAATAAGCCGGTTAATATTAAAA
AGGGACAGTTTCTTGCTCCTTGGGAAATGAAACATGTGATTGCGAAGGCAAAGGCGCAAGGCAATGAGCAAATTATGGCT
TGTGAGCGAGGAGTGAGCTTCGGCTACAATAATTTGGTTTCTGATATGCGTTCCCTCGTTATCATGCGTGAAACCGGCTG
TCCAGTGGTTTATGATGCAACACATTCGGTTCAATTGCCCGGGGGCAATAATGGTGTATCCGGAGGGCAAAGGGAATTTA
TTCCGGCTCTTGCAAGAGCAGCTGTAGCTGTTGGAATTTCAGGGTTATTTATGGAAACTCACCCTGATCCGGATAAAGCA
CTAAGTGATGGTCCCAATAGTTGGCCTCTAGATAAAATGAAACAACTTTTGGAATCATTGAAAGCAGCCGATGAAGTGTA
TAAAAAGTACAGTACTGATTTCTAA

Upstream 100 bases:

>100_bases
AGTTTCATCCTGAATTCACATCGAATCCAAGAGATAGTCATCCTTTGTTTAAGGAATTTGTACTTGCAGCTCGTATTCAC
CATCAAGAGAAGGACAAAAA

Downstream 100 bases:

>100_bases
ATTAAATGATATCCCGTGTAAAAAGAAGTATGGCACGGGAATATTTTTTATTTTGCTCCTCCCTTTTATCTGATTAATCA
TACCGTTTTTATTTGATTCA

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MRLCGFEAGLDKPLFLIAGPCVIESEELALETAGYLKEMCSQLNIPFIYKSSFDKANRSSISSYRGPGFEKGLSILEKVK
SQIGVPVLTDVHEDTPLFEVSSVVDVLQTPAFLCRQTNFIQKVAAMNKPVNIKKGQFLAPWEMKHVIAKAKAQGNEQIMA
CERGVSFGYNNLVSDMRSLVIMRETGCPVVYDATHSVQLPGGNNGVSGGQREFIPALARAAVAVGISGLFMETHPDPDKA
LSDGPNSWPLDKMKQLLESLKAADEVYKKYSTDF

Sequences:

>Translated_274_residues
MRLCGFEAGLDKPLFLIAGPCVIESEELALETAGYLKEMCSQLNIPFIYKSSFDKANRSSISSYRGPGFEKGLSILEKVK
SQIGVPVLTDVHEDTPLFEVSSVVDVLQTPAFLCRQTNFIQKVAAMNKPVNIKKGQFLAPWEMKHVIAKAKAQGNEQIMA
CERGVSFGYNNLVSDMRSLVIMRETGCPVVYDATHSVQLPGGNNGVSGGQREFIPALARAAVAVGISGLFMETHPDPDKA
LSDGPNSWPLDKMKQLLESLKAADEVYKKYSTDF
>Mature_274_residues
MRLCGFEAGLDKPLFLIAGPCVIESEELALETAGYLKEMCSQLNIPFIYKSSFDKANRSSISSYRGPGFEKGLSILEKVK
SQIGVPVLTDVHEDTPLFEVSSVVDVLQTPAFLCRQTNFIQKVAAMNKPVNIKKGQFLAPWEMKHVIAKAKAQGNEQIMA
CERGVSFGYNNLVSDMRSLVIMRETGCPVVYDATHSVQLPGGNNGVSGGQREFIPALARAAVAVGISGLFMETHPDPDKA
LSDGPNSWPLDKMKQLLESLKAADEVYKKYSTDF

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=262, Percent_Identity=44.2748091603053, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_LEGPA (Q5X5Y5)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123509.1
- ProteinModelPortal:   Q5X5Y5
- SMR:   Q5X5Y5
- STRING:   Q5X5Y5
- GeneID:   3116950
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp1185
- LegioList:   lpp1185
- eggNOG:   COG2877
- HOGENOM:   HBG575990
- OMA:   GVFLECH
- ProtClustDB:   PRK05198
- BioCyc:   LPNE297246:LPP1185-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 29984; Mature: 29984

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLCGFEAGLDKPLFLIAGPCVIESEELALETAGYLKEMCSQLNIPFIYKSSFDKANRSS
CCCCCCCCCCCCCEEEEECCCEECCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHH
ISSYRGPGFEKGLSILEKVKSQIGVPVLTDVHEDTPLFEVSSVVDVLQTPAFLCRQTNFI
HHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHH
QKVAAMNKPVNIKKGQFLAPWEMKHVIAKAKAQGNEQIMACERGVSFGYNNLVSDMRSLV
HHHHHCCCCCCCCCCCEECCHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHH
IMRETGCPVVYDATHSVQLPGGNNGVSGGQREFIPALARAAVAVGISGLFMETHPDPDKA
HHHCCCCCEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCHHHH
LSDGPNSWPLDKMKQLLESLKAADEVYKKYSTDF
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRLCGFEAGLDKPLFLIAGPCVIESEELALETAGYLKEMCSQLNIPFIYKSSFDKANRSS
CCCCCCCCCCCCCEEEEECCCEECCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHH
ISSYRGPGFEKGLSILEKVKSQIGVPVLTDVHEDTPLFEVSSVVDVLQTPAFLCRQTNFI
HHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHH
QKVAAMNKPVNIKKGQFLAPWEMKHVIAKAKAQGNEQIMACERGVSFGYNNLVSDMRSLV
HHHHHCCCCCCCCCCCEECCHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHH
IMRETGCPVVYDATHSVQLPGGNNGVSGGQREFIPALARAAVAVGISGLFMETHPDPDKA
HHHCCCCCEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCHHHH
LSDGPNSWPLDKMKQLLESLKAADEVYKKYSTDF
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA