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The map label for this gene is pilR [H]

Identifier: 148358761

GI number: 148358761

Start: 1421353

End: 1422681

Strand: Direct

Name: pilR [H]

Synonym: LPC_0640

Alternate gene names: 148358761

Gene position: 1421353-1422681 (Clockwise)

Preceding gene: 148358760

Following gene: 148358762

Centisome position: 39.74

GC content: 40.33

Gene sequence:

>1329_bases
ATGACCAAAAGTAAAGTGCTTGTTATAGATGACGAACCAGATATAAGAGAGTTATTAACTCTTACTTTATCGCGTATGGG
TTTAGCTTGCGATGCAGCGTCTGACTTTAAGCAAGGTGTAGAACATATAAAACAAAATTATTATTCTTTGGTTTTAACCG
ATATGCGTTTGCCTGATGGAGATGGTATAGAAATTGTTAAATTTATTCAGAAATACAAACCACAGGTGCCAGTTGCTGTC
ATTACTGCCTATGGAAATGTCGAGGGTGCAGTCAATACTTTAAAGGCGGGTGCATTTGATTATGTATCAAAACCTATAGA
CTTAAAAATGCTTAGAGATCTGGTTAAGACGGCATTATCCATGCAAAACCCGTCTACCGAGAGGCCTGATGAGAGTCTAT
TGGTTGGTGAAAGTTTGGTCATGCAACAACTTCGTGAAAGCATTCATAAATTAGCTCGCAGTCAGGCACCGGTTTTTATT
CAAGGGGATTCAGGTGTTGGAAAAGAACTGGTCGCTCAGTTGATCCATGCTAATGGTCCCAGAAAGGATAAGCCATTTAT
TCCTGTAAACTGTGGCGCTATACCTGGTGAGTTGATGGAGTCAGAGTTTTTTGGCCATAAAAAAGGGAGCTTTACAGGAG
CGATTTCTGATAAAACAGGGCTGTTTGTTGCGGCTCATGGTGGCACTTTGTTTCTTGATGAAATCGCCGAACTTCCTCTC
GCCATGCAGGTTAAATTGTTACGTGCTATTCAGGAGAAGGCAATTAAGCCGATAGGTGAGTTATATGAAGTTCCTGTGGA
TGTTCGCATCCTAAGTGCGAGTCATAAAAATTTGATTGAAGAAGTTAATGCCGGGATGTTTCGTCAAGATTTGTATTATC
GAATTAATGTGATTGAATTGCGTGTTCCAACTTTAATGGAAAGGCTTTCAGATATTCCTTGCCTGACAGAGGCGATATTA
AGAAAATTATCTAAAAATCAAAATAGAAACCTGGTGAAAATAAATCCATCCTGTTTTGAAATATTGAGCCGTTATCATTT
TCCAGGAAATGTCAGAGAACTGGAGAATATTCTCGAGCGTGCCATGGCTATGTGTGAAGGAGAGGTTATAGAACCATCCG
ATTTACATTTGCCCAGAACAACAATAACCCCAGTTGTTGCCGTATCCCCCGCGTCGACTAACTTAAATGGTTACCTTCAG
GATCAGGAAAAAGAGTTAATTCTTGATGCGCTTGAGAAAACAAAATGGAACAGAACCGCTGCTGCTCAACTACTGGGGGT
CAGCTTCCGAACTCTCCGTTATCGATTAAAAAAACTGGGTCTTGATTAG

Upstream 100 bases:

>100_bases
GAGATTTGTGTGAAATTAATCAAGCCAGATTAAATTTGGTAAAATCAAATAAAGGATGTTGTTTTGCGATCACTGTTAAT
CCATCGGATGAACTTTTAAT

Downstream 100 bases:

>100_bases
ATGTTGCCCTTTGAAGATTTTCAAAGGTTAACATAGATCTCCTGCCTGGCCAGCCTCTTTTGTTTTATTGCTGCCCTGAA
AACGTTTCTGAGGTGCTCAT

Product: two component response regulator PilR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTKSKVLVIDDEPDIRELLTLTLSRMGLACDAASDFKQGVEHIKQNYYSLVLTDMRLPDGDGIEIVKFIQKYKPQVPVAV
ITAYGNVEGAVNTLKAGAFDYVSKPIDLKMLRDLVKTALSMQNPSTERPDESLLVGESLVMQQLRESIHKLARSQAPVFI
QGDSGVGKELVAQLIHANGPRKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPL
AMQVKLLRAIQEKAIKPIGELYEVPVDVRILSASHKNLIEEVNAGMFRQDLYYRINVIELRVPTLMERLSDIPCLTEAIL
RKLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILERAMAMCEGEVIEPSDLHLPRTTITPVVAVSPASTNLNGYLQ
DQEKELILDALEKTKWNRTAAAQLLGVSFRTLRYRLKKLGLD

Sequences:

>Translated_442_residues
MTKSKVLVIDDEPDIRELLTLTLSRMGLACDAASDFKQGVEHIKQNYYSLVLTDMRLPDGDGIEIVKFIQKYKPQVPVAV
ITAYGNVEGAVNTLKAGAFDYVSKPIDLKMLRDLVKTALSMQNPSTERPDESLLVGESLVMQQLRESIHKLARSQAPVFI
QGDSGVGKELVAQLIHANGPRKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPL
AMQVKLLRAIQEKAIKPIGELYEVPVDVRILSASHKNLIEEVNAGMFRQDLYYRINVIELRVPTLMERLSDIPCLTEAIL
RKLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILERAMAMCEGEVIEPSDLHLPRTTITPVVAVSPASTNLNGYLQ
DQEKELILDALEKTKWNRTAAAQLLGVSFRTLRYRLKKLGLD
>Mature_441_residues
TKSKVLVIDDEPDIRELLTLTLSRMGLACDAASDFKQGVEHIKQNYYSLVLTDMRLPDGDGIEIVKFIQKYKPQVPVAVI
TAYGNVEGAVNTLKAGAFDYVSKPIDLKMLRDLVKTALSMQNPSTERPDESLLVGESLVMQQLRESIHKLARSQAPVFIQ
GDSGVGKELVAQLIHANGPRKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPLA
MQVKLLRAIQEKAIKPIGELYEVPVDVRILSASHKNLIEEVNAGMFRQDLYYRINVIELRVPTLMERLSDIPCLTEAILR
KLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILERAMAMCEGEVIEPSDLHLPRTTITPVVAVSPASTNLNGYLQD
QEKELILDALEKTKWNRTAAAQLLGVSFRTLRYRLKKLGLD

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=441, Percent_Identity=41.7233560090703, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1788550, Length=455, Percent_Identity=39.3406593406593, Blast_Score=334, Evalue=8e-93,
Organism=Escherichia coli, GI1788905, Length=436, Percent_Identity=37.6146788990826, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1790299, Length=475, Percent_Identity=35.5789473684211, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1789233, Length=336, Percent_Identity=41.9642857142857, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI87082117, Length=321, Percent_Identity=38.3177570093458, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1786524, Length=319, Percent_Identity=41.3793103448276, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082152, Length=319, Percent_Identity=40.1253918495298, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1789087, Length=314, Percent_Identity=37.8980891719745, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1787583, Length=333, Percent_Identity=36.036036036036, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI87081872, Length=315, Percent_Identity=35.2380952380952, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87081858, Length=305, Percent_Identity=32.4590163934426, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789828, Length=259, Percent_Identity=33.2046332046332, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786599, Length=122, Percent_Identity=31.9672131147541, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790552, Length=132, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786911, Length=127, Percent_Identity=32.2834645669291, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786784, Length=115, Percent_Identity=33.0434782608696, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082012, Length=115, Percent_Identity=34.7826086956522, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789149, Length=112, Percent_Identity=32.1428571428571, Blast_Score=62, Evalue=8e-11,
Organism=Escherichia coli, GI145693157, Length=116, Percent_Identity=30.1724137931034, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49232; Mature: 49101

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSKVLVIDDEPDIRELLTLTLSRMGLACDAASDFKQGVEHIKQNYYSLVLTDMRLPDG
CCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCC
DGIEIVKFIQKYKPQVPVAVITAYGNVEGAVNTLKAGAFDYVSKPIDLKMLRDLVKTALS
CCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHH
MQNPSTERPDESLLVGESLVMQQLRESIHKLARSQAPVFIQGDSGVGKELVAQLIHANGP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCC
RKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPL
CCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCEEEHHHHHHCCH
AMQVKLLRAIQEKAIKPIGELYEVPVDVRILSASHKNLIEEVNAGMFRQDLYYRINVIEL
HHHHHHHHHHHHHHHCHHHHHHHCCCCEEEECCHHHHHHHHHHCCHHHHHHEEEEEEEEE
RVPTLMERLSDIPCLTEAILRKLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILER
EHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHH
AMAMCEGEVIEPSDLHLPRTTITPVVAVSPASTNLNGYLQDQEKELILDALEKTKWNRTA
HHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEECCHHHHHHHHHHHHCCCCHHH
AAQLLGVSFRTLRYRLKKLGLD
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKSKVLVIDDEPDIRELLTLTLSRMGLACDAASDFKQGVEHIKQNYYSLVLTDMRLPDG
CCCCEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCC
DGIEIVKFIQKYKPQVPVAVITAYGNVEGAVNTLKAGAFDYVSKPIDLKMLRDLVKTALS
CCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHH
MQNPSTERPDESLLVGESLVMQQLRESIHKLARSQAPVFIQGDSGVGKELVAQLIHANGP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCC
RKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPL
CCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCEEEHHHHHHCCH
AMQVKLLRAIQEKAIKPIGELYEVPVDVRILSASHKNLIEEVNAGMFRQDLYYRINVIEL
HHHHHHHHHHHHHHHCHHHHHHHCCCCEEEECCHHHHHHHHHHCCHHHHHHEEEEEEEEE
RVPTLMERLSDIPCLTEAILRKLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILER
EHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHH
AMAMCEGEVIEPSDLHLPRTTITPVVAVSPASTNLNGYLQDQEKELILDALEKTKWNRTA
HHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEECCHHHHHHHHHHHHCCCCHHH
AAQLLGVSFRTLRYRLKKLGLD
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]