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The map label for this gene is potB [H]

Identifier: 148358725

GI number: 148358725

Start: 1378201

End: 1379025

Strand: Reverse

Name: potB [H]

Synonym: LPC_0604

Alternate gene names: 148358725

Gene position: 1379025-1378201 (Counterclockwise)

Preceding gene: 148358726

Following gene: 148358724

Centisome position: 38.56

GC content: 37.82

Gene sequence:

>825_bases
ATGAAGGCTAAATCATCCGCACTGTATTTATTGTATTTTTGGTTAATTATTTTTGGCTTCATTCCCTTGTTGATGGTGCT
GGTTGCCAGCTTTTTATCACAAGACAGTATTCATCTTGTTACCTTGCCATTGACATTGGAAAATTATTTTGACTTGTTTA
CTCCTGTTTTTGCAAAAATCCTTTTCCGATCCTTACTGATAGCCTGTGTCACCACCCTGCTTTGTCTGCTCATCGCATAC
CCCTTTAGCTATCTAATGATTAAATCAAAACACCAATCTATTTTATTGCTGTTGATTATCATACCTTTCTGGACAAGCTC
ATTGATAAGAACTTATGCTTTGATTGCTATCCTTAAATACAAAGGCGTGATAAATGCCCTATTGCTGAAATTACACTTGA
TTGATGTTCCCTTGTCACTACTTTATACCAATTTTGCGGTTGTTATTGGCTTGGTTTACAATCTTTTCCCTTTCATGGTC
CTACCGATTTTTACTAATATGGAACGGTTCGATTTCAGGTTAATTGAGGCAGCTAAAGATTTAGGGGCAAACAAGTGGTC
CATATTTACACGAGTATTTTTGCCTAACACAGCCCCTGGAATCTTATCAGGTTGTTTGCTGGTCTTGTTACCAGCAATGA
CTTTATTTTACATTCCTAATGTATTAGGTGGCGCTCGTTCCATTTTATTGGGAAATTTAATCCAAAATCAATTCCTTGTC
CTGAGCGATTGGCCTCAGGGAGCCGCTACCAGTGTGCTATTAACTTCCGTTCTGCTTATTTTAATGTTCATTTTTCGGCG
ACAGAACAATGAGGTCATTCGATGA

Upstream 100 bases:

>100_bases
GCTTCGGAGTTTTTTGATGAAGACGATGATAAGCTGGAATACACTCTCCATGAAACCGTTTGGGTACAATGGCTGCCTGG
CTGGGAGGTTTTATTACCTT

Downstream 100 bases:

>100_bases
AAACAATGACACAGCGCCTGTTTCTTTCTTTAGTTTATGCTTTTCTTTATATCCCCATTGCAATACTGGTGCTTTATTCA
GTAAATGATGCCAAATTTTC

Product: spermidine/putrescine ABC transporter permease PotB

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPLTLENYFDLFTPVFAKILFRSLLIACVTTLLCLLIAY
PFSYLMIKSKHQSILLLLIIIPFWTSSLIRTYALIAILKYKGVINALLLKLHLIDVPLSLLYTNFAVVIGLVYNLFPFMV
LPIFTNMERFDFRLIEAAKDLGANKWSIFTRVFLPNTAPGILSGCLLVLLPAMTLFYIPNVLGGARSILLGNLIQNQFLV
LSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR

Sequences:

>Translated_274_residues
MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPLTLENYFDLFTPVFAKILFRSLLIACVTTLLCLLIAY
PFSYLMIKSKHQSILLLLIIIPFWTSSLIRTYALIAILKYKGVINALLLKLHLIDVPLSLLYTNFAVVIGLVYNLFPFMV
LPIFTNMERFDFRLIEAAKDLGANKWSIFTRVFLPNTAPGILSGCLLVLLPAMTLFYIPNVLGGARSILLGNLIQNQFLV
LSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR
>Mature_274_residues
MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPLTLENYFDLFTPVFAKILFRSLLIACVTTLLCLLIAY
PFSYLMIKSKHQSILLLLIIIPFWTSSLIRTYALIAILKYKGVINALLLKLHLIDVPLSLLYTNFAVVIGLVYNLFPFMV
LPIFTNMERFDFRLIEAAKDLGANKWSIFTRVFLPNTAPGILSGCLLVLLPAMTLFYIPNVLGGARSILLGNLIQNQFLV
LSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=259, Percent_Identity=46.7181467181467, Blast_Score=246, Evalue=9e-67,
Organism=Escherichia coli, GI1787080, Length=236, Percent_Identity=41.9491525423729, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1787713, Length=271, Percent_Identity=28.7822878228782, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31029; Mature: 31029

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPLTLENYFDLFTPVFAKI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHH
LFRSLLIACVTTLLCLLIAYPFSYLMIKSKHQSILLLLIIIPFWTSSLIRTYALIAILKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGVINALLLKLHLIDVPLSLLYTNFAVVIGLVYNLFPFMVLPIFTNMERFDFRLIEAAKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
LGANKWSIFTRVFLPNTAPGILSGCLLVLLPAMTLFYIPNVLGGARSILLGNLIQNQFLV
CCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPLTLENYFDLFTPVFAKI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHH
LFRSLLIACVTTLLCLLIAYPFSYLMIKSKHQSILLLLIIIPFWTSSLIRTYALIAILKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGVINALLLKLHLIDVPLSLLYTNFAVVIGLVYNLFPFMVLPIFTNMERFDFRLIEAAKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
LGANKWSIFTRVFLPNTAPGILSGCLLVLLPAMTLFYIPNVLGGARSILLGNLIQNQFLV
CCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]