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The map label for this gene is potD [H]

Identifier: 148358723

GI number: 148358723

Start: 1376418

End: 1377440

Strand: Reverse

Name: potD [H]

Synonym: LPC_0602

Alternate gene names: 148358723

Gene position: 1377440-1376418 (Counterclockwise)

Preceding gene: 148358724

Following gene: 148358722

Centisome position: 38.51

GC content: 38.81

Gene sequence:

>1023_bases
ATGAAAATTTTTTCATTACTGCTCGCTCTTCTTACATTTAAGGCATTCTCCAATCCAGTAGTCAATGTCTATGCCTGGGG
AGGTGAAATCCCTAAAAAAATAATTCATCAATTCGAACAAGAGTCAGGAATTAAAGTCAATTTTTCGACTTATGATAGCA
ATGAAACCATGTATGCCAAGCTAAGAGCCAGTCAAAAGAGCATTTATGATGTCATCCTCCCTTCCGCTTATTTTGTAGAG
CGCATGCAAAAACAAGGCATGTTGCAATTACTCGATCATAAACAGCTTTCGAATCTTCATAATCTGGATGAACAATTTGT
TAAAAATGATTATGATCCGGGTAACCGTTATAGTATACCCATCATCTGGGGAGCAACCGGGATTTTCTTTAATCATAATT
CCGTATCGAACCCGCCAAAAAGCTGGCAAGGCTTATGGGATAAAAAGTGGCGCAATCAGCTAATGTTACTTGATGATTCA
CGCGAAATATTTGCCATAGCTCTGATGAGTTTAGGGTTTGATCCCAATGACACCAATCCAAACCACATAAAAGCAGCCTA
TGAACACTTATTGCAATTGGTACCTAATATTAAGTTATTTGCCAGCGATAGTATTCAAGCCATCATGATAGATGAGGATG
CTTTTATTGGAACAGCATGGAATGGCGATGCCTTCAAAGCACATACAGAAAACAAAGCCATCGAGTTTATTTATCCTCAA
GATGGTTTTGTGATTTGGGTGGATTGTTTGGCTATCCCTAAAAACCCCCCACACCTGAAAGAGGCGTATCAATTCATTAA
TTTTATTCTGAAACCGGAAACAAGCGCTCAAATTGCACTAAAGGAAGGACATGCGATTACCAATGCCGCAGGAAGAGCTT
TATTGCCATTACCTATAAAAAATAACCCTGTGGTTTATCCGCCAGACAAAACACTAAAGAAGGGTTATTTCCAACGCGAC
ATCGGCGAAGAAACACTAGCGCTTTATAATCAATATTGGGAACAGTTAAAACTTGCTTTTTAG

Upstream 100 bases:

>100_bases
GTGCTGGTGTCACGCCTGAACTCAATGCATTGTGTTCAATTACGTTCGGTTTATCGATGATTCTCGTTATTATCTCTCAT
CGCTTGTCAGGTAAATTGAT

Downstream 100 bases:

>100_bases
TTTTCTGTTGTGAAGTAGCGCTAAATATCCAGATGAAGTACTTCATTAACTCAACGAATTAAAATTCAGAAAGCAAGGTT
TCTTGAATATTTTATATACA

Product: spermidine/putrescine-binding periplasmic protein PotD

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKIFSLLLALLTFKAFSNPVVNVYAWGGEIPKKIIHQFEQESGIKVNFSTYDSNETMYAKLRASQKSIYDVILPSAYFVE
RMQKQGMLQLLDHKQLSNLHNLDEQFVKNDYDPGNRYSIPIIWGATGIFFNHNSVSNPPKSWQGLWDKKWRNQLMLLDDS
REIFAIALMSLGFDPNDTNPNHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDAFIGTAWNGDAFKAHTENKAIEFIYPQ
DGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSAQIALKEGHAITNAAGRALLPLPIKNNPVVYPPDKTLKKGYFQRD
IGEETLALYNQYWEQLKLAF

Sequences:

>Translated_340_residues
MKIFSLLLALLTFKAFSNPVVNVYAWGGEIPKKIIHQFEQESGIKVNFSTYDSNETMYAKLRASQKSIYDVILPSAYFVE
RMQKQGMLQLLDHKQLSNLHNLDEQFVKNDYDPGNRYSIPIIWGATGIFFNHNSVSNPPKSWQGLWDKKWRNQLMLLDDS
REIFAIALMSLGFDPNDTNPNHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDAFIGTAWNGDAFKAHTENKAIEFIYPQ
DGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSAQIALKEGHAITNAAGRALLPLPIKNNPVVYPPDKTLKKGYFQRD
IGEETLALYNQYWEQLKLAF
>Mature_340_residues
MKIFSLLLALLTFKAFSNPVVNVYAWGGEIPKKIIHQFEQESGIKVNFSTYDSNETMYAKLRASQKSIYDVILPSAYFVE
RMQKQGMLQLLDHKQLSNLHNLDEQFVKNDYDPGNRYSIPIIWGATGIFFNHNSVSNPPKSWQGLWDKKWRNQLMLLDDS
REIFAIALMSLGFDPNDTNPNHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDAFIGTAWNGDAFKAHTENKAIEFIYPQ
DGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSAQIALKEGHAITNAAGRALLPLPIKNNPVVYPPDKTLKKGYFQRD
IGEETLALYNQYWEQLKLAF

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=326, Percent_Identity=40.1840490797546, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1787078, Length=309, Percent_Identity=32.0388349514563, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1787711, Length=310, Percent_Identity=22.258064516129, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38900; Mature: 38900

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFSLLLALLTFKAFSNPVVNVYAWGGEIPKKIIHQFEQESGIKVNFSTYDSNETMYAK
CCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEE
LRASQKSIYDVILPSAYFVERMQKQGMLQLLDHKQLSNLHNLDEQFVKNDYDPGNRYSIP
EHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
IIWGATGIFFNHNSVSNPPKSWQGLWDKKWRNQLMLLDDSREIFAIALMSLGFDPNDTNP
EEEECEEEEEECCCCCCCCHHHHCCHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCCCCC
NHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDAFIGTAWNGDAFKAHTENKAIEFIYPQ
HHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCEEEEECCCCCEEEECCCCEEEEEECC
DGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSAQIALKEGHAITNAAGRALLPLPIK
CCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCEEEEECCC
NNPVVYPPDKTLKKGYFQRDIGEETLALYNQYWEQLKLAF
CCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIFSLLLALLTFKAFSNPVVNVYAWGGEIPKKIIHQFEQESGIKVNFSTYDSNETMYAK
CCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEE
LRASQKSIYDVILPSAYFVERMQKQGMLQLLDHKQLSNLHNLDEQFVKNDYDPGNRYSIP
EHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
IIWGATGIFFNHNSVSNPPKSWQGLWDKKWRNQLMLLDDSREIFAIALMSLGFDPNDTNP
EEEECEEEEEECCCCCCCCHHHHCCHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCCCCC
NHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDAFIGTAWNGDAFKAHTENKAIEFIYPQ
HHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCEEEEECCCCCEEEECCCCEEEEEECC
DGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSAQIALKEGHAITNAAGRALLPLPIK
CCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCEEEEECCC
NNPVVYPPDKTLKKGYFQRDIGEETLALYNQYWEQLKLAF
CCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]