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The map label for this gene is fmt [H]

Identifier: 148358672

GI number: 148358672

Start: 3104039

End: 3104983

Strand: Reverse

Name: fmt [H]

Synonym: LPC_0548

Alternate gene names: 148358672

Gene position: 3104983-3104039 (Counterclockwise)

Preceding gene: 148358671

Following gene: 148358673

Centisome position: 86.82

GC content: 40.85

Gene sequence:

>945_bases
ATGAGTGGTTTAACTGTCGTTTTTGCTGGAACTCCAGAATTTGGCTTATCCTGTCTGGATGCTTTAATTCAATCAAGGCA
TCATCTCAAGGCTGTTTATACTCAGCCCGACAGGCCGGCTGGCCGAGGCCGTAAATTACAGGAGTCTCCTGTAAAAGAAT
GGGCAATAAATAACCAGGTACCAGTTTATCAACCTCTTAATTTTAAAAATCAAGAAGCTATCGATGAGCTTTCTGCCTTA
AAGCCTGATGTAATGGTCGTGATAGCCTATGGATTAATATTACCAAAAGCGGTGTTGGAAATTCCTCGTCTGGGTTGTAT
TAATGTACATGCTTCTCTGTTACCGCGCTGGCGTGGCGCATCTCCAATACAACATGCCATTTTACATGGTGATGCTGAAT
CCGGAGTGACCATTATGCAAATGGATGTTGGATTGGATACAGGTCCTATGTTATGCAAGGCCACCTGTCCTGTTACTTCC
TCGGATACCGCTGGTAGTTTGCATGACAAATTAGCAAAAATGTCAGTAAAACCATTATTGGATGTTTTGGAGGCTTTAGC
ATCCAATTCAGCACAATTTGAATTACAAAACAATGAATTAGCGACTTATGCTGGTAAAATTAATAAAGAAGAGGCACGAA
TTAACTGGCATCAGTCTGCAGTGGAAATAGATAGAAAAATTCGCGCATTTAATCCATGGCCTGTTGCCTATACGCTGGCT
GAGGAATTAATGCTCAGAATTCACCAGGCAAAAGCAACTGATATCATGAGTACCGAGATGCCGGGCATGGTATTAAATAT
CGATAAAAATGGAATGTTGGTCGCTACCAATGATAATGCCTTGTTAGTGGAAAAAATTCAATTTCCCGGAGCAAAGGTCA
TTTCAGTTAGGGATTGGTTAAATTCGGGTAAAACACAATTGCATACTGGTTTAATGTTGCAATGA

Upstream 100 bases:

>100_bases
ATATGAATGGCAAATTGTTTGTCGATATGTTGTCTCCATTGAAGAGAATGATGGCTCGCCGGAAATTAGATAAGTTCAAG
CGTTTACAAGCTCGTAAACC

Downstream 100 bases:

>100_bases
AAGTAAATGACAGATTCAACGCCTTAAAAATTTTGACGGCCTTGATAGAAAGCAGAAAAAACCTGTCCTATTTGATGTCT
CCGCAAGAGATTTCTCCCAT

Product: methionyl tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSGLTVVFAGTPEFGLSCLDALIQSRHHLKAVYTQPDRPAGRGRKLQESPVKEWAINNQVPVYQPLNFKNQEAIDELSAL
KPDVMVVIAYGLILPKAVLEIPRLGCINVHASLLPRWRGASPIQHAILHGDAESGVTIMQMDVGLDTGPMLCKATCPVTS
SDTAGSLHDKLAKMSVKPLLDVLEALASNSAQFELQNNELATYAGKINKEEARINWHQSAVEIDRKIRAFNPWPVAYTLA
EELMLRIHQAKATDIMSTEMPGMVLNIDKNGMLVATNDNALLVEKIQFPGAKVISVRDWLNSGKTQLHTGLMLQ

Sequences:

>Translated_314_residues
MSGLTVVFAGTPEFGLSCLDALIQSRHHLKAVYTQPDRPAGRGRKLQESPVKEWAINNQVPVYQPLNFKNQEAIDELSAL
KPDVMVVIAYGLILPKAVLEIPRLGCINVHASLLPRWRGASPIQHAILHGDAESGVTIMQMDVGLDTGPMLCKATCPVTS
SDTAGSLHDKLAKMSVKPLLDVLEALASNSAQFELQNNELATYAGKINKEEARINWHQSAVEIDRKIRAFNPWPVAYTLA
EELMLRIHQAKATDIMSTEMPGMVLNIDKNGMLVATNDNALLVEKIQFPGAKVISVRDWLNSGKTQLHTGLMLQ
>Mature_313_residues
SGLTVVFAGTPEFGLSCLDALIQSRHHLKAVYTQPDRPAGRGRKLQESPVKEWAINNQVPVYQPLNFKNQEAIDELSALK
PDVMVVIAYGLILPKAVLEIPRLGCINVHASLLPRWRGASPIQHAILHGDAESGVTIMQMDVGLDTGPMLCKATCPVTSS
DTAGSLHDKLAKMSVKPLLDVLEALASNSAQFELQNNELATYAGKINKEEARINWHQSAVEIDRKIRAFNPWPVAYTLAE
ELMLRIHQAKATDIMSTEMPGMVLNIDKNGMLVATNDNALLVEKIQFPGAKVISVRDWLNSGKTQLHTGLMLQ

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI238814322, Length=316, Percent_Identity=27.8481012658228, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI21614513, Length=322, Percent_Identity=28.8819875776398, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI164663775, Length=236, Percent_Identity=36.0169491525424, Blast_Score=118, Evalue=7e-27,
Organism=Escherichia coli, GI1789683, Length=307, Percent_Identity=52.1172638436482, Blast_Score=328, Evalue=2e-91,
Organism=Escherichia coli, GI1788589, Length=278, Percent_Identity=27.3381294964029, Blast_Score=120, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI133930964, Length=304, Percent_Identity=25, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6319458, Length=351, Percent_Identity=25.9259259259259, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45550868, Length=309, Percent_Identity=28.4789644012945, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28571984, Length=228, Percent_Identity=29.8245614035088, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585660, Length=329, Percent_Identity=25.8358662613982, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 34456; Mature: 34325

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLTVVFAGTPEFGLSCLDALIQSRHHLKAVYTQPDRPAGRGRKLQESPVKEWAINNQV
CCCEEEEEECCCHHHHHHHHHHHHCCHHEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCC
PVYQPLNFKNQEAIDELSALKPDVMVVIAYGLILPKAVLEIPRLGCINVHASLLPRWRGA
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHCCCCCCC
SPIQHAILHGDAESGVTIMQMDVGLDTGPMLCKATCPVTSSDTAGSLHDKLAKMSVKPLL
CHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
DVLEALASNSAQFELQNNELATYAGKINKEEARINWHQSAVEIDRKIRAFNPWPVAYTLA
HHHHHHHCCCEEEEECCCCEEEHHCCCCCCHHHEEHHHHHHHHHHHHCCCCCCCHHHHHH
EELMLRIHQAKATDIMSTEMPGMVLNIDKNGMLVATNDNALLVEKIQFPGAKVISVRDWL
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCEEEEEEECCCCCEEEEEHHHH
NSGKTQLHTGLMLQ
CCCCCEECCCCCCC
>Mature Secondary Structure 
SGLTVVFAGTPEFGLSCLDALIQSRHHLKAVYTQPDRPAGRGRKLQESPVKEWAINNQV
CCEEEEEECCCHHHHHHHHHHHHCCHHEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCC
PVYQPLNFKNQEAIDELSALKPDVMVVIAYGLILPKAVLEIPRLGCINVHASLLPRWRGA
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHCCCCCCC
SPIQHAILHGDAESGVTIMQMDVGLDTGPMLCKATCPVTSSDTAGSLHDKLAKMSVKPLL
CHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
DVLEALASNSAQFELQNNELATYAGKINKEEARINWHQSAVEIDRKIRAFNPWPVAYTLA
HHHHHHHCCCEEEEECCCCEEEHHCCCCCCHHHEEHHHHHHHHHHHHCCCCCCCHHHHHH
EELMLRIHQAKATDIMSTEMPGMVLNIDKNGMLVATNDNALLVEKIQFPGAKVISVRDWL
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCEEEEEEECCCCCEEEEEHHHH
NSGKTQLHTGLMLQ
CCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA