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The map label for this gene is rpoH [H]

Identifier: 148358598

GI number: 148358598

Start: 3190808

End: 3191662

Strand: Reverse

Name: rpoH [H]

Synonym: LPC_0471

Alternate gene names: 148358598

Gene position: 3191662-3190808 (Counterclockwise)

Preceding gene: 148358597

Following gene: 148358600

Centisome position: 89.24

GC content: 40.7

Gene sequence:

>855_bases
ATGAGTCAACAGTTGCAACTTGCTGCAATGAATCTACCTGTTGGTAGTCTTGATTCTTATATCCATCGAGTAAATCAAAT
TCCAATGCTGACTTTGGAAGAGGAAATTGCATATGCCGAGCGTTTTCATTCTGAAGGAGATATAGAAGCTGCTCGACAAT
TAGTTCTTGCGCATTTGCGCTATGTAGTTCGTGTAGCCCGCGGTTATCTGGGGTATGGATTGCCTTTAAGTGATTTAATT
CAGGAAGGTAATGTAGGCTTGATGAAAGCCGTAAAACGTTTTGACCCCAAGATGGGCGTACGCCTTGTTTCTTTTGCTGT
TCATTGGATTAAAGCAGAAATTCATGAGTTTGTGCTGCGTAACTGGCGAATAGTTAAAGTTGCTACAACCAAGGCCCAAC
GTAAATTATTTTTTAATTTGCGTCAAATGAAAAATCGATTGGGTTGGTTCAGCAATGAAGAAGTTGACGCTGTCGCCAAA
GATTTGGGCGTTAGCCGAGAAGACGTGTTGTTGATGGAGCAACGTTTAAATGTTATGGATTCATCTTATGATGCGCCAGA
TGTTGATGATAATGATGATGCTTACAAAGCGCCTGAGCGTTATTTGTTTAATGTGAACGATGATCCTGCTGTCTTGTTAG
AGAATGAGGATACTGGCGATCAGGGGCGTGAAAAATTACTGTTTGCTATGGAACAACTGGATGAACGTAGCCAGGATATT
TTACAACAACGCTGGCTTGCTGAAGAAAAACTCACGTTACATGATTTGGCAGAAAAATATGGTGTCTCTGCTGAACGGGT
TAGGCAACTTGAAAAAAATGCCATGAAAAAAATCCGTCAATACATGGAAAGTTAA

Upstream 100 bases:

>100_bases
TTTGAAAATTTATAGCTTAGCATTCATTTTGGTGAATCCTATGAGTTAATAGGATATAAATTTTAATACTATTCTAGTGT
TAATGACCGGAGGAAGTAGT

Downstream 100 bases:

>100_bases
TTACAAAATTTTGAAGCCAACTCCTCTGTATGTGAAACTCGTTATTTCAGCAAACACAATGGTCTGCCTAGCTACTGGAT
ACATCTGGATTAGGTAAAAC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSQQLQLAAMNLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHSEGDIEAARQLVLAHLRYVVRVARGYLGYGLPLSDLI
QEGNVGLMKAVKRFDPKMGVRLVSFAVHWIKAEIHEFVLRNWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAK
DLGVSREDVLLMEQRLNVMDSSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDTGDQGREKLLFAMEQLDERSQDI
LQQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMKKIRQYMES

Sequences:

>Translated_284_residues
MSQQLQLAAMNLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHSEGDIEAARQLVLAHLRYVVRVARGYLGYGLPLSDLI
QEGNVGLMKAVKRFDPKMGVRLVSFAVHWIKAEIHEFVLRNWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAK
DLGVSREDVLLMEQRLNVMDSSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDTGDQGREKLLFAMEQLDERSQDI
LQQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMKKIRQYMES
>Mature_283_residues
SQQLQLAAMNLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHSEGDIEAARQLVLAHLRYVVRVARGYLGYGLPLSDLIQ
EGNVGLMKAVKRFDPKMGVRLVSFAVHWIKAEIHEFVLRNWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAKD
LGVSREDVLLMEQRLNVMDSSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDTGDQGREKLLFAMEQLDERSQDIL
QQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMKKIRQYMES

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=285, Percent_Identity=59.2982456140351, Blast_Score=327, Evalue=4e-91,
Organism=Escherichia coli, GI1789098, Length=269, Percent_Identity=28.996282527881, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789448, Length=244, Percent_Identity=27.0491803278689, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32887; Mature: 32756

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQLQLAAMNLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHSEGDIEAARQLVLAHLR
CCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
YVVRVARGYLGYGLPLSDLIQEGNVGLMKAVKRFDPKMGVRLVSFAVHWIKAEIHEFVLR
HHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
NWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAKDLGVSREDVLLMEQRLNVMD
CCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
SSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDTGDQGREKLLFAMEQLDERSQDI
CCCCCCCCCCCCCHHCCCHHEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LQQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMKKIRQYMES
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQQLQLAAMNLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHSEGDIEAARQLVLAHLR
CCCHHHHHHCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
YVVRVARGYLGYGLPLSDLIQEGNVGLMKAVKRFDPKMGVRLVSFAVHWIKAEIHEFVLR
HHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
NWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAKDLGVSREDVLLMEQRLNVMD
CCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
SSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDTGDQGREKLLFAMEQLDERSQDI
CCCCCCCCCCCCCHHCCCHHEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LQQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMKKIRQYMES
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]