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The map label for this gene is rsmD [H]

Identifier: 148358592

GI number: 148358592

Start: 3197389

End: 3197814

Strand: Direct

Name: rsmD [H]

Synonym: LPC_0464

Alternate gene names: 148358592

Gene position: 3197389-3197814 (Clockwise)

Preceding gene: 148358593

Following gene: 148358591

Centisome position: 89.4

GC content: 36.85

Gene sequence:

>426_bases
ATGAATGATATTAAAAATGCCCGCTGTCTGGATGCTTTTGCAGGTAGTGGCGCGTTGGGATTTGAAGCCTATTCCAGAGG
AGCATCTCATGTCGTTTTTATCGAACAATCTCCCAAAGCTCATGCCAGTTTGCAAAATACCATTCGTCAATTTGATACCC
CCAAATTGCACCTTATAAAAACTGATACGTTAAATTATCTTAAACAAAGCACCGAGCAATTCGATCTGATTTTTTTAGAT
CCTCCCTACGCTCTCAACTATGTTCCTCAGTGTCTTGATCATATCATAACAAATAACCTTCTTGTTAAAGGAGGTCTTGT
CTACATTGAATCATCATCTCCTATTGAAGTAAAAACAGAACTTTGGAAGCAATTGAAATTAAAACAAGCCGGTCAAGTCA
TTTATGGATTATTTGAAAAACTCTAA

Upstream 100 bases:

>100_bases
TATTGGAGGATTATATCGGGGGAAAAAAATACATTTCCCCGATGTAGATGGCCTGAGGCCAACACCAGACAGAGTTCGGG
AGACCCTCTTTAATTGGTTA

Downstream 100 bases:

>100_bases
CAAACAATGTCTTGACAACAAGCCTTCCTCCTGTTTCAATCAAACTAATATTTCCAAATGGTAAAGAACATTGAATGAAC
AACAATAAGATTGTCATAAT

Product: N6-adenine specific methylase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 141; Mature: 141

Protein sequence:

>141_residues
MNDIKNARCLDAFAGSGALGFEAYSRGASHVVFIEQSPKAHASLQNTIRQFDTPKLHLIKTDTLNYLKQSTEQFDLIFLD
PPYALNYVPQCLDHIITNNLLVKGGLVYIESSSPIEVKTELWKQLKLKQAGQVIYGLFEKL

Sequences:

>Translated_141_residues
MNDIKNARCLDAFAGSGALGFEAYSRGASHVVFIEQSPKAHASLQNTIRQFDTPKLHLIKTDTLNYLKQSTEQFDLIFLD
PPYALNYVPQCLDHIITNNLLVKGGLVYIESSSPIEVKTELWKQLKLKQAGQVIYGLFEKL
>Mature_141_residues
MNDIKNARCLDAFAGSGALGFEAYSRGASHVVFIEQSPKAHASLQNTIRQFDTPKLHLIKTDTLNYLKQSTEQFDLIFLD
PPYALNYVPQCLDHIITNNLLVKGGLVYIESSSPIEVKTELWKQLKLKQAGQVIYGLFEKL

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=143, Percent_Identity=30.7692307692308, Blast_Score=78, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 15856; Mature: 15856

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIKNARCLDAFAGSGALGFEAYSRGASHVVFIEQSPKAHASLQNTIRQFDTPKLHLIK
CCCCCCCHHHHHHCCCCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEE
TDTLNYLKQSTEQFDLIFLDPPYALNYVPQCLDHIITNNLLVKGGLVYIESSSPIEVKTE
HHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCEEEECCEEEEECCCCEEHHHH
LWKQLKLKQAGQVIYGLFEKL
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNDIKNARCLDAFAGSGALGFEAYSRGASHVVFIEQSPKAHASLQNTIRQFDTPKLHLIK
CCCCCCCHHHHHHCCCCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEE
TDTLNYLKQSTEQFDLIFLDPPYALNYVPQCLDHIITNNLLVKGGLVYIESSSPIEVKTE
HHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCEEEECCEEEEECCCCEEHHHH
LWKQLKLKQAGQVIYGLFEKL
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]