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The map label for this gene is amiB [H]

Identifier: 148358565

GI number: 148358565

Start: 3227757

End: 3229187

Strand: Reverse

Name: amiB [H]

Synonym: LPC_0437

Alternate gene names: 148358565

Gene position: 3229187-3227757 (Counterclockwise)

Preceding gene: 148358564

Following gene: 148358566

Centisome position: 90.29

GC content: 41.72

Gene sequence:

>1431_bases
ATGATGATGCGAGCGTGGGTCTTAAGTCTGTTATTATATCTACCAAGTTCTTTTGCTGTATTTTCTGCACAATTAAAGAA
TATTACAGTTTCTCAGCAACCAACCATGACGTCTCTTTATCTTTCTATACAAGGTTCATTTACCCATAAGCTATTTACCT
TAAGTCAGCCCAATCGCGTGGTTTTAGACCTAAGTGATACACAATTAGCCGTCAATATCAGTCAATTGGGTTTAATCAAT
TCGCTTATTCAGCAGGTAAGAAGCGGTAGTCCCTCCAAAAACACGCTCAGGCTTGTCATGGATGTAAAACAACCAGTCAA
GGTGCGCTCATCTTCATGGAAACCGAAAGGAGAATTTAATGGGATCAGAATTGATCTTGTTAATGATACTCCTTTAGTCA
TAAATAATCGCAGGCAGTTGAGCTCGCCTGTTAAAACGATTAAAGCTCCTGTCCAGCAAATCCAATCAAAAATATCCAGT
CAGCCATCAGAACAACGAATAACTGTGCATAAAACACCTGTCCAGGTTCCTCATAACCCATCAAAAAAACCTTTGCGTGA
TGTTGTTGTAGTGTTGGATGCAGGGCATGGGGGCAAAGATCCTGGTGCAAGAGGTCCGCGCCGCAGTAACGAGAAGGATG
TTGTGCTGGCCATTACTTTAAAACTTAAACGATTGATAGACAGGCAACCAGGTATGCGCGCAGTCCTGACACGATCGGGA
GATTATTACGTTGGACTTAGACAACGCCTGGATATTGCCAGAAAATATAATGGTGATGTATTCATTTCTATCCATGCCGA
TGCATTCAATAATCCTCACTCTAATGGAGCCTCGGTATTTGCCTTGTCACAAAGGGGGGCCACCAGTGAAGCAGCACGCT
GGTTAGCAGAAAAGGAGAATTACTCTGAACTAGGTGGAGTGAATTTAGGTGAGTTGGATGATAGTAATGGGGTAGTACGC
TCTGTTTTGATCGATTTATCACAAACTGCGACTATAAATGCTGGTTTGGAAATGGGGGGAAAAGTATTAGGTCAATTGGA
TAATTTTACCAATTTGCATAATAACAAAGTGGAGCAAGCCCGTTTTGTTGTTCTGAAATCTCCAGATATTCCTTCTATTT
TGGTTGAAACCGGTTTTATCTCAAATCCCCGTGAGGAAAGAAATTTAACCAACCCTGCCTATCAGGAGCGATTGAGTCAG
GCAATATTTCAAGGACTAAAAAATTATTTTTGGGATCATCCTCCTCATGGGACTCGAATTGAGGCTATGATTTCTCCAAA
TTTTCATATCGTTAAAAAGGGTGAGACTCTTCCTGCTATCGCTGCTCGATATAGGGTATCTGTGAGCGCATTACAATCAA
TTAACCGTCTTCAGGCAAGGGCAAGGCTAATGCCCGGGCAAAAATTAGTGATACCTGGCACATGGTCTTAA

Upstream 100 bases:

>100_bases
TTCAGTTTAATTTAATAATTAAAGGGACTGGGCGCTTAATACAAATAGCTGCATTAAGTGTCGCGGGTAAAAGAATTTTA
TCTTGCCTGGCGGGTGAATG

Downstream 100 bases:

>100_bases
GGATACATCAACTTTCGCCAGCAATAGCCAATCAGATTGCAGCAGGAGAAGTTATAGAAAGACCTGCTTCGGTAGTCAAG
GAGCTGCTGGAAAACTCTTT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQGSFTHKLFTLSQPNRVVLDLSDTQLAVNISQLGLIN
SLIQQVRSGSPSKNTLRLVMDVKQPVKVRSSSWKPKGEFNGIRIDLVNDTPLVINNRRQLSSPVKTIKAPVQQIQSKISS
QPSEQRITVHKTPVQVPHNPSKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAITLKLKRLIDRQPGMRAVLTRSG
DYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNGASVFALSQRGATSEAARWLAEKENYSELGGVNLGELDDSNGVVR
SVLIDLSQTATINAGLEMGGKVLGQLDNFTNLHNNKVEQARFVVLKSPDIPSILVETGFISNPREERNLTNPAYQERLSQ
AIFQGLKNYFWDHPPHGTRIEAMISPNFHIVKKGETLPAIAARYRVSVSALQSINRLQARARLMPGQKLVIPGTWS

Sequences:

>Translated_476_residues
MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQGSFTHKLFTLSQPNRVVLDLSDTQLAVNISQLGLIN
SLIQQVRSGSPSKNTLRLVMDVKQPVKVRSSSWKPKGEFNGIRIDLVNDTPLVINNRRQLSSPVKTIKAPVQQIQSKISS
QPSEQRITVHKTPVQVPHNPSKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAITLKLKRLIDRQPGMRAVLTRSG
DYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNGASVFALSQRGATSEAARWLAEKENYSELGGVNLGELDDSNGVVR
SVLIDLSQTATINAGLEMGGKVLGQLDNFTNLHNNKVEQARFVVLKSPDIPSILVETGFISNPREERNLTNPAYQERLSQ
AIFQGLKNYFWDHPPHGTRIEAMISPNFHIVKKGETLPAIAARYRVSVSALQSINRLQARARLMPGQKLVIPGTWS
>Mature_476_residues
MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQGSFTHKLFTLSQPNRVVLDLSDTQLAVNISQLGLIN
SLIQQVRSGSPSKNTLRLVMDVKQPVKVRSSSWKPKGEFNGIRIDLVNDTPLVINNRRQLSSPVKTIKAPVQQIQSKISS
QPSEQRITVHKTPVQVPHNPSKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAITLKLKRLIDRQPGMRAVLTRSG
DYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNGASVFALSQRGATSEAARWLAEKENYSELGGVNLGELDDSNGVVR
SVLIDLSQTATINAGLEMGGKVLGQLDNFTNLHNNKVEQARFVVLKSPDIPSILVETGFISNPREERNLTNPAYQERLSQ
AIFQGLKNYFWDHPPHGTRIEAMISPNFHIVKKGETLPAIAARYRVSVSALQSINRLQARARLMPGQKLVIPGTWS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=370, Percent_Identity=37.5675675675676, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1790611, Length=431, Percent_Identity=34.8027842227378, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1788776, Length=225, Percent_Identity=48.4444444444444, Blast_Score=204, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 52733; Mature: 52733

Theoretical pI: Translated: 10.84; Mature: 10.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQGSFTHKLFTLSQPNRV
CCHHHHHHHHHHHCCCCHHHHHHHHHCEEECCCCCEEEEEEEEECCEEEEEEEECCCCEE
VLDLSDTQLAVNISQLGLINSLIQQVRSGSPSKNTLRLVMDVKQPVKVRSSSWKPKGEFN
EEEECCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHCCCCCCCCCCC
GIRIDLVNDTPLVINNRRQLSSPVKTIKAPVQQIQSKISSQPSEQRITVHKTPVQVPHNP
CEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEECCCCC
SKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAITLKLKRLIDRQPGMRAVLTRSG
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEECCC
DYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNGASVFALSQRGATSEAARWLAEKEN
CEEEEHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCC
YSELGGVNLGELDDSNGVVRSVLIDLSQTATINAGLEMGGKVLGQLDNFTNLHNNKVEQA
HHHHCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCE
RFVVLKSPDIPSILVETGFISNPREERNLTNPAYQERLSQAIFQGLKNYFWDHPPHGTRI
EEEEEECCCCCEEEEECCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
EAMISPNFHIVKKGETLPAIAARYRVSVSALQSINRLQARARLMPGQKLVIPGTWS
EEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
>Mature Secondary Structure
MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQGSFTHKLFTLSQPNRV
CCHHHHHHHHHHHCCCCHHHHHHHHHCEEECCCCCEEEEEEEEECCEEEEEEEECCCCEE
VLDLSDTQLAVNISQLGLINSLIQQVRSGSPSKNTLRLVMDVKQPVKVRSSSWKPKGEFN
EEEECCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHCCCCCCCCCCC
GIRIDLVNDTPLVINNRRQLSSPVKTIKAPVQQIQSKISSQPSEQRITVHKTPVQVPHNP
CEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEECCCCC
SKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAITLKLKRLIDRQPGMRAVLTRSG
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEECCC
DYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNGASVFALSQRGATSEAARWLAEKEN
CEEEEHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCC
YSELGGVNLGELDDSNGVVRSVLIDLSQTATINAGLEMGGKVLGQLDNFTNLHNNKVEQA
HHHHCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCE
RFVVLKSPDIPSILVETGFISNPREERNLTNPAYQERLSQAIFQGLKNYFWDHPPHGTRI
EEEEEECCCCCEEEEECCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
EAMISPNFHIVKKGETLPAIAARYRVSVSALQSINRLQARARLMPGQKLVIPGTWS
EEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]