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The map label for this gene is tgt [H]

Identifier: 148358534

GI number: 148358534

Start: 3255349

End: 3256500

Strand: Reverse

Name: tgt [H]

Synonym: LPC_0405

Alternate gene names: 148358534

Gene position: 3256500-3255349 (Counterclockwise)

Preceding gene: 148358533

Following gene: 148358535

Centisome position: 91.05

GC content: 40.8

Gene sequence:

>1152_bases
ATGAACACTTTTTCATGTGAAGTACTGCACCAGCATTCGGGTACCAAAGCTCGTGTTACACGTGTGGTTACACCTCATGG
TGAATTTATAACCCCGGTATTTATGCCAGTTGGAACCCGTGCAGGGGTCAATAACATGATGCCGTCAGAACTGTCTGCTG
CGGGTACTCAAATTATTCTGGGCGGCAATACCTATCATATGTTGTGTGCGCCTGGCATGAAGGTGATAGAAAATGCAGGG
GGGATGCATCGCTTTATGGGGTGGCATGGGCCTATGCTAACAGACAGCGGTGGTTTTCAGGTTTTTAGTTTATCGAAGAA
CAAGGAAATTTGTTCTATAGATGAATCTGGTGCTCATTTTAAATTGCCTGGCAGTAAGGGGATTATCCATATGACTCCGG
AAATGTCTCTGGAGACCCAGAAAATAATTGGTGCCGATATTATAATGGCATTTGATCAGTGTACTCCTGATAGCTGCTCC
AAAGATGAAGTAAGTCAAATCATGCTGCGGACCCACAGATGGCTAAAACAATCGATTGACTACCATCAACAATATCCCAA
CTCTCGATATGGATATAAACAAGCTTTGTTTGGCATTGTGCAAGGGGGAGTTTTTCAAGATTTACGTCGAGAAAGTGCTG
ATTTTGTTTCTGCAATGAATACGGATGGAATTGCAATAGGTGGAGAAACCGTAGGGTTTGATATGAATACTACGGTAGAA
GTCATTCGTTGGGTGCATGAGTATCTTCCGGACAATAAGCCACGTTATACTATGGGGGTTGGAATGTCACCTCAAGATCT
GTTGGATGTGGTGGCAGAAGGTATCGATATGTTTGATTGTGTTGCGCCAACACGTAATGCCCGGCATGGTGCCTTATATT
GTGGTAAATTGGTAAGAGAAGGGAATTGGCTAAAATTTCAAAGCGAACATGAAAATGAACGAATTCAAATAAAGAAATCA
TGTTTTGCTGATGATTTGGCACCTGTTATGGAGAGTTGTTCTTGTTATACTTGTCTTAATTATTCAAGATCCTATTTGCA
TCATTTATCAAAACAAAAAGCTAATTTATTTACTGCTTTGGCAAGCATCCATAATGTTCATGTTTTGCATGATGTTTGTA
ATCACATGCGTCATTTGATAATAAATTCCTAA

Upstream 100 bases:

>100_bases
GGGCTTTTTCTGTTTTTTCGAACCCGAACGATCAATTAATTCCTGGCATTAAACGCGTGCTTTCGTTATCATTCTTTGTT
TTATCTTGTCGTTTCTGTGA

Downstream 100 bases:

>100_bases
ATTATTTAATATTAAGAGGTGAAATAATGGTTTTAATTTCTACATCCATGGGTGATATTCATATCAAATTAGATACAGAA
AATACCCCATTAACTGCAGA

Product: queuine/archaeosine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MNTFSCEVLHQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAGTQIILGGNTYHMLCAPGMKVIENAG
GMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDESGAHFKLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCS
KDEVSQIMLRTHRWLKQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDMNTTVE
VIRWVHEYLPDNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCGKLVREGNWLKFQSEHENERIQIKKS
CFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKANLFTALASIHNVHVLHDVCNHMRHLIINS

Sequences:

>Translated_383_residues
MNTFSCEVLHQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAGTQIILGGNTYHMLCAPGMKVIENAG
GMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDESGAHFKLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCS
KDEVSQIMLRTHRWLKQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDMNTTVE
VIRWVHEYLPDNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCGKLVREGNWLKFQSEHENERIQIKKS
CFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKANLFTALASIHNVHVLHDVCNHMRHLIINS
>Mature_383_residues
MNTFSCEVLHQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAGTQIILGGNTYHMLCAPGMKVIENAG
GMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDESGAHFKLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCS
KDEVSQIMLRTHRWLKQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDMNTTVE
VIRWVHEYLPDNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCGKLVREGNWLKFQSEHENERIQIKKS
CFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKANLFTALASIHNVHVLHDVCNHMRHLIINS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=376, Percent_Identity=36.1702127659575, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI13375872, Length=276, Percent_Identity=23.9130434782609, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786607, Length=365, Percent_Identity=41.6438356164384, Blast_Score=272, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17544680, Length=364, Percent_Identity=36.2637362637363, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI25146502, Length=285, Percent_Identity=25.2631578947368, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920510, Length=374, Percent_Identity=37.9679144385027, Blast_Score=240, Evalue=9e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42739; Mature: 42739

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFSCEVLHQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAGTQIIL
CCCEEEEHHCCCCCCCEEEEEEECCCCCEECHHHCCCCCCCCCCCCCCHHHHHCCCEEEE
GGNTYHMLCAPGMKVIENAGGMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDESGAHF
CCCEEEEEECCCHHHHHCCCCHHHHHCCCCCEEECCCCEEEEEECCCCCEEEECCCCCEE
KLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCSKDEVSQIMLRTHRWLKQSID
ECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDMNTTVE
HHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCCHHHH
VIRWVHEYLPDNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCGKLVRE
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHC
GNWLKFQSEHENERIQIKKSCFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKANLFTAL
CCEEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ASIHNVHVLHDVCNHMRHLIINS
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTFSCEVLHQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVNNMMPSELSAAGTQIIL
CCCEEEEHHCCCCCCCEEEEEEECCCCCEECHHHCCCCCCCCCCCCCCHHHHHCCCEEEE
GGNTYHMLCAPGMKVIENAGGMHRFMGWHGPMLTDSGGFQVFSLSKNKEICSIDESGAHF
CCCEEEEEECCCHHHHHCCCCHHHHHCCCCCEEECCCCEEEEEECCCCCEEEECCCCCEE
KLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCSKDEVSQIMLRTHRWLKQSID
ECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSAMNTDGIAIGGETVGFDMNTTVE
HHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCCHHHH
VIRWVHEYLPDNKPRYTMGVGMSPQDLLDVVAEGIDMFDCVAPTRNARHGALYCGKLVRE
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHC
GNWLKFQSEHENERIQIKKSCFADDLAPVMESCSCYTCLNYSRSYLHHLSKQKANLFTAL
CCEEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ASIHNVHVLHDVCNHMRHLIINS
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]