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The map label for this gene is oprM [H]

Identifier: 148358466

GI number: 148358466

Start: 355564

End: 356910

Strand: Direct

Name: oprM [H]

Synonym: LPC_0332

Alternate gene names: 148358466

Gene position: 355564-356910 (Clockwise)

Preceding gene: 148358465

Following gene: 148358467

Centisome position: 9.94

GC content: 41.43

Gene sequence:

>1347_bases
TTGTATAGGAAGCGATGGCTCATTTTATTGTGTGCTTTGTTGAATAATGGTTGCCTCTGGCTTGGGTCAGAATACAGGAA
ACCTCATGTCAACACCCCGCAAAAATGGCCGCATAAATATTCAATTCAAACTGAGCAAGCTGCCTATTTACCTGATCTGC
ATTGGTGGAAACAGTTCAATAGCAAGGAGCTCAATGCTTCTATCCAAAAGGCATTACAAAATAATCACCAGATTCACCTC
GCTATGGCAAATATTGAATCCGCACAGGGGCAATTGCGGCAAGTCCAGTTAAGTTGGTTGCCTAATCTCACAGGGCTTGC
AGGCTACACCCAGTTTCCCGTTTTAGGTAATCCGGGGACTACGGCAATTGCCTATCCAGCCTACATCATTAATATTTTCC
AGCAATATAAGCAACAAAAAAGCGCGCAAGCCATGCTTGAAGCCAGTATTTACGCGCAATATTCTGCCAGACTGGTCGTG
ATAGCCCAGACTGCAGCCAGCTTTTTTACCTTGGTTGCTCAGAATGAAGCTTTGCATTTATACAATAAACTGCTTAAGGA
TTATCGAACCTATCTCAAACTGACTCAAAGCCAATATCGCTCAGGATTGATTTCGCTTGATAATATCACTCAAATCAAAA
GTCATATCCAACGAATTGAGGCACAGATCAAGGTGGTTCAGCATAACATTGTGGTGACTAAAAACGCCTTGCATTTTTTA
TTTAATGAAAATCCTGGAGATGTCGAGACTAAAACTCTCTTTGAAAATATCGATAGTAATCAATTGGTACCAGGGAATTT
ACCTGCCAGCGTTCTCAGAGTGAGACCTGATATTCATGAGGCAGAAGCGTTGTTAAAAGCAGCGCATGCCGATGTGGGTG
CGATAGCCGCGAATCTTTTACCAGGGATTAATTTGGGCGCTTACCTGGGTGAGGGATCTAATGTGGATGGCGCCATTAAA
TTAGGGCAAGCTTATTTAAACGGCCCGATAATTGATTTGCCTCTATTTGCTCAGGTTGATGTGAGTAAAGCTCGATATAA
GGCAATCTATATCAAATACATTACTACTATTCGTAAGGCCTTGCGTGATGTTGCTAATGACTTGTCTGCCTATTCAACTT
CCAGCCAGCAATTAAATAATAATTTATCGGCTTTAAGCGATGAAAAACAGCAATGCCATTTGGCAGAAGTTCGTTATCGT
CACGGAATTGATGATTATTTAAATCTAATAAAATGCCAAATCCTGTTAGACGAATTTAAGTTGGTGATAAACCAGAACAA
GCTGGAAAAACTGCTATCCATAGTCGCTTTATACCAGGATTTAGGAGGTGGATACCGTGGGCATTGA

Upstream 100 bases:

>100_bases
ACTATTGCATTCCATTAGCTTGGTTTGCGTAATACTCTCTTGACAACTACAAGATTGAAAGCTCAATATTCTTTATTTAC
ACCAAGGTTCAGGATTTTCA

Downstream 100 bases:

>100_bases
TCAGCAAAAGACCGTCCGTACCTTGCGCTTATCCCTGACGTGTATTTTTCTTTTTCTCATTACCTGGTACTACCAAGTCC
CAGAAAGCGCATGGACTTTA

Product: outer membrane channel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MYRKRWLILLCALLNNGCLWLGSEYRKPHVNTPQKWPHKYSIQTEQAAYLPDLHWWKQFNSKELNASIQKALQNNHQIHL
AMANIESAQGQLRQVQLSWLPNLTGLAGYTQFPVLGNPGTTAIAYPAYIINIFQQYKQQKSAQAMLEASIYAQYSARLVV
IAQTAASFFTLVAQNEALHLYNKLLKDYRTYLKLTQSQYRSGLISLDNITQIKSHIQRIEAQIKVVQHNIVVTKNALHFL
FNENPGDVETKTLFENIDSNQLVPGNLPASVLRVRPDIHEAEALLKAAHADVGAIAANLLPGINLGAYLGEGSNVDGAIK
LGQAYLNGPIIDLPLFAQVDVSKARYKAIYIKYITTIRKALRDVANDLSAYSTSSQQLNNNLSALSDEKQQCHLAEVRYR
HGIDDYLNLIKCQILLDEFKLVINQNKLEKLLSIVALYQDLGGGYRGH

Sequences:

>Translated_448_residues
MYRKRWLILLCALLNNGCLWLGSEYRKPHVNTPQKWPHKYSIQTEQAAYLPDLHWWKQFNSKELNASIQKALQNNHQIHL
AMANIESAQGQLRQVQLSWLPNLTGLAGYTQFPVLGNPGTTAIAYPAYIINIFQQYKQQKSAQAMLEASIYAQYSARLVV
IAQTAASFFTLVAQNEALHLYNKLLKDYRTYLKLTQSQYRSGLISLDNITQIKSHIQRIEAQIKVVQHNIVVTKNALHFL
FNENPGDVETKTLFENIDSNQLVPGNLPASVLRVRPDIHEAEALLKAAHADVGAIAANLLPGINLGAYLGEGSNVDGAIK
LGQAYLNGPIIDLPLFAQVDVSKARYKAIYIKYITTIRKALRDVANDLSAYSTSSQQLNNNLSALSDEKQQCHLAEVRYR
HGIDDYLNLIKCQILLDEFKLVINQNKLEKLLSIVALYQDLGGGYRGH
>Mature_448_residues
MYRKRWLILLCALLNNGCLWLGSEYRKPHVNTPQKWPHKYSIQTEQAAYLPDLHWWKQFNSKELNASIQKALQNNHQIHL
AMANIESAQGQLRQVQLSWLPNLTGLAGYTQFPVLGNPGTTAIAYPAYIINIFQQYKQQKSAQAMLEASIYAQYSARLVV
IAQTAASFFTLVAQNEALHLYNKLLKDYRTYLKLTQSQYRSGLISLDNITQIKSHIQRIEAQIKVVQHNIVVTKNALHFL
FNENPGDVETKTLFENIDSNQLVPGNLPASVLRVRPDIHEAEALLKAAHADVGAIAANLLPGINLGAYLGEGSNVDGAIK
LGQAYLNGPIIDLPLFAQVDVSKARYKAIYIKYITTIRKALRDVANDLSAYSTSSQQLNNNLSALSDEKQQCHLAEVRYR
HGIDDYLNLIKCQILLDEFKLVINQNKLEKLLSIVALYQDLGGGYRGH

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=464, Percent_Identity=24.7844827586207, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI2367348, Length=479, Percent_Identity=22.1294363256785, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50420; Mature: 50420

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRKRWLILLCALLNNGCLWLGSEYRKPHVNTPQKWPHKYSIQTEQAAYLPDLHWWKQFN
CCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHCC
SKELNASIQKALQNNHQIHLAMANIESAQGQLRQVQLSWLPNLTGLAGYTQFPVLGNPGT
CHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHEEHHHHCCCCCCCCCCCCCCEECCCCC
TAIAYPAYIINIFQQYKQQKSAQAMLEASIYAQYSARLVVIAQTAASFFTLVAQNEALHL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHCCCHHHH
YNKLLKDYRTYLKLTQSQYRSGLISLDNITQIKSHIQRIEAQIKVVQHNIVVTKNALHFL
HHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCEEEE
FNENPGDVETKTLFENIDSNQLVPGNLPASVLRVRPDIHEAEALLKAAHADVGAIAANLL
ECCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHC
PGINLGAYLGEGSNVDGAIKLGQAYLNGPIIDLPLFAQVDVSKARYKAIYIKYITTIRKA
CCCCCHHEECCCCCCCHHHHHHHHHHCCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHH
LRDVANDLSAYSTSSQQLNNNLSALSDEKQQCHLAEVRYRHGIDDYLNLIKCQILLDEFK
HHHHHHHHHHHCCCHHHHHCCHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LVINQNKLEKLLSIVALYQDLGGGYRGH
HHHCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYRKRWLILLCALLNNGCLWLGSEYRKPHVNTPQKWPHKYSIQTEQAAYLPDLHWWKQFN
CCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHCC
SKELNASIQKALQNNHQIHLAMANIESAQGQLRQVQLSWLPNLTGLAGYTQFPVLGNPGT
CHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHEEHHHHCCCCCCCCCCCCCCEECCCCC
TAIAYPAYIINIFQQYKQQKSAQAMLEASIYAQYSARLVVIAQTAASFFTLVAQNEALHL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHCCCHHHH
YNKLLKDYRTYLKLTQSQYRSGLISLDNITQIKSHIQRIEAQIKVVQHNIVVTKNALHFL
HHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCEEEE
FNENPGDVETKTLFENIDSNQLVPGNLPASVLRVRPDIHEAEALLKAAHADVGAIAANLL
ECCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHC
PGINLGAYLGEGSNVDGAIKLGQAYLNGPIIDLPLFAQVDVSKARYKAIYIKYITTIRKA
CCCCCHHEECCCCCCCHHHHHHHHHHCCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHH
LRDVANDLSAYSTSSQQLNNNLSALSDEKQQCHLAEVRYRHGIDDYLNLIKCQILLDEFK
HHHHHHHHHHHCCCHHHHHCCHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LVINQNKLEKLLSIVALYQDLGGGYRGH
HHHCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]