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The map label for this gene is ywbI [H]

Identifier: 148358424

GI number: 148358424

Start: 303770

End: 304654

Strand: Direct

Name: ywbI [H]

Synonym: LPC_0290

Alternate gene names: 148358424

Gene position: 303770-304654 (Clockwise)

Preceding gene: 148358419

Following gene: 148358425

Centisome position: 8.49

GC content: 43.73

Gene sequence:

>885_bases
ATGGAATTTAAACTGCTTCAGACTTTCATAGAGGTTGTACGGCAGGGTAGTTTTTCCAAAGCAGCTGAAAATCTATTTGC
GACCCAGTCTACGGTGAGTAAAGCCATAAAACAGCTTGAAGAGGAGTTAGGTATCACTCTGATTGAGCGGTTTAAAAAGC
GTAATGTACCAACAACAGCAGGTGAAATTGTTTATCAGCGAGGAACCAAACTACTGGCTGATCGAGATGATCTTCTTAAA
GAATTGGATGAAATCCGAGGTTTGAAACAGGGTCGACTGCGTTTGGGTATTTCTCCGGTCGGCAGCAGTACTTTATTTGC
CCCTCTTTTTGTTCTGTACAGACAACGTTATCCAGGCATTGAAGTAGAGCTTGTTGAGCATGGCAGCGATAAACTTGCAG
AGTGTTTGCGTGCAGGAGAGATAGATTTTGCTGGAACCTTATTACCTATTTCCGAAGAATTTGATTGCCAATTAGTACGC
TCTGAACCGTTGGTTGCGCTCCTGGCATCTGGACATCCATTAGCAACTAAACATTGCATTTCTCTACCAGAACTTAAAGA
AATACCTTTCATCCTTTTTGGCAGTGGTTTCGCACTACATCGAATTATATTGGACGCGTGCCGTCACGCTGGTTTTGAAC
CTAAGGTGGTGGCTCAAAGCAGTCAGATTGATTTTATGCTGGAGCTTGTTGCAGCAGGCCTTGGGGTTACTTTTTTGCCA
CGGATGATTGCCACTCAAAGACATACGACACAAATTCGCTCTCTTTTACTTAATGGAAGTGAGTTCCAATGGAATATGGC
GATGACTTGGCGTAGAAATTCTTATCTCTCTGAGGCTGCGAAAGCCTGGTTGGCTCTGGTGCGCGAAGTGCATGTTGATA
ATTAG

Upstream 100 bases:

>100_bases
TGCTCATGGTGGATATTATTTTTTCATTTAAGTTTACACTTTTAATTAAGATGAATAAAATGAATTTATAGACTAATAAC
CTTTCCAATATGGAATAATT

Downstream 100 bases:

>100_bases
TTTTAGATTGGGGAATGCAAGAGATTTATTTTCCTATTGTCCAAAAGAGGCAGGTGTATTGAAAATCATTGCTGCTGTAG
CCATACTTCATATCTAAATT

Product: LysR family transporter transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MEFKLLQTFIEVVRQGSFSKAAENLFATQSTVSKAIKQLEEELGITLIERFKKRNVPTTAGEIVYQRGTKLLADRDDLLK
ELDEIRGLKQGRLRLGISPVGSSTLFAPLFVLYRQRYPGIEVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVR
SEPLVALLASGHPLATKHCISLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVAQSSQIDFMLELVAAGLGVTFLP
RMIATQRHTTQIRSLLLNGSEFQWNMAMTWRRNSYLSEAAKAWLALVREVHVDN

Sequences:

>Translated_294_residues
MEFKLLQTFIEVVRQGSFSKAAENLFATQSTVSKAIKQLEEELGITLIERFKKRNVPTTAGEIVYQRGTKLLADRDDLLK
ELDEIRGLKQGRLRLGISPVGSSTLFAPLFVLYRQRYPGIEVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVR
SEPLVALLASGHPLATKHCISLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVAQSSQIDFMLELVAAGLGVTFLP
RMIATQRHTTQIRSLLLNGSEFQWNMAMTWRRNSYLSEAAKAWLALVREVHVDN
>Mature_294_residues
MEFKLLQTFIEVVRQGSFSKAAENLFATQSTVSKAIKQLEEELGITLIERFKKRNVPTTAGEIVYQRGTKLLADRDDLLK
ELDEIRGLKQGRLRLGISPVGSSTLFAPLFVLYRQRYPGIEVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVR
SEPLVALLASGHPLATKHCISLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVAQSSQIDFMLELVAAGLGVTFLP
RMIATQRHTTQIRSLLLNGSEFQWNMAMTWRRNSYLSEAAKAWLALVREVHVDN

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=282, Percent_Identity=30.1418439716312, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1788887, Length=293, Percent_Identity=27.3037542662116, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1787879, Length=290, Percent_Identity=26.2068965517241, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790399, Length=279, Percent_Identity=24.7311827956989, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788748, Length=300, Percent_Identity=26, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI145693105, Length=295, Percent_Identity=24.406779661017, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787530, Length=246, Percent_Identity=24.7967479674797, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI2367136, Length=249, Percent_Identity=25.7028112449799, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=26.0162601626016, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789204, Length=247, Percent_Identity=23.4817813765182, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789506, Length=293, Percent_Identity=21.8430034129693, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787601, Length=171, Percent_Identity=28.0701754385965, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788296, Length=249, Percent_Identity=26.9076305220884, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790262, Length=254, Percent_Identity=27.5590551181102, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786401, Length=120, Percent_Identity=35, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788297, Length=247, Percent_Identity=25.5060728744939, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693193, Length=141, Percent_Identity=34.0425531914894, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788481, Length=204, Percent_Identity=26.9607843137255, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787589, Length=174, Percent_Identity=26.4367816091954, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789639, Length=123, Percent_Identity=32.520325203252, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787128, Length=173, Percent_Identity=30.0578034682081, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789440, Length=119, Percent_Identity=31.0924369747899, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32935; Mature: 32935

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKLLQTFIEVVRQGSFSKAAENLFATQSTVSKAIKQLEEELGITLIERFKKRNVPTTA
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCH
GEIVYQRGTKLLADRDDLLKELDEIRGLKQGRLRLGISPVGSSTLFAPLFVLYRQRYPGI
HHHHHHCCCHHHCCHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCC
EVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVRSEPLVALLASGHPLATKHCI
EEEEECCCCHHHHHHHHCCCCCCCCEEEECCCCCCCEEECCCCEEEEEECCCCCHHHHHC
SLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVAQSSQIDFMLELVAAGLGVTFLP
CCCCHHHCCEEEECCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCHHHHH
RMIATQRHTTQIRSLLLNGSEFQWNMAMTWRRNSYLSEAAKAWLALVREVHVDN
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFKLLQTFIEVVRQGSFSKAAENLFATQSTVSKAIKQLEEELGITLIERFKKRNVPTTA
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCH
GEIVYQRGTKLLADRDDLLKELDEIRGLKQGRLRLGISPVGSSTLFAPLFVLYRQRYPGI
HHHHHHCCCHHHCCHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCC
EVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVRSEPLVALLASGHPLATKHCI
EEEEECCCCHHHHHHHHCCCCCCCCEEEECCCCCCCEEECCCCEEEEEECCCCCHHHHHC
SLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVAQSSQIDFMLELVAAGLGVTFLP
CCCCHHHCCEEEECCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCHHHHH
RMIATQRHTTQIRSLLLNGSEFQWNMAMTWRRNSYLSEAAKAWLALVREVHVDN
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]