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The map label for this gene is traC [H]

Identifier: 148358322

GI number: 148358322

Start: 198265

End: 200451

Strand: Direct

Name: traC [H]

Synonym: LPC_0184

Alternate gene names: 148358322

Gene position: 198265-200451 (Clockwise)

Preceding gene: 148358321

Following gene: 148358327

Centisome position: 5.54

GC content: 42.89

Gene sequence:

>2187_bases
ATGACTAAGCTGTCCCATCATCAAATTGTCGGCAATCAAATCATTGAGAGTCTAAAACAAGGCACAGCGCCCTGGATCAA
GCCATGGGAGCCAGGCATTGGTAATGGACAAGTACCTTTTAATCCGGTAACTGGAAAACGATATCGCGGCATCAATGCCT
TGTATTTGATGTTGAATCAAAGCAATGATAATCGCTGGTTAACCTACAAACAGGCCCAAAGTATTGATGCACAAGTTAAA
AAAGGAGAAAAAGGTACGACTGTTCAATACTGGAAATTTCATGATGAAAAAATCAAAAAAGATGAAGCGGGCAATCCAGT
GCTGGATGAACAAGGAAACCCAGTCAAAGTTCAGGTTAATCTGGAAAGGCCAAAAGTGTTTTATGCCAGAGTTTTCCATG
CCTCACAAATTGACAACATGCCAGAACTTATTCAAAAAGAACAGGACTGGTCTTTAATTGAACGCGCTGAAACTCTGCTT
TTAAATTCTGGAGCTAAGATTTTTCATTCGGAAGCTGACCGAGCGTTTTATAGACTTTCAACTGACAGTATTCACCTGCC
GCCCAAAGAGCAATTTAAATCTGCCGCCAATTATTACGCTACAGCCCTTCATGAGCTAGGCCACTGGAGCGGCCACCCAT
CCAGGCTTGATCGGGACTTAAGCCACCCTTTTGGCAGTGAAGCGTATGCTAAAGAAGAATTAAGGGCTGAAATCGCCAGC
ATGCTATTGGGATCAGAGCTTGGCATCGGTCATGATCCTTCCCAACACACGGCTTATATCAAGTCATGGATTCGCATTAT
AGAAGATGACCCGCTGGAAATATTCAGAGCGTCAGCTGACGCGGAAAAAATTTTCAACCACATTTGCGCATTAAAGCAAG
TTCAAGAAATCCAATACGCGCAATCCGTTGAAATCAGGGATGAACAATTGAGAGAGGAAAATCTCATGGAAATTGACAAG
AAAATCTCTGATAAAATTTGGCTTAACATCCCATATAAGCAAAAAGATACTGCCAAGTCCGTAATTGGTAAATTGCCCGA
TGGGACACCTGGGATTGCCTGGGACAAAGAACAAAAGTGCTGGTATGCAAAACCTGGTGTTTCTTTTGATCGCATCAAAG
CCTGGCTTCCTGAAAATCAAGTTGCGTCAGAAGATAAAGCACTTTCACCTGTCGATGAGTTCAAAGAAGCATTAATCAAC
CTAGGGGCTATGGTGACAGGTGAGCACCCCATCATGGATGGAAAAACTCATCGAATACAAATGGAGGGCGATAAGCCTGG
CGAAAAAGCAGGATTTTACGTAGCCCATTTAGATGGTATTCCCGCAGGCTATATCAAAAATAACCGCACAGGCACGGAGT
TGAAATGGAAATGCAAAGGCTATGTTTTAACTGATGAGCAAAGAGCAGCATTTAAAGCTCAGGCACTTGAAAATCAGAAA
AATCGTGACATTGAGCTTGATGAAAAGCACAGAAGCACAGCTTTAAAACTCAAACATAAGCTATCCCAAATGAGCGAGGC
CTTTGAGCAAACGCCTTATATGAAATCTAAGGGTATTCAGGTGCACTCTGGTGTTTATACCGATAATGACAAAAAGCTCA
CCTGTATTCCTGCCACGGATATTGAAGGCACCATTTGGACGGTGCAATACATTGCAGAAGATGGAACCAAGCGCTTTGCC
AAAGACTCTAAAAAGGAAGGGTGTTTTCATGTATTAGGTGGCATCGAAAAGCTTGCTGATTCACCAGTTATTGTGATTGC
CGAAGGTTATGCGACCGCTGCCACCATAAAAGAAGCATGCGAGCTGCCAGCTGTCGTTTCTGCCTTTGATTCAGGAAATC
TAAAATCAGTGGCGAAAGCATTGCATGAAAAATATCCCCACACCCCAATTGTAATGGCTGCTGATGATGATAGACACCTT
GAACAATCGAAAGGTATCAATCCGGGCAAAGATAAAGCGGGTGAGGCTGCCAATGCTGTCAATGGCTTTATTGTTATACC
AACCTTCGCCCCGGATGAGCAGTCATCAAACCCTAGACAATTCAGTGATTTTAATGATTTGGCAAATCATAGCAAGTTAG
GCATTGAAGGGGTTAGGCGTCAGATTAAGCCTAAAGTTGAAAAAATTGCTAAACGATACAGCTGGCTGCGCAGGTCAATA
AAGAACAATGTGGTTAGCTTAGCATAA

Upstream 100 bases:

>100_bases
GTGCCTGGTTTTGTCGCTGAAATCAGTCCTTTAGAGGCAGAAATCATGGGTGCGTTTTCCGAAGAGGCGCTCAGTGAAAT
GGATGCTAAGGAGGCAGCCT

Downstream 100 bases:

>100_bases
GATTTGAGGCAGATTATGGCGGGCAAAAAAATAATATTTGAATTCTGCCCGCAAATTTTCAGTTAATACTGGAGTGCAAG
GCTATTTTAAATAACAAAAG

Product: DNA primase TraC

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 728; Mature: 727

Protein sequence:

>728_residues
MTKLSHHQIVGNQIIESLKQGTAPWIKPWEPGIGNGQVPFNPVTGKRYRGINALYLMLNQSNDNRWLTYKQAQSIDAQVK
KGEKGTTVQYWKFHDEKIKKDEAGNPVLDEQGNPVKVQVNLERPKVFYARVFHASQIDNMPELIQKEQDWSLIERAETLL
LNSGAKIFHSEADRAFYRLSTDSIHLPPKEQFKSAANYYATALHELGHWSGHPSRLDRDLSHPFGSEAYAKEELRAEIAS
MLLGSELGIGHDPSQHTAYIKSWIRIIEDDPLEIFRASADAEKIFNHICALKQVQEIQYAQSVEIRDEQLREENLMEIDK
KISDKIWLNIPYKQKDTAKSVIGKLPDGTPGIAWDKEQKCWYAKPGVSFDRIKAWLPENQVASEDKALSPVDEFKEALIN
LGAMVTGEHPIMDGKTHRIQMEGDKPGEKAGFYVAHLDGIPAGYIKNNRTGTELKWKCKGYVLTDEQRAAFKAQALENQK
NRDIELDEKHRSTALKLKHKLSQMSEAFEQTPYMKSKGIQVHSGVYTDNDKKLTCIPATDIEGTIWTVQYIAEDGTKRFA
KDSKKEGCFHVLGGIEKLADSPVIVIAEGYATAATIKEACELPAVVSAFDSGNLKSVAKALHEKYPHTPIVMAADDDRHL
EQSKGINPGKDKAGEAANAVNGFIVIPTFAPDEQSSNPRQFSDFNDLANHSKLGIEGVRRQIKPKVEKIAKRYSWLRRSI
KNNVVSLA

Sequences:

>Translated_728_residues
MTKLSHHQIVGNQIIESLKQGTAPWIKPWEPGIGNGQVPFNPVTGKRYRGINALYLMLNQSNDNRWLTYKQAQSIDAQVK
KGEKGTTVQYWKFHDEKIKKDEAGNPVLDEQGNPVKVQVNLERPKVFYARVFHASQIDNMPELIQKEQDWSLIERAETLL
LNSGAKIFHSEADRAFYRLSTDSIHLPPKEQFKSAANYYATALHELGHWSGHPSRLDRDLSHPFGSEAYAKEELRAEIAS
MLLGSELGIGHDPSQHTAYIKSWIRIIEDDPLEIFRASADAEKIFNHICALKQVQEIQYAQSVEIRDEQLREENLMEIDK
KISDKIWLNIPYKQKDTAKSVIGKLPDGTPGIAWDKEQKCWYAKPGVSFDRIKAWLPENQVASEDKALSPVDEFKEALIN
LGAMVTGEHPIMDGKTHRIQMEGDKPGEKAGFYVAHLDGIPAGYIKNNRTGTELKWKCKGYVLTDEQRAAFKAQALENQK
NRDIELDEKHRSTALKLKHKLSQMSEAFEQTPYMKSKGIQVHSGVYTDNDKKLTCIPATDIEGTIWTVQYIAEDGTKRFA
KDSKKEGCFHVLGGIEKLADSPVIVIAEGYATAATIKEACELPAVVSAFDSGNLKSVAKALHEKYPHTPIVMAADDDRHL
EQSKGINPGKDKAGEAANAVNGFIVIPTFAPDEQSSNPRQFSDFNDLANHSKLGIEGVRRQIKPKVEKIAKRYSWLRRSI
KNNVVSLA
>Mature_727_residues
TKLSHHQIVGNQIIESLKQGTAPWIKPWEPGIGNGQVPFNPVTGKRYRGINALYLMLNQSNDNRWLTYKQAQSIDAQVKK
GEKGTTVQYWKFHDEKIKKDEAGNPVLDEQGNPVKVQVNLERPKVFYARVFHASQIDNMPELIQKEQDWSLIERAETLLL
NSGAKIFHSEADRAFYRLSTDSIHLPPKEQFKSAANYYATALHELGHWSGHPSRLDRDLSHPFGSEAYAKEELRAEIASM
LLGSELGIGHDPSQHTAYIKSWIRIIEDDPLEIFRASADAEKIFNHICALKQVQEIQYAQSVEIRDEQLREENLMEIDKK
ISDKIWLNIPYKQKDTAKSVIGKLPDGTPGIAWDKEQKCWYAKPGVSFDRIKAWLPENQVASEDKALSPVDEFKEALINL
GAMVTGEHPIMDGKTHRIQMEGDKPGEKAGFYVAHLDGIPAGYIKNNRTGTELKWKCKGYVLTDEQRAAFKAQALENQKN
RDIELDEKHRSTALKLKHKLSQMSEAFEQTPYMKSKGIQVHSGVYTDNDKKLTCIPATDIEGTIWTVQYIAEDGTKRFAK
DSKKEGCFHVLGGIEKLADSPVIVIAEGYATAATIKEACELPAVVSAFDSGNLKSVAKALHEKYPHTPIVMAADDDRHLE
QSKGINPGKDKAGEAANAVNGFIVIPTFAPDEQSSNPRQFSDFNDLANHSKLGIEGVRRQIKPKVEKIAKRYSWLRRSIK
NNVVSLA

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4227

COG function: function code L; Antirestriction protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 81895; Mature: 81763

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLSHHQIVGNQIIESLKQGTAPWIKPWEPGIGNGQVPFNPVTGKRYRGINALYLMLNQ
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEEEEEEEEC
SNDNRWLTYKQAQSIDAQVKKGEKGTTVQYWKFHDEKIKKDEAGNPVLDEQGNPVKVQVN
CCCCEEEEEHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCCCCCCCCCEEEEEEE
LERPKVFYARVFHASQIDNMPELIQKEQDWSLIERAETLLLNSGAKIFHSEADRAFYRLS
CCCCEEEHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEC
TDSIHLPPKEQFKSAANYYATALHELGHWSGHPSRLDRDLSHPFGSEAYAKEELRAEIAS
CCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
MLLGSELGIGHDPSQHTAYIKSWIRIIEDDPLEIFRASADAEKIFNHICALKQVQEIQYA
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QSVEIRDEQLREENLMEIDKKISDKIWLNIPYKQKDTAKSVIGKLPDGTPGIAWDKEQKC
HHCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
WYAKPGVSFDRIKAWLPENQVASEDKALSPVDEFKEALINLGAMVTGEHPIMDGKTHRIQ
EEECCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCEEEE
MEGDKPGEKAGFYVAHLDGIPAGYIKNNRTGTELKWKCKGYVLTDEQRAAFKAQALENQK
ECCCCCCCHHCEEEEEECCCCCCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHCCC
NRDIELDEKHRSTALKLKHKLSQMSEAFEQTPYMKSKGIQVHSGVYTDNDKKLTCIPATD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCEEECCCCEEEEEECCC
IEGTIWTVQYIAEDGTKRFAKDSKKEGCFHVLGGIEKLADSPVIVIAEGYATAATIKEAC
CCCCEEEEEEEHHCCHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
ELPAVVSAFDSGNLKSVAKALHEKYPHTPIVMAADDDRHLEQSKGINPGKDKAGEAANAV
CCCHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCCCCCCCCCCHHHHC
NGFIVIPTFAPDEQSSNPRQFSDFNDLANHSKLGIEGVRRQIKPKVEKIAKRYSWLRRSI
CCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KNNVVSLA
HHCCEECC
>Mature Secondary Structure 
TKLSHHQIVGNQIIESLKQGTAPWIKPWEPGIGNGQVPFNPVTGKRYRGINALYLMLNQ
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEEEEEEEEC
SNDNRWLTYKQAQSIDAQVKKGEKGTTVQYWKFHDEKIKKDEAGNPVLDEQGNPVKVQVN
CCCCEEEEEHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCCCCCCCCCEEEEEEE
LERPKVFYARVFHASQIDNMPELIQKEQDWSLIERAETLLLNSGAKIFHSEADRAFYRLS
CCCCEEEHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEC
TDSIHLPPKEQFKSAANYYATALHELGHWSGHPSRLDRDLSHPFGSEAYAKEELRAEIAS
CCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
MLLGSELGIGHDPSQHTAYIKSWIRIIEDDPLEIFRASADAEKIFNHICALKQVQEIQYA
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QSVEIRDEQLREENLMEIDKKISDKIWLNIPYKQKDTAKSVIGKLPDGTPGIAWDKEQKC
HHCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
WYAKPGVSFDRIKAWLPENQVASEDKALSPVDEFKEALINLGAMVTGEHPIMDGKTHRIQ
EEECCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCEEEE
MEGDKPGEKAGFYVAHLDGIPAGYIKNNRTGTELKWKCKGYVLTDEQRAAFKAQALENQK
ECCCCCCCHHCEEEEEECCCCCCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHCCC
NRDIELDEKHRSTALKLKHKLSQMSEAFEQTPYMKSKGIQVHSGVYTDNDKKLTCIPATD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCEEECCCCEEEEEECCC
IEGTIWTVQYIAEDGTKRFAKDSKKEGCFHVLGGIEKLADSPVIVIAEGYATAATIKEAC
CCCCEEEEEEEHHCCHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
ELPAVVSAFDSGNLKSVAKALHEKYPHTPIVMAADDDRHLEQSKGINPGKDKAGEAANAV
CCCHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCCCCCCCCCCHHHHC
NGFIVIPTFAPDEQSSNPRQFSDFNDLANHSKLGIEGVRRQIKPKVEKIAKRYSWLRRSI
CCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KNNVVSLA
HHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]