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The map label for this gene is lpxD [H]

Identifier: 148358257

GI number: 148358257

Start: 123406

End: 124461

Strand: Reverse

Name: lpxD [H]

Synonym: LPC_0119

Alternate gene names: 148358257

Gene position: 124461-123406 (Counterclockwise)

Preceding gene: 148358259

Following gene: 148358256

Centisome position: 3.48

GC content: 41.0

Gene sequence:

>1056_bases
ATGAATTCTTCTCTTCGTGATATAGCAAACCTTGTTCAAGGTATGGTTATTGGCAATGATGCCATCACTGTTTCCACACT
ATCACCCATAGATAATATACTTCCCGGATCTTTAGTTTTCGCCGAAGGTGAAGACAATATCAAACTTGCTGAAAACTCTG
AAGCAGCAGCTATTCTTATTGGTCAAGGTACTATCGATTCCCCAAAGCCATTAATTCAGGTAAAAAACCCTTTTAAAGCA
TTTATTGCCTTACTCAACCATTTTTATCCCCCAAGGAGAATATCTCCGGGGGTACACCCCACTGCTGTAATAGGTGCCGA
AGTACAGTTAGGCGATGAAGTTTATGTTGGGCCATTCGTAGTCATTGAATCAGGAAGTATTATAGGCAACCACTCGGTGC
TCAAAAGCCATATTCATATCGGCCATAATGTGGTCATTGGAGATCATACAACCATTCACCCCCAAGTCACTATTTATGAT
AATTGCCGTATCGGTTCGAATGTCACTATCCATGCCTCTACAGTAATAGGTTCTGATGGCTTTGGGTATACTTTTGTTGA
TGGCCAACATCTCAAAGTACCTCATTCGGGATATGTGGTAATTGAAAATAATGTCGAAGTTGGAGCCAACACAGCGATAG
ACAAGGCCACATTAGGTGCTACAGTGATAGGTGAAGGTACAAAAATTGATAATTTGGTACAAATCGCTCACTCAGTCAAA
TTAGGCAAGCATAATATTATTTGCGCTTTTACTGGCATTGCCGGCAGTACCACTACCGGAAATAATGTCATCTTTGCCGC
CAACGTGGGAGTGAGTGACCATGTTCACATCGAGGATGAGGTCATATTAGGCGCACGTACTGGAGTTCCCCCACACAAAC
ACTTAAAAAAAGGAACAGTATATCTTGGTAATCCGGCAAAACCTAAAGATGTTGCGATTAAGCACGAGCTGTCAGTGAAC
CGTATCCCATTAATACGAAAAAACATTAAATCACTTACCGAGCAAGTGGCTGTAATTAACAAAAAACTCGATATTAAAGC
AAAAGAGGTAGAGTAA

Upstream 100 bases:

>100_bases
TTATTAATCTGAAAGCCATCTGAATCGAGGATAATTCACTCGAAAATGGACAATAAGAGGTATATAATCGCTCTATTTTG
CGGAATTGAAAGGTAAACCA

Downstream 100 bases:

>100_bases
CTATGAAACCGCCTCTGATTCTAATAGACGGATCTTCCTATTTCTTTAGAGCGTTTCATGCCCTTCCACCATTGACAAAC
TCCAAAGGCCAGCCTACAGG

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N- acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MNSSLRDIANLVQGMVIGNDAITVSTLSPIDNILPGSLVFAEGEDNIKLAENSEAAAILIGQGTIDSPKPLIQVKNPFKA
FIALLNHFYPPRRISPGVHPTAVIGAEVQLGDEVYVGPFVVIESGSIIGNHSVLKSHIHIGHNVVIGDHTTIHPQVTIYD
NCRIGSNVTIHASTVIGSDGFGYTFVDGQHLKVPHSGYVVIENNVEVGANTAIDKATLGATVIGEGTKIDNLVQIAHSVK
LGKHNIICAFTGIAGSTTTGNNVIFAANVGVSDHVHIEDEVILGARTGVPPHKHLKKGTVYLGNPAKPKDVAIKHELSVN
RIPLIRKNIKSLTEQVAVINKKLDIKAKEVE

Sequences:

>Translated_351_residues
MNSSLRDIANLVQGMVIGNDAITVSTLSPIDNILPGSLVFAEGEDNIKLAENSEAAAILIGQGTIDSPKPLIQVKNPFKA
FIALLNHFYPPRRISPGVHPTAVIGAEVQLGDEVYVGPFVVIESGSIIGNHSVLKSHIHIGHNVVIGDHTTIHPQVTIYD
NCRIGSNVTIHASTVIGSDGFGYTFVDGQHLKVPHSGYVVIENNVEVGANTAIDKATLGATVIGEGTKIDNLVQIAHSVK
LGKHNIICAFTGIAGSTTTGNNVIFAANVGVSDHVHIEDEVILGARTGVPPHKHLKKGTVYLGNPAKPKDVAIKHELSVN
RIPLIRKNIKSLTEQVAVINKKLDIKAKEVE
>Mature_351_residues
MNSSLRDIANLVQGMVIGNDAITVSTLSPIDNILPGSLVFAEGEDNIKLAENSEAAAILIGQGTIDSPKPLIQVKNPFKA
FIALLNHFYPPRRISPGVHPTAVIGAEVQLGDEVYVGPFVVIESGSIIGNHSVLKSHIHIGHNVVIGDHTTIHPQVTIYD
NCRIGSNVTIHASTVIGSDGFGYTFVDGQHLKVPHSGYVVIENNVEVGANTAIDKATLGATVIGEGTKIDNLVQIAHSVK
LGKHNIICAFTGIAGSTTTGNNVIFAANVGVSDHVHIEDEVILGARTGVPPHKHLKKGTVYLGNPAKPKDVAIKHELSVN
RIPLIRKNIKSLTEQVAVINKKLDIKAKEVE

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786376, Length=336, Percent_Identity=30.3571428571429, Blast_Score=142, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573 [H]

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 37285; Mature: 37285

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSLRDIANLVQGMVIGNDAITVSTLSPIDNILPGSLVFAEGEDNIKLAENSEAAAILI
CCCHHHHHHHHHHCEEECCCEEEEEECCCHHHCCCCCEEEECCCCCEEEECCCCEEEEEE
GQGTIDSPKPLIQVKNPFKAFIALLNHFYPPRRISPGVHPTAVIGAEVQLGDEVYVGPFV
ECCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCEEEECCEE
VIESGSIIGNHSVLKSHIHIGHNVVIGDHTTIHPQVTIYDNCRIGSNVTIHASTVIGSDG
EEECCCEEECHHHHHHHEEECCEEEECCCEEECCEEEEEECCCCCCEEEEEEEEEECCCC
FGYTFVDGQHLKVPHSGYVVIENNVEVGANTAIDKATLGATVIGEGTKIDNLVQIAHSVK
CCEEEECCCEEECCCCCEEEEECCEEECCCCCCCHHHCCEEEEECCCCHHHHHHHHHHHC
LGKHNIICAFTGIAGSTTTGNNVIFAANVGVSDHVHIEDEVILGARTGVPPHKHLKKGTV
CCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCEEEECCEEEEEECCCCCCHHHHCCCEE
YLGNPAKPKDVAIKHELSVNRIPLIRKNIKSLTEQVAVINKKLDIKAKEVE
EECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCC
>Mature Secondary Structure
MNSSLRDIANLVQGMVIGNDAITVSTLSPIDNILPGSLVFAEGEDNIKLAENSEAAAILI
CCCHHHHHHHHHHCEEECCCEEEEEECCCHHHCCCCCEEEECCCCCEEEECCCCEEEEEE
GQGTIDSPKPLIQVKNPFKAFIALLNHFYPPRRISPGVHPTAVIGAEVQLGDEVYVGPFV
ECCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCEEEECCEE
VIESGSIIGNHSVLKSHIHIGHNVVIGDHTTIHPQVTIYDNCRIGSNVTIHASTVIGSDG
EEECCCEEECHHHHHHHEEECCEEEECCCEEECCEEEEEECCCCCCEEEEEEEEEECCCC
FGYTFVDGQHLKVPHSGYVVIENNVEVGANTAIDKATLGATVIGEGTKIDNLVQIAHSVK
CCEEEECCCEEECCCCCEEEEECCEEECCCCCCCHHHCCEEEEECCCCHHHHHHHHHHHC
LGKHNIICAFTGIAGSTTTGNNVIFAANVGVSDHVHIEDEVILGARTGVPPHKHLKKGTV
CCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCEEEECCEEEEEECCCCCCHHHHCCCEE
YLGNPAKPKDVAIKHELSVNRIPLIRKNIKSLTEQVAVINKKLDIKAKEVE
EECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA