Click here to switch to the map view.

The map label for this gene is rlmL [H]

Identifier: 148358162

GI number: 148358162

Start: 27600

End: 29723

Strand: Direct

Name: rlmL [H]

Synonym: LPC_0023

Alternate gene names: 148358162

Gene position: 27600-29723 (Clockwise)

Preceding gene: 148358161

Following gene: 148358166

Centisome position: 0.77

GC content: 38.98

Gene sequence:

>2124_bases
ATGAATTACTCTCTATTCATTAGTTGTTCAAAAGGTTTGGAGTATTTGCTGGAAGACGAACTCAAGGGATTGGGGCTTCA
TGTGACTCAGGTGAGTCCTCAAGGTGTCTATGGTGAGGCTAGCCTGCCTGTAATTTATAATTTATGTTTATGGTCAAGAC
TTGCTAACAGAATACAGCTCATTTTATTTAGTGGCCATGCCGCAAAAGAGCAAGCAGTACATCAGTTATGTACTGATTTT
CATTGGCAAACTGTTTTTACCCATGATAAAACCATAGCCATTGAATTTCATGGTGCCTCTGAGCAAATTCGTAATACCAT
GTTTGGCGCTCAGATAGTCAAAGATGGAATAGTCGATCATTTCCGTCGCTTGAATGGGTCAAGGCCTTCTGTCGACAAAG
AAAAACCGCAAATTTTAATTCATGCTCATTTGAAAAATGATATTTTAACGGTGAGCTTTGACTTGGTTGGATATAGCCTG
CATCAAAGAGGATATCGTAAAAAAGCAGGCAAGGCTCCTTTAAAGGAAAACGTTGCAGCAGCCATGTTATTAAGGGCCAA
ATGGCCAGAGTTAGCCGCACAGGGATATGGCTTACACGATCCATTTTGTGGCTCTGGAACATTAGTTATAGAAGCAGCCA
TGATGGCAGCTCATATTGCCCCAGGTTTATTACGACAAGATCAATCCTTACAATATTGGGCTCGGCATCAGAGTTCTTTA
TGGGAAAAATTGCGAACTCAGGCATTACAGCAAGTGAAACCTCTGGCAGTTAAGCTAGTTGGTACTGACGCTGATGGCAA
AATAATTACTCTTGCGCGTTCCAATGCTGAGAGAGCAGGCGTATTACCTTTGGTTGAATTTAATACATTGCCGCTAAATG
CTTGTAGGCCAGGGACAAAAAAAGGATTAGTTGTTTGTAATCCACCTTACGGTGAGCGATTGGGGGAGGTAACTCAACTT
GTTCCTTTATATCAACAATTGGGTACTACATTACATACTTGTTATCAAGGTTGGCAAGCGGCTATTTTAACTTCCAGTCC
AGTTTTGGCTAAAGCATTAGGATTAAGAGCTGATAAGCAATATACTTTATATAATGGCCCATTAGAGTGCAAACTCTATT
GTTTGACTCTTTCTGCTGCAAATAAACTAAAAAATACCCCGAATGCCCCGCTTTCTGATAATGCACAAATGCTATTTAAC
CGATTGGAAAAAAACAGAAATCATTTACAGAAATGGGCAAGAAAAAATCAAATCACCTGTTATCGAATTTACGATGCGGA
TTTACCCGAATATGCCTATGCCATAGACATATATAATGACTATGCTGTACTGCAAGAATATGCGCCACCAGCCAGCATTC
CAGTACACAAGGCAGAAAAACGCAGCCTTGAGATGTTGCAAGTCGTTCCACGGGCCTTAGGTATTCACCCTGAAAAATTA
ATAGTTAAACAAAGAAAGCAGCAAAAGGGCTCAGAACAATATCAAAAAATAGGTAAAACCAGCCAAAGACTGATAGTGAC
AGAAGGCAAGGCGAAATTGATAGTGAATTTGTATGATTATCTTGATACAGGACTATTTCTGGATCATAGACTTATGCGCT
TGAAATTTGCTCAATTAGAGCCGGGAACTCGATTTTTGAACTGTTTTTGTTATACCGCCAGTGCAAGCGTCCATGCTGCC
TTGGCCGGTGCATTAACCACGAATGTTGATTTATCCAAAACGTATTTGCTTTGGGCGGAAGATAATTTTAGATTAAATGA
TATTGATTTATCCAAGCATCAATTTTTGCAATACGATTGTAAGGAATGGATGAAAATTACCAGGGATAAATTTGATGTTA
TTTTTTTAGATCCCCCCAGCTTTTCAAACTCAAAGCGTATGAGCGATATTTTAGACATTCAAAGAGATCATGTTTCTTTG
ATTAACATGGCAATGCGGTTGTTAAATCCTAATGGTGTTTTATATTTCTCAACTAATCTGCGCCAATTTAAGTTAGAGCC
AATGCTAAAAGAGAAATATGCGGTACAAGACATTACACCGCAGACCATCGATCAGGATTTTAAACGAAATAGCAAAATTC
ACCATTGTTTTAAAATTGTAATGCCACATTTTGCGGATAACTGA

Upstream 100 bases:

>100_bases
AATTAAGGCAACTGATTAAAAAGGCATTAGATGATCAGATGAAAGAGAAAAATACAGGCGCTGCTAAAGCACTGTTTCGC
TATTTAAGGTCTTTAATAAA

Downstream 100 bases:

>100_bases
ATAATTCAGTTATTTATTATTAGATCTCTTGCCGAGCTTCCCTTTCATAAGCGTGAAGCTCGACTCTCTTTAAGATTTGG
ATTTTGTTTTGGTTTTGCTA

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 707; Mature: 707

Protein sequence:

>707_residues
MNYSLFISCSKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPVIYNLCLWSRLANRIQLILFSGHAAKEQAVHQLCTDF
HWQTVFTHDKTIAIEFHGASEQIRNTMFGAQIVKDGIVDHFRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSL
HQRGYRKKAGKAPLKENVAAAMLLRAKWPELAAQGYGLHDPFCGSGTLVIEAAMMAAHIAPGLLRQDQSLQYWARHQSSL
WEKLRTQALQQVKPLAVKLVGTDADGKIITLARSNAERAGVLPLVEFNTLPLNACRPGTKKGLVVCNPPYGERLGEVTQL
VPLYQQLGTTLHTCYQGWQAAILTSSPVLAKALGLRADKQYTLYNGPLECKLYCLTLSAANKLKNTPNAPLSDNAQMLFN
RLEKNRNHLQKWARKNQITCYRIYDADLPEYAYAIDIYNDYAVLQEYAPPASIPVHKAEKRSLEMLQVVPRALGIHPEKL
IVKQRKQQKGSEQYQKIGKTSQRLIVTEGKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLEPGTRFLNCFCYTASASVHAA
LAGALTTNVDLSKTYLLWAEDNFRLNDIDLSKHQFLQYDCKEWMKITRDKFDVIFLDPPSFSNSKRMSDILDIQRDHVSL
INMAMRLLNPNGVLYFSTNLRQFKLEPMLKEKYAVQDITPQTIDQDFKRNSKIHHCFKIVMPHFADN

Sequences:

>Translated_707_residues
MNYSLFISCSKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPVIYNLCLWSRLANRIQLILFSGHAAKEQAVHQLCTDF
HWQTVFTHDKTIAIEFHGASEQIRNTMFGAQIVKDGIVDHFRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSL
HQRGYRKKAGKAPLKENVAAAMLLRAKWPELAAQGYGLHDPFCGSGTLVIEAAMMAAHIAPGLLRQDQSLQYWARHQSSL
WEKLRTQALQQVKPLAVKLVGTDADGKIITLARSNAERAGVLPLVEFNTLPLNACRPGTKKGLVVCNPPYGERLGEVTQL
VPLYQQLGTTLHTCYQGWQAAILTSSPVLAKALGLRADKQYTLYNGPLECKLYCLTLSAANKLKNTPNAPLSDNAQMLFN
RLEKNRNHLQKWARKNQITCYRIYDADLPEYAYAIDIYNDYAVLQEYAPPASIPVHKAEKRSLEMLQVVPRALGIHPEKL
IVKQRKQQKGSEQYQKIGKTSQRLIVTEGKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLEPGTRFLNCFCYTASASVHAA
LAGALTTNVDLSKTYLLWAEDNFRLNDIDLSKHQFLQYDCKEWMKITRDKFDVIFLDPPSFSNSKRMSDILDIQRDHVSL
INMAMRLLNPNGVLYFSTNLRQFKLEPMLKEKYAVQDITPQTIDQDFKRNSKIHHCFKIVMPHFADN
>Mature_707_residues
MNYSLFISCSKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPVIYNLCLWSRLANRIQLILFSGHAAKEQAVHQLCTDF
HWQTVFTHDKTIAIEFHGASEQIRNTMFGAQIVKDGIVDHFRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSL
HQRGYRKKAGKAPLKENVAAAMLLRAKWPELAAQGYGLHDPFCGSGTLVIEAAMMAAHIAPGLLRQDQSLQYWARHQSSL
WEKLRTQALQQVKPLAVKLVGTDADGKIITLARSNAERAGVLPLVEFNTLPLNACRPGTKKGLVVCNPPYGERLGEVTQL
VPLYQQLGTTLHTCYQGWQAAILTSSPVLAKALGLRADKQYTLYNGPLECKLYCLTLSAANKLKNTPNAPLSDNAQMLFN
RLEKNRNHLQKWARKNQITCYRIYDADLPEYAYAIDIYNDYAVLQEYAPPASIPVHKAEKRSLEMLQVVPRALGIHPEKL
IVKQRKQQKGSEQYQKIGKTSQRLIVTEGKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLEPGTRFLNCFCYTASASVHAA
LAGALTTNVDLSKTYLLWAEDNFRLNDIDLSKHQFLQYDCKEWMKITRDKFDVIFLDPPSFSNSKRMSDILDIQRDHVSL
INMAMRLLNPNGVLYFSTNLRQFKLEPMLKEKYAVQDITPQTIDQDFKRNSKIHHCFKIVMPHFADN

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=709, Percent_Identity=43.441466854725, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI145693111, Length=263, Percent_Identity=30.4182509505703, Blast_Score=92, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 80167; Mature: 80167

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYSLFISCSKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPVIYNLCLWSRLANRIQL
CCEEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEE
ILFSGHAAKEQAVHQLCTDFHWQTVFTHDKTIAIEFHGASEQIRNTMFGAQIVKDGIVDH
EEECCCCHHHHHHHHHHHHCCCEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
FRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSLHQRGYRKKAGKAPLKENVAA
HHHHCCCCCCCCCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHH
AMLLRAKWPELAAQGYGLHDPFCGSGTLVIEAAMMAAHIAPGLLRQDQSLQYWARHQSSL
HHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHH
WEKLRTQALQQVKPLAVKLVGTDADGKIITLARSNAERAGVLPLVEFNTLPLNACRPGTK
HHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCC
KGLVVCNPPYGERLGEVTQLVPLYQQLGTTLHTCYQGWQAAILTSSPVLAKALGLRADKQ
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCC
YTLYNGPLECKLYCLTLSAANKLKNTPNAPLSDNAQMLFNRLEKNRNHLQKWARKNQITC
EEEECCCCEEEEEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
YRIYDADLPEYAYAIDIYNDYAVLQEYAPPASIPVHKAEKRSLEMLQVVPRALGIHPEKL
EEEECCCCCCEEEEEEEECCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
IVKQRKQQKGSEQYQKIGKTSQRLIVTEGKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLE
HHHHHHHHCCHHHHHHHCCCCCEEEEECCCCEEEEEHHHHHCCCHHHHHHHHHHHHHCCC
PGTRFLNCFCYTASASVHAALAGALTTNVDLSKTYLLWAEDNFRLNDIDLSKHQFLQYDC
CCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEEECCCCCHHHHHHHHH
KEWMKITRDKFDVIFLDPPSFSNSKRMSDILDIQRDHVSLINMAMRLLNPNGVLYFSTNL
HHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
RQFKLEPMLKEKYAVQDITPQTIDQDFKRNSKIHHCFKIVMPHFADN
EEEECCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNYSLFISCSKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPVIYNLCLWSRLANRIQL
CCEEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEE
ILFSGHAAKEQAVHQLCTDFHWQTVFTHDKTIAIEFHGASEQIRNTMFGAQIVKDGIVDH
EEECCCCHHHHHHHHHHHHCCCEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
FRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSLHQRGYRKKAGKAPLKENVAA
HHHHCCCCCCCCCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHH
AMLLRAKWPELAAQGYGLHDPFCGSGTLVIEAAMMAAHIAPGLLRQDQSLQYWARHQSSL
HHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHH
WEKLRTQALQQVKPLAVKLVGTDADGKIITLARSNAERAGVLPLVEFNTLPLNACRPGTK
HHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCC
KGLVVCNPPYGERLGEVTQLVPLYQQLGTTLHTCYQGWQAAILTSSPVLAKALGLRADKQ
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCC
YTLYNGPLECKLYCLTLSAANKLKNTPNAPLSDNAQMLFNRLEKNRNHLQKWARKNQITC
EEEECCCCEEEEEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
YRIYDADLPEYAYAIDIYNDYAVLQEYAPPASIPVHKAEKRSLEMLQVVPRALGIHPEKL
EEEECCCCCCEEEEEEEECCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
IVKQRKQQKGSEQYQKIGKTSQRLIVTEGKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLE
HHHHHHHHCCHHHHHHHCCCCCEEEEECCCCEEEEEHHHHHCCCHHHHHHHHHHHHHCCC
PGTRFLNCFCYTASASVHAALAGALTTNVDLSKTYLLWAEDNFRLNDIDLSKHQFLQYDC
CCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEEECCCCCHHHHHHHHH
KEWMKITRDKFDVIFLDPPSFSNSKRMSDILDIQRDHVSLINMAMRLLNPNGVLYFSTNL
HHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
RQFKLEPMLKEKYAVQDITPQTIDQDFKRNSKIHHCFKIVMPHFADN
EEEECCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA