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The map label for this gene is yafV [C]

Identifier: 148358146

GI number: 148358146

Start: 10221

End: 11066

Strand: Reverse

Name: yafV [C]

Synonym: LPC_0007

Alternate gene names: 148358146

Gene position: 11066-10221 (Counterclockwise)

Preceding gene: 148358148

Following gene: 148358145

Centisome position: 0.31

GC content: 43.03

Gene sequence:

>846_bases
ATGAAAGAAAACAAACTAACGGTCGCACTGGTTCAGGAACAATGGTATGAAAATCCTAAAGAACATCAAGATAAACTGGC
TTCTGGAATATTTTCAGCTACCCAGCAAGGAGCAAAACTCGTTTGTTTACAAGAGCTTACCTTAAGCCCTTATTTCTGTA
CACGTAGTGACGTTGATCCCGCCCCTTATATGGAGGATATCGCCACTGGTCCCACTGCTCAATTTGTTAGCCAAATGGCA
AAGTCGAATCAAATTCATATTACCGCATCGCTTTTTGAAAAAGCTGGTTACAATACCGCGGTTGCTTTTAATCCCCAAGG
AGAATTGATTGCTGTTACCAGAAAGCAACATATCCCCAGTGGCGAAAAATATCATGAAAACTTTTACTTCAAGCCGGGCG
ATTCCAATTACCCTGTCCATACCATTGCTGGTCACTACTTGGGATTACCAACATGCTATGACCAATGGTTCCCCGAATTA
TCAAGAATTTATGGATTAAAAGGAGCTGAAATTCTGGTCTATCCCACCGCGATTGGAGGGGAACCGACTGCGCCAGGATT
TGATAGCCAACCCATGTGGCAAAAAGTTATGGTAGCACAAGGAATTATGAGCAACACCTTCATTATTGCGGTCAACCGGA
TTGGGTGTGAAGATGGTTTAAGTTTTTACGGAAGCAGCTTTATCAGTACCCCTATGGGCGAAATTTTAGTTCAGGCACCG
CGTAATGAACCTGCCGTATTGGTTGCTGAACTGGATTTTAGCCAACGTGAATTGTGGGGACGATTATTCCCATTTCCCCA
ACAACGAGAGCCTGAAACTTATCATGAATTAGTCAAATCCCGTTAA

Upstream 100 bases:

>100_bases
TAGCCAGATTTTTAATCTGGGTTGATGATTTAACTTAAGCAAGAGTAAGCCATTCGAATTCGAGATAAAAATTACTTAAC
ATCATTTATTGGGAACTCTA

Downstream 100 bases:

>100_bases
TCCTCTTTGGAACGTTAGAATGAACAATAATACTCTATCAGATGACAATCTTTACAACATAGAAAATTGGGGCGAGGGAT
ACTTCCGAATTAACTCCAAG

Product: amidohydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQELTLSPYFCTRSDVDPAPYMEDIATGPTAQFVSQMA
KSNQIHITASLFEKAGYNTAVAFNPQGELIAVTRKQHIPSGEKYHENFYFKPGDSNYPVHTIAGHYLGLPTCYDQWFPEL
SRIYGLKGAEILVYPTAIGGEPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCEDGLSFYGSSFISTPMGEILVQAP
RNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHELVKSR

Sequences:

>Translated_281_residues
MKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQELTLSPYFCTRSDVDPAPYMEDIATGPTAQFVSQMA
KSNQIHITASLFEKAGYNTAVAFNPQGELIAVTRKQHIPSGEKYHENFYFKPGDSNYPVHTIAGHYLGLPTCYDQWFPEL
SRIYGLKGAEILVYPTAIGGEPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCEDGLSFYGSSFISTPMGEILVQAP
RNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHELVKSR
>Mature_281_residues
MKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQELTLSPYFCTRSDVDPAPYMEDIATGPTAQFVSQMA
KSNQIHITASLFEKAGYNTAVAFNPQGELIAVTRKQHIPSGEKYHENFYFKPGDSNYPVHTIAGHYLGLPTCYDQWFPEL
SRIYGLKGAEILVYPTAIGGEPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCEDGLSFYGSSFISTPMGEILVQAP
RNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHELVKSR

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI7706509, Length=245, Percent_Identity=27.7551020408163, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI9910460, Length=281, Percent_Identity=29.1814946619217, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI297632348, Length=288, Percent_Identity=28.125, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI297632350, Length=288, Percent_Identity=28.125, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI5031947, Length=288, Percent_Identity=28.125, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17533173, Length=266, Percent_Identity=30.4511278195489, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323383, Length=298, Percent_Identity=27.8523489932886, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21358471, Length=264, Percent_Identity=28.7878787878788, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355835, Length=285, Percent_Identity=24.5614035087719, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI17933642, Length=222, Percent_Identity=28.3783783783784, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 31480; Mature: 31480

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQELTLSPYFCTRSDVDP
CCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCCCCCC
APYMEDIATGPTAQFVSQMAKSNQIHITASLFEKAGYNTAVAFNPQGELIAVTRKQHIPS
CCHHHHHCCCCHHHHHHHHHCCCCEEEEHHHHHHCCCCEEEEECCCCCEEEEEEHHCCCC
GEKYHENFYFKPGDSNYPVHTIAGHYLGLPTCYDQWFPELSRIYGLKGAEILVYPTAIGG
CCHHCCCCEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
EPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCEDGLSFYGSSFISTPMGEILVQAP
CCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHHEEECC
RNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHELVKSR
CCCCEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQELTLSPYFCTRSDVDP
CCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCCCCCC
APYMEDIATGPTAQFVSQMAKSNQIHITASLFEKAGYNTAVAFNPQGELIAVTRKQHIPS
CCHHHHHCCCCHHHHHHHHHCCCCEEEEHHHHHHCCCCEEEEECCCCCEEEEEEHHCCCC
GEKYHENFYFKPGDSNYPVHTIAGHYLGLPTCYDQWFPELSRIYGLKGAEILVYPTAIGG
CCHHCCCCEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
EPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCEDGLSFYGSSFISTPMGEILVQAP
CCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHHEEECC
RNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHELVKSR
CCCCEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]