Click here to switch to the map view.

The map label for this gene is pbpA1 [H]

Identifier: 148285175

GI number: 148285175

Start: 2118743

End: 2120491

Strand: Direct

Name: pbpA1 [H]

Synonym: OTBS_2173

Alternate gene names: 148285175

Gene position: 2118743-2120491 (Clockwise)

Preceding gene: 148285174

Following gene: 148285177

Centisome position: 99.61

GC content: 31.28

Gene sequence:

>1749_bases
ATGTTGAATAACAAAGTACATCAAAATAGATTGATTAATCGCAGAGCTTTTCTATTATTGATAGGGAAATTAGGTCTATT
ATGTATGTTAGCTGGTAGACTTTTTCACTTGCAAATTTTTCAATCTAGTAAATATAAAATTTTATCAGATAAAAATAGGA
TTAATATTGTATTATTAATAGCTAGGAGAGGCAAAATTTTAGATTGTAATGGTAATGTTTTAGCATTTAATAAGCGTGCT
TATCAAATTAAAGCAAATAAGAAGACTCTAGCTCAAAATCAATATTTTGCTCTTGATTTACTATATGATATTTTAAATTT
TAGTCACGAAAAGCGCGAAAAAATTAACCAAAAAATACTTGCTAGTAAAAATCTTTATGAAGTTCTTATTATGGATAATA
TTTCATGGGATGAGCTAGCTGCAATAGAAGAAAATTCATTAAATTTACCAGGTATATACATTGATTGTATGGAATATAGA
ACCTATACTTATCCATTATCAACATCACATATTTTAGGTTATACAGCATGGCCTAATGATGCTGAAAGGGTAAATTTAAA
TATAGAACACACTAGGATTCAAATTGGAAAAAGAGGTCTTGAAAAACATTTTGAAGAGGATCTTAGGGGAACTTGTGGCT
TTGAAAAGATTGAAGTAGATGCTAGAGGCCTAAAGGTTAGAGAGCTTGATACTATACCTAGTATTCCTGGTAAGGATATA
GCATTAAGCATTGATGTTAATTTGCAAAATAAACTTTATGAGATGTTTGATGATAGAGTAGGGGCTGCAATATTAACTGA
TGTAAATACTGGAAAGATTAAAGCTCTAGTTTCAGTTCCAGGTTATAATATTAATGCCTTTCCTAGAAGAATTAGTACAA
AGGACTGGAAAGCGCTTCTAGATAATCCTAATCTACCATTGGTTAGTCGTGCTTCACAATCATTATATCCTCCTGGTTCA
ATATTTCAATTAGTAACCGTACTTGCAGCTTTAGAAGCAGGAGTTAATTCTAAGTTAACTGTGTATTGTAATGGTAAAAG
CTTTATGAATGAGACTAATTTTAGCTGTTGGAATCATCGTGGGCATGGTAATATTGATATGCTTAGTGCAATTAAATATT
CGTGTAATAGTTATATTTATCATCTTGCTAGTTTGATTGGCTATAATGCTATTTTAGCAATGGCTAGAAAGTTAGGATTT
TGGAGAAGGGCTGTAGTAAATCTTCCGAATGAGCTAGTTGGAAATTTACCTACGCCAGAATGGAAAAAACGAAAAACAAA
ACAACGATGGATGTTAGGTGATACACTGAATATGGCTATTGGGCAAGGATATATTACTACTACTCCTTTGCAATTAGCAA
GAATGATTACTAGCATTGCTAATGGCAAGCAATTATTGCAGTTAAGTTTGACTTTAGATGAAGATGGTTATCATAAAAAT
TTGAATATTAATGCATGTTATATTGACTTTATTCATCAAGCTTTAAAGGCTGTTATTAATAATGAAAGTAGTGGTACTGC
TTATTCTTTGGATAGTGATAAAACTAAGCTAATGATTTCAGGTAAAACAGGTAGTGCTCAAGTAGGATCATCTTTGCAAA
GTCAAGCTCATGGGTTATTTGTTGGATTCTCTACTACTAATAAGCCTGAATATGCTGTTGCAGTGATTTTAGAACATGGT
GGACAAATCGGAATTAAATCTGCAGCTACAATTGCTAAAAGTATTTTGATTGAAGCATCAACTTTATAA

Upstream 100 bases:

>100_bases
AATTATCAGATTCTAATTTTCAAAATTCTAACTAGCAGCTTGTGTTATTTTCCTTTAAGCAAAATTTTCCAATATATTTT
TCGTAATTTAACACTCAGTG

Downstream 100 bases:

>100_bases
ACACCAGAATAAGATAAGGAGAAGTATGAAGATATAATCATAAAGGTTTGCAAGAGATAATATTTCATTATAACTTTTAA
TACATTACATTAAGCACCAC

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MLNNKVHQNRLINRRAFLLLIGKLGLLCMLAGRLFHLQIFQSSKYKILSDKNRINIVLLIARRGKILDCNGNVLAFNKRA
YQIKANKKTLAQNQYFALDLLYDILNFSHEKREKINQKILASKNLYEVLIMDNISWDELAAIEENSLNLPGIYIDCMEYR
TYTYPLSTSHILGYTAWPNDAERVNLNIEHTRIQIGKRGLEKHFEEDLRGTCGFEKIEVDARGLKVRELDTIPSIPGKDI
ALSIDVNLQNKLYEMFDDRVGAAILTDVNTGKIKALVSVPGYNINAFPRRISTKDWKALLDNPNLPLVSRASQSLYPPGS
IFQLVTVLAALEAGVNSKLTVYCNGKSFMNETNFSCWNHRGHGNIDMLSAIKYSCNSYIYHLASLIGYNAILAMARKLGF
WRRAVVNLPNELVGNLPTPEWKKRKTKQRWMLGDTLNMAIGQGYITTTPLQLARMITSIANGKQLLQLSLTLDEDGYHKN
LNINACYIDFIHQALKAVINNESSGTAYSLDSDKTKLMISGKTGSAQVGSSLQSQAHGLFVGFSTTNKPEYAVAVILEHG
GQIGIKSAATIAKSILIEASTL

Sequences:

>Translated_582_residues
MLNNKVHQNRLINRRAFLLLIGKLGLLCMLAGRLFHLQIFQSSKYKILSDKNRINIVLLIARRGKILDCNGNVLAFNKRA
YQIKANKKTLAQNQYFALDLLYDILNFSHEKREKINQKILASKNLYEVLIMDNISWDELAAIEENSLNLPGIYIDCMEYR
TYTYPLSTSHILGYTAWPNDAERVNLNIEHTRIQIGKRGLEKHFEEDLRGTCGFEKIEVDARGLKVRELDTIPSIPGKDI
ALSIDVNLQNKLYEMFDDRVGAAILTDVNTGKIKALVSVPGYNINAFPRRISTKDWKALLDNPNLPLVSRASQSLYPPGS
IFQLVTVLAALEAGVNSKLTVYCNGKSFMNETNFSCWNHRGHGNIDMLSAIKYSCNSYIYHLASLIGYNAILAMARKLGF
WRRAVVNLPNELVGNLPTPEWKKRKTKQRWMLGDTLNMAIGQGYITTTPLQLARMITSIANGKQLLQLSLTLDEDGYHKN
LNINACYIDFIHQALKAVINNESSGTAYSLDSDKTKLMISGKTGSAQVGSSLQSQAHGLFVGFSTTNKPEYAVAVILEHG
GQIGIKSAATIAKSILIEASTL
>Mature_582_residues
MLNNKVHQNRLINRRAFLLLIGKLGLLCMLAGRLFHLQIFQSSKYKILSDKNRINIVLLIARRGKILDCNGNVLAFNKRA
YQIKANKKTLAQNQYFALDLLYDILNFSHEKREKINQKILASKNLYEVLIMDNISWDELAAIEENSLNLPGIYIDCMEYR
TYTYPLSTSHILGYTAWPNDAERVNLNIEHTRIQIGKRGLEKHFEEDLRGTCGFEKIEVDARGLKVRELDTIPSIPGKDI
ALSIDVNLQNKLYEMFDDRVGAAILTDVNTGKIKALVSVPGYNINAFPRRISTKDWKALLDNPNLPLVSRASQSLYPPGS
IFQLVTVLAALEAGVNSKLTVYCNGKSFMNETNFSCWNHRGHGNIDMLSAIKYSCNSYIYHLASLIGYNAILAMARKLGF
WRRAVVNLPNELVGNLPTPEWKKRKTKQRWMLGDTLNMAIGQGYITTTPLQLARMITSIANGKQLLQLSLTLDEDGYHKN
LNINACYIDFIHQALKAVINNESSGTAYSLDSDKTKLMISGKTGSAQVGSSLQSQAHGLFVGFSTTNKPEYAVAVILEHG
GQIGIKSAATIAKSILIEASTL

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=602, Percent_Identity=30.3986710963455, Blast_Score=288, Evalue=6e-79,
Organism=Escherichia coli, GI1786272, Length=470, Percent_Identity=24.8936170212766, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 65148; Mature: 65148

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNNKVHQNRLINRRAFLLLIGKLGLLCMLAGRLFHLQIFQSSKYKILSDKNRINIVLLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEECCCCCEEEEEEE
ARRGKILDCNGNVLAFNKRAYQIKANKKTLAQNQYFALDLLYDILNFSHEKREKINQKIL
ECCCEEEECCCCEEEEECEEEEEECCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
ASKNLYEVLIMDNISWDELAAIEENSLNLPGIYIDCMEYRTYTYPLSTSHILGYTAWPND
CCCCEEEEEEECCCCHHHHHEECCCCCCCCEEEEEEEECEEEEECCCCCCEEEEECCCCC
AERVNLNIEHTRIQIGKRGLEKHFEEDLRGTCGFEKIEVDARGLKVRELDTIPSIPGKDI
CCEEEEEEEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCCCCCE
ALSIDVNLQNKLYEMFDDRVGAAILTDVNTGKIKALVSVPGYNINAFPRRISTKDWKALL
EEEEEECHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHH
DNPNLPLVSRASQSLYPPGSIFQLVTVLAALEAGVNSKLTVYCNGKSFMNETNFSCWNHR
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCCCEECCC
GHGNIDMLSAIKYSCNSYIYHLASLIGYNAILAMARKLGFWRRAVVNLPNELVGNLPTPE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCH
WKKRKTKQRWMLGDTLNMAIGQGYITTTPLQLARMITSIANGKQLLQLSLTLDEDGYHKN
HHHHHHHHHEECCCCHHHHHCCCEEEECHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCC
LNINACYIDFIHQALKAVINNESSGTAYSLDSDKTKLMISGKTGSAQVGSSLQSQAHGLF
CCEEEEHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCCEEE
VGFSTTNKPEYAVAVILEHGGQIGIKSAATIAKSILIEASTL
EEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MLNNKVHQNRLINRRAFLLLIGKLGLLCMLAGRLFHLQIFQSSKYKILSDKNRINIVLLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEECCCCCEEEEEEE
ARRGKILDCNGNVLAFNKRAYQIKANKKTLAQNQYFALDLLYDILNFSHEKREKINQKIL
ECCCEEEECCCCEEEEECEEEEEECCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
ASKNLYEVLIMDNISWDELAAIEENSLNLPGIYIDCMEYRTYTYPLSTSHILGYTAWPND
CCCCEEEEEEECCCCHHHHHEECCCCCCCCEEEEEEEECEEEEECCCCCCEEEEECCCCC
AERVNLNIEHTRIQIGKRGLEKHFEEDLRGTCGFEKIEVDARGLKVRELDTIPSIPGKDI
CCEEEEEEEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCCCCCE
ALSIDVNLQNKLYEMFDDRVGAAILTDVNTGKIKALVSVPGYNINAFPRRISTKDWKALL
EEEEEECHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHH
DNPNLPLVSRASQSLYPPGSIFQLVTVLAALEAGVNSKLTVYCNGKSFMNETNFSCWNHR
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCCCEECCC
GHGNIDMLSAIKYSCNSYIYHLASLIGYNAILAMARKLGFWRRAVVNLPNELVGNLPTPE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCH
WKKRKTKQRWMLGDTLNMAIGQGYITTTPLQLARMITSIANGKQLLQLSLTLDEDGYHKN
HHHHHHHHHEECCCCHHHHHCCCEEEECHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCC
LNINACYIDFIHQALKAVINNESSGTAYSLDSDKTKLMISGKTGSAQVGSSLQSQAHGLF
CCEEEEHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCCEEE
VGFSTTNKPEYAVAVILEHGGQIGIKSAATIAKSILIEASTL
EEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]