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The map label for this gene is priA [H]

Identifier: 148285048

GI number: 148285048

Start: 1874433

End: 1876373

Strand: Direct

Name: priA [H]

Synonym: OTBS_1948

Alternate gene names: 148285048

Gene position: 1874433-1876373 (Clockwise)

Preceding gene: 148285045

Following gene: 148285049

Centisome position: 88.12

GC content: 31.07

Gene sequence:

>1941_bases
ATGTTTATTAGAGTATTATTACCAAAGCTAAAGTTATTTCCGCTGGAGTATAAATCAACTACACATTTGTTATCCATTGG
AGATTTAGTAGTTGTCCCTTTTCGAAACAAGCAAATTACTGGTATTGTATGTGAAGTTAATATTATTCCACAACACAAAA
ACATTAAGGAAATTATAGCAAAGCAATTTCAAGGATATTCTATTAAGCCAATAAACTTAAAATTTATTGATATTGTAGCA
AGGTACTATATAGCAGATTTAGGAACTGTAGCAAAAATGACTTTACCTATTGAATTAAATGAGAAGCCATATGTTGCTGA
ATCGCAAGAGGTTAATCATAGTTTATTTCAGTTACTAGATCTGTCGCCAGAACAGCAGTTAGCATTTGATGCTATTAGAA
AAACTACAGGTGCAACCTTACTGCAAGGTATTACAGGATCAGGTAAAACAGAGATATATTTTCATTTGATTGCTGAGGCA
TTGAGACAGGGATTACAGGTACTTCTACTAATGCCGGAAATTAATCTTGCTGGTCATATAGCATTCAGATTTGCTAGTAG
ATTTAAATTTAATGCAGTTATATGGAATTCATCTGTTAGTAAATCTTTAAAGAAAAAAACACTCCGAGGAATATTAACTA
ATGAAATAAAAGTAGTTATTGGTACACGTAGTAGTATATTTTTACCATACTCATCGCTTGGATTAATTATAGTAGATGAA
GAACATGATCAATCATATAAACAAGAAAATAATATACTGTATAATGCTAGAGATATGGCGGTTATGAGAGGGCATATTGC
TAATATACCAGTAATTTTAGCTTCAGCAACTCCATCTCTTGAGAGCATATATAATACGTATAATAAGAAATACAGTCTTG
TTAAGCTTAAAAGTAGATTTGGCAGTTCTACTTTGCCTAATATCAATATTATTGATATGAAGAAGGAACATTTACCAAAT
GGAATGTGGTTATCTACTAGCTTAAAAAATGCTATGGAGCAAACTTTAAAACTGAATAAACAAATATTGCTGTTTTTAAA
CCGTAAAGGTTATGCTCCCATAATACTCTGTCGTAATTGTGGTTTTCGCTTTATGTGTTCATCATGTTCTTCATGCTTGG
TTTTTCATAAATCAAAAAATAAATTGCAATGTCATCATTGTGGTAGTTTTAAACCATTTGCCCAATCTTGTCCTGAATGT
AACGAACAAACATTATCAGCTTATGGACCTGGAATTGAGAGAATTGCTGAGGAAGTTAAAGTCTATTTTCCTAAATATAA
AACAGCGATTGTTAGCAGAGATACTACTAATACTATTGATAATAGTACTGAAATTTTACAGCAAATTATTAATAATGAAG
TAGATATTATCATTGGCACGCAAATGCTTACAAAAGGATACCATTTCCCAAATCTAAAACTAGTTGGAATAATTGATGCT
GACCTAGGATATGGCGGTGATTTAAGAGCTAATGAAAAGACATACCAGCTCCTTAACCAAGTAGCAGGAAGAGCTGGCAG
AGAAGAAGATAAAGGATATGTTTATATACAAACTTATTATCCTGATAGTTCACTATTAAAATATCTTATTAATTACAAAG
ATGATGATTTTATTAACTATGAAATGAATTTACGAATTAGTAATAATATGCCTCCAGCAACTAGATTTGTTGCTATTAGT
GTTATATCTACTTCGGAACAGAATGCTCAAGAAGTAGCAAAAAACATTGTTAGAACTGCTAAGCCATGTTCTAGTTTGAG
AATATTAGGCCCAGCTCCGGCGTTATTATACAAATTGAAAAATAAATATCGTTTTAGAATATTACTGATTTTTGAGCGAA
AACTAGATATTCGGAGCTACATCAAACACTGGAATATTTTGTCATTTCACTCTAAAAGAGTTAGCTTGAAAATAGATATT
GATCCATACAATTTCTATTAA

Upstream 100 bases:

>100_bases
CTTGATAATTTTTAATATGTCTGTTTATTGTACAGAATTTAATATTTGAGAATAGTATATTAATCTATAAATATACTTTA
AGCAAGTTTATTAATAACTC

Downstream 100 bases:

>100_bases
TAACTTTAGATTTTCTGTAAAATGAGAGATAGTAATATAAACATGAGTTCGATATAAAACATAGATCAGATAGTGTAAAA
GTATGTAGTCTTTATTTTGC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MFIRVLLPKLKLFPLEYKSTTHLLSIGDLVVVPFRNKQITGIVCEVNIIPQHKNIKEIIAKQFQGYSIKPINLKFIDIVA
RYYIADLGTVAKMTLPIELNEKPYVAESQEVNHSLFQLLDLSPEQQLAFDAIRKTTGATLLQGITGSGKTEIYFHLIAEA
LRQGLQVLLLMPEINLAGHIAFRFASRFKFNAVIWNSSVSKSLKKKTLRGILTNEIKVVIGTRSSIFLPYSSLGLIIVDE
EHDQSYKQENNILYNARDMAVMRGHIANIPVILASATPSLESIYNTYNKKYSLVKLKSRFGSSTLPNINIIDMKKEHLPN
GMWLSTSLKNAMEQTLKLNKQILLFLNRKGYAPIILCRNCGFRFMCSSCSSCLVFHKSKNKLQCHHCGSFKPFAQSCPEC
NEQTLSAYGPGIERIAEEVKVYFPKYKTAIVSRDTTNTIDNSTEILQQIINNEVDIIIGTQMLTKGYHFPNLKLVGIIDA
DLGYGGDLRANEKTYQLLNQVAGRAGREEDKGYVYIQTYYPDSSLLKYLINYKDDDFINYEMNLRISNNMPPATRFVAIS
VISTSEQNAQEVAKNIVRTAKPCSSLRILGPAPALLYKLKNKYRFRILLIFERKLDIRSYIKHWNILSFHSKRVSLKIDI
DPYNFY

Sequences:

>Translated_646_residues
MFIRVLLPKLKLFPLEYKSTTHLLSIGDLVVVPFRNKQITGIVCEVNIIPQHKNIKEIIAKQFQGYSIKPINLKFIDIVA
RYYIADLGTVAKMTLPIELNEKPYVAESQEVNHSLFQLLDLSPEQQLAFDAIRKTTGATLLQGITGSGKTEIYFHLIAEA
LRQGLQVLLLMPEINLAGHIAFRFASRFKFNAVIWNSSVSKSLKKKTLRGILTNEIKVVIGTRSSIFLPYSSLGLIIVDE
EHDQSYKQENNILYNARDMAVMRGHIANIPVILASATPSLESIYNTYNKKYSLVKLKSRFGSSTLPNINIIDMKKEHLPN
GMWLSTSLKNAMEQTLKLNKQILLFLNRKGYAPIILCRNCGFRFMCSSCSSCLVFHKSKNKLQCHHCGSFKPFAQSCPEC
NEQTLSAYGPGIERIAEEVKVYFPKYKTAIVSRDTTNTIDNSTEILQQIINNEVDIIIGTQMLTKGYHFPNLKLVGIIDA
DLGYGGDLRANEKTYQLLNQVAGRAGREEDKGYVYIQTYYPDSSLLKYLINYKDDDFINYEMNLRISNNMPPATRFVAIS
VISTSEQNAQEVAKNIVRTAKPCSSLRILGPAPALLYKLKNKYRFRILLIFERKLDIRSYIKHWNILSFHSKRVSLKIDI
DPYNFY
>Mature_646_residues
MFIRVLLPKLKLFPLEYKSTTHLLSIGDLVVVPFRNKQITGIVCEVNIIPQHKNIKEIIAKQFQGYSIKPINLKFIDIVA
RYYIADLGTVAKMTLPIELNEKPYVAESQEVNHSLFQLLDLSPEQQLAFDAIRKTTGATLLQGITGSGKTEIYFHLIAEA
LRQGLQVLLLMPEINLAGHIAFRFASRFKFNAVIWNSSVSKSLKKKTLRGILTNEIKVVIGTRSSIFLPYSSLGLIIVDE
EHDQSYKQENNILYNARDMAVMRGHIANIPVILASATPSLESIYNTYNKKYSLVKLKSRFGSSTLPNINIIDMKKEHLPN
GMWLSTSLKNAMEQTLKLNKQILLFLNRKGYAPIILCRNCGFRFMCSSCSSCLVFHKSKNKLQCHHCGSFKPFAQSCPEC
NEQTLSAYGPGIERIAEEVKVYFPKYKTAIVSRDTTNTIDNSTEILQQIINNEVDIIIGTQMLTKGYHFPNLKLVGIIDA
DLGYGGDLRANEKTYQLLNQVAGRAGREEDKGYVYIQTYYPDSSLLKYLINYKDDDFINYEMNLRISNNMPPATRFVAIS
VISTSEQNAQEVAKNIVRTAKPCSSLRILGPAPALLYKLKNKYRFRILLIFERKLDIRSYIKHWNILSFHSKRVSLKIDI
DPYNFY

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=537, Percent_Identity=36.1266294227188, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 73535; Mature: 73535

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIRVLLPKLKLFPLEYKSTTHLLSIGDLVVVPFRNKQITGIVCEVNIIPQHKNIKEIIA
CEEEEEECCHHEEEEECCCCEEEEEECCEEEEEECCCEEEEEEEEEEEECCCCCHHHHHH
KQFQGYSIKPINLKFIDIVARYYIADLGTVAKMTLPIELNEKPYVAESQEVNHSLFQLLD
HHHCCCCEEECCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHC
LSPEQQLAFDAIRKTTGATLLQGITGSGKTEIYFHLIAEALRQGLQVLLLMPEINLAGHI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCEEEEEECCCCCHHHH
AFRFASRFKFNAVIWNSSVSKSLKKKTLRGILTNEIKVVIGTRSSIFLPYSSLGLIIVDE
HEEHHHCEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCEEEEEC
EHDQSYKQENNILYNARDMAVMRGHIANIPVILASATPSLESIYNTYNKKYSLVKLKSRF
CCCHHHHHCCCEEEECHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCEEEEEEHHHC
GSSTLPNINIIDMKKEHLPNGMWLSTSLKNAMEQTLKLNKQILLFLNRKGYAPIILCRNC
CCCCCCCCEEEECHHHHCCCCEEEHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCC
GFRFMCSSCSSCLVFHKSKNKLQCHHCGSFKPFAQSCPECNEQTLSAYGPGIERIAEEVK
CCHHHHHCCCCEEEEECCCCCEEEEECCCCCHHHHCCCCCCHHHHHHCCCCHHHHHHHHH
VYFPKYKTAIVSRDTTNTIDNSTEILQQIINNEVDIIIGTQMLTKGYHFPNLKLVGIIDA
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECHHHHCCCCCCCEEEEEEEEC
DLGYGGDLRANEKTYQLLNQVAGRAGREEDKGYVYIQTYYPDSSLLKYLINYKDDDFINY
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEEE
EMNLRISNNMPPATRFVAISVISTSEQNAQEVAKNIVRTAKPCSSLRILGPAPALLYKLK
EEEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHC
NKYRFRILLIFERKLDIRSYIKHWNILSFHSKRVSLKIDIDPYNFY
CCCEEEEEEEEECCCCHHHHHHHCCEEEEECCEEEEEEEECCCCCC
>Mature Secondary Structure
MFIRVLLPKLKLFPLEYKSTTHLLSIGDLVVVPFRNKQITGIVCEVNIIPQHKNIKEIIA
CEEEEEECCHHEEEEECCCCEEEEEECCEEEEEECCCEEEEEEEEEEEECCCCCHHHHHH
KQFQGYSIKPINLKFIDIVARYYIADLGTVAKMTLPIELNEKPYVAESQEVNHSLFQLLD
HHHCCCCEEECCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHC
LSPEQQLAFDAIRKTTGATLLQGITGSGKTEIYFHLIAEALRQGLQVLLLMPEINLAGHI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCEEEEEECCCCCHHHH
AFRFASRFKFNAVIWNSSVSKSLKKKTLRGILTNEIKVVIGTRSSIFLPYSSLGLIIVDE
HEEHHHCEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCEEEEEC
EHDQSYKQENNILYNARDMAVMRGHIANIPVILASATPSLESIYNTYNKKYSLVKLKSRF
CCCHHHHHCCCEEEECHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCEEEEEEHHHC
GSSTLPNINIIDMKKEHLPNGMWLSTSLKNAMEQTLKLNKQILLFLNRKGYAPIILCRNC
CCCCCCCCEEEECHHHHCCCCEEEHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCC
GFRFMCSSCSSCLVFHKSKNKLQCHHCGSFKPFAQSCPECNEQTLSAYGPGIERIAEEVK
CCHHHHHCCCCEEEEECCCCCEEEEECCCCCHHHHCCCCCCHHHHHHCCCCHHHHHHHHH
VYFPKYKTAIVSRDTTNTIDNSTEILQQIINNEVDIIIGTQMLTKGYHFPNLKLVGIIDA
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECHHHHCCCCCCCEEEEEEEEC
DLGYGGDLRANEKTYQLLNQVAGRAGREEDKGYVYIQTYYPDSSLLKYLINYKDDDFINY
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCEEEE
EMNLRISNNMPPATRFVAISVISTSEQNAQEVAKNIVRTAKPCSSLRILGPAPALLYKLK
EEEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHC
NKYRFRILLIFERKLDIRSYIKHWNILSFHSKRVSLKIDIDPYNFY
CCCEEEEEEEEECCCCHHHHHHHCCEEEEECCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]