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The map label for this gene is spoT43 [H]

Identifier: 148285043

GI number: 148285043

Start: 1862942

End: 1863517

Strand: Direct

Name: spoT43 [H]

Synonym: OTBS_1936

Alternate gene names: 148285043

Gene position: 1862942-1863517 (Clockwise)

Preceding gene: 148285041

Following gene: 148285045

Centisome position: 87.58

GC content: 31.42

Gene sequence:

>576_bases
ATGATAGATTTTTATAGTGAGAGCTTAATAAATAAGCTGTTCAGAACCAACGTAAGATTTAACACCAAAATTGATCTTGA
TAGAGTTGAAAAAGCAATACTTTACGCTAAAAAATATCATGGCCAGCAAAAGAGAGATACTCAAGAACTATACTACACAC
ATCCATTAGAAGTAGCTTATATGGTTGCAGACTACAGCTTTGAAACAGATACGATTATTACAGCAATACTACATGATACA
CTTGAAGACACAAAACTAACTAAAGAAAGAATAAGGTACGAATTTGGTGCTAATATTGCAGAACAGGTTTCAGACCTTAC
CAGGGTTAGGGATAATAAGAAAATCAGTGCTATGGAAATGATACAAATATTACGCAGCCAAAATAAAACAGAACTATTAC
TGATCAAACTTTTTGATAGATTCCATAATATTCAAACCATACACATAAAACCTTATGGAAAAAGACAAAAAATCGTCATT
GAAACTCAGCAAGAATTTATACCTCTTGCTCAATATCTTAAATTACCAGAAATTACCATAGAGCTAAATAAATACTGTGA
GCTTTATGTTAGTTAG

Upstream 100 bases:

>100_bases
ATAATTTAATTTGCTTCTTTTTCAGTTTTTATCTGGGATTGGAGTATACAAAAAATTTAAATTATGGTATAATTTATTAA
TAACCATTTAGAGATGGAAA

Downstream 100 bases:

>100_bases
AATGCAAACTTTTTCTAACTAGCAAACATAGCATTAATATATATTTCGCTTTGCAGAATCAAGCATAGCTGCTTGGCATA
GATATATTATCGTTCAATTA

Product: spoT-like ppGpp hydrolase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MIDFYSESLINKLFRTNVRFNTKIDLDRVEKAILYAKKYHGQQKRDTQELYYTHPLEVAYMVADYSFETDTIITAILHDT
LEDTKLTKERIRYEFGANIAEQVSDLTRVRDNKKISAMEMIQILRSQNKTELLLIKLFDRFHNIQTIHIKPYGKRQKIVI
ETQQEFIPLAQYLKLPEITIELNKYCELYVS

Sequences:

>Translated_191_residues
MIDFYSESLINKLFRTNVRFNTKIDLDRVEKAILYAKKYHGQQKRDTQELYYTHPLEVAYMVADYSFETDTIITAILHDT
LEDTKLTKERIRYEFGANIAEQVSDLTRVRDNKKISAMEMIQILRSQNKTELLLIKLFDRFHNIQTIHIKPYGKRQKIVI
ETQQEFIPLAQYLKLPEITIELNKYCELYVS
>Mature_191_residues
MIDFYSESLINKLFRTNVRFNTKIDLDRVEKAILYAKKYHGQQKRDTQELYYTHPLEVAYMVADYSFETDTIITAILHDT
LEDTKLTKERIRYEFGANIAEQVSDLTRVRDNKKISAMEMIQILRSQNKTELLLIKLFDRFHNIQTIHIKPYGKRQKIVI
ETQQEFIPLAQYLKLPEITIELNKYCELYVS

Specific function: Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Plays a role in ada

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=169, Percent_Identity=34.3195266272189, Blast_Score=91, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 22777; Mature: 22777

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFYSESLINKLFRTNVRFNTKIDLDRVEKAILYAKKYHGQQKRDTQELYYTHPLEVAY
CCCCHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCHHHHEEECCHHEEE
MVADYSFETDTIITAILHDTLEDTKLTKERIRYEFGANIAEQVSDLTRVRDNKKISAMEM
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
IQILRSQNKTELLLIKLFDRFHNIQTIHIKPYGKRQKIVIETQQEFIPLAQYLKLPEITI
HHHHHCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCEEEEECHHHHHHHHHHHCCCCEEE
ELNKYCELYVS
EECCEEEEECC
>Mature Secondary Structure
MIDFYSESLINKLFRTNVRFNTKIDLDRVEKAILYAKKYHGQQKRDTQELYYTHPLEVAY
CCCCHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCHHHHEEECCHHEEE
MVADYSFETDTIITAILHDTLEDTKLTKERIRYEFGANIAEQVSDLTRVRDNKKISAMEM
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
IQILRSQNKTELLLIKLFDRFHNIQTIHIKPYGKRQKIVIETQQEFIPLAQYLKLPEITI
HHHHHCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCEEEEECHHHHHHHHHHHCCCCEEE
ELNKYCELYVS
EECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA