Click here to switch to the map view.

The map label for this gene is maeA [C]

Identifier: 148284721

GI number: 148284721

Start: 1279591

End: 1280919

Strand: Direct

Name: maeA [C]

Synonym: OTBS_1313

Alternate gene names: 148284721

Gene position: 1279591-1280919 (Clockwise)

Preceding gene: 148284720

Following gene: 148284723

Centisome position: 60.16

GC content: 34.01

Gene sequence:

>1329_bases
TTGCTTAAAATTAATCGGATAATCTTTATGACTAATAACTTATCTAAAAATGATTCATTAATCTCAAAGTTAAGTCTTGA
ATATCATAAAAAAGATAACATGCCAGGTAAAATAGCTATGGTAACTACCAAACCACTTGCAACTCAACACGACTTAGCGC
TAGCTTATTCCCCTGGTGTATCTGCTCCATGTCTAAAGATTTTTGATAATATTAATGCAATTTATGACTACACCTCTAGA
GGTAACACAGTTGCAGTAATTTCAAATGGCACAGCAGTTCTAGGGCTAGGCAATTTAGGAGCTGCTGCTGCTAAACCTGT
TATGGAAGGTAAGGCAGCATTATTTAAAAAATTTGCTGATATTGATGCTATAGATCTTGAGGTAGATACTGAAGATCCTT
ATGAAATTATTAAGATTATTCAATATCTTAAATATAATTGGGGTGGTATTAATTTAGAAGATATTAAGGCTCCTGAATGC
TTTATTATAGAAAATAAGCTACAAGAACTTGTAGATATTCCAGTATTTCATGATGACCAACATGGTACAGCTGTTGCAGT
TACAGCAGGAGTTATTAATGCTGCTGATCTTACAGGACGTCAGAATTTAAGAAATTTAAAAGTTATAATTAATGGAGCTG
GAGCTGCTGGCATTGCTTGTGCAGAATTATTAAAGTCTATAGGTATAGAAAATATAATATTATGCGATAGCCAGGGCGTA
ATTTATAAAGGACGAACTAAAGGCATGAATGCATGGAAAGAGGCTCATGCTATCGATACTAAGTATAGAACTCTCAAAGA
AGCAATAATTGGTGCTGATTTATTTTTAGGGTTATCAGTTAAAGGCACTTTAACACAAGAAATGTTAGCTACTATGGCTG
ATAAGCCAATTATCTTTGCCTTAGCTAATCCAGATCCTGAAATTATGCCTGATGAAGTTAAGTTTGTACGTCCAAATGCT
ATTGTTGCTACAGGAAGATCTGATTTTAGTAACCAAATAAATAATGTAATAGTGTTTCCTTATATTTTTAGAGGGGCATT
AGATGCTATGGCAACAACAATAAATAACTCAATGAAAATAGCTGCAGCTAAGGAAATAGCAGCGCTTGCTGCTTGTCCAG
TGCCAGAGTTAGTTACTAAAGCTCATTTAGGTCGAAAAATGGAATATGGCTTCGATTACATTCTTCCTTCACCATTTGAC
CCAAGGCTACTTTATACTATTCCACCATTAGTAGCTAGTGCAGCAGTAAAAACTGGAGTAGCTAGAAAAAATATTGATCT
TCAAAAGTATAAAAATTATCTTTTTGATAAAGCTGCATTTGCTGAGTAA

Upstream 100 bases:

>100_bases
CTTTCTCGACAACTACATGGAGATCCTGACTCCATGGCAACAATCATTACTTTTACTACAATATTTTCCTTATTGTCTTT
ATCAATTATTATGTATATCT

Downstream 100 bases:

>100_bases
ATAGTTATATAAGTAGCTATAATAACGTAGATAATAGTAAAGAAGGTGGCAATTTTTTTTGTACACATCTAAACTTTGCA
AATTCTTAAAGCTATTTAAT

Product: NADP-dependent malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MLKINRIIFMTNNLSKNDSLISKLSLEYHKKDNMPGKIAMVTTKPLATQHDLALAYSPGVSAPCLKIFDNINAIYDYTSR
GNTVAVISNGTAVLGLGNLGAAAAKPVMEGKAALFKKFADIDAIDLEVDTEDPYEIIKIIQYLKYNWGGINLEDIKAPEC
FIIENKLQELVDIPVFHDDQHGTAVAVTAGVINAADLTGRQNLRNLKVIINGAGAAGIACAELLKSIGIENIILCDSQGV
IYKGRTKGMNAWKEAHAIDTKYRTLKEAIIGADLFLGLSVKGTLTQEMLATMADKPIIFALANPDPEIMPDEVKFVRPNA
IVATGRSDFSNQINNVIVFPYIFRGALDAMATTINNSMKIAAAKEIAALAACPVPELVTKAHLGRKMEYGFDYILPSPFD
PRLLYTIPPLVASAAVKTGVARKNIDLQKYKNYLFDKAAFAE

Sequences:

>Translated_442_residues
MLKINRIIFMTNNLSKNDSLISKLSLEYHKKDNMPGKIAMVTTKPLATQHDLALAYSPGVSAPCLKIFDNINAIYDYTSR
GNTVAVISNGTAVLGLGNLGAAAAKPVMEGKAALFKKFADIDAIDLEVDTEDPYEIIKIIQYLKYNWGGINLEDIKAPEC
FIIENKLQELVDIPVFHDDQHGTAVAVTAGVINAADLTGRQNLRNLKVIINGAGAAGIACAELLKSIGIENIILCDSQGV
IYKGRTKGMNAWKEAHAIDTKYRTLKEAIIGADLFLGLSVKGTLTQEMLATMADKPIIFALANPDPEIMPDEVKFVRPNA
IVATGRSDFSNQINNVIVFPYIFRGALDAMATTINNSMKIAAAKEIAALAACPVPELVTKAHLGRKMEYGFDYILPSPFD
PRLLYTIPPLVASAAVKTGVARKNIDLQKYKNYLFDKAAFAE
>Mature_442_residues
MLKINRIIFMTNNLSKNDSLISKLSLEYHKKDNMPGKIAMVTTKPLATQHDLALAYSPGVSAPCLKIFDNINAIYDYTSR
GNTVAVISNGTAVLGLGNLGAAAAKPVMEGKAALFKKFADIDAIDLEVDTEDPYEIIKIIQYLKYNWGGINLEDIKAPEC
FIIENKLQELVDIPVFHDDQHGTAVAVTAGVINAADLTGRQNLRNLKVIINGAGAAGIACAELLKSIGIENIILCDSQGV
IYKGRTKGMNAWKEAHAIDTKYRTLKEAIIGADLFLGLSVKGTLTQEMLATMADKPIIFALANPDPEIMPDEVKFVRPNA
IVATGRSDFSNQINNVIVFPYIFRGALDAMATTINNSMKIAAAKEIAALAACPVPELVTKAHLGRKMEYGFDYILPSPFD
PRLLYTIPPLVASAAVKTGVARKNIDLQKYKNYLFDKAAFAE

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=383, Percent_Identity=25.5874673629243, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI62420880, Length=383, Percent_Identity=25.5874673629243, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI239049447, Length=383, Percent_Identity=25.5874673629243, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4505143, Length=345, Percent_Identity=27.2463768115942, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788806, Length=438, Percent_Identity=53.1963470319635, Blast_Score=451, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322823, Length=231, Percent_Identity=31.1688311688312, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI78707242, Length=416, Percent_Identity=26.4423076923077, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI78707238, Length=416, Percent_Identity=26.4423076923077, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24646388, Length=411, Percent_Identity=26.7639902676399, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24646386, Length=411, Percent_Identity=26.7639902676399, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI78707240, Length=349, Percent_Identity=27.5071633237822, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 48050; Mature: 48050

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKINRIIFMTNNLSKNDSLISKLSLEYHKKDNMPGKIAMVTTKPLATQHDLALAYSPGV
CEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEECCCC
SAPCLKIFDNINAIYDYTSRGNTVAVISNGTAVLGLGNLGAAAAKPVMEGKAALFKKFAD
CCHHHHHHHCCHHEEEECCCCCEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
IDAIDLEVDTEDPYEIIKIIQYLKYNWGGINLEDIKAPECFIIENKLQELVDIPVFHDDQ
CCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEEHHHHHHHHCCCCEECCC
HGTAVAVTAGVINAADLTGRQNLRNLKVIINGAGAAGIACAELLKSIGIENIILCDSQGV
CCCEEEEEECCEECHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCC
IYKGRTKGMNAWKEAHAIDTKYRTLKEAIIGADLFLGLSVKGTLTQEMLATMADKPIIFA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCCEEEE
LANPDPEIMPDEVKFVRPNAIVATGRSDFSNQINNVIVFPYIFRGALDAMATTINNSMKI
EECCCCCCCCCCCEEECCCEEEEECCHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCEEE
AAAKEIAALAACPVPELVTKAHLGRKMEYGFDYILPSPFDPRLLYTIPPLVASAAVKTGV
HHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCEECCCCCCCEEEEECCHHHHHHHHHHCC
ARKNIDLQKYKNYLFDKAAFAE
HHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKINRIIFMTNNLSKNDSLISKLSLEYHKKDNMPGKIAMVTTKPLATQHDLALAYSPGV
CEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEECCCC
SAPCLKIFDNINAIYDYTSRGNTVAVISNGTAVLGLGNLGAAAAKPVMEGKAALFKKFAD
CCHHHHHHHCCHHEEEECCCCCEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
IDAIDLEVDTEDPYEIIKIIQYLKYNWGGINLEDIKAPECFIIENKLQELVDIPVFHDDQ
CCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEEHHHHHHHHCCCCEECCC
HGTAVAVTAGVINAADLTGRQNLRNLKVIINGAGAAGIACAELLKSIGIENIILCDSQGV
CCCEEEEEECCEECHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCC
IYKGRTKGMNAWKEAHAIDTKYRTLKEAIIGADLFLGLSVKGTLTQEMLATMADKPIIFA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCCEEEE
LANPDPEIMPDEVKFVRPNAIVATGRSDFSNQINNVIVFPYIFRGALDAMATTINNSMKI
EECCCCCCCCCCCEEECCCEEEEECCHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCEEE
AAAKEIAALAACPVPELVTKAHLGRKMEYGFDYILPSPFDPRLLYTIPPLVASAAVKTGV
HHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCEECCCCCCCEEEEECCHHHHHHHHHHCC
ARKNIDLQKYKNYLFDKAAFAE
HHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]