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The map label for this gene is tsf

Identifier: 148284633

GI number: 148284633

Start: 1121240

End: 1122169

Strand: Direct

Name: tsf

Synonym: OTBS_1139

Alternate gene names: 148284633

Gene position: 1121240-1122169 (Clockwise)

Preceding gene: 148284632

Following gene: 148284636

Centisome position: 52.71

GC content: 34.09

Gene sequence:

>930_bases
ATGAAAGAAAAAGAATTAGTGGTTAATACTAAAACTAATGGATCTAAATCCGAAGATGTAATTATAAGTATTAGCTTAGT
CAAGAAGTTACGCGATGCTACTGGCGCTGCTATGACTAGCTGTAAGCAAGCATTGCAGGAGGCTAAAGGTGATATTGAAG
AAGCTATTAAAGTTTTACGTAAGACTAACTTAGCTCAAATGTCTTCAAAGTTACAACGTAAAGCTGACGAGGGAATAGTA
GCATTAGCTGTAGATAACAACAAAGGGGCAATTATAGAAATTAAGTTTGAAACAGATTTTGTTGCACGAAATGAAAGATT
GCAAAAATTAGCTATGGAAGCTGCTCAACTAGCTTTAGTGCATGATAATTTAACAGACTTAAAAAACGCTAAATTATCAT
CAGGAGAGACTCTTGAATCTCAAATAAGCCAAGATATTGCTGTTATAGGCGAAAATATACAAGTAAGCAGAATTAGACAA
GTTAAGCTAGTTGACAATGGTATTATAGCATCATATATACATAATAGCGTAGCTCCTAATTTAGGGCAGATTGCTGTATT
AGTAGCTTTAGAAGGTAATGTTGAATCAGACAAGCTGACAAAACTAGGAAAAAATATAGCAATGCATATTGCTGCTACTA
ATCCTAGGTTTTTAAGTTCAAATGAGGTACCAGAAAGTGTAATTATGCAAGAAAAGGAAATTTTTACCGCTCAAGCTCGT
ACTACTGGCAAACCAGAAAAAGTAATTGAAAAAATGATTGAGGGGCGAGTAAGTAAGTTTCTAGAAGAAACTGTGTTGCT
AGAGCAAGCTTTTGTTATTGATGGTAAAACAAAAATTTCTGAAGTGTTGAGTAATGCAGCTAAAGAACTTGGCAGTGAAA
TTAAGATTACTGGATTTTCTTGCTTAAAAGCTGGAGAAGTAGTAGAATAG

Upstream 100 bases:

>100_bases
AAGCTGCTAAAAAAATTACTAAAATGAAACTTAGTAAAAAAGTAACAAAAATAAATGTTGAACAAGATCAAAATAAATTA
GAAAAAGATAATAAAGATTT

Downstream 100 bases:

>100_bases
TGACTAAGAATTTTTTCTTAGTCCTTGTAAGAATTAAAGCTTGTTACGTTAAATGGTAGAGAATACGACTTTTTAATAGC
TAATGATCTCTCAATACCGC

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKEKELVVNTKTNGSKSEDVIISISLVKKLRDATGAAMTSCKQALQEAKGDIEEAIKVLRKTNLAQMSSKLQRKADEGIV
ALAVDNNKGAIIEIKFETDFVARNERLQKLAMEAAQLALVHDNLTDLKNAKLSSGETLESQISQDIAVIGENIQVSRIRQ
VKLVDNGIIASYIHNSVAPNLGQIAVLVALEGNVESDKLTKLGKNIAMHIAATNPRFLSSNEVPESVIMQEKEIFTAQAR
TTGKPEKVIEKMIEGRVSKFLEETVLLEQAFVIDGKTKISEVLSNAAKELGSEIKITGFSCLKAGEVVE

Sequences:

>Translated_309_residues
MKEKELVVNTKTNGSKSEDVIISISLVKKLRDATGAAMTSCKQALQEAKGDIEEAIKVLRKTNLAQMSSKLQRKADEGIV
ALAVDNNKGAIIEIKFETDFVARNERLQKLAMEAAQLALVHDNLTDLKNAKLSSGETLESQISQDIAVIGENIQVSRIRQ
VKLVDNGIIASYIHNSVAPNLGQIAVLVALEGNVESDKLTKLGKNIAMHIAATNPRFLSSNEVPESVIMQEKEIFTAQAR
TTGKPEKVIEKMIEGRVSKFLEETVLLEQAFVIDGKTKISEVLSNAAKELGSEIKITGFSCLKAGEVVE
>Mature_309_residues
MKEKELVVNTKTNGSKSEDVIISISLVKKLRDATGAAMTSCKQALQEAKGDIEEAIKVLRKTNLAQMSSKLQRKADEGIV
ALAVDNNKGAIIEIKFETDFVARNERLQKLAMEAAQLALVHDNLTDLKNAKLSSGETLESQISQDIAVIGENIQVSRIRQ
VKLVDNGIIASYIHNSVAPNLGQIAVLVALEGNVESDKLTKLGKNIAMHIAATNPRFLSSNEVPESVIMQEKEIFTAQAR
TTGKPEKVIEKMIEGRVSKFLEETVLLEQAFVIDGKTKISEVLSNAAKELGSEIKITGFSCLKAGEVVE

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=217, Percent_Identity=29.0322580645161, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI291084502, Length=162, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1786366, Length=293, Percent_Identity=36.1774744027304, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17561440, Length=297, Percent_Identity=26.5993265993266, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921466, Length=278, Percent_Identity=26.2589928057554, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_ORITB (A5CE05)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248723.1
- ProteinModelPortal:   A5CE05
- SMR:   A5CE05
- STRING:   A5CE05
- GeneID:   5219729
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_1139
- eggNOG:   COG0264
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- BioCyc:   OTSU357244:OTBS_1139-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 33610; Mature: 33610

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKELVVNTKTNGSKSEDVIISISLVKKLRDATGAAMTSCKQALQEAKGDIEEAIKVLR
CCCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KTNLAQMSSKLQRKADEGIVALAVDNNKGAIIEIKFETDFVARNERLQKLAMEAAQLALV
HCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
HDNLTDLKNAKLSSGETLESQISQDIAVIGENIQVSRIRQVKLVDNGIIASYIHNSVAPN
HCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHEEEEECCCCHHHHHHHHHCCCC
LGQIAVLVALEGNVESDKLTKLGKNIAMHIAATNPRFLSSNEVPESVIMQEKEIFTAQAR
CCCEEEEEEECCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
TTGKPEKVIEKMIEGRVSKFLEETVLLEQAFVIDGKTKISEVLSNAAKELGSEIKITGFS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHCCCCEEEECHH
CLKAGEVVE
HHCCCCCCC
>Mature Secondary Structure
MKEKELVVNTKTNGSKSEDVIISISLVKKLRDATGAAMTSCKQALQEAKGDIEEAIKVLR
CCCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KTNLAQMSSKLQRKADEGIVALAVDNNKGAIIEIKFETDFVARNERLQKLAMEAAQLALV
HCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
HDNLTDLKNAKLSSGETLESQISQDIAVIGENIQVSRIRQVKLVDNGIIASYIHNSVAPN
HCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHEEEEECCCCHHHHHHHHHCCCC
LGQIAVLVALEGNVESDKLTKLGKNIAMHIAATNPRFLSSNEVPESVIMQEKEIFTAQAR
CCCEEEEEEECCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
TTGKPEKVIEKMIEGRVSKFLEETVLLEQAFVIDGKTKISEVLSNAAKELGSEIKITGFS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHCCCCEEEECHH
CLKAGEVVE
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA