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The map label for this gene is gidB

Identifier: 148284582

GI number: 148284582

Start: 1029162

End: 1029758

Strand: Direct

Name: gidB

Synonym: OTBS_1039

Alternate gene names: 148284582

Gene position: 1029162-1029758 (Clockwise)

Preceding gene: 148284579

Following gene: 148284583

Centisome position: 48.38

GC content: 25.29

Gene sequence:

>597_bases
ATGCTTCAGCAAATATATGATTTTTTAAATATTAATTATAATGTTTCACGTGAAACATTTAATAAATTTAAAGAGTATTA
TTCTCTATTAAGCAAATGGAATTCAACAATTAATTTAGTTTCAGCAACTACTTTACAAAATTTTTGGCAAAGGCACATTT
TTGACTCTATACAATTATTAAATTATATTCATAATAAAGATATTATTGTTGCAGATTTAGGTAGTGGAGCTGGATTCCCT
GGAGTAGTACTATCTATATCAGGAATAAAAAATGTAATATTAATAGAATCTGATAGTCGCAAAGCTGCTTTTTTACTACA
AGCAGCTCAATTATCTGATCAAAAAATTGAAATTATAAATGATAGAATACAAAATCTAAAGGTAAAATGTGATCTTGTTA
CTGTAAGAGCATTAGCAAATTTAAGTACAATATTGTCACATACAAAGCAATTCACTGTTAAAGACAAATATTTAATTTTA
AAAGGTAAGAATTATCAGCATGAGATCACTCAAGCATTATTACATAATAAATTTAACTATACGCTATATCAAAGTTGTAC
TGATGATAGTTCATGGATACTAGAAATTAGAATATAA

Upstream 100 bases:

>100_bases
ATAATGTTTCACGTGAAACATTATTTATAATATGATATTCATTATAATTTCTAGAAAATAACTTTGCCATTAATTAATAG
ATAATTTTAGTAACATAAAT

Downstream 100 bases:

>100_bases
CAATCAATGAGTTACAACGCACAAATTATAGTTATTGTTAATCAAAAAGGTGGTGTAGGTAAAACTACTACTGCTACTAA
CTTAGCTACAGCATTTGCTG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MLQQIYDFLNINYNVSRETFNKFKEYYSLLSKWNSTINLVSATTLQNFWQRHIFDSIQLLNYIHNKDIIVADLGSGAGFP
GVVLSISGIKNVILIESDSRKAAFLLQAAQLSDQKIEIINDRIQNLKVKCDLVTVRALANLSTILSHTKQFTVKDKYLIL
KGKNYQHEITQALLHNKFNYTLYQSCTDDSSWILEIRI

Sequences:

>Translated_198_residues
MLQQIYDFLNINYNVSRETFNKFKEYYSLLSKWNSTINLVSATTLQNFWQRHIFDSIQLLNYIHNKDIIVADLGSGAGFP
GVVLSISGIKNVILIESDSRKAAFLLQAAQLSDQKIEIINDRIQNLKVKCDLVTVRALANLSTILSHTKQFTVKDKYLIL
KGKNYQHEITQALLHNKFNYTLYQSCTDDSSWILEIRI
>Mature_198_residues
MLQQIYDFLNINYNVSRETFNKFKEYYSLLSKWNSTINLVSATTLQNFWQRHIFDSIQLLNYIHNKDIIVADLGSGAGFP
GVVLSISGIKNVILIESDSRKAAFLLQAAQLSDQKIEIINDRIQNLKVKCDLVTVRALANLSTILSHTKQFTVKDKYLIL
KGKNYQHEITQALLHNKFNYTLYQSCTDDSSWILEIRI

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=163, Percent_Identity=30.6748466257669, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_ORITB (A5CDU7)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248672.1
- ProteinModelPortal:   A5CDU7
- SMR:   A5CDU7
- STRING:   A5CDU7
- GeneID:   5219627
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_1039
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   KPLLEIC
- ProtClustDB:   PRK00107
- BioCyc:   OTSU357244:OTBS_1039-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 22838; Mature: 22838

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQQIYDFLNINYNVSRETFNKFKEYYSLLSKWNSTINLVSATTLQNFWQRHIFDSIQLL
CHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHH
NYIHNKDIIVADLGSGAGFPGVVLSISGIKNVILIESDSRKAAFLLQAAQLSDQKIEIIN
HHHCCCCEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHH
DRIQNLKVKCDLVTVRALANLSTILSHTKQFTVKDKYLILKGKNYQHEITQALLHNKFNY
HHHHCEEEEEEEEHHHHHHHHHHHHHHHHCEEEECEEEEEECCCCHHHHHHHHHHCCCCE
TLYQSCTDDSSWILEIRI
EEEECCCCCCCEEEEEEC
>Mature Secondary Structure
MLQQIYDFLNINYNVSRETFNKFKEYYSLLSKWNSTINLVSATTLQNFWQRHIFDSIQLL
CHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHH
NYIHNKDIIVADLGSGAGFPGVVLSISGIKNVILIESDSRKAAFLLQAAQLSDQKIEIIN
HHHCCCCEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHH
DRIQNLKVKCDLVTVRALANLSTILSHTKQFTVKDKYLILKGKNYQHEITQALLHNKFNY
HHHHCEEEEEEEEHHHHHHHHHHHHHHHHCEEEECEEEEEECCCCHHHHHHHHHHCCCCE
TLYQSCTDDSSWILEIRI
EEEECCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA