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The map label for this gene is rho [H]

Identifier: 148284565

GI number: 148284565

Start: 997544

End: 999049

Strand: Direct

Name: rho [H]

Synonym: OTBS_1012

Alternate gene names: 148284565

Gene position: 997544-999049 (Clockwise)

Preceding gene: 148284559

Following gene: 148284566

Centisome position: 46.9

GC content: 32.8

Gene sequence:

>1506_bases
ATGACTACAGAAAAAGAACATAGAATAAAAAACATATTAAAACCTGTATCTAATAATAATCCAGCCAACAGCGAAGCTGT
AGATTCTTCAGTTGAAATAACTGCTCATTTGAACGAACCAAAGAAAAAATCTCCTGAAGCCTTGCAATGTTATGCTGAAA
GTAAAGAACATAGAATAAAAAAACCATCCAAACCTGTATCTAACAATAATCAAGTTAACATCGAAGCTGTAGATTCTTCA
GTTGAAATAACTGTTCATTTGAACGAACTAAAGAAAAAATCTCCTGAAGCATTGCAATGTTATGCTGAAAGTTTAGCAAT
TGAAAATGCTAGTTCATTAACTAAGAATGAACTAATATCAGCTATTTTAAAAATGGTAGTAGAACGTAATGGTACAGTTA
TTGAAGAAGGAGTAATTGAAATTTTAAACGGAGGATTTGGTTTTTTACGATCACCAGAAGTAAATTATTTATCAGGAGCA
GGAGATATTTATGTATCTTTAAGTCAGATTAAACGTTGTGAATTACGCACTGGAGATACTGTTGAAGGACAAATTAGAGC
TCCAAAGTCTGGAGAACGTTACTTTGCTTTATTAAAAGTACTAAAAGTTAATGGAGAAGATATTAAAAAATCTATTCAAC
GGCTACATTTTGATGATTTAGTAGCTTTATACCCAGAGGAAAAAATTTTTTTTGAAGTTGATCTAGATTATAAAAACCTT
AGTACTAGAGTTATGGAACTAGTTTCACCTATGGGGAAAGGGCAACGAGCTCTTATAGTCGCTCCGCCTCGTACTGGTAA
AACAATTTTATTGCAGAACATTGCTCATTCTATTGCTACTAATCATCCTGAAATTTACCTAATAGTATTGCTAATAGGAG
AAAGGCCAGAAGAAGTTACCGATATGATTAGGTCAGTTAAAGGAGAAGTTGTAAGTTCTACTTTTGATGAGCCAGCAGTA
CGGCATACTCAACTAGCTGAAATTGTAATTGAAAAAGCAAAAAGATTGGTAGAGCATAAAAGAGATGTAGTAATATTACT
GGATTCAATCACTAGACTAGCTAGGGCATACAATACTGTTGTTCCATCTTCAGGTAAGGTACTAACTGGTGGAGTTGATT
CAAACGCTTTACAAAAACCAAAAAGCTTTTTTGGAGCAGCTCGTAATATCGAAAATGGTGGGTCTTTAACTATTATTGCA
ACTGCATTAGTTGATACAGGTTCTAGAATGGATGAAGTGATTTTTGAGGAGTTTAAAGGAACTGGCAACTCAGAAATTGT
ATTAGACAGAAAGATATCTGATAAAAGAATCTATCCAGCAATTGATATTAATAAATCTGGCACTAGAAAAGAGGAGCTGT
TAGTTGGTAAATCAGAATTATCAAAAATGTGGATGCTTAGAAGAATTATTAATCAAATGGGAGCTGTGGACGCTATGGAA
TTTTTACTTGATAAACTTAAAAACACTAAATCTAATAGTGAATTCTTTGATTTGATGAATTCATAG

Upstream 100 bases:

>100_bases
TTAAGTTTACTATACACCTTAATTATCAGTTTAATTAATATTTCATACTAGAAAGCTAATGCATTTATATCAAACTCAAT
ATAATCTTTAATAATATATT

Downstream 100 bases:

>100_bases
AAACGTAAGATTAGGATAAGTAAAGATAAGAAAAAGAATAGGATATAAGATTGGGATTAAGTTTAGATATATAATAACTT
ACAACAGAAGATATTATATG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MTTEKEHRIKNILKPVSNNNPANSEAVDSSVEITAHLNEPKKKSPEALQCYAESKEHRIKKPSKPVSNNNQVNIEAVDSS
VEITVHLNELKKKSPEALQCYAESLAIENASSLTKNELISAILKMVVERNGTVIEEGVIEILNGGFGFLRSPEVNYLSGA
GDIYVSLSQIKRCELRTGDTVEGQIRAPKSGERYFALLKVLKVNGEDIKKSIQRLHFDDLVALYPEEKIFFEVDLDYKNL
STRVMELVSPMGKGQRALIVAPPRTGKTILLQNIAHSIATNHPEIYLIVLLIGERPEEVTDMIRSVKGEVVSSTFDEPAV
RHTQLAEIVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVPSSGKVLTGGVDSNALQKPKSFFGAARNIENGGSLTIIA
TALVDTGSRMDEVIFEEFKGTGNSEIVLDRKISDKRIYPAIDINKSGTRKEELLVGKSELSKMWMLRRIINQMGAVDAME
FLLDKLKNTKSNSEFFDLMNS

Sequences:

>Translated_501_residues
MTTEKEHRIKNILKPVSNNNPANSEAVDSSVEITAHLNEPKKKSPEALQCYAESKEHRIKKPSKPVSNNNQVNIEAVDSS
VEITVHLNELKKKSPEALQCYAESLAIENASSLTKNELISAILKMVVERNGTVIEEGVIEILNGGFGFLRSPEVNYLSGA
GDIYVSLSQIKRCELRTGDTVEGQIRAPKSGERYFALLKVLKVNGEDIKKSIQRLHFDDLVALYPEEKIFFEVDLDYKNL
STRVMELVSPMGKGQRALIVAPPRTGKTILLQNIAHSIATNHPEIYLIVLLIGERPEEVTDMIRSVKGEVVSSTFDEPAV
RHTQLAEIVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVPSSGKVLTGGVDSNALQKPKSFFGAARNIENGGSLTIIA
TALVDTGSRMDEVIFEEFKGTGNSEIVLDRKISDKRIYPAIDINKSGTRKEELLVGKSELSKMWMLRRIINQMGAVDAME
FLLDKLKNTKSNSEFFDLMNS
>Mature_500_residues
TTEKEHRIKNILKPVSNNNPANSEAVDSSVEITAHLNEPKKKSPEALQCYAESKEHRIKKPSKPVSNNNQVNIEAVDSSV
EITVHLNELKKKSPEALQCYAESLAIENASSLTKNELISAILKMVVERNGTVIEEGVIEILNGGFGFLRSPEVNYLSGAG
DIYVSLSQIKRCELRTGDTVEGQIRAPKSGERYFALLKVLKVNGEDIKKSIQRLHFDDLVALYPEEKIFFEVDLDYKNLS
TRVMELVSPMGKGQRALIVAPPRTGKTILLQNIAHSIATNHPEIYLIVLLIGERPEEVTDMIRSVKGEVVSSTFDEPAVR
HTQLAEIVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVPSSGKVLTGGVDSNALQKPKSFFGAARNIENGGSLTIIAT
ALVDTGSRMDEVIFEEFKGTGNSEIVLDRKISDKRIYPAIDINKSGTRKEELLVGKSELSKMWMLRRIINQMGAVDAMEF
LLDKLKNTKSNSEFFDLMNS

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=61.7788461538462, Blast_Score=524, Evalue=1e-150,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 55698; Mature: 55567

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEKEHRIKNILKPVSNNNPANSEAVDSSVEITAHLNEPKKKSPEALQCYAESKEHRIK
CCCCHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
KPSKPVSNNNQVNIEAVDSSVEITVHLNELKKKSPEALQCYAESLAIENASSLTKNELIS
CCCCCCCCCCEEEEEEECCEEEEEEEEHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
AILKMVVERNGTVIEEGVIEILNGGFGFLRSPEVNYLSGAGDIYVSLSQIKRCELRTGDT
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEEHHHHHHHHCCCCCC
VEGQIRAPKSGERYFALLKVLKVNGEDIKKSIQRLHFDDLVALYPEEKIFFEVDLDYKNL
CCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHCCCCCEEEEEEECCHHHH
STRVMELVSPMGKGQRALIVAPPRTGKTILLQNIAHSIATNHPEIYLIVLLIGERPEEVT
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHH
DMIRSVKGEVVSSTFDEPAVRHTQLAEIVIEKAKRLVEHKRDVVILLDSITRLARAYNTV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHC
VPSSGKVLTGGVDSNALQKPKSFFGAARNIENGGSLTIIATALVDTGSRMDEVIFEEFKG
CCCCCCEEECCCCCHHHHCHHHHHHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCC
TGNSEIVLDRKISDKRIYPAIDINKSGTRKEELLVGKSELSKMWMLRRIINQMGAVDAME
CCCCEEEEECCCCCCCCCCEEECCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHCHHHHHH
FLLDKLKNTKSNSEFFDLMNS
HHHHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure 
TTEKEHRIKNILKPVSNNNPANSEAVDSSVEITAHLNEPKKKSPEALQCYAESKEHRIK
CCCHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
KPSKPVSNNNQVNIEAVDSSVEITVHLNELKKKSPEALQCYAESLAIENASSLTKNELIS
CCCCCCCCCCEEEEEEECCEEEEEEEEHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
AILKMVVERNGTVIEEGVIEILNGGFGFLRSPEVNYLSGAGDIYVSLSQIKRCELRTGDT
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEEHHHHHHHHCCCCCC
VEGQIRAPKSGERYFALLKVLKVNGEDIKKSIQRLHFDDLVALYPEEKIFFEVDLDYKNL
CCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHCCCCCEEEEEEECCHHHH
STRVMELVSPMGKGQRALIVAPPRTGKTILLQNIAHSIATNHPEIYLIVLLIGERPEEVT
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHH
DMIRSVKGEVVSSTFDEPAVRHTQLAEIVIEKAKRLVEHKRDVVILLDSITRLARAYNTV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHC
VPSSGKVLTGGVDSNALQKPKSFFGAARNIENGGSLTIIATALVDTGSRMDEVIFEEFKG
CCCCCCEEECCCCCHHHHCHHHHHHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCC
TGNSEIVLDRKISDKRIYPAIDINKSGTRKEELLVGKSELSKMWMLRRIINQMGAVDAME
CCCCEEEEECCCCCCCCCCEEECCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHCHHHHHH
FLLDKLKNTKSNSEFFDLMNS
HHHHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]