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The map label for this gene is ppdK [H]

Identifier: 148284437

GI number: 148284437

Start: 743709

End: 746414

Strand: Direct

Name: ppdK [H]

Synonym: OTBS_0767

Alternate gene names: 148284437

Gene position: 743709-746414 (Clockwise)

Preceding gene: 148284436

Following gene: 148284439

Centisome position: 34.96

GC content: 31.93

Gene sequence:

>2706_bases
ATGACAAAGTGGATTTATCGATTTTCAAGTATGTCTGCAAACTTACCTCATATTAATTCAGTAGGCCAATCCTGTAATAT
AATAGAGGATAATCTGCAAAATCTACTTGGTAACAAAGGTATAACACTTGCTCAGATGTTTAGTCAAAAGCTACCTGTAC
CGCATGGTTTTATTATATCAACTAAATTATATCGTTACTATCGGCAAAATAATGATTCTCTACCAAGTGATTTCATATCT
GATTTAAAATCATCTATTCTAGATTTAGAACATCAAACTGGAAAAAATTTTGGTGATTATAATAATCCGCTATTGGTTGC
AGTAAGGTCTAGTGCTGCAGTATCTATGCCTGGAATTATGAACACTATACTTAATCTAGGTATAAATGATATTATAGTTG
AAGGATTAAGTACTGCAACAGGTAATAGAATTTTTGCATTAGATACTTATGTGCGCTTTATTCAAACTTATGGACAAGTA
GTTATGGGAGTATCACCCGCAGAATTTGAACTTGCTTTAAATCAGTTTAAAACATTAAATAATATTACTAGTCAAGAGCA
GCTAACTCCAGAACATTTGCATAATCTAATTACAATTTTTAAGCAAATTATTCATAAAGAAACTAAACAAGAATTTCCAC
AAGATGTTAATATTCAATTACACCAAGCTATTCAAGCAGTATTAAAATCATGGATGAACCACCATGCTATTACGTATAGA
AAACTTAACAATATTTCAGAAAATATTGGAACTGCTATTATTGTACAAACTATGGTTTTTGGTAATATGGGTAGTGATTC
AGCAACAGGAGTAGTGTTTACTAGAAATCCTTTAACAGGTGATAATAAACTATATGGCGAATTTTTAATTAATGCTCAAG
GAGATAACGTTGTATCTGGAGCTAGTATACCTAATTCCATATTAAGTGATAATAAGGATCAAAAATGCACCTTAAAGTAT
GCTATGCCTAAGATGTATGAAGAGTTATTACAGCATTGCAAACAATTGGAACAGCATTACTGTAATATTCAAGATATTGA
ATTTACTATAGAAAAGCAGAAATTATATATATTACAGACAAGATCCGCAAAATGCACAGCATTAGCAACTATTAAGGCAC
TAGTAGATATGGTTGCCTCTGGGTTAATAACTAAAAAAGATGCGATAGCTAAAATCAATCCAATGTCACTCAATCAATTA
CTACACTCATATATTGATGAAAGTGAGAATTATAAAGTAATAACTCAAGGACTTCCGTCTACATCAGGAGCTGTAACTGG
AGTAATTGCATTATCAGTAGCTGCTGCTGAAAAATTATCTAATCAACATCATAAAGTGATTTTTATATGTTCGAATATTA
GTCCAGAAGACATTAAAGCTATGCATATATCTTCTGGCATTTTAACTACTGGTGGAAATACAACTTCTCATGCAGCAATT
CTTAGCAGAGCATTAGGAAAGCCATATATTTGCGCTGCTAAAGATGTTATAATTGACCAACAGAATGGACTGATAAAGAT
AGGCGATATTAGTTTGTATCAAGGCAGTAAAATTACTATAGATGGTAACAGTGGTAAAGTATTTGTAGGAGAAGTAAGAT
TACTACAGCCAGAATTTTCTAAGGAATGCCAATTATTTATGGAGTGGGCTGATGAAATAAAAACAGTTGAGGTAAAGGCT
AATGCAGATACTAGCGATGATGTAAAATTAGCACTGCGACTTGGGGCAACATCTATCGGTCTTTGCCGTACTGAGCATAT
GCTTCTTGAGGACAACAAAATTAGCCTAGTTCAGGAGACAATTATTGCTGATAATGATAAATGTAGATTGAATGCGCTTG
AAAAATTGTTTTTGTTGCATAAAGCTGATTTTAAAGAGCTATTTGCTGCTACAATGCAAACCCCAATTAATATCAGGCTA
CTTGACGCACCATTACATGAATTTCTACCTACAACATTTACAACTGCACACAATAGAATCATAGATAATCTAAACATTTC
TCAAGAAACGTTTAATGCAAGAATTAACACGTTAAAGGAAGTAAATCCTATGTTAGGTCATAGAGGTTGTAGAATCGGTA
TAAGTTTCCCTGAAATTTATGAAATGCAGGTTCAAGCAATATTTGAAGCTGCAGCTGAAATATTACTTGAGTTAAAAGTT
ATGCCTAAAATTGAAGTTACTATACCGTTAATTAGCCATAAAAAAGAGTTGCAATTGTTAAAGCAAATAATTTATAAAAT
AGTAGAAAAAGTAGAACAGCAAAAATTACTTAAATATAGATGTAAGTTTGATTATACAATAGGAACTATGATTGAATTGC
CTAGAGCTGCTCTTCAGGCATATGAACTGGCTCAGGAAGTGGATTTTTTCTGTTTTGGTACTAACGATTTAACGCAAACT
ACATATGGTATTTCAAGAGATGATTCTACATCTTTTATTAATCAATATATTGAAAATGATATTTTTGCAGCTGATCCTTT
TGCAACTTTAGATTGCGAAGCTGTAGGTGAATTAATTCAAATAGCCATTGAACGTGGCAGAACTGCTAAAAAACATCTGA
AAATAGGAATTTGTGGTGAACATGCTGCTGATTTAAATTCAGTAGAATTTTTTCATAAAGTTAAAGTAGATTCAATATCA
TGTTCGCCATATAAAATTCCAATTGCTAGATTAGCAGCAGCACAAGCAGCATTAAAAAATAATTAA

Upstream 100 bases:

>100_bases
TTAAATTATATAAGCATAAAATTAATAACTTTGAAAATAACATGACCTAACTTAATAATTAGTTTATGCAAATACTATAC
AATCATTTAGAGGCAATATT

Downstream 100 bases:

>100_bases
CTACGAGTTAGATATAAGAAATAATGTAAAAAATCTAGAAGAATAAATTTTAATGCTTATAGCTTAATTCGAGAAGGTAG
TTGCACATTGTAAAGATAAA

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 901; Mature: 900

Protein sequence:

>901_residues
MTKWIYRFSSMSANLPHINSVGQSCNIIEDNLQNLLGNKGITLAQMFSQKLPVPHGFIISTKLYRYYRQNNDSLPSDFIS
DLKSSILDLEHQTGKNFGDYNNPLLVAVRSSAAVSMPGIMNTILNLGINDIIVEGLSTATGNRIFALDTYVRFIQTYGQV
VMGVSPAEFELALNQFKTLNNITSQEQLTPEHLHNLITIFKQIIHKETKQEFPQDVNIQLHQAIQAVLKSWMNHHAITYR
KLNNISENIGTAIIVQTMVFGNMGSDSATGVVFTRNPLTGDNKLYGEFLINAQGDNVVSGASIPNSILSDNKDQKCTLKY
AMPKMYEELLQHCKQLEQHYCNIQDIEFTIEKQKLYILQTRSAKCTALATIKALVDMVASGLITKKDAIAKINPMSLNQL
LHSYIDESENYKVITQGLPSTSGAVTGVIALSVAAAEKLSNQHHKVIFICSNISPEDIKAMHISSGILTTGGNTTSHAAI
LSRALGKPYICAAKDVIIDQQNGLIKIGDISLYQGSKITIDGNSGKVFVGEVRLLQPEFSKECQLFMEWADEIKTVEVKA
NADTSDDVKLALRLGATSIGLCRTEHMLLEDNKISLVQETIIADNDKCRLNALEKLFLLHKADFKELFAATMQTPINIRL
LDAPLHEFLPTTFTTAHNRIIDNLNISQETFNARINTLKEVNPMLGHRGCRIGISFPEIYEMQVQAIFEAAAEILLELKV
MPKIEVTIPLISHKKELQLLKQIIYKIVEKVEQQKLLKYRCKFDYTIGTMIELPRAALQAYELAQEVDFFCFGTNDLTQT
TYGISRDDSTSFINQYIENDIFAADPFATLDCEAVGELIQIAIERGRTAKKHLKIGICGEHAADLNSVEFFHKVKVDSIS
CSPYKIPIARLAAAQAALKNN

Sequences:

>Translated_901_residues
MTKWIYRFSSMSANLPHINSVGQSCNIIEDNLQNLLGNKGITLAQMFSQKLPVPHGFIISTKLYRYYRQNNDSLPSDFIS
DLKSSILDLEHQTGKNFGDYNNPLLVAVRSSAAVSMPGIMNTILNLGINDIIVEGLSTATGNRIFALDTYVRFIQTYGQV
VMGVSPAEFELALNQFKTLNNITSQEQLTPEHLHNLITIFKQIIHKETKQEFPQDVNIQLHQAIQAVLKSWMNHHAITYR
KLNNISENIGTAIIVQTMVFGNMGSDSATGVVFTRNPLTGDNKLYGEFLINAQGDNVVSGASIPNSILSDNKDQKCTLKY
AMPKMYEELLQHCKQLEQHYCNIQDIEFTIEKQKLYILQTRSAKCTALATIKALVDMVASGLITKKDAIAKINPMSLNQL
LHSYIDESENYKVITQGLPSTSGAVTGVIALSVAAAEKLSNQHHKVIFICSNISPEDIKAMHISSGILTTGGNTTSHAAI
LSRALGKPYICAAKDVIIDQQNGLIKIGDISLYQGSKITIDGNSGKVFVGEVRLLQPEFSKECQLFMEWADEIKTVEVKA
NADTSDDVKLALRLGATSIGLCRTEHMLLEDNKISLVQETIIADNDKCRLNALEKLFLLHKADFKELFAATMQTPINIRL
LDAPLHEFLPTTFTTAHNRIIDNLNISQETFNARINTLKEVNPMLGHRGCRIGISFPEIYEMQVQAIFEAAAEILLELKV
MPKIEVTIPLISHKKELQLLKQIIYKIVEKVEQQKLLKYRCKFDYTIGTMIELPRAALQAYELAQEVDFFCFGTNDLTQT
TYGISRDDSTSFINQYIENDIFAADPFATLDCEAVGELIQIAIERGRTAKKHLKIGICGEHAADLNSVEFFHKVKVDSIS
CSPYKIPIARLAAAQAALKNN
>Mature_900_residues
TKWIYRFSSMSANLPHINSVGQSCNIIEDNLQNLLGNKGITLAQMFSQKLPVPHGFIISTKLYRYYRQNNDSLPSDFISD
LKSSILDLEHQTGKNFGDYNNPLLVAVRSSAAVSMPGIMNTILNLGINDIIVEGLSTATGNRIFALDTYVRFIQTYGQVV
MGVSPAEFELALNQFKTLNNITSQEQLTPEHLHNLITIFKQIIHKETKQEFPQDVNIQLHQAIQAVLKSWMNHHAITYRK
LNNISENIGTAIIVQTMVFGNMGSDSATGVVFTRNPLTGDNKLYGEFLINAQGDNVVSGASIPNSILSDNKDQKCTLKYA
MPKMYEELLQHCKQLEQHYCNIQDIEFTIEKQKLYILQTRSAKCTALATIKALVDMVASGLITKKDAIAKINPMSLNQLL
HSYIDESENYKVITQGLPSTSGAVTGVIALSVAAAEKLSNQHHKVIFICSNISPEDIKAMHISSGILTTGGNTTSHAAIL
SRALGKPYICAAKDVIIDQQNGLIKIGDISLYQGSKITIDGNSGKVFVGEVRLLQPEFSKECQLFMEWADEIKTVEVKAN
ADTSDDVKLALRLGATSIGLCRTEHMLLEDNKISLVQETIIADNDKCRLNALEKLFLLHKADFKELFAATMQTPINIRLL
DAPLHEFLPTTFTTAHNRIIDNLNISQETFNARINTLKEVNPMLGHRGCRIGISFPEIYEMQVQAIFEAAAEILLELKVM
PKIEVTIPLISHKKELQLLKQIIYKIVEKVEQQKLLKYRCKFDYTIGTMIELPRAALQAYELAQEVDFFCFGTNDLTQTT
YGISRDDSTSFINQYIENDIFAADPFATLDCEAVGELIQIAIERGRTAKKHLKIGICGEHAADLNSVEFFHKVKVDSISC
SPYKIPIARLAAAQAALKNN

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=460, Percent_Identity=25.6521739130435, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI48994992, Length=446, Percent_Identity=25.7847533632287, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1788726, Length=640, Percent_Identity=20.15625, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1789193, Length=413, Percent_Identity=24.6973365617433, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 100387; Mature: 100256

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKWIYRFSSMSANLPHINSVGQSCNIIEDNLQNLLGNKGITLAQMFSQKLPVPHGFIIS
CCCCEEEECCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEHH
TKLYRYYRQNNDSLPSDFISDLKSSILDLEHQTGKNFGDYNNPLLVAVRSSAAVSMPGIM
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHH
NTILNLGINDIIVEGLSTATGNRIFALDTYVRFIQTYGQVVMGVSPAEFELALNQFKTLN
HHHHHCCHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
NITSQEQLTPEHLHNLITIFKQIIHKETKQEFPQDVNIQLHQAIQAVLKSWMNHHAITYR
HCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEHH
KLNNISENIGTAIIVQTMVFGNMGSDSATGVVFTRNPLTGDNKLYGEFLINAQGDNVVSG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCEEEC
ASIPNSILSDNKDQKCTLKYAMPKMYEELLQHCKQLEQHYCNIQDIEFTIEKQKLYILQT
CCCCHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEEE
RSAKCTALATIKALVDMVASGLITKKDAIAKINPMSLNQLLHSYIDESENYKVITQGLPS
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCC
TSGAVTGVIALSVAAAEKLSNQHHKVIFICSNISPEDIKAMHISSGILTTGGNTTSHAAI
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHEEEEECCCEEECCCCCHHHHHH
LSRALGKPYICAAKDVIIDQQNGLIKIGDISLYQGSKITIDGNSGKVFVGEVRLLQPEFS
HHHHCCCCEEEECCEEEEECCCCEEEEECEEEECCCEEEEECCCCEEEEEEEEEECCCHH
KECQLFMEWADEIKTVEVKANADTSDDVKLALRLGATSIGLCRTEHMLLEDNKISLVQET
HHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEECCCCCCHHCCCCEEECCCCHHHHHHH
IIADNDKCRLNALEKLFLLHKADFKELFAATMQTPINIRLLDAPLHEFLPTTFTTAHNRI
HHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHHH
IDNLNISQETFNARINTLKEVNPMLGHRGCRIGISFPEIYEMQVQAIFEAAAEILLELKV
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
MPKIEVTIPLISHKKELQLLKQIIYKIVEKVEQQKLLKYRCKFDYTIGTMIELPRAALQA
CCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YELAQEVDFFCFGTNDLTQTTYGISRDDSTSFINQYIENDIFAADPFATLDCEAVGELIQ
HHHHHHCCEEEECCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
IAIERGRTAKKHLKIGICGEHAADLNSVEFFHKVKVDSISCSPYKIPIARLAAAQAALKN
HHHHCCCCCHHHEEEEECCCCCCCCCHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHCC
N
C
>Mature Secondary Structure 
TKWIYRFSSMSANLPHINSVGQSCNIIEDNLQNLLGNKGITLAQMFSQKLPVPHGFIIS
CCCEEEECCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEHH
TKLYRYYRQNNDSLPSDFISDLKSSILDLEHQTGKNFGDYNNPLLVAVRSSAAVSMPGIM
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHH
NTILNLGINDIIVEGLSTATGNRIFALDTYVRFIQTYGQVVMGVSPAEFELALNQFKTLN
HHHHHCCHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
NITSQEQLTPEHLHNLITIFKQIIHKETKQEFPQDVNIQLHQAIQAVLKSWMNHHAITYR
HCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEHH
KLNNISENIGTAIIVQTMVFGNMGSDSATGVVFTRNPLTGDNKLYGEFLINAQGDNVVSG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCEEEC
ASIPNSILSDNKDQKCTLKYAMPKMYEELLQHCKQLEQHYCNIQDIEFTIEKQKLYILQT
CCCCHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEEE
RSAKCTALATIKALVDMVASGLITKKDAIAKINPMSLNQLLHSYIDESENYKVITQGLPS
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCC
TSGAVTGVIALSVAAAEKLSNQHHKVIFICSNISPEDIKAMHISSGILTTGGNTTSHAAI
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHEEEEECCCEEECCCCCHHHHHH
LSRALGKPYICAAKDVIIDQQNGLIKIGDISLYQGSKITIDGNSGKVFVGEVRLLQPEFS
HHHHCCCCEEEECCEEEEECCCCEEEEECEEEECCCEEEEECCCCEEEEEEEEEECCCHH
KECQLFMEWADEIKTVEVKANADTSDDVKLALRLGATSIGLCRTEHMLLEDNKISLVQET
HHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEECCCCCCHHCCCCEEECCCCHHHHHHH
IIADNDKCRLNALEKLFLLHKADFKELFAATMQTPINIRLLDAPLHEFLPTTFTTAHNRI
HHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHHH
IDNLNISQETFNARINTLKEVNPMLGHRGCRIGISFPEIYEMQVQAIFEAAAEILLELKV
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
MPKIEVTIPLISHKKELQLLKQIIYKIVEKVEQQKLLKYRCKFDYTIGTMIELPRAALQA
CCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YELAQEVDFFCFGTNDLTQTTYGISRDDSTSFINQYIENDIFAADPFATLDCEAVGELIQ
HHHHHHCCEEEECCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
IAIERGRTAKKHLKIGICGEHAADLNSVEFFHKVKVDSISCSPYKIPIARLAAAQAALKN
HHHHCCCCCHHHEEEEECCCCCCCCCHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]