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The map label for this gene is rsmE [H]

Identifier: 148284290

GI number: 148284290

Start: 470902

End: 471654

Strand: Direct

Name: rsmE [H]

Synonym: OTBS_0493

Alternate gene names: 148284290

Gene position: 470902-471654 (Clockwise)

Preceding gene: 148284285

Following gene: 148284291

Centisome position: 22.14

GC content: 30.81

Gene sequence:

>753_bases
ATGAAACTTAAAAAATTACACAGGATATATAGCAGTATACCATTGAGTAAAGCTGGTACTATCAAACTCTCAAGAGAGCA
AGCTAACTACATAATCAATGTACTAAGGTGTAAAAACAATGATCAAATTAGAGTATTTAATGAACTAAATGGAGAATATT
TAGCTATTATTCATCATAATAACAACAAAGAAGTATTATTGCAAATTATAAATCAAACTAAAGTAGTTGCAAAAGAAGTT
GATGCAAATAACTCGCATTTAGTTCTTGCTCAGTGTATTATTAAACCAGAAAGAATGGCCAGAGTAATTGATATGGCAAC
TCAAGTAGGAATAACAAACATAGTACCAATCATAAGCGAATATTCTCAACATCTAAAATTTAACACAGAAAAATATAATA
AAATCATCATAGAATCTTGCAGACAATCAGAACGATTAACCATACCTATTCTATCATCTCCAGTAACATTATTGCATTTT
TTAAGCCAAAGTAACTCCGAGTGCATATTATGTGCTAATGAACAGGAAAAAATACAACAAATACATCATATTCCGGCATC
AATACTTTCTAAAGCTTCAATTCTAATTGGGCCAGAGGGCGGTTTCTCTGAACAGGATCAAAGCATATTAAGGAAAGCAG
GAAGATTAATAAAATCGATAAGCATTGGAAGAAATGTTTTAAGATCTGAAACTGCTGCAATATATCTTTTATCGCAGTTA
CAATTATTAAAAATTATGCACGTGCGTGCATAG

Upstream 100 bases:

>100_bases
GCTTGAGCAGATAATAATTTAAAAAAATAAAAATGTCAAATATCGATTGATTAATTTATATTATCAATATTTAATACATT
TCAACATTCCAAATTAGAAT

Downstream 100 bases:

>100_bases
TAATTACGAAGATATTAATATTAGACCTCTTTCGAAACTGGTTAACGTAGTTCAAAATTATAAATGCCAGAGGTCAAATT
AAATCTAAGACCGAATCTTT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKLKKLHRIYSSIPLSKAGTIKLSREQANYIINVLRCKNNDQIRVFNELNGEYLAIIHHNNNKEVLLQIINQTKVVAKEV
DANNSHLVLAQCIIKPERMARVIDMATQVGITNIVPIISEYSQHLKFNTEKYNKIIIESCRQSERLTIPILSSPVTLLHF
LSQSNSECILCANEQEKIQQIHHIPASILSKASILIGPEGGFSEQDQSILRKAGRLIKSISIGRNVLRSETAAIYLLSQL
QLLKIMHVRA

Sequences:

>Translated_250_residues
MKLKKLHRIYSSIPLSKAGTIKLSREQANYIINVLRCKNNDQIRVFNELNGEYLAIIHHNNNKEVLLQIINQTKVVAKEV
DANNSHLVLAQCIIKPERMARVIDMATQVGITNIVPIISEYSQHLKFNTEKYNKIIIESCRQSERLTIPILSSPVTLLHF
LSQSNSECILCANEQEKIQQIHHIPASILSKASILIGPEGGFSEQDQSILRKAGRLIKSISIGRNVLRSETAAIYLLSQL
QLLKIMHVRA
>Mature_250_residues
MKLKKLHRIYSSIPLSKAGTIKLSREQANYIINVLRCKNNDQIRVFNELNGEYLAIIHHNNNKEVLLQIINQTKVVAKEV
DANNSHLVLAQCIIKPERMARVIDMATQVGITNIVPIISEYSQHLKFNTEKYNKIIIESCRQSERLTIPILSSPVTLLHF
LSQSNSECILCANEQEKIQQIHHIPASILSKASILIGPEGGFSEQDQSILRKAGRLIKSISIGRNVLRSETAAIYLLSQL
QLLKIMHVRA

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=254, Percent_Identity=27.5590551181102, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 28261; Mature: 28261

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKKLHRIYSSIPLSKAGTIKLSREQANYIINVLRCKNNDQIRVFNELNGEYLAIIHHN
CCHHHHHHHHHHCCCCCCCEEEEEHHHHHHEEEEEEECCCCCEEEEEECCCCEEEEEEEC
NNKEVLLQIINQTKVVAKEVDANNSHLVLAQCIIKPERMARVIDMATQVGITNIVPIISE
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHH
YSQHLKFNTEKYNKIIIESCRQSERLTIPILSSPVTLLHFLSQSNSECILCANEQEKIQQ
HHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCEEEECCCHHHHHH
IHHIPASILSKASILIGPEGGFSEQDQSILRKAGRLIKSISIGRNVLRSETAAIYLLSQL
HHHCHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLLKIMHVRA
HHHHHHHHCC
>Mature Secondary Structure
MKLKKLHRIYSSIPLSKAGTIKLSREQANYIINVLRCKNNDQIRVFNELNGEYLAIIHHN
CCHHHHHHHHHHCCCCCCCEEEEEHHHHHHEEEEEEECCCCCEEEEEECCCCEEEEEEEC
NNKEVLLQIINQTKVVAKEVDANNSHLVLAQCIIKPERMARVIDMATQVGITNIVPIISE
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHH
YSQHLKFNTEKYNKIIIESCRQSERLTIPILSSPVTLLHFLSQSNSECILCANEQEKIQQ
HHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCEEEECCCHHHHHH
IHHIPASILSKASILIGPEGGFSEQDQSILRKAGRLIKSISIGRNVLRSETAAIYLLSQL
HHHCHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLLKIMHVRA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]