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The map label for this gene is spoU [H]

Identifier: 148284217

GI number: 148284217

Start: 361910

End: 362638

Strand: Direct

Name: spoU [H]

Synonym: OTBS_0379

Alternate gene names: 148284217

Gene position: 361910-362638 (Clockwise)

Preceding gene: 148284186

Following gene: 148284218

Centisome position: 17.01

GC content: 30.18

Gene sequence:

>729_bases
ATGACTGATAAAAAATCATATTATATATTTGGTACTCATGCATCCTTAGCAGCTTTAAATAACCCTTTCAGGGTAATAAG
GTATATTTATTGTACAGAGTCATGTTTTCTATCTTTATCAAAATTGATTCAAAAGCATAATTATAAGATAGTTACAACAA
AATTTCTACATCAGCTATTAGGCAGCCAAATAGTTCATCAAGGAATAGCAGTAAATACTATCCCCCTCACAATTAATGGT
ATCAAAAATATAGACCTAAACACACCAAACTATAAAATTGCTATTTTAGACCAATTGCTAGACCATAATAACTTAGGCGC
TATTATAAGATCAGCTGCAGCATTTGATATATCAACTATTATCCTACCTGAAAATAACTCTATAGAAGAGAATGGCACTA
TTGCTAAGATAGCAGTTGGAGCCCTAGAAAAAATAAACTTAGTAAAAGTAAAAAATTTATATAATACTATCAATTACTTA
AAAAAGCATGAATTTTGGATTATCGGGCTAGATCTTAACGATAGTAACCAACTTACAGAGCAAACCTTCCATTCAAAAAC
AGCTTTTGTTTTAGGCTCTGAGAATAAAGGACTAAGGAACCTAACACGTCAACAATGTGATTTTTTAATCAAAATTCCAA
TGTCTCATAAAACTGAAAGTTTAAACGTTGCAGGAGCGGCTACAATAATTTTTTATGAAAGCTTCAAATTTTCCATAAAA
CAACAATAA

Upstream 100 bases:

>100_bases
ATGATTTAGTACTTTCTATAATAATTACTTGATTCTGTCAAGTGATTATTTAATAATCGTCAATTATTACAAAATATTTA
TTTTGTCCAATAAATTTATT

Downstream 100 bases:

>100_bases
TTAAATACCAAGAAAATAATTTTAATAATTCAATAAATACTATTATAATTCTACTAACACTAAAATTTATTATTTTTAAA
AATCAGGAAATATCATGTAT

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MTDKKSYYIFGTHASLAALNNPFRVIRYIYCTESCFLSLSKLIQKHNYKIVTTKFLHQLLGSQIVHQGIAVNTIPLTING
IKNIDLNTPNYKIAILDQLLDHNNLGAIIRSAAAFDISTIILPENNSIEENGTIAKIAVGALEKINLVKVKNLYNTINYL
KKHEFWIIGLDLNDSNQLTEQTFHSKTAFVLGSENKGLRNLTRQQCDFLIKIPMSHKTESLNVAGAATIIFYESFKFSIK
QQ

Sequences:

>Translated_242_residues
MTDKKSYYIFGTHASLAALNNPFRVIRYIYCTESCFLSLSKLIQKHNYKIVTTKFLHQLLGSQIVHQGIAVNTIPLTING
IKNIDLNTPNYKIAILDQLLDHNNLGAIIRSAAAFDISTIILPENNSIEENGTIAKIAVGALEKINLVKVKNLYNTINYL
KKHEFWIIGLDLNDSNQLTEQTFHSKTAFVLGSENKGLRNLTRQQCDFLIKIPMSHKTESLNVAGAATIIFYESFKFSIK
QQ
>Mature_241_residues
TDKKSYYIFGTHASLAALNNPFRVIRYIYCTESCFLSLSKLIQKHNYKIVTTKFLHQLLGSQIVHQGIAVNTIPLTINGI
KNIDLNTPNYKIAILDQLLDHNNLGAIIRSAAAFDISTIILPENNSIEENGTIAKIAVGALEKINLVKVKNLYNTINYLK
KHEFWIIGLDLNDSNQLTEQTFHSKTAFVLGSENKGLRNLTRQQCDFLIKIPMSHKTESLNVAGAATIIFYESFKFSIKQ
Q

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=175, Percent_Identity=35.4285714285714, Blast_Score=120, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27289; Mature: 27158

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKSYYIFGTHASLAALNNPFRVIRYIYCTESCFLSLSKLIQKHNYKIVTTKFLHQLL
CCCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
GSQIVHQGIAVNTIPLTINGIKNIDLNTPNYKIAILDQLLDHNNLGAIIRSAAAFDISTI
HHHHHHCCEEEEEEEEEEECEEEEECCCCCEEEEEEHHHHCCCCHHHHHHHHHHEEEEEE
ILPENNSIEENGTIAKIAVGALEKINLVKVKNLYNTINYLKKHEFWIIGLDLNDSNQLTE
EEECCCCCCCCCCEEEEEHHHHHHCEEEEHHHHHHHHHHHHCCCEEEEEEEECCCCHHHH
QTFHSKTAFVLGSENKGLRNLTRQQCDFLIKIPMSHKTESLNVAGAATIIFYESFKFSIK
HHHCCCEEEEEECCCCCHHHHHHHHCCEEEECCCCCCCCCEECCCEEEEEEEEEEEEEEE
QQ
CC
>Mature Secondary Structure 
TDKKSYYIFGTHASLAALNNPFRVIRYIYCTESCFLSLSKLIQKHNYKIVTTKFLHQLL
CCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
GSQIVHQGIAVNTIPLTINGIKNIDLNTPNYKIAILDQLLDHNNLGAIIRSAAAFDISTI
HHHHHHCCEEEEEEEEEEECEEEEECCCCCEEEEEEHHHHCCCCHHHHHHHHHHEEEEEE
ILPENNSIEENGTIAKIAVGALEKINLVKVKNLYNTINYLKKHEFWIIGLDLNDSNQLTE
EEECCCCCCCCCCEEEEEHHHHHHCEEEEHHHHHHHHHHHHCCCEEEEEEEECCCCHHHH
QTFHSKTAFVLGSENKGLRNLTRQQCDFLIKIPMSHKTESLNVAGAATIIFYESFKFSIK
HHHCCCEEEEEECCCCCHHHHHHHHCCEEEECCCCCCCCCEECCCEEEEEEEEEEEEEEE
QQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]