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The map label for this gene is glyA

Identifier: 148284135

GI number: 148284135

Start: 246995

End: 248275

Strand: Direct

Name: glyA

Synonym: OTBS_0253

Alternate gene names: 148284135

Gene position: 246995-248275 (Clockwise)

Preceding gene: 148284134

Following gene: 148284139

Centisome position: 11.61

GC content: 31.23

Gene sequence:

>1281_bases
ATGTGTAAAGAAATATATGATTTAATTGCTAAAGAATTATATAGACAGCAATCAACAATAGAATTAATAGCATCAGAGAA
TTTTACTAGTAAAGCAGTCATGCTAGCTCAAGGTTCTATTCTTACAAATAAATATGCAGAAGGATATATCAATAAAAGAT
ATTATGGTGGCTGTGAATTTATTGATGAGGTAGAAAGTATGGCTATTGATAGAGTAAAAAAGCTTTTTAAGTGCAACTAT
GCGAATGTACAACCACATTCTGGTTCTCAAGCTAATCAAGCAGTATTTTTAGCCTTGTTAAAGCCTGGTGATACAATTTT
AGCTATGGATTTAAATAGTGGTGGTCATTTAACCCATGGCGCTAAACCTAATATTTCTGGCAAATTTTTTAATGCAGTTC
ATTACTGTGTTAACAAAGATAGTTATTTAATTGACTATAATGAAGTTGAAATGCTAGCTCAGCAGCATAAACCAAAACTT
ATAATTGTTGGTTATTCAGCTTATTCAAGAAAGATAAATTTTGCAACATTTAAAGAAATAGCAGATAAGGTAGGAGCATA
TCTTTTAGCAGATATTGCGCATATTGCTGGATTAGTGGCTACAGAGTATCATTCTAGTCCTATACCATATGCTCATATTG
TAACTAGCACTACTCATAAGACTTTAAGAGGTCCAAGAGGTGGATTGATTCTAAGTAATGATGAATCAATAAGTAAAAAA
ATTAATTCTGCTGTATTTCCAGGTATGCAAGGCGGTCCATTAATGCATGTCATCGCTGCTAAAGCAATTGCTTTCTCTGA
AGCATTAATGCCTCAATATAAAGAATATATTAATCAAGTAATGTTAAATGCGAAGGTACTAGCTAAGTTACTACAAGACA
GAGGTTATAATATTTTAACAGGTGGAACAGATAATCATATGCTGCTAGTAGATTTAAGAGGAAAGAATATCACAGGACAA
GAAGCTGAATATTTACTAGATGCAGCTGGCATTACTTGTAATAAACAAGTAATGCCTTTTGATACTACTTCTCCAACCAT
TACATCTGGTATTAGATTAGGAACTCCAGCCTGTACTACTAGAGGCTTTAAAGAAAATGAATTTATTACTGTAGGAAAAT
ATATTGCTGATATTTTAGACAATATTGCAATAATTAAGTCTGCAAAAAAAAATGAAGGTATCACTATTACTGAAATTGAA
AAAAGTTTGAATAGTGTCATTACTAGAACAAAACATCATGTACAAGAATTATGTAATTGTTTTCCAATTTATACTAACTA
G

Upstream 100 bases:

>100_bases
TACTATTAAAGATACTGATTATAGTGATGATTGTAGCGATGATGAAAGTAATAACAATAACATCTAAAACTTGGTTATAA
GATAAAATTAGAGTTGGTAA

Downstream 100 bases:

>100_bases
ATAGCGCTGACAAACAACTAACTTAAACAACAGCAAGATCCATAAACACAAAGAAAGAAAAAGTAAGCTTAGTCTGGTAA
CAAAGAAGTGTTGCTATATC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MCKEIYDLIAKELYRQQSTIELIASENFTSKAVMLAQGSILTNKYAEGYINKRYYGGCEFIDEVESMAIDRVKKLFKCNY
ANVQPHSGSQANQAVFLALLKPGDTILAMDLNSGGHLTHGAKPNISGKFFNAVHYCVNKDSYLIDYNEVEMLAQQHKPKL
IIVGYSAYSRKINFATFKEIADKVGAYLLADIAHIAGLVATEYHSSPIPYAHIVTSTTHKTLRGPRGGLILSNDESISKK
INSAVFPGMQGGPLMHVIAAKAIAFSEALMPQYKEYINQVMLNAKVLAKLLQDRGYNILTGGTDNHMLLVDLRGKNITGQ
EAEYLLDAAGITCNKQVMPFDTTSPTITSGIRLGTPACTTRGFKENEFITVGKYIADILDNIAIIKSAKKNEGITITEIE
KSLNSVITRTKHHVQELCNCFPIYTN

Sequences:

>Translated_426_residues
MCKEIYDLIAKELYRQQSTIELIASENFTSKAVMLAQGSILTNKYAEGYINKRYYGGCEFIDEVESMAIDRVKKLFKCNY
ANVQPHSGSQANQAVFLALLKPGDTILAMDLNSGGHLTHGAKPNISGKFFNAVHYCVNKDSYLIDYNEVEMLAQQHKPKL
IIVGYSAYSRKINFATFKEIADKVGAYLLADIAHIAGLVATEYHSSPIPYAHIVTSTTHKTLRGPRGGLILSNDESISKK
INSAVFPGMQGGPLMHVIAAKAIAFSEALMPQYKEYINQVMLNAKVLAKLLQDRGYNILTGGTDNHMLLVDLRGKNITGQ
EAEYLLDAAGITCNKQVMPFDTTSPTITSGIRLGTPACTTRGFKENEFITVGKYIADILDNIAIIKSAKKNEGITITEIE
KSLNSVITRTKHHVQELCNCFPIYTN
>Mature_426_residues
MCKEIYDLIAKELYRQQSTIELIASENFTSKAVMLAQGSILTNKYAEGYINKRYYGGCEFIDEVESMAIDRVKKLFKCNY
ANVQPHSGSQANQAVFLALLKPGDTILAMDLNSGGHLTHGAKPNISGKFFNAVHYCVNKDSYLIDYNEVEMLAQQHKPKL
IIVGYSAYSRKINFATFKEIADKVGAYLLADIAHIAGLVATEYHSSPIPYAHIVTSTTHKTLRGPRGGLILSNDESISKK
INSAVFPGMQGGPLMHVIAAKAIAFSEALMPQYKEYINQVMLNAKVLAKLLQDRGYNILTGGTDNHMLLVDLRGKNITGQ
EAEYLLDAAGITCNKQVMPFDTTSPTITSGIRLGTPACTTRGFKENEFITVGKYIADILDNIAIIKSAKKNEGITITEIE
KSLNSVITRTKHHVQELCNCFPIYTN

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=446, Percent_Identity=42.8251121076233, Blast_Score=355, Evalue=4e-98,
Organism=Homo sapiens, GI261862350, Length=446, Percent_Identity=42.8251121076233, Blast_Score=355, Evalue=4e-98,
Organism=Homo sapiens, GI261862348, Length=446, Percent_Identity=42.8251121076233, Blast_Score=355, Evalue=4e-98,
Organism=Homo sapiens, GI19923315, Length=446, Percent_Identity=42.8251121076233, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI261862346, Length=446, Percent_Identity=41.7040358744395, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI22547186, Length=450, Percent_Identity=44.6666666666667, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI22547189, Length=436, Percent_Identity=41.9724770642202, Blast_Score=297, Evalue=1e-80,
Organism=Escherichia coli, GI1788902, Length=424, Percent_Identity=54.0094339622642, Blast_Score=447, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI25144732, Length=451, Percent_Identity=44.1241685144124, Blast_Score=354, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI25144729, Length=451, Percent_Identity=44.1241685144124, Blast_Score=354, Evalue=6e-98,
Organism=Saccharomyces cerevisiae, GI6319739, Length=395, Percent_Identity=44.8101265822785, Blast_Score=341, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6323087, Length=446, Percent_Identity=39.0134529147982, Blast_Score=312, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24640005, Length=448, Percent_Identity=43.5267857142857, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI221329721, Length=448, Percent_Identity=43.5267857142857, Blast_Score=356, Evalue=2e-98,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_ORITB (A5CCC4)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248225.1
- ProteinModelPortal:   A5CCC4
- STRING:   A5CCC4
- GeneID:   5218578
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_0253
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   NDEAIIK
- ProtClustDB:   PRK00011
- BioCyc:   OTSU357244:OTBS_0253-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 47008; Mature: 47008

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKEIYDLIAKELYRQQSTIELIASENFTSKAVMLAQGSILTNKYAEGYINKRYYGGCEF
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCHHH
IDEVESMAIDRVKKLFKCNYANVQPHSGSQANQAVFLALLKPGDTILAMDLNSGGHLTHG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCC
AKPNISGKFFNAVHYCVNKDSYLIDYNEVEMLAQQHKPKLIIVGYSAYSRKINFATFKEI
CCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCEEEEEEHHHCCCCCHHHHHHH
ADKVGAYLLADIAHIAGLVATEYHSSPIPYAHIVTSTTHKTLRGPRGGLILSNDESISKK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHCCCCCCEEEECCHHHHHH
INSAVFPGMQGGPLMHVIAAKAIAFSEALMPQYKEYINQVMLNAKVLAKLLQDRGYNILT
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
GGTDNHMLLVDLRGKNITGQEAEYLLDAAGITCNKQVMPFDTTSPTITSGIRLGTPACTT
CCCCCCEEEEEECCCCCCCCHHHHHHHHCCCEECCEECCCCCCCCCHHCCEECCCCHHHC
RGFKENEFITVGKYIADILDNIAIIKSAKKNEGITITEIEKSLNSVITRTKHHVQELCNC
CCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
FPIYTN
CCCCCC
>Mature Secondary Structure
MCKEIYDLIAKELYRQQSTIELIASENFTSKAVMLAQGSILTNKYAEGYINKRYYGGCEF
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCHHH
IDEVESMAIDRVKKLFKCNYANVQPHSGSQANQAVFLALLKPGDTILAMDLNSGGHLTHG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCC
AKPNISGKFFNAVHYCVNKDSYLIDYNEVEMLAQQHKPKLIIVGYSAYSRKINFATFKEI
CCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCEEEEEEHHHCCCCCHHHHHHH
ADKVGAYLLADIAHIAGLVATEYHSSPIPYAHIVTSTTHKTLRGPRGGLILSNDESISKK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHCCCCCCEEEECCHHHHHH
INSAVFPGMQGGPLMHVIAAKAIAFSEALMPQYKEYINQVMLNAKVLAKLLQDRGYNILT
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
GGTDNHMLLVDLRGKNITGQEAEYLLDAAGITCNKQVMPFDTTSPTITSGIRLGTPACTT
CCCCCCEEEEEECCCCCCCCHHHHHHHHCCCEECCEECCCCCCCCCHHCCEECCCCHHHC
RGFKENEFITVGKYIADILDNIAIIKSAKKNEGITITEIEKSLNSVITRTKHHVQELCNC
CCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
FPIYTN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA