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The map label for this gene is tpiA

Identifier: 148284061

GI number: 148284061

Start: 134361

End: 135110

Strand: Direct

Name: tpiA

Synonym: OTBS_0140

Alternate gene names: 148284061

Gene position: 134361-135110 (Clockwise)

Preceding gene: 148284060

Following gene: 148284062

Centisome position: 6.32

GC content: 28.8

Gene sequence:

>750_bases
ATGGAATCAATTGTAGTTTCTAATTGGAAAATGCACTTTAGTTTTTCTGAAGCTTGCAATTACCTAAATTTAATAACTAG
CTTACATGGCAATTTAAATATTGCTAAAGTGATATTTGCTGTACCAAATTTATATTTATCTGGGCTTAAATTAAAATTTA
ATGACACATATCATTTCTCAGCTCAAGATGTAAGCATGATTACAGAAAGTAGTGGCCCATATACTGGTGAGATAAGTGCT
AGTATGTTGAAAAACTTAAATGTGAACTATGCTATAGTAGGACACTCAGAACGAAGGTTATTATTTTATGAGGATGCTAA
TACAATAGCATCAAAAGTAAGAAATTGTATCAATAATGCAATAGTTCCAATTGTCTGTATAGGAGAACCAATTGAAGCAC
GAAAAAATAAAACATATCTTCAATATATAGCTCAACAGTTAAGTAGTATATCTTTTAGTTTTACTAAGAATATTATTATT
GCTTATGAGCCAGTATGGTCAATTGGCAGCTGTATGGTTCCTACCATTGATGATATTTATGAGGTAGTAACTATGATTAG
AGAAATACAAAATAGGTATATACCTCATAATATTGAAAATAGTGTTAAAATAGTATATGGTGGTTCTGTTTCAGCTAATA
ATATTAACCAAATTCTAACAGCTGGCATTGATGGAGTATTAATTGGTAAAGCATCTTTAAAATTAGAAAGTTTAACTACA
ATAATAAAAACAGTTCAAGGTTTAGATTAA

Upstream 100 bases:

>100_bases
TGTTCTTAATTTATTTAACACTGTAACTTGTATAATATATGTATATACCAATACAATATTTAAGTATTATAAATCTGCAA
GTAATTTTTTATCTTAAAGC

Downstream 100 bases:

>100_bases
ATAAAATGATGAATATACTACTTTTTATACATATTGTAATATCAGTATTATTAGTTACAGCAATATTACTTCACAAAACT
GGAGCGGATAGTTTAAGTGG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MESIVVSNWKMHFSFSEACNYLNLITSLHGNLNIAKVIFAVPNLYLSGLKLKFNDTYHFSAQDVSMITESSGPYTGEISA
SMLKNLNVNYAIVGHSERRLLFYEDANTIASKVRNCINNAIVPIVCIGEPIEARKNKTYLQYIAQQLSSISFSFTKNIII
AYEPVWSIGSCMVPTIDDIYEVVTMIREIQNRYIPHNIENSVKIVYGGSVSANNINQILTAGIDGVLIGKASLKLESLTT
IIKTVQGLD

Sequences:

>Translated_249_residues
MESIVVSNWKMHFSFSEACNYLNLITSLHGNLNIAKVIFAVPNLYLSGLKLKFNDTYHFSAQDVSMITESSGPYTGEISA
SMLKNLNVNYAIVGHSERRLLFYEDANTIASKVRNCINNAIVPIVCIGEPIEARKNKTYLQYIAQQLSSISFSFTKNIII
AYEPVWSIGSCMVPTIDDIYEVVTMIREIQNRYIPHNIENSVKIVYGGSVSANNINQILTAGIDGVLIGKASLKLESLTT
IIKTVQGLD
>Mature_249_residues
MESIVVSNWKMHFSFSEACNYLNLITSLHGNLNIAKVIFAVPNLYLSGLKLKFNDTYHFSAQDVSMITESSGPYTGEISA
SMLKNLNVNYAIVGHSERRLLFYEDANTIASKVRNCINNAIVPIVCIGEPIEARKNKTYLQYIAQQLSSISFSFTKNIII
AYEPVWSIGSCMVPTIDDIYEVVTMIREIQNRYIPHNIENSVKIVYGGSVSANNINQILTAGIDGVLIGKASLKLESLTT
IIKTVQGLD

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=242, Percent_Identity=30.5785123966942, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4507645, Length=241, Percent_Identity=30.7053941908714, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI1790353, Length=250, Percent_Identity=32.8, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17536593, Length=235, Percent_Identity=33.6170212765957, Blast_Score=127, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6320255, Length=171, Percent_Identity=34.5029239766082, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28572008, Length=235, Percent_Identity=32.7659574468085, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI28572006, Length=235, Percent_Identity=32.7659574468085, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI28572004, Length=235, Percent_Identity=32.7659574468085, Blast_Score=129, Evalue=1e-30,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_ORITB (A5CC41)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248151.1
- ProteinModelPortal:   A5CC41
- SMR:   A5CC41
- STRING:   A5CC41
- GeneID:   5218459
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_0140
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   NCHESKD
- ProtClustDB:   CLSK2325607
- BioCyc:   OTSU357244:OTBS_0140-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27653; Mature: 27653

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 163-163 BINDING 8-8 BINDING 10-10

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIVVSNWKMHFSFSEACNYLNLITSLHGNLNIAKVIFAVPNLYLSGLKLKFNDTYHFS
CCCEEEECEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCEEEEC
AQDVSMITESSGPYTGEISASMLKNLNVNYAIVGHSERRLLFYEDANTIASKVRNCINNA
CCHHHHEECCCCCCCCCHHHHHHHCCCCCEEEEECCCCEEEEEECHHHHHHHHHHHHCCC
IVPIVCIGEPIEARKNKTYLQYIAQQLSSISFSFTKNIIIAYEPVWSIGSCMVPTIDDIY
CEEEEEECCCHHHHCCHHHHHHHHHHHHHCEEEEECCEEEEECCCCCCCCCCCCCHHHHH
EVVTMIREIQNRYIPHNIENSVKIVYGGSVSANNINQILTAGIDGVLIGKASLKLESLTT
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCEEEEECCHHHHHHHHH
IIKTVQGLD
HHHHHHCCC
>Mature Secondary Structure
MESIVVSNWKMHFSFSEACNYLNLITSLHGNLNIAKVIFAVPNLYLSGLKLKFNDTYHFS
CCCEEEECEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCEEEEC
AQDVSMITESSGPYTGEISASMLKNLNVNYAIVGHSERRLLFYEDANTIASKVRNCINNA
CCHHHHEECCCCCCCCCHHHHHHHCCCCCEEEEECCCCEEEEEECHHHHHHHHHHHHCCC
IVPIVCIGEPIEARKNKTYLQYIAQQLSSISFSFTKNIIIAYEPVWSIGSCMVPTIDDIY
CEEEEEECCCHHHHCCHHHHHHHHHHHHHCEEEEECCEEEEECCCCCCCCCCCCCHHHHH
EVVTMIREIQNRYIPHNIENSVKIVYGGSVSANNINQILTAGIDGVLIGKASLKLESLTT
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCEEEEECCHHHHHHHHH
IIKTVQGLD
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA