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The map label for this gene is lacA [C]

Identifier: 148268987

GI number: 148268987

Start: 2729874

End: 2730473

Strand: Reverse

Name: lacA [C]

Synonym: SaurJH9_2577

Alternate gene names: 148268987

Gene position: 2730473-2729874 (Counterclockwise)

Preceding gene: 148268991

Following gene: 148268986

Centisome position: 93.94

GC content: 36.0

Gene sequence:

>600_bases
ATGACTGAAAAAGAAAAAATGTTAGCAGAAAAATGGTACGATGCAAACTTTGATCAAGACTTAATCAATGAACGTGCACG
AGCGAAAGATATTTGCTTTGAATTAAATCATACAAAGCCGAGTGATACAAATAAAAGAAAGGAATTAATCGATCAATTAT
TTCAAACAACAACAGACAATGTAAGTATTTCGATTCCTTTTGATACAGATTATGGTTGGAACGTTAAACTAGGAAAAAAT
GTCTATGTAAACACCAATTGTTATTTTATGGATGGTGGACAGATTACAATTGGCGATAATGTTTTTATAGGACCTAATTG
TGGATTCTACACAGCAACACATCCACTTAATTTTTATCATAGAAATGAAGGTTATGAAAAAGCCGGACCCATTCATATTG
GCAGTAATACTTGGTTTGGCGGTCATGTAGCCGTGTTACCAGGGGTGACGATTGGAGAAGGCAGTGTGATTGGTGCTGGT
AGTGTTGTCACTAAAGATATTCCGCCACACAGCTTAGCGGTTGGAAACCCTTGTAAAGTCGTTCGTAAAATTGATAATGA
CCTACCATCCGAAACATTAAATGACGAAACGATAAAGTAG

Upstream 100 bases:

>100_bases
TCATGATTTCATAATGTTATAGCGTATTAGTTGCATAGGCGAATGTTTATTTGATTAACTTTTAAACAAGTAAATAAGTT
ATTAGGAGTTGAAAGGTGTA

Downstream 100 bases:

>100_bases
CAGTTGCGACCCATATAAATTTTAAAAAGTAACCAATCAATTTCAAAATACTTGAGAATAAGTGATAAAGCATAAAAATG
AAAGCGATTGCAAAACTAAC

Product: hexapaptide repeat-containing transferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MTEKEKMLAEKWYDANFDQDLINERARAKDICFELNHTKPSDTNKRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKN
VYVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFYHRNEGYEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK

Sequences:

>Translated_199_residues
MTEKEKMLAEKWYDANFDQDLINERARAKDICFELNHTKPSDTNKRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKN
VYVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFYHRNEGYEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK
>Mature_198_residues
TEKEKMLAEKWYDANFDQDLINERARAKDICFELNHTKPSDTNKRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNV
YVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFYHRNEGYEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAGS
VVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1786537, Length=186, Percent_Identity=42.4731182795699, Blast_Score=160, Evalue=6e-41,
Organism=Escherichia coli, GI1786664, Length=183, Percent_Identity=39.344262295082, Blast_Score=147, Evalue=4e-37,
Organism=Escherichia coli, GI1788367, Length=146, Percent_Identity=32.1917808219178, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322243, Length=184, Percent_Identity=48.3695652173913, Blast_Score=185, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ATRF2_STAA1 (Q9KWJ8)

Other databases:

- EMBL:   AB035451
- EMBL:   AP009324
- RefSeq:   YP_001443129.1
- ProteinModelPortal:   Q9KWJ8
- SMR:   Q9KWJ8
- STRING:   Q9KWJ8
- EnsemblBacteria:   EBSTAT00000005189
- GeneID:   5560127
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2539
- eggNOG:   COG0110
- GeneTree:   EBGT00050000024444
- HOGENOM:   HBG699746
- OMA:   EPNFFCD
- BioCyc:   SAUR418127:SAHV_2539-MONOMER
- InterPro:   IPR001451
- InterPro:   IPR011004

Pfam domain/function: PF00132 Hexapep; SSF51161 Trimer_LpxA_like

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 22129; Mature: 21998

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKEKMLAEKWYDANFDQDLINERARAKDICFELNHTKPSDTNKRKELIDQLFQTTTDN
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
VSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFYH
EEEEEECCCCCCCEEEECCEEEECCEEEEEECCEEEECCEEEECCCCCEEEECCCEEEEE
RNEGYEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAGSVVTKDIPPHSLAVGNPCKV
CCCCCCCCCCEEECCCCCCCCEEEEECCEEECCCCEEECCCEEECCCCCCCEECCCHHHH
VRKIDNDLPSETLNDETIK
HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEKEKMLAEKWYDANFDQDLINERARAKDICFELNHTKPSDTNKRKELIDQLFQTTTDN
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
VSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFYH
EEEEEECCCCCCCEEEECCEEEECCEEEEEECCEEEECCEEEECCCCCEEEECCCEEEEE
RNEGYEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAGSVVTKDIPPHSLAVGNPCKV
CCCCCCCCCCEEECCCCCCCCEEEEECCEEECCCCEEECCCEEECCCCCCCEECCCHHHH
VRKIDNDLPSETLNDETIK
HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580