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The map label for this gene is brnQ [C]

Identifier: 148267895

GI number: 148267895

Start: 1536413

End: 1537756

Strand: Reverse

Name: brnQ [C]

Synonym: SaurJH9_1468

Alternate gene names: 148267895

Gene position: 1537756-1536413 (Counterclockwise)

Preceding gene: 148267896

Following gene: 148267891

Centisome position: 52.9

GC content: 32.22

Gene sequence:

>1344_bases
ATGAATAAAAATACATGGGTCATTGGGTTTACGCTCTTCGCTATGTTTTTTGGCGCAGGCAATCTAATTTTTCCTCCTAA
TCTCGGATTGGATAGTGGTCAATTTTTCTGGCCCGCCATTTTAGCGTTTGTTCTAACTGGGATTGGTTTACCATTATTAG
GTGTGATTGTAGGTGCACTTGATAAAGAAGGATATATTGGCGCATTAAATAAAATTTCACCTAAATTTTCAATATTGTTC
TTAATCATCATTTATTTGACTATAGGACCACTTTTCGCAATACCTAGAACTGCATCTACATCTTTTGAAATGACAATTAC
ACCAATTATACATAGCAATAGTAGTATCGCTTTATTTATATTTACGATTATCTACTTCATAGTCGTTTTGTATATTTGTT
TAAATCCATCTAAGTTAATCGATCGTATTGGTTCATTATTAACACCATTATTATTGATTACTATTTTAGCGATGATTATT
AAAGGATACTTAGACTTTAGCGGTAATAGTGCTGGAAAGGGCAATGAAGCACTATATCATTCTAATTTTTCAAGTTTTGC
TGAAGGCTTTACACAAGGCTATTTAACAATGGATGCCATTGCAGCAATTGCTTTTTCAATGATTGTTGTTAATGCAGTAA
AACTAACAGGCATTACTAAAACAAATCAAATATTCAAACAAACTTTGACTGCTGGTTTAATTGCAGCCGTAGCTTTAATT
TTCATATATATTTCATTAGGTTATATTGGTAATCATATGCCAGTAAGTGACATGACGTTAGATCAATTGAAATCCAAAGA
TCGAAACATTGGGACATATTTATTAACGACAATGGCTTCAATAGGATTTGGTTCATTCGGAAAATATTTATTGGGCATCA
TTGTGGCGCTGGCATGTCTAACTACAGCATGCGGGCTTATTGTTGCAGTTTCTGAATATTTCCATAGAATCGTACCTAAA
GTATCATACAAAGCATTTGTATTAGTTTTCATTTTAATGAGTTTTATTATTGCTAACCAAGGTTTAAATGCTGTTATCTC
AATGTCAATTCCGGTATTAAGCATTGTATACCCAGTAGCAATAACTGTTGTATTATTAATTTTAATTGCCAAATTCATAC
CGACAAAACGCATTTCACAACAAATTCCAGTTATTATCGTATTTATATTGTCGATTTTCAGTGTTATTAGTAAGTTAGGT
TGGCTGAAAATTAACTTTATAGAATCATTGCCTCTAAGAGCGTATTCTTTAGAGTGGTTCCCAGTAGCAATTATTGCAAC
GATATTAGGCTATCTAGTCGGCATATTTGTAAAACAAGATCCAATTAAATATCAACAGGAATAA

Upstream 100 bases:

>100_bases
TCTGCTATGTACAACATATTTCATATAGACTCTTTATTATTTTTTGTGCTAAAATATTTACTTGTAAATTATTTCGTGTT
TTAAAAAAAGGAGTAACTCT

Downstream 100 bases:

>100_bases
CGAATAATATAAAAGGAAGCATCTCCAATTTAATCGTAGGATGCTTCCTTTTTGTTATATCTAATTCGTAATTTATGATT
GTTTATTCGGTCCTTTGATG

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILF
LIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASIGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPK
VSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLG
WLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE

Sequences:

>Translated_447_residues
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILF
LIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASIGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPK
VSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLG
WLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE
>Mature_447_residues
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILF
LIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASIGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPK
VSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLG
WLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine), which is coupled to a proton motive force (Potential). Contributes to NaCl tolerance [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=434, Percent_Identity=33.4101382488479, Blast_Score=232, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 48827; Mature: 48827

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIGLPLLGVIVGAL
CCCCCEEHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
DKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFI
CCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCHHHHH
FTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMIIKGYLDFSGNSAGKGNEALYH
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
SNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASIGFGSFGKYLLGIIVALACL
HHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLGWLKINFIESLPLRAYSLEWF
HHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEHHHCCCCCEEECCHH
PVAIIATILGYLVGIFVKQDPIKYQQE
HHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIGLPLLGVIVGAL
CCCCCEEHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
DKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFI
CCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCHHHHH
FTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMIIKGYLDFSGNSAGKGNEALYH
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
SNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASIGFGSFGKYLLGIIVALACL
HHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLGWLKINFIESLPLRAYSLEWF
HHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEHHHCCCCCEEECCHH
PVAIIATILGYLVGIFVKQDPIKYQQE
HHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA