Click here to switch to the map view.

The map label for this gene is fixL [H]

Identifier: 148261127

GI number: 148261127

Start: 2358979

End: 2360376

Strand: Reverse

Name: fixL [H]

Synonym: Acry_2136

Alternate gene names: 148261127

Gene position: 2360376-2358979 (Counterclockwise)

Preceding gene: 148261128

Following gene: 148261125

Centisome position: 69.64

GC content: 66.52

Gene sequence:

>1398_bases
ATGAACAAAGTCTTCGGGCGATGGATCGGCCACAGCCTGTCGGCGCGGCTGCTCTGGCTGACCGTGATCGTGACGCTGCT
TGTCGAGGTCATGATCCTGTTGCCGAGCCTCGGCCATGAACGCAGCGCCTGGCTGATGAATAGAGTGACCGAGGCCCATC
TCGCGGCGCTGACGGTATCGACCGCGCCGCATGGGGTCGTCGATCTGTCGACTCGCAACGAATTGCTCCGTCTGTCCGGC
ACCGAGGCGATCCGGCTCGTGCAGGGCGGACGCAGCATCCTGGTGTTGCCGCCGCGGGGGACGGTGCGCGCCGAGGGCTC
GATCAACCTCGCGCACGAAACCCTGTTTGGTTCCGTTCTCAGGGCTCTCGATGTGCAGTTCGGCCTTGCCCCGCGCGATG
TGCTGGTCACCGCGCCAAGTCCGCTGCAGCCGAATGTCATCGTGCAGATCGTCGTCCACAATCCCGTTCTCGCAAGGCAT
CTGTGGCACTATGCGGACCATACCGCGGCCCTCTCTCTCGCCATCGCGCTGCTGACCGGACTGGTGGTCTATGTCGCGCT
CAACCGGGTGCTCGTGCTGCCGATGCGCCGGCTGACGGAAAGCATCACCCGCTTCCGGGAAGATCCCGAGCACGCCGCGC
CGCTTGCGATCCGGCCGGCGGATGAGTTCAGGGACGACGAGATCGCGGTTGCCGCGCGCGAACTCGCAAGCATGCAGACG
GAGCTTCGTGCCGCGCTGTGGCGCAACGCCAGGCTGGCGGCACTCGGCACGGCGGTGGCCAAGATCAGCCACGATCTGCG
CAACATATTATCGACGGCGTTGCTGGTCGCTGACCGGCTTTCCGGAAGTGCTGACCCCGCGATCCGCAGCGGCGCGACGA
CCCTGGTCGACGCGGTCGAACGCGCGACGGATCTCGTCACTCATACGCTCAGTTTCGCCCGGGAGGGCCCGCCGCCTCTG
GTCAGAGCGGCCTGCCCCCTGGCGGAACTGGTCGCGGACGTCGCCGCGCAGACGCGCCTGCTGCACGAGCGCCTGACCAT
CGAAACGAACATTCCAGCTGAAATCATGCCGTCGCTGGACAGAACGCAGATGCAGCGCGCCTTGCTGAACCTGATGCGCA
ACGCCGCCGAGGCGGGGGCGGGCCGGCTCGTCATCAGCAGCGGCATCGATGCCGGCCAGGTCATCATCGACATCGTGGAT
GACGGACCTGGTCTGCCCGAGCGGGCGCAGGCCAATCTGTTCAGGCCCTTCACAGGGTCTGCCCGCAGCGGTGGAACGGG
CCTGGGCCTCGCCATCGCCCGTGACCTGGTGCGCGCCCATGGAGGCGAGCTTACGCTCGTGGCGACGAGCAGCAGAGGAA
CGCATTTCAGGATATCGATCCCGCTCGACCCCGGCTGA

Upstream 100 bases:

>100_bases
GCCTTTCGCCCGAATCGCGGGCCTATGCGGACCGTGCCTTTCTCGATCGCCTGTTCGAGGAAGGGCGCGCCGCTTTCCGC
GCGAGGGCCGCCTGACCGCC

Downstream 100 bases:

>100_bases
CCGGCCGTCACGCAGGGTGAGGAGACGTTCCAGTCGCAACTAGAAATGTATGGGCGGAAAGCTTCTCACCAGGCTGCCGC
AGACGAGTTGCCAGCCATCG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MNKVFGRWIGHSLSARLLWLTVIVTLLVEVMILLPSLGHERSAWLMNRVTEAHLAALTVSTAPHGVVDLSTRNELLRLSG
TEAIRLVQGGRSILVLPPRGTVRAEGSINLAHETLFGSVLRALDVQFGLAPRDVLVTAPSPLQPNVIVQIVVHNPVLARH
LWHYADHTAALSLAIALLTGLVVYVALNRVLVLPMRRLTESITRFREDPEHAAPLAIRPADEFRDDEIAVAARELASMQT
ELRAALWRNARLAALGTAVAKISHDLRNILSTALLVADRLSGSADPAIRSGATTLVDAVERATDLVTHTLSFAREGPPPL
VRAACPLAELVADVAAQTRLLHERLTIETNIPAEIMPSLDRTQMQRALLNLMRNAAEAGAGRLVISSGIDAGQVIIDIVD
DGPGLPERAQANLFRPFTGSARSGGTGLGLAIARDLVRAHGGELTLVATSSRGTHFRISIPLDPG

Sequences:

>Translated_465_residues
MNKVFGRWIGHSLSARLLWLTVIVTLLVEVMILLPSLGHERSAWLMNRVTEAHLAALTVSTAPHGVVDLSTRNELLRLSG
TEAIRLVQGGRSILVLPPRGTVRAEGSINLAHETLFGSVLRALDVQFGLAPRDVLVTAPSPLQPNVIVQIVVHNPVLARH
LWHYADHTAALSLAIALLTGLVVYVALNRVLVLPMRRLTESITRFREDPEHAAPLAIRPADEFRDDEIAVAARELASMQT
ELRAALWRNARLAALGTAVAKISHDLRNILSTALLVADRLSGSADPAIRSGATTLVDAVERATDLVTHTLSFAREGPPPL
VRAACPLAELVADVAAQTRLLHERLTIETNIPAEIMPSLDRTQMQRALLNLMRNAAEAGAGRLVISSGIDAGQVIIDIVD
DGPGLPERAQANLFRPFTGSARSGGTGLGLAIARDLVRAHGGELTLVATSSRGTHFRISIPLDPG
>Mature_465_residues
MNKVFGRWIGHSLSARLLWLTVIVTLLVEVMILLPSLGHERSAWLMNRVTEAHLAALTVSTAPHGVVDLSTRNELLRLSG
TEAIRLVQGGRSILVLPPRGTVRAEGSINLAHETLFGSVLRALDVQFGLAPRDVLVTAPSPLQPNVIVQIVVHNPVLARH
LWHYADHTAALSLAIALLTGLVVYVALNRVLVLPMRRLTESITRFREDPEHAAPLAIRPADEFRDDEIAVAARELASMQT
ELRAALWRNARLAALGTAVAKISHDLRNILSTALLVADRLSGSADPAIRSGATTLVDAVERATDLVTHTLSFAREGPPPL
VRAACPLAELVADVAAQTRLLHERLTIETNIPAEIMPSLDRTQMQRALLNLMRNAAEAGAGRLVISSGIDAGQVIIDIVD
DGPGLPERAQANLFRPFTGSARSGGTGLGLAIARDLVRAHGGELTLVATSSRGTHFRISIPLDPG

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI87082128, Length=313, Percent_Identity=26.1980830670927, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789808, Length=202, Percent_Identity=31.6831683168317, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788549, Length=236, Percent_Identity=27.1186440677966, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786912, Length=245, Percent_Identity=31.8367346938775, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=28.9915966386555, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790300, Length=259, Percent_Identity=27.4131274131274, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=225, Percent_Identity=27.5555555555556, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081816, Length=118, Percent_Identity=33.0508474576271, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1790346, Length=219, Percent_Identity=27.3972602739726, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788393, Length=250, Percent_Identity=27.2, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49939; Mature: 49939

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVFGRWIGHSLSARLLWLTVIVTLLVEVMILLPSLGHERSAWLMNRVTEAHLAALTVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEC
TAPHGVVDLSTRNELLRLSGTEAIRLVQGGRSILVLPPRGTVRAEGSINLAHETLFGSVL
CCCCCEEEECCCHHHHEECCCHHEEEEECCCEEEEECCCCCEEECCCCCHHHHHHHHHHH
RALDVQFGLAPRDVLVTAPSPLQPNVIVQIVVHNPVLARHLWHYADHTAALSLAIALLTG
HHHHHHCCCCCCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVVYVALNRVLVLPMRRLTESITRFREDPEHAAPLAIRPADEFRDDEIAVAARELASMQT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHH
ELRAALWRNARLAALGTAVAKISHDLRNILSTALLVADRLSGSADPAIRSGATTLVDAVE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHH
RATDLVTHTLSFAREGPPPLVRAACPLAELVADVAAQTRLLHERLTIETNIPAEIMPSLD
HHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEEECCCCHHHHCCCH
RTQMQRALLNLMRNAAEAGAGRLVISSGIDAGQVIIDIVDDGPGLPERAQANLFRPFTGS
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHCCCCCCCCCC
ARSGGTGLGLAIARDLVRAHGGELTLVATSSRGTHFRISIPLDPG
CCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCC
>Mature Secondary Structure
MNKVFGRWIGHSLSARLLWLTVIVTLLVEVMILLPSLGHERSAWLMNRVTEAHLAALTVS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEC
TAPHGVVDLSTRNELLRLSGTEAIRLVQGGRSILVLPPRGTVRAEGSINLAHETLFGSVL
CCCCCEEEECCCHHHHEECCCHHEEEEECCCEEEEECCCCCEEECCCCCHHHHHHHHHHH
RALDVQFGLAPRDVLVTAPSPLQPNVIVQIVVHNPVLARHLWHYADHTAALSLAIALLTG
HHHHHHCCCCCCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVVYVALNRVLVLPMRRLTESITRFREDPEHAAPLAIRPADEFRDDEIAVAARELASMQT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHH
ELRAALWRNARLAALGTAVAKISHDLRNILSTALLVADRLSGSADPAIRSGATTLVDAVE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHH
RATDLVTHTLSFAREGPPPLVRAACPLAELVADVAAQTRLLHERLTIETNIPAEIMPSLD
HHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEEECCCCHHHHCCCH
RTQMQRALLNLMRNAAEAGAGRLVISSGIDAGQVIIDIVDDGPGLPERAQANLFRPFTGS
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHCCCCCCCCCC
ARSGGTGLGLAIARDLVRAHGGELTLVATSSRGTHFRISIPLDPG
CCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 8432704 [H]