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The map label for this gene is rpoN [H]

Identifier: 148261007

GI number: 148261007

Start: 2211195

End: 2212667

Strand: Reverse

Name: rpoN [H]

Synonym: Acry_2015

Alternate gene names: 148261007

Gene position: 2212667-2211195 (Counterclockwise)

Preceding gene: 148261008

Following gene: 148261006

Centisome position: 65.29

GC content: 62.8

Gene sequence:

>1473_bases
ATGGCAATAGGACCTCGTCTCGATCTGCGACATACTCAGTCGCTGGTGATGACGCCTCAGCTGCGGCAGGCGATCCAGTT
GCTGCAGTTCACGAATGCCGAGGTCAGCCAGTTCATCGAGGATGAACTTCTCAAGAACCCGCTGCTCGAACGGGTGGAGC
CCGAAGCCGACGCGGCGCTGCCCGTGCCGAGGAACGACGCTGCTTCCACCGAGAAGCCGACGGGCGATATTGGCGCGGAA
CATGTGGTCGATCCGGATCCCTCCGGCGCGAGCACGCCGCTCGATGTCGATTTTTCGAATGTCTATGACGCTGGCGGCGT
GGGTGATGGAACCGGTTCCGGCCTTTCGCCGGAGCGGGATGACTGGATCGGCCAGATTGCCGACCGGCCGCCGGGGTTGC
GGGAAAGCCTCGATCAGCAGGCGAGGCTCGCCTTTGCCGACCCGGTGCGCCGCAGGATTGCGTTCGCACTCATCATGGCG
CTCGACCCTGCCGGACGCCTGGCCGACCCGCCCGAGCAGATCGCCGAACGTCTTGCAGTCGCCCCCGACATGCTGGAGGC
GGTGCGTCAGACGATGATGCGTTTCGACCCGACCGGCATGTTTGCCCGCGATCTGGCCGAATGTCTCGCGGTTCAGCTCG
ACGAGCGCAATAGGCTGGACCCTGCCATGCGCGCGCTGCTTGCCAATCTCGAACTTCTTGCCAGGCGCGATCTTCGGTCA
CTGATGGAGATCTGCGGCGTCGATGCCGAAGATCTGCGCGACATGATTGCCGAAATCCGCCGCCTCAACCCCAAGCCGGG
GGCGATCTTCGATGTCACCCCCATGACGCCGTTGATCCCTGATGTCCTGATGCGCCCGGGGCCGGATGGCGACTATTTTC
TCGAACTGAACCCCGAGACCATGCCCCGCGTGCTCATCCGCCGAGGGTTCCACGCCCAAATCGTCGCTCGTGCGTCCCGC
GAGACGCGGCAATTTCTTTCCGAACGCGTACAAAGCGCGAACTGGCTGGTACGTGCCCTCGAGTCCCGCGCGGAGACGAT
CCTGCGCGTCGCCGGCGAGATTGTGCGACACCAGGACGCCTTCTTTCGCCATGGGATCGGGTTTCTCCGGCCATTGACCC
TGCGCGAAATTGCCGCCGCTCTCGAGATACATGAAAGTACGGTTAGCCGTGTCACGTCGAACAAGGTGATCGCCACACCG
CGCGGCATTTTCGAGATGAAATTTTTCTTCACGACCGCGCTTGCGGGCGCGAATGGCGAAAGCCACAGCGCCGAGGCGGT
GCGCCATCGCATCTCGACGCTGATCGAGGGTGAGGCGGCGAACCGAATCCTCTCCGACGATGCGCTTGCCAGATTGTTAC
AAAAGGAAGGCATAGACATAGCCCGCCGAACTGTGGCCAAATACAGGGAAGCGTTGCGCATTCCCGGCTCGGCGCAACGC
AAGCGCGAAAAAGGGCTTCTGGCAGGAGGCTGA

Upstream 100 bases:

>100_bases
TGTTGCGCGCCGCTACAGTGGCAACAATTCTTGATGCAAAAAGCGCACCATGCGCGTTCTGCCCGTTCCGCATTGCTGAT
GGCTTTGGTATGATCCATGC

Downstream 100 bases:

>100_bases
AGCGCGTCGCGCGAGGGCCGGCGTCATAAAGACTTGTCGATTATTGAGACGTATCCGTGCCGACGTAAACGCCCGCTTCA
CCTGGCGGCCCTACAATGCT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MAIGPRLDLRHTQSLVMTPQLRQAIQLLQFTNAEVSQFIEDELLKNPLLERVEPEADAALPVPRNDAASTEKPTGDIGAE
HVVDPDPSGASTPLDVDFSNVYDAGGVGDGTGSGLSPERDDWIGQIADRPPGLRESLDQQARLAFADPVRRRIAFALIMA
LDPAGRLADPPEQIAERLAVAPDMLEAVRQTMMRFDPTGMFARDLAECLAVQLDERNRLDPAMRALLANLELLARRDLRS
LMEICGVDAEDLRDMIAEIRRLNPKPGAIFDVTPMTPLIPDVLMRPGPDGDYFLELNPETMPRVLIRRGFHAQIVARASR
ETRQFLSERVQSANWLVRALESRAETILRVAGEIVRHQDAFFRHGIGFLRPLTLREIAAALEIHESTVSRVTSNKVIATP
RGIFEMKFFFTTALAGANGESHSAEAVRHRISTLIEGEAANRILSDDALARLLQKEGIDIARRTVAKYREALRIPGSAQR
KREKGLLAGG

Sequences:

>Translated_490_residues
MAIGPRLDLRHTQSLVMTPQLRQAIQLLQFTNAEVSQFIEDELLKNPLLERVEPEADAALPVPRNDAASTEKPTGDIGAE
HVVDPDPSGASTPLDVDFSNVYDAGGVGDGTGSGLSPERDDWIGQIADRPPGLRESLDQQARLAFADPVRRRIAFALIMA
LDPAGRLADPPEQIAERLAVAPDMLEAVRQTMMRFDPTGMFARDLAECLAVQLDERNRLDPAMRALLANLELLARRDLRS
LMEICGVDAEDLRDMIAEIRRLNPKPGAIFDVTPMTPLIPDVLMRPGPDGDYFLELNPETMPRVLIRRGFHAQIVARASR
ETRQFLSERVQSANWLVRALESRAETILRVAGEIVRHQDAFFRHGIGFLRPLTLREIAAALEIHESTVSRVTSNKVIATP
RGIFEMKFFFTTALAGANGESHSAEAVRHRISTLIEGEAANRILSDDALARLLQKEGIDIARRTVAKYREALRIPGSAQR
KREKGLLAGG
>Mature_489_residues
AIGPRLDLRHTQSLVMTPQLRQAIQLLQFTNAEVSQFIEDELLKNPLLERVEPEADAALPVPRNDAASTEKPTGDIGAEH
VVDPDPSGASTPLDVDFSNVYDAGGVGDGTGSGLSPERDDWIGQIADRPPGLRESLDQQARLAFADPVRRRIAFALIMAL
DPAGRLADPPEQIAERLAVAPDMLEAVRQTMMRFDPTGMFARDLAECLAVQLDERNRLDPAMRALLANLELLARRDLRSL
MEICGVDAEDLRDMIAEIRRLNPKPGAIFDVTPMTPLIPDVLMRPGPDGDYFLELNPETMPRVLIRRGFHAQIVARASRE
TRQFLSERVQSANWLVRALESRAETILRVAGEIVRHQDAFFRHGIGFLRPLTLREIAAALEIHESTVSRVTSNKVIATPR
GIFEMKFFFTTALAGANGESHSAEAVRHRISTLIEGEAANRILSDDALARLLQKEGIDIARRTVAKYREALRIPGSAQRK
REKGLLAGG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes (nif operon), glnA and dctA for dicar

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=483, Percent_Identity=36.4389233954451, Blast_Score=288, Evalue=6e-79,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54159; Mature: 54028

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIGPRLDLRHTQSLVMTPQLRQAIQLLQFTNAEVSQFIEDELLKNPLLERVEPEADAAL
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PVPRNDAASTEKPTGDIGAEHVVDPDPSGASTPLDVDFSNVYDAGGVGDGTGSGLSPERD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHH
DWIGQIADRPPGLRESLDQQARLAFADPVRRRIAFALIMALDPAGRLADPPEQIAERLAV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
APDMLEAVRQTMMRFDPTGMFARDLAECLAVQLDERNRLDPAMRALLANLELLARRDLRS
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LMEICGVDAEDLRDMIAEIRRLNPKPGAIFDVTPMTPLIPDVLMRPGPDGDYFLELNPET
HHHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHCCCCCCCEEEEECCHH
MPRVLIRRGFHAQIVARASRETRQFLSERVQSANWLVRALESRAETILRVAGEIVRHQDA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFRHGIGFLRPLTLREIAAALEIHESTVSRVTSNKVIATPRGIFEMKFFFTTALAGANGE
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCC
SHSAEAVRHRISTLIEGEAANRILSDDALARLLQKEGIDIARRTVAKYREALRIPGSAQR
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHH
KREKGLLAGG
HHHCCCCCCC
>Mature Secondary Structure 
AIGPRLDLRHTQSLVMTPQLRQAIQLLQFTNAEVSQFIEDELLKNPLLERVEPEADAAL
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PVPRNDAASTEKPTGDIGAEHVVDPDPSGASTPLDVDFSNVYDAGGVGDGTGSGLSPERD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHH
DWIGQIADRPPGLRESLDQQARLAFADPVRRRIAFALIMALDPAGRLADPPEQIAERLAV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
APDMLEAVRQTMMRFDPTGMFARDLAECLAVQLDERNRLDPAMRALLANLELLARRDLRS
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LMEICGVDAEDLRDMIAEIRRLNPKPGAIFDVTPMTPLIPDVLMRPGPDGDYFLELNPET
HHHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHCCCCCCCEEEEECCHH
MPRVLIRRGFHAQIVARASRETRQFLSERVQSANWLVRALESRAETILRVAGEIVRHQDA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFRHGIGFLRPLTLREIAAALEIHESTVSRVTSNKVIATPRGIFEMKFFFTTALAGANGE
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCC
SHSAEAVRHRISTLIEGEAANRILSDDALARLLQKEGIDIARRTVAKYREALRIPGSAQR
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHH
KREKGLLAGG
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2211497 [H]