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The map label for this gene is tuf

Identifier: 148260941

GI number: 148260941

Start: 2133085

End: 2134272

Strand: Reverse

Name: tuf

Synonym: Acry_1948

Alternate gene names: 148260941

Gene position: 2134272-2133085 (Counterclockwise)

Preceding gene: 148260942

Following gene: 148260940

Centisome position: 62.97

GC content: 65.49

Gene sequence:

>1188_bases
ATGGCCAAGGCTAAATTCGAGCGGACGAAGCCGCACTGCAACATCGGCACGATCGGCCACGTCGATCATGGCAAGACCTC
GCTCACCGCGGCGATCACCAAGGTGCTGGCCGAATCCGGTGGTGCGACCTTCCGCGCCTATGACAGCATCGACGCCGCTC
CCGAGGAGCGCGCCCGCGGCATCACGATCGCGACCGCGCATGTCGAGTACGAGACCGCGAACCGCCACTACGCGCATGTC
GACTGCCCTGGCCACGCCGACTACGTGAAGAACATGATCACCGGCGCGGCGCAGATGGATGGCGCGATCCTGGTCGTCTC
CGCCGCCGATGGCCCGATGCCGCAGACCCGCGAGCACATCCTGCTCGCCCGCCAGGTCGGCGTGCCGGCGCTCGTCGTGT
TCCTCAACAAGATGGACATGGCCGACCCCGACCTCGTCGAGCTGGTCGAGATGGAAGTCCGCGACCTGCTGAGCAAGTAT
GAGTTCCCGGGCGACGACATCCCCATCATCAAGGGTTCCGCTCTCTGCGCGCTCGAGGACAGCAATGCCGAGCTCGGCCG
CGAGGCGATCCTGAAGCTGATGGAAGCGGTGGACAGCTACATCCCGCAGCCGGAGCGCCCGAAGGACAAGCCGTTCCTGA
TGCCGGTCGAAGACGTGTTCTCGATCTCCGGCCGCGGCACCGTGGTGACCGGCCGCGTCGAGCGCGGCATCATCAAGGTT
GGCGACGAAGTCGAGATCGTCGGCCTGAAGGCCACCGTGAAGACGACGGTCACCGGCGTCGAAATGTTCCGCAAGCTGCT
CGACCAGGGCGAGGCCGGCGACAACATCGGCGCGCTGCTGCGTGGCACCAAGCGTGAAGACGTCGAGCGTGGCCAGGTTC
TCGCCGCGCCGGGCTCGATCACCCCGCACACCAACTTCTCCGGTTCGGTCTACATCCTGAACAAGGAAGAAGGCGGGCGC
CACACGCCGTTCTTCACCAACTATCGCCCGCAGTTCTATTTCCGCACCACCGACGTGACCGGCGTGGTCACGCTGCCGGA
AGGTGTCGAGATGGTGATGCCGGGTGACAACGTGACGGTGAGCGTCGAGCTGATCGCGCCCATCGCGATGGACGAAGGTC
TCCGCTTCGCGATCCGCGAAGGCGGCCGGACGGTTGGTTCGGGCGTCGTCGCGAGCATCACGAAGTAA

Upstream 100 bases:

>100_bases
GGCGAACAAGGCGTTCAGCCACTACCGCTGGTAAGCAACCGTCAGACTCCACCCCATCACGCCAGCACGGCGCTGAAGCA
GATACGTTACGGAGAAACCC

Downstream 100 bases:

>100_bases
CCGCCTGGCTGGTTAGCGAGGCGGCCGCCATCCGGTCTCAACCGGGTGGCGGCCGCTGGATTTGGTTCGGACTAGCGCGC
GGCCCGAATGGGCGGACCGG

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAESGGATFRAYDSIDAAPEERARGITIATAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKMDMADPDLVELVEMEVRDLLSKY
EFPGDDIPIIKGSALCALEDSNAELGREAILKLMEAVDSYIPQPERPKDKPFLMPVEDVFSISGRGTVVTGRVERGIIKV
GDEVEIVGLKATVKTTVTGVEMFRKLLDQGEAGDNIGALLRGTKREDVERGQVLAAPGSITPHTNFSGSVYILNKEEGGR
HTPFFTNYRPQFYFRTTDVTGVVTLPEGVEMVMPGDNVTVSVELIAPIAMDEGLRFAIREGGRTVGSGVVASITK

Sequences:

>Translated_395_residues
MAKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAESGGATFRAYDSIDAAPEERARGITIATAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKMDMADPDLVELVEMEVRDLLSKY
EFPGDDIPIIKGSALCALEDSNAELGREAILKLMEAVDSYIPQPERPKDKPFLMPVEDVFSISGRGTVVTGRVERGIIKV
GDEVEIVGLKATVKTTVTGVEMFRKLLDQGEAGDNIGALLRGTKREDVERGQVLAAPGSITPHTNFSGSVYILNKEEGGR
HTPFFTNYRPQFYFRTTDVTGVVTLPEGVEMVMPGDNVTVSVELIAPIAMDEGLRFAIREGGRTVGSGVVASITK
>Mature_394_residues
AKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAESGGATFRAYDSIDAAPEERARGITIATAHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKMDMADPDLVELVEMEVRDLLSKYE
FPGDDIPIIKGSALCALEDSNAELGREAILKLMEAVDSYIPQPERPKDKPFLMPVEDVFSISGRGTVVTGRVERGIIKVG
DEVEIVGLKATVKTTVTGVEMFRKLLDQGEAGDNIGALLRGTKREDVERGQVLAAPGSITPHTNFSGSVYILNKEEGGRH
TPFFTNYRPQFYFRTTDVTGVVTLPEGVEMVMPGDNVTVSVELIAPIAMDEGLRFAIREGGRTVGSGVVASITK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=392, Percent_Identity=58.6734693877551, Blast_Score=493, Evalue=1e-139,
Organism=Homo sapiens, GI4503475, Length=456, Percent_Identity=30.9210526315789, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=30.6666666666667, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=31.6628701594533, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=31.6628701594533, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI194018522, Length=323, Percent_Identity=30.030959752322, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194097354, Length=323, Percent_Identity=30.030959752322, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI194018520, Length=323, Percent_Identity=30.030959752322, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI46094014, Length=335, Percent_Identity=29.8507462686567, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI54607086, Length=320, Percent_Identity=30, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI157426893, Length=284, Percent_Identity=25.3521126760563, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=73.6040609137056, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=73.6040609137056, Blast_Score=589, Evalue=1e-170,
Organism=Escherichia coli, GI2367247, Length=371, Percent_Identity=30.7277628032345, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI48994988, Length=273, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789108, Length=268, Percent_Identity=30.9701492537313, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788922, Length=153, Percent_Identity=32.6797385620915, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=398, Percent_Identity=53.2663316582915, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=42.7461139896373, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=450, Percent_Identity=31.5555555555556, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17569207, Length=450, Percent_Identity=31.5555555555556, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI32566303, Length=419, Percent_Identity=30.5489260143198, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI115532067, Length=437, Percent_Identity=28.8329519450801, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=437, Percent_Identity=28.8329519450801, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=340, Percent_Identity=30.2941176470588, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=264, Percent_Identity=29.9242424242424, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=39.4557823129252, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=28.2700421940928, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=66.9191919191919, Blast_Score=541, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6325337, Length=442, Percent_Identity=31.447963800905, Blast_Score=175, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6319594, Length=442, Percent_Identity=31.447963800905, Blast_Score=175, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6322937, Length=337, Percent_Identity=27.0029673590504, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=338, Percent_Identity=28.698224852071, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=241, Percent_Identity=28.6307053941909, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320863, Length=285, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=178, Percent_Identity=32.5842696629214, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281363316, Length=390, Percent_Identity=59.7435897435897, Blast_Score=504, Evalue=1e-143,
Organism=Drosophila melanogaster, GI17864358, Length=390, Percent_Identity=59.7435897435897, Blast_Score=504, Evalue=1e-143,
Organism=Drosophila melanogaster, GI19921738, Length=383, Percent_Identity=50.1305483028721, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=30.5122494432071, Blast_Score=174, Evalue=9e-44,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=30.5122494432071, Blast_Score=174, Evalue=9e-44,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=30, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=30, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=30, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=30, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI17137380, Length=341, Percent_Identity=31.0850439882698, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=31.023102310231, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45550900, Length=337, Percent_Identity=28.1899109792285, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24647046, Length=267, Percent_Identity=26.2172284644195, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24647048, Length=356, Percent_Identity=25.8426966292135, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78706572, Length=310, Percent_Identity=27.4193548387097, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28572034, Length=178, Percent_Identity=35.9550561797753, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_ACICJ (A5FZW7)

Other databases:

- EMBL:   CP000697
- RefSeq:   YP_001235068.1
- ProteinModelPortal:   A5FZW7
- SMR:   A5FZW7
- STRING:   A5FZW7
- GeneID:   5160970
- GenomeReviews:   CP000697_GR
- KEGG:   acr:Acry_1948
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- ProtClustDB:   PRK00049
- BioCyc:   ACRY349163:ACRY_1948-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 42629; Mature: 42498

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAESGGATFRAYDSIDAAPEERARG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHCC
ITIATAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPALVVFLNKMDMADPDLVELVEMEVRDLLSKYEFPGDDIPIIKGSALCALED
EEHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEEC
SNAELGREAILKLMEAVDSYIPQPERPKDKPFLMPVEDVFSISGRGTVVTGRVERGIIKV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEECCCCCCEEEE
GDEVEIVGLKATVKTTVTGVEMFRKLLDQGEAGDNIGALLRGTKREDVERGQVLAAPGSI
CCEEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHCCCEEEECCCCC
TPHTNFSGSVYILNKEEGGRHTPFFTNYRPQFYFRTTDVTGVVTLPEGVEMVMPGDNVTV
CCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCEEE
SVELIAPIAMDEGLRFAIREGGRTVGSGVVASITK
EEEEEEEEECCCCCEEEECCCCCEECCCEEEEECC
>Mature Secondary Structure 
AKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAESGGATFRAYDSIDAAPEERARG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHCC
ITIATAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPALVVFLNKMDMADPDLVELVEMEVRDLLSKYEFPGDDIPIIKGSALCALED
EEHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEEC
SNAELGREAILKLMEAVDSYIPQPERPKDKPFLMPVEDVFSISGRGTVVTGRVERGIIKV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEECCCCCCEEEE
GDEVEIVGLKATVKTTVTGVEMFRKLLDQGEAGDNIGALLRGTKREDVERGQVLAAPGSI
CCEEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHCCCEEEECCCCC
TPHTNFSGSVYILNKEEGGRHTPFFTNYRPQFYFRTTDVTGVVTLPEGVEMVMPGDNVTV
CCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCEEE
SVELIAPIAMDEGLRFAIREGGRTVGSGVVASITK
EEEEEEEEECCCCCEEEECCCCCEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA