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The map label for this gene is rpoA

Identifier: 148260915

GI number: 148260915

Start: 2119184

End: 2120221

Strand: Reverse

Name: rpoA

Synonym: Acry_1922

Alternate gene names: 148260915

Gene position: 2120221-2119184 (Counterclockwise)

Preceding gene: 148260916

Following gene: 148260914

Centisome position: 62.56

GC content: 65.9

Gene sequence:

>1038_bases
ATGTTGAGAGACACGCACTTGGCCTTGCAGCGGAACTGGCAGTCGCTGCGCAAGCCGGAAAAACTGGATATCGAACCCGG
CGCGGACGACACGCGCGTCGCGACGATCATTGCCGAGCCGCTCGAGCGCGGCTTTGGCCTGACCCTCGGCAACGCCCTGC
GCCGCGTCCTGCTGTCGTCCCTGCACGGCGCCGCCGTGACCTCGGTGCGGATCGACGGCGTGCTGCACGAATTCTCCTCG
ATCGCCGGCGTGCGCGAGGATGTGACCGACATCGTGCTCAACATCAAGCAGATCGCCGTGCGCATGCATGGCGACGGTCC
GAAGCGGATGACCGTCTCCGCCACCGGCCCCTGCGAGGTGACCGCCGGCATGATCCAGACCGGACACGACATCGAGGTCA
TGAACCCCGAGCTGGTGATCTGCACGCTGGACGATGGCGCGACGCTCGGCATGGAATTCACGGTCGAGTCCGGTCGCGGC
TACGTCCCCGCCGCCGCCAACCGCCCGGAAGACGCGCCGATCGGCCTGATCCCGGTCGACTCGATCTACTCGCCGGTCCG
CCGCGTGTCCTACCGCGTCGAGCCGACCCGCGTCGGCCAGGTGACCGACTATGATCGGCTGATCCTGAACATCGAGACCA
ACGGCGCCGTCACGCCGGAGGATGCCGTGGCCCTCGCCGCCCGCATCCTGCAGGACCAGCTCGGCATGTTCATCAACTTC
GAGGAACCGCAGCGCCTCCGCGCCGAGGAGCCGCGCCCCGAGCTGCCGTTCAACCCGAACCTGCTCCGCAAGGTCGACGA
GCTGGAGCTCTCGGTCCGGTCGGCGAACTGCCTCAAGAACGACAACATCGTCTATATCGGCGACCTCGTGCAGAAATCCG
AACAGGAAATGCTCCGCACGCCGAATTTCGGCCGCAAGTCGCTGAACGAGATCAAGGAAGTCCTCTCCGCCATGGGCCTC
GGCCTCGGCATGGTTGTGCAGGACTGGCCGCCCGAAAACATCGAGGAGCTGGTCAAACGCTCCGACAACCCGTTCTGA

Upstream 100 bases:

>100_bases
GGCGGCCCGGCCCGGAGCGCGCGGCTCCACCGCGCCGCCGGAGAGTCGGCACGTTTTTGGATGGGGTGAGGGTGGCATCG
CCGCCCGACCGGAGGGACTG

Downstream 100 bases:

>100_bases
TCGCCTTTCCACCCAACGCTTTTCGTCTTTACTGATCCGGAGTCACTCCCATGCGTCACGGCCTTGCAGGACGTAAACTC
GGCGTCACCGCGTCCCACCG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MLRDTHLALQRNWQSLRKPEKLDIEPGADDTRVATIIAEPLERGFGLTLGNALRRVLLSSLHGAAVTSVRIDGVLHEFSS
IAGVREDVTDIVLNIKQIAVRMHGDGPKRMTVSATGPCEVTAGMIQTGHDIEVMNPELVICTLDDGATLGMEFTVESGRG
YVPAAANRPEDAPIGLIPVDSIYSPVRRVSYRVEPTRVGQVTDYDRLILNIETNGAVTPEDAVALAARILQDQLGMFINF
EEPQRLRAEEPRPELPFNPNLLRKVDELELSVRSANCLKNDNIVYIGDLVQKSEQEMLRTPNFGRKSLNEIKEVLSAMGL
GLGMVVQDWPPENIEELVKRSDNPF

Sequences:

>Translated_345_residues
MLRDTHLALQRNWQSLRKPEKLDIEPGADDTRVATIIAEPLERGFGLTLGNALRRVLLSSLHGAAVTSVRIDGVLHEFSS
IAGVREDVTDIVLNIKQIAVRMHGDGPKRMTVSATGPCEVTAGMIQTGHDIEVMNPELVICTLDDGATLGMEFTVESGRG
YVPAAANRPEDAPIGLIPVDSIYSPVRRVSYRVEPTRVGQVTDYDRLILNIETNGAVTPEDAVALAARILQDQLGMFINF
EEPQRLRAEEPRPELPFNPNLLRKVDELELSVRSANCLKNDNIVYIGDLVQKSEQEMLRTPNFGRKSLNEIKEVLSAMGL
GLGMVVQDWPPENIEELVKRSDNPF
>Mature_345_residues
MLRDTHLALQRNWQSLRKPEKLDIEPGADDTRVATIIAEPLERGFGLTLGNALRRVLLSSLHGAAVTSVRIDGVLHEFSS
IAGVREDVTDIVLNIKQIAVRMHGDGPKRMTVSATGPCEVTAGMIQTGHDIEVMNPELVICTLDDGATLGMEFTVESGRG
YVPAAANRPEDAPIGLIPVDSIYSPVRRVSYRVEPTRVGQVTDYDRLILNIETNGAVTPEDAVALAARILQDQLGMFINF
EEPQRLRAEEPRPELPFNPNLLRKVDELELSVRSANCLKNDNIVYIGDLVQKSEQEMLRTPNFGRKSLNEIKEVLSAMGL
GLGMVVQDWPPENIEELVKRSDNPF

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=330, Percent_Identity=49.3939393939394, Blast_Score=311, Evalue=6e-86,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_ACICJ (A5FZU1)

Other databases:

- EMBL:   CP000697
- RefSeq:   YP_001235042.1
- ProteinModelPortal:   A5FZU1
- STRING:   A5FZU1
- GeneID:   5160731
- GenomeReviews:   CP000697_GR
- KEGG:   acr:Acry_1922
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   CHIDESA
- ProtClustDB:   PRK05182
- BioCyc:   ACRY349163:ACRY_1922-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 38134; Mature: 38134

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRDTHLALQRNWQSLRKPEKLDIEPGADDTRVATIIAEPLERGFGLTLGNALRRVLLSS
CCCCHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LHGAAVTSVRIDGVLHEFSSIAGVREDVTDIVLNIKQIAVRMHGDGPKRMTVSATGPCEV
HCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCHH
TAGMIQTGHDIEVMNPELVICTLDDGATLGMEFTVESGRGYVPAAANRPEDAPIGLIPVD
HHHHHCCCCCEEEECCEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHH
SIYSPVRRVSYRVEPTRVGQVTDYDRLILNIETNGAVTPEDAVALAARILQDQLGMFINF
HHHHHHHHHHEECCCHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCEEEEC
EEPQRLRAEEPRPELPFNPNLLRKVDELELSVRSANCLKNDNIVYIGDLVQKSEQEMLRT
CCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHC
PNFGRKSLNEIKEVLSAMGLGLGMVVQDWPPENIEELVKRSDNPF
CCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MLRDTHLALQRNWQSLRKPEKLDIEPGADDTRVATIIAEPLERGFGLTLGNALRRVLLSS
CCCCHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LHGAAVTSVRIDGVLHEFSSIAGVREDVTDIVLNIKQIAVRMHGDGPKRMTVSATGPCEV
HCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCHH
TAGMIQTGHDIEVMNPELVICTLDDGATLGMEFTVESGRGYVPAAANRPEDAPIGLIPVD
HHHHHCCCCCEEEECCEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHH
SIYSPVRRVSYRVEPTRVGQVTDYDRLILNIETNGAVTPEDAVALAARILQDQLGMFINF
HHHHHHHHHHEECCCHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCEEEEC
EEPQRLRAEEPRPELPFNPNLLRKVDELELSVRSANCLKNDNIVYIGDLVQKSEQEMLRT
CCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHC
PNFGRKSLNEIKEVLSAMGLGLGMVVQDWPPENIEELVKRSDNPF
CCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA