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The map label for this gene is pcaJ [H]

Identifier: 148260839

GI number: 148260839

Start: 2035778

End: 2036434

Strand: Reverse

Name: pcaJ [H]

Synonym: Acry_1845

Alternate gene names: 148260839

Gene position: 2036434-2035778 (Counterclockwise)

Preceding gene: 148260840

Following gene: 148260838

Centisome position: 60.09

GC content: 71.99

Gene sequence:

>657_bases
ATGGAGGGGACGGTGATGCGGCGGGGCTGGTCGCGCGCGGAGATGGCCGCGCGGGTGGCGGCGGATATTCCCGAGGGCTG
GGTGGTCAATCTCGGCATCGGGCTGCCGACGCAGGTGGCGAACCATGTGCCGGCGGAGCGGGAGGTGATCTTCCATTCCG
AGAACGGCATCCTGGGGATGGGGCCGGCGCCGGACGCGGGGGCGGAGGACCCGTTCCTGATCAACGCGGGCAAGCAGCCG
GTGACGCTGCGTCCGGGGGCGTCGATCGTCGGGCAGGCGGACAGTTTCGCGATCATCCGCGGGCGGCACCTCGATCTGTG
CGTGCTCGGCGGGTATCAGGTGGCGGCGAACGGCGATCTCGCCAACTGGTCGGTCGGGCGCAAGGGCGTGGCGCCGGCGG
TGGGCGGGGCGATGGACCTCGGCGCTGGGGCGGCGCGGGTCTGGGTGCTGATGGAGCACTGCACGAGGGAGGGCGAGCCG
CGGCTGGTGGAGCGCTGCACCTATCCGCTGACCGCGCTGGGCTCGATCACCCGCGTCTATACCGATCTTGCCGTCATCGA
CATCACGCCGGCGGGGTTCGTGCTGCGCGACGCGGCACCAGGGGTCACGCCGGAACAGGTGCGCAGCCTGACCGCCGCCC
CTCTCGCCATCGGGTGA

Upstream 100 bases:

>100_bases
TCGAGCTCGGCGCGCTGGATCCGGACCGGATCGTGACGCCCGGCGTGTTCGTCGACCGGGTGGTGCTGGTGCCGGAGCCG
ATGGAAGGGGAGGCGGCGTG

Downstream 100 bases:

>100_bases
CGATCGCCGGCATCAGGGCCGCCGACCGGTCATCCGCCGGCGGGCGAGGGGCGTTCCGGCCCAGCCTCGGTGGGGGGACG
GCGCCGGTATGCGCCAGCCA

Product: 3-oxoacid CoA-transferase subunit B

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MEGTVMRRGWSRAEMAARVAADIPEGWVVNLGIGLPTQVANHVPAEREVIFHSENGILGMGPAPDAGAEDPFLINAGKQP
VTLRPGASIVGQADSFAIIRGRHLDLCVLGGYQVAANGDLANWSVGRKGVAPAVGGAMDLGAGAARVWVLMEHCTREGEP
RLVERCTYPLTALGSITRVYTDLAVIDITPAGFVLRDAAPGVTPEQVRSLTAAPLAIG

Sequences:

>Translated_218_residues
MEGTVMRRGWSRAEMAARVAADIPEGWVVNLGIGLPTQVANHVPAEREVIFHSENGILGMGPAPDAGAEDPFLINAGKQP
VTLRPGASIVGQADSFAIIRGRHLDLCVLGGYQVAANGDLANWSVGRKGVAPAVGGAMDLGAGAARVWVLMEHCTREGEP
RLVERCTYPLTALGSITRVYTDLAVIDITPAGFVLRDAAPGVTPEQVRSLTAAPLAIG
>Mature_218_residues
MEGTVMRRGWSRAEMAARVAADIPEGWVVNLGIGLPTQVANHVPAEREVIFHSENGILGMGPAPDAGAEDPFLINAGKQP
VTLRPGASIVGQADSFAIIRGRHLDLCVLGGYQVAANGDLANWSVGRKGVAPAVGGAMDLGAGAARVWVLMEHCTREGEP
RLVERCTYPLTALGSITRVYTDLAVIDITPAGFVLRDAAPGVTPEQVRSLTAAPLAIG

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI11545841, Length=209, Percent_Identity=41.6267942583732, Blast_Score=162, Evalue=1e-40,
Organism=Homo sapiens, GI4557817, Length=208, Percent_Identity=40.8653846153846, Blast_Score=151, Evalue=3e-37,
Organism=Escherichia coli, GI1788552, Length=207, Percent_Identity=44.4444444444444, Blast_Score=167, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17531517, Length=211, Percent_Identity=48.3412322274881, Blast_Score=191, Evalue=3e-49,
Organism=Drosophila melanogaster, GI21358325, Length=208, Percent_Identity=42.7884615384615, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24655794, Length=208, Percent_Identity=42.7884615384615, Blast_Score=168, Evalue=3e-42,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 22765; Mature: 22765

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGTVMRRGWSRAEMAARVAADIPEGWVVNLGIGLPTQVANHVPAEREVIFHSENGILGM
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCCCEEEEECCCCEEEC
GPAPDAGAEDPFLINAGKQPVTLRPGASIVGQADSFAIIRGRHLDLCVLGGYQVAANGDL
CCCCCCCCCCCEEEECCCCCEEECCCCCEEECCCCEEEEECCCEEEEEECCEEEEECCCC
ANWSVGRKGVAPAVGGAMDLGAGAARVWVLMEHCTREGEPRLVERCTYPLTALGSITRVY
CCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHH
TDLAVIDITPAGFVLRDAAPGVTPEQVRSLTAAPLAIG
HCEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCEECC
>Mature Secondary Structure
MEGTVMRRGWSRAEMAARVAADIPEGWVVNLGIGLPTQVANHVPAEREVIFHSENGILGM
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCCCEEEEECCCCEEEC
GPAPDAGAEDPFLINAGKQPVTLRPGASIVGQADSFAIIRGRHLDLCVLGGYQVAANGDL
CCCCCCCCCCCEEEECCCCCEEECCCCCEEECCCCEEEEECCCEEEEEECCEEEEECCCC
ANWSVGRKGVAPAVGGAMDLGAGAARVWVLMEHCTREGEPRLVERCTYPLTALGSITRVY
CCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHH
TDLAVIDITPAGFVLRDAAPGVTPEQVRSLTAAPLAIG
HCEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]