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The map label for this gene is gltD [H]

Identifier: 148260664

GI number: 148260664

Start: 1841252

End: 1842703

Strand: Reverse

Name: gltD [H]

Synonym: Acry_1667

Alternate gene names: 148260664

Gene position: 1842703-1841252 (Counterclockwise)

Preceding gene: 148260665

Following gene: 148260663

Centisome position: 54.37

GC content: 69.83

Gene sequence:

>1452_bases
ATGACCGAGCACATGCAGCAATTCGTGCGTCTTGCGCAGCAGCCGCCGGAAAAGCGCGCCGCCGTCGAACGCCGCGAGGA
TTTCGGCGAGATCTATCGCGATTTCGACCCGAAGGACGCGGCGGCCCAGGCCAGCCGGTGCAGCCAGTGCGGGATTCCGT
TCTGTCAGGTGCATTGCCCGCTCGGCAACAACATCCCCGACTGGCTGAAGCTCTCGGCCGAGGGGCGGCTCGAGGAAGCC
TACGAGATTTCCGCCGCCACCAACACGTTCCCCGAAATCTGCGGCCGCATCTGCCCGCAGGACCGGCTCTGCGAGGGCAA
TTGCGTCATCGAGAAGGGGTTCGAGAGCGTGACCATCGGCGCCGTCGAGCGCTACATCACCGACACCGCCTTCGAGAATG
GCTGGGTCCGGCCGATCCGCCCGCGGCGCGAGCGCGCGCAGAGTGTCGGCATCATCGGCGCCGGGCCGGCCGGCCTCGCC
GCCGCCGAGGCGCTGCGGCGCAAGGGGTATCAGGTGCATGTCCATGACCGGCACGACCGGGTGGGCGGGCTGCTGATCTA
CGGCATTCCCGGCTTCAAGCTGGAGAAGGAGATCGTCGCCCGGCGGCGCGCCTGGCTCGAGGACGGCGGCATCCATTTCC
ATCTGAACGAGGATATCGGCGGCGCCCTGAGCTTTGCCGAGCTGCGCGCGCGCTACGACGCGGTGCTGGTCGCCACCGGC
GTCTACAAGGCGCGCGAGATCGACGTGCCGGGCAGCCGGCTCGACGGCGTGGTGCCGGCGCTCGACTATCTCATCGCCTC
GAACCGCAAGGGGCTGGGCGATGCGGTGCCGGACTATGATTCGGGTGCCTTGAACGCCGCCGGCAAGCGCGTCGTCGTCA
TCGGCGGCGGCGACACGGCGATGGACTGTGTCCGCACCGCGATCCGCCAGGACGCCGCCGCCGTCACCTGCCTCTATCGG
CGCGACCGCGCCAACATGCCCGGCTCGGCGCGCGAGGTCGCGAACGCGATCGACGAGGGTGTCGAGTTCACCTGGCTCTC
CGCGCCGGAGGCCTTCGTCGGCGCCGGGCGGGTCAGTGCGGTGAAGGCGGTGCGGATGCATCTCGGCCTCGCCGATGCCT
CGGGCCGCCAGACGGTCGAGCCCATCGCGGGTTCGGAATTCACCATCGAGGCCGATCTCGTCATAAAGGCGCTCGGCTTC
GACCCCGAGGATCTGCCCGGCGCCTTCAACGAGCCGGGCCTCACGCTGACCCGCTGGGGCACGCTGAAGGTCGATTCGAA
GAGTTTCATGACCGCGCTGCCCGGCGTGTTCGCCGCCGGCGACATCGTGCGCGGCGCCAGCCTCGTCGTCTGGGCGATCA
AGGACGGCCGCGATGCCGCCGACGCCATGCACGCCTATCTCGAAACCGCCGCCGCGCGCCCCGACGGCGCGCTCGCCACC
GCCGCGGAGTGA

Upstream 100 bases:

>100_bases
GGCAGTTGATATGACTTAACATCGAATTATTGCTGGCAATCTCCGTTCCCGTGAGCTAGATACTGCCGGAAACAGCTATC
CACGGAACGGAGTTGTGCCG

Downstream 100 bases:

>100_bases
TGACCATGACCCATGAAACCGCCTCCGAATTTCTCGCCGCCTGGCAGCGCAACACCACGGCCCTCGCCGGCAGCTACGAT
CCGGCGCAGGAACACGATGC

Product: putative oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTEHMQQFVRLAQQPPEKRAAVERREDFGEIYRDFDPKDAAAQASRCSQCGIPFCQVHCPLGNNIPDWLKLSAEGRLEEA
YEISAATNTFPEICGRICPQDRLCEGNCVIEKGFESVTIGAVERYITDTAFENGWVRPIRPRRERAQSVGIIGAGPAGLA
AAEALRRKGYQVHVHDRHDRVGGLLIYGIPGFKLEKEIVARRRAWLEDGGIHFHLNEDIGGALSFAELRARYDAVLVATG
VYKAREIDVPGSRLDGVVPALDYLIASNRKGLGDAVPDYDSGALNAAGKRVVVIGGGDTAMDCVRTAIRQDAAAVTCLYR
RDRANMPGSAREVANAIDEGVEFTWLSAPEAFVGAGRVSAVKAVRMHLGLADASGRQTVEPIAGSEFTIEADLVIKALGF
DPEDLPGAFNEPGLTLTRWGTLKVDSKSFMTALPGVFAAGDIVRGASLVVWAIKDGRDAADAMHAYLETAAARPDGALAT
AAE

Sequences:

>Translated_483_residues
MTEHMQQFVRLAQQPPEKRAAVERREDFGEIYRDFDPKDAAAQASRCSQCGIPFCQVHCPLGNNIPDWLKLSAEGRLEEA
YEISAATNTFPEICGRICPQDRLCEGNCVIEKGFESVTIGAVERYITDTAFENGWVRPIRPRRERAQSVGIIGAGPAGLA
AAEALRRKGYQVHVHDRHDRVGGLLIYGIPGFKLEKEIVARRRAWLEDGGIHFHLNEDIGGALSFAELRARYDAVLVATG
VYKAREIDVPGSRLDGVVPALDYLIASNRKGLGDAVPDYDSGALNAAGKRVVVIGGGDTAMDCVRTAIRQDAAAVTCLYR
RDRANMPGSAREVANAIDEGVEFTWLSAPEAFVGAGRVSAVKAVRMHLGLADASGRQTVEPIAGSEFTIEADLVIKALGF
DPEDLPGAFNEPGLTLTRWGTLKVDSKSFMTALPGVFAAGDIVRGASLVVWAIKDGRDAADAMHAYLETAAARPDGALAT
AAE
>Mature_482_residues
TEHMQQFVRLAQQPPEKRAAVERREDFGEIYRDFDPKDAAAQASRCSQCGIPFCQVHCPLGNNIPDWLKLSAEGRLEEAY
EISAATNTFPEICGRICPQDRLCEGNCVIEKGFESVTIGAVERYITDTAFENGWVRPIRPRRERAQSVGIIGAGPAGLAA
AEALRRKGYQVHVHDRHDRVGGLLIYGIPGFKLEKEIVARRRAWLEDGGIHFHLNEDIGGALSFAELRARYDAVLVATGV
YKAREIDVPGSRLDGVVPALDYLIASNRKGLGDAVPDYDSGALNAAGKRVVVIGGGDTAMDCVRTAIRQDAAAVTCLYRR
DRANMPGSAREVANAIDEGVEFTWLSAPEAFVGAGRVSAVKAVRMHLGLADASGRQTVEPIAGSEFTIEADLVIKALGFD
PEDLPGAFNEPGLTLTRWGTLKVDSKSFMTALPGVFAAGDIVRGASLVVWAIKDGRDAADAMHAYLETAAARPDGALATA
AE

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=461, Percent_Identity=26.4642082429501, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1789606, Length=474, Percent_Identity=47.2573839662447, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1788811, Length=474, Percent_Identity=45.3586497890295, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI87082180, Length=463, Percent_Identity=44.9244060475162, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1788468, Length=444, Percent_Identity=30.4054054054054, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1789243, Length=424, Percent_Identity=31.6037735849057, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17570289, Length=516, Percent_Identity=35.4651162790698, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI71984108, Length=467, Percent_Identity=28.4796573875803, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17543860, Length=161, Percent_Identity=33.5403726708075, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=469, Percent_Identity=37.3134328358209, Blast_Score=263, Evalue=6e-71,
Organism=Drosophila melanogaster, GI28574881, Length=494, Percent_Identity=39.2712550607287, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24665539, Length=494, Percent_Identity=39.2712550607287, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24665547, Length=502, Percent_Identity=38.8446215139442, Blast_Score=302, Evalue=4e-82,
Organism=Drosophila melanogaster, GI24665543, Length=502, Percent_Identity=38.8446215139442, Blast_Score=302, Evalue=4e-82,
Organism=Drosophila melanogaster, GI24640763, Length=478, Percent_Identity=26.9874476987448, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI18858217, Length=478, Percent_Identity=26.9874476987448, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52043; Mature: 51912

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHMQQFVRLAQQPPEKRAAVERREDFGEIYRDFDPKDAAAQASRCSQCGIPFCQVHCP
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECC
LGNNIPDWLKLSAEGRLEEAYEISAATNTFPEICGRICPQDRLCEGNCVIEKGFESVTIG
CCCCCHHHHEECCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEECCCCEEEHH
AVERYITDTAFENGWVRPIRPRRERAQSVGIIGAGPAGLAAAEALRRKGYQVHVHDRHDR
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEECCCHHHHHHHHHHHCCCEEEEECCCCC
VGGLLIYGIPGFKLEKEIVARRRAWLEDGGIHFHLNEDIGGALSFAELRARYDAVLVATG
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCEEEEEEC
VYKAREIDVPGSRLDGVVPALDYLIASNRKGLGDAVPDYDSGALNAAGKRVVVIGGGDTA
CCEEEEECCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHH
MDCVRTAIRQDAAAVTCLYRRDRANMPGSAREVANAIDEGVEFTWLSAPEAFVGAGRVSA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHCCCHHHH
VKAVRMHLGLADASGRQTVEPIAGSEFTIEADLVIKALGFDPEDLPGAFNEPGLTLTRWG
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHEEEEECCCCCHHCCCCCCCCCEEEEECC
TLKVDSKSFMTALPGVFAAGDIVRGASLVVWAIKDGRDAADAMHAYLETAAARPDGALAT
EEEECCHHHHHHHCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCC
AAE
CCC
>Mature Secondary Structure 
TEHMQQFVRLAQQPPEKRAAVERREDFGEIYRDFDPKDAAAQASRCSQCGIPFCQVHCP
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECC
LGNNIPDWLKLSAEGRLEEAYEISAATNTFPEICGRICPQDRLCEGNCVIEKGFESVTIG
CCCCCHHHHEECCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEECCCCEEEHH
AVERYITDTAFENGWVRPIRPRRERAQSVGIIGAGPAGLAAAEALRRKGYQVHVHDRHDR
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEECCCHHHHHHHHHHHCCCEEEEECCCCC
VGGLLIYGIPGFKLEKEIVARRRAWLEDGGIHFHLNEDIGGALSFAELRARYDAVLVATG
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCEEEEEEC
VYKAREIDVPGSRLDGVVPALDYLIASNRKGLGDAVPDYDSGALNAAGKRVVVIGGGDTA
CCEEEEECCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHH
MDCVRTAIRQDAAAVTCLYRRDRANMPGSAREVANAIDEGVEFTWLSAPEAFVGAGRVSA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHCCCHHHH
VKAVRMHLGLADASGRQTVEPIAGSEFTIEADLVIKALGFDPEDLPGAFNEPGLTLTRWG
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHEEEEECCCCCHHCCCCCCCCCEEEEECC
TLKVDSKSFMTALPGVFAAGDIVRGASLVVWAIKDGRDAADAMHAYLETAAARPDGALAT
EEEECCHHHHHHHCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCC
AAE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8428988; 2198943 [H]