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The map label for this gene is sucB [H]

Identifier: 148260620

GI number: 148260620

Start: 1790583

End: 1791815

Strand: Reverse

Name: sucB [H]

Synonym: Acry_1622

Alternate gene names: 148260620

Gene position: 1791815-1790583 (Counterclockwise)

Preceding gene: 148260621

Following gene: 148260619

Centisome position: 52.87

GC content: 69.1

Gene sequence:

>1233_bases
ATGAGCACCGAAATCAAGGTTCCCATCCTCGGCGAGAGCGTGACCACCGCGACCGTCGCGCGCTGGATCAGGAAAGTGGG
CGAGACCGTCGCCCAGGACGAGCCGATCGTCGAGCTGGAGACCGACAAGGTCACCGTCGAGGTCAATGCGCCCGAGGCCG
GCACGATCGAGGCGATCGCCGCCGATGAGGGGGCCGAGGTCGAGGTCGGCGCCCTGCTGGGCACGATCGGCGCCGGCACC
GGTGCCGCCGCCGCGCCGAAAAAGGCCGAGCCCGCGGCGGAGGCCCGCCCGGCCGCCAATCCGCAGCCCGGCGTCAACCC
GCCGCCGCCGCCCTCCGGCCCGGTCGCCCGCACCGGCCACGCGCCGATGCCCGCCGCGCAGAAGATGATGACCGAGCAGA
AGGTGGATGCCGCGCAGGTCGAGGGCACCGGCAAGGACGGCCGCATCACCAAGGGCGACGTGCTCGCCTTCATCGAGAAG
CCGGCCGCCGCCCCGGCGCCGGCAGCACCCAGGGCCCAGCGCGCCGCCGATGCGCGCGAGGAACGGGTGAAGATGACCCG
CCTGCGCAAGACCATCGCCGCCCGGCTGAAGGAGGCGCAGAACACCGCCGCCATGCTCACCACCTTCAACGAGGTGGACA
TGGGCCCGGTGATGGCGCTCCGCGCCGAATACAAGGACGTGTTCGAGAAGAAGCACAAGGGCATTCGCCTCGGCTTCATG
AGCTTCTTCGTTCGCGCCTGCATCGCCGCGCTGCGCGAGTTCCCGGCGGTCAACGCCGAGATCGACGGCGACGAGATCGT
CTACAAGAACTTCGTCAACATGGGCATCGCCGTCTCCAGCCCCTCGGGCCTGGTCGTGCCGGTGCTGCGCGACGCCGACC
AGATGAACTTCCCGCAGATCGAGGGGGCGATCGCCGATTTCGGCAAGCGCGCCCGCGACGGCGCGCTGAAGCTCGACGAG
CTTTCGGGCGGCTCCTTCTCCATCACCAATGGCGGCGTGTTCGGCTCGCTGATGTCGACCCCGATCATCAATCCGCCGCA
ATCGGCCATTCTCGGCATGCACAAGATCCAGGACCGGCCGATGGCGGTCGGCGGCAAGGTCGAGATCCGGCCGATGATGT
ATCTCGCGCTGTCCTACGACCACCGCATCATCGACGGGCGCGAGGCGGTCTCCTTCCTCGTCCGGGTGAAGGAGAGCATC
GAGGATCCGCGCCGCCTGCTGCTGGACATCTGA

Upstream 100 bases:

>100_bases
CGACGGGGCTTGCGAAGGTTCACGCCGCCGAACAGGCGGCCCTGGTCAGGGAAGCGCTCGGCGTCTGAGGCGCGGGCAGG
AACAGCAAAAGGCAGGAAGC

Downstream 100 bases:

>100_bases
GGAGCGCATCATGGCCGATCAGTTCGACGTCATCGTCATCGGGGCCGGTCCGGGCGGCTATGTCTGCGCCATCCGCGCCG
CCCAGCTCGGCATGAAGGTC

Product: 2-oxoglutarate dehydrogenase, E2 subunit, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSTEIKVPILGESVTTATVARWIRKVGETVAQDEPIVELETDKVTVEVNAPEAGTIEAIAADEGAEVEVGALLGTIGAGT
GAAAAPKKAEPAAEARPAANPQPGVNPPPPPSGPVARTGHAPMPAAQKMMTEQKVDAAQVEGTGKDGRITKGDVLAFIEK
PAAAPAPAAPRAQRAADAREERVKMTRLRKTIAARLKEAQNTAAMLTTFNEVDMGPVMALRAEYKDVFEKKHKGIRLGFM
SFFVRACIAALREFPAVNAEIDGDEIVYKNFVNMGIAVSSPSGLVVPVLRDADQMNFPQIEGAIADFGKRARDGALKLDE
LSGGSFSITNGGVFGSLMSTPIINPPQSAILGMHKIQDRPMAVGGKVEIRPMMYLALSYDHRIIDGREAVSFLVRVKESI
EDPRRLLLDI

Sequences:

>Translated_410_residues
MSTEIKVPILGESVTTATVARWIRKVGETVAQDEPIVELETDKVTVEVNAPEAGTIEAIAADEGAEVEVGALLGTIGAGT
GAAAAPKKAEPAAEARPAANPQPGVNPPPPPSGPVARTGHAPMPAAQKMMTEQKVDAAQVEGTGKDGRITKGDVLAFIEK
PAAAPAPAAPRAQRAADAREERVKMTRLRKTIAARLKEAQNTAAMLTTFNEVDMGPVMALRAEYKDVFEKKHKGIRLGFM
SFFVRACIAALREFPAVNAEIDGDEIVYKNFVNMGIAVSSPSGLVVPVLRDADQMNFPQIEGAIADFGKRARDGALKLDE
LSGGSFSITNGGVFGSLMSTPIINPPQSAILGMHKIQDRPMAVGGKVEIRPMMYLALSYDHRIIDGREAVSFLVRVKESI
EDPRRLLLDI
>Mature_409_residues
STEIKVPILGESVTTATVARWIRKVGETVAQDEPIVELETDKVTVEVNAPEAGTIEAIAADEGAEVEVGALLGTIGAGTG
AAAAPKKAEPAAEARPAANPQPGVNPPPPPSGPVARTGHAPMPAAQKMMTEQKVDAAQVEGTGKDGRITKGDVLAFIEKP
AAAPAPAAPRAQRAADAREERVKMTRLRKTIAARLKEAQNTAAMLTTFNEVDMGPVMALRAEYKDVFEKKHKGIRLGFMS
FFVRACIAALREFPAVNAEIDGDEIVYKNFVNMGIAVSSPSGLVVPVLRDADQMNFPQIEGAIADFGKRARDGALKLDEL
SGGSFSITNGGVFGSLMSTPIINPPQSAILGMHKIQDRPMAVGGKVEIRPMMYLALSYDHRIIDGREAVSFLVRVKESIE
DPRRLLLDI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=253, Percent_Identity=57.7075098814229, Blast_Score=304, Evalue=9e-83,
Organism=Homo sapiens, GI31711992, Length=442, Percent_Identity=28.2805429864253, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI203098753, Length=466, Percent_Identity=27.4678111587983, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI203098816, Length=466, Percent_Identity=27.4678111587983, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=27.6497695852535, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI260898739, Length=166, Percent_Identity=33.7349397590361, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1786946, Length=413, Percent_Identity=51.3317191283293, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1786305, Length=426, Percent_Identity=30.2816901408451, Blast_Score=175, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=425, Percent_Identity=44.7058823529412, Blast_Score=332, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI17560088, Length=434, Percent_Identity=29.9539170506912, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=421, Percent_Identity=26.3657957244656, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=29.7385620915033, Blast_Score=128, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=409, Percent_Identity=44.0097799511002, Blast_Score=326, Evalue=4e-90,
Organism=Saccharomyces cerevisiae, GI6324258, Length=453, Percent_Identity=25.8278145695364, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24645909, Length=237, Percent_Identity=57.8059071729958, Blast_Score=285, Evalue=3e-77,
Organism=Drosophila melanogaster, GI18859875, Length=441, Percent_Identity=26.984126984127, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI20129315, Length=289, Percent_Identity=28.0276816608997, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24582497, Length=289, Percent_Identity=30.4498269896194, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43617; Mature: 43486

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEIKVPILGESVTTATVARWIRKVGETVAQDEPIVELETDKVTVEVNAPEAGTIEAIA
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCEEEEE
ADEGAEVEVGALLGTIGAGTGAAAAPKKAEPAAEARPAANPQPGVNPPPPPSGPVARTGH
CCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCC
APMPAAQKMMTEQKVDAAQVEGTGKDGRITKGDVLAFIEKPAAAPAPAAPRAQRAADARE
CCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
ERVKMTRLRKTIAARLKEAQNTAAMLTTFNEVDMGPVMALRAEYKDVFEKKHKGIRLGFM
HHHHHHHHHHHHHHHHHHHHCHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEHHH
SFFVRACIAALREFPAVNAEIDGDEIVYKNFVNMGIAVSSPSGLVVPVLRDADQMNFPQI
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECCCCEEEEEECCCCCCCCCHH
EGAIADFGKRARDGALKLDELSGGSFSITNGGVFGSLMSTPIINPPQSAILGMHKIQDRP
HHHHHHHHHHHCCCCEEEEECCCCEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCC
MAVGGKVEIRPMMYLALSYDHRIIDGREAVSFLVRVKESIEDPRRLLLDI
EECCCEEEECEEEEEEEECCCEEECHHHHHHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
STEIKVPILGESVTTATVARWIRKVGETVAQDEPIVELETDKVTVEVNAPEAGTIEAIA
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCEEEEE
ADEGAEVEVGALLGTIGAGTGAAAAPKKAEPAAEARPAANPQPGVNPPPPPSGPVARTGH
CCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCC
APMPAAQKMMTEQKVDAAQVEGTGKDGRITKGDVLAFIEKPAAAPAPAAPRAQRAADARE
CCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
ERVKMTRLRKTIAARLKEAQNTAAMLTTFNEVDMGPVMALRAEYKDVFEKKHKGIRLGFM
HHHHHHHHHHHHHHHHHHHHCHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEHHH
SFFVRACIAALREFPAVNAEIDGDEIVYKNFVNMGIAVSSPSGLVVPVLRDADQMNFPQI
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECCCCEEEEEECCCCCCCCCHH
EGAIADFGKRARDGALKLDELSGGSFSITNGGVFGSLMSTPIINPPQSAILGMHKIQDRP
HHHHHHHHHHHCCCCEEEEECCCCEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCC
MAVGGKVEIRPMMYLALSYDHRIIDGREAVSFLVRVKESIEDPRRLLLDI
EECCCEEEECEEEEEEEECCCEEECHHHHHHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA