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The map label for this gene is nudG [H]

Identifier: 148260547

GI number: 148260547

Start: 1707759

End: 1708763

Strand: Reverse

Name: nudG [H]

Synonym: Acry_1547

Alternate gene names: 148260547

Gene position: 1708763-1707759 (Counterclockwise)

Preceding gene: 148260550

Following gene: 148260546

Centisome position: 50.42

GC content: 72.94

Gene sequence:

>1005_bases
GTGCGGGGGTTTTGCGTGCCGGATAGCGCCACCAGGGAAACCGTCGGAGCGGCCGTGCTGCGGCCGCTTCAGCAGGAGGA
TGCCGCCCGCTTCCACCAGCTCATCAACGACTGGGACATTTGCCGCCGGCTGCCGGAAGCGCCGTTTCCCTACCCGCTGG
AGACCGCCGAGGCCTGGGTGGCCGCGGCGGTGGCCGACCGTGCCGCCGGGCGCGCCCACGAATTCGCGATTCTCGATGCG
GATGGCAGCATGATCGGCAGCGCCGGGCTGCGCCTCGATGCCGCGCGGGGCAGCGCTGCGCTGGGATACTGGATCGGCCG
CCGGTTCTGGCGCCGCGGCCATGCGGGGGCGGCCGTCGCCGAGCTGCTGCACTGGGGGTTCGCCGAGCTGCCGATCGCGA
CGATCGAGGCGACGGTGGCCGCCGACAACGCGGCGTCGATCGCGCTGCTCGAACGCGCCGGGTTCAGCGCGACGGGGCGC
GGCCATGCGAAGTTCATCGGCCAGCCCGGCGCGAGCCTGCCGGTGATGCGTTACGCGATCACCCGGGAGGCGCTGGCGCT
GCGCCACCTCGCCGCGCCCGAGCCGGTGGCCGCGCCCGCCGCCGCGAAGCCGCTGGTGCTCGTGGCCGCCTGCGCGCTGG
TCGATATCGAGGGGCGGATCCTGCTCGCCCGCCGCCCGCCCGGCAAGAAGATGGCCGGGCTGTGGGAGTTTCCGGGCGGC
AAGCTCGCACCCGGCGAAACCCCCGAACGGGCGCTGGTGCGGGAGATGGAGGAGGAACTCGGCATTTTGCTCCGCGAGGA
GGATGTCGCCCCGTTCGCCTTCGCCTCGCACGCCTATGACCAGTTTCATCTGCTGATGCCGCTCTACCTCGCCCGCCGCT
GGAGCGGCACGCCCGAGCCGCGCGAGGGCCAGGCGCTGGCTTGGGTGCCGCCGGACCGGCTCGACGAATATCCGATGCCG
CCGGCCGACCGACCGCTGCTGCCGCTGCTGCGCGATTTTCTGTGA

Upstream 100 bases:

>100_bases
GGGAGAATTGCGCGCCCGCGGCCGGCAAATCAACCGTCAAACCGTGGATCGCCCTTTCGGCCGCGCGCCGGACAAGCTAT
CCCGGTGCGGCCAGGAGACA

Downstream 100 bases:

>100_bases
TCCGGGAGACCGACATGCCCAGCAATCCCACCGCCGCCATCCTGATCATCGGCAACGAGATCCTCTCCGGCCGGACGCAG
GACGTGAACACGAAATTCAT

Product: NUDIX hydrolase

Products: CMP; diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MRGFCVPDSATRETVGAAVLRPLQQEDAARFHQLINDWDICRRLPEAPFPYPLETAEAWVAAAVADRAAGRAHEFAILDA
DGSMIGSAGLRLDAARGSAALGYWIGRRFWRRGHAGAAVAELLHWGFAELPIATIEATVAADNAASIALLERAGFSATGR
GHAKFIGQPGASLPVMRYAITREALALRHLAAPEPVAAPAAAKPLVLVAACALVDIEGRILLARRPPGKKMAGLWEFPGG
KLAPGETPERALVREMEEELGILLREEDVAPFAFASHAYDQFHLLMPLYLARRWSGTPEPREGQALAWVPPDRLDEYPMP
PADRPLLPLLRDFL

Sequences:

>Translated_334_residues
MRGFCVPDSATRETVGAAVLRPLQQEDAARFHQLINDWDICRRLPEAPFPYPLETAEAWVAAAVADRAAGRAHEFAILDA
DGSMIGSAGLRLDAARGSAALGYWIGRRFWRRGHAGAAVAELLHWGFAELPIATIEATVAADNAASIALLERAGFSATGR
GHAKFIGQPGASLPVMRYAITREALALRHLAAPEPVAAPAAAKPLVLVAACALVDIEGRILLARRPPGKKMAGLWEFPGG
KLAPGETPERALVREMEEELGILLREEDVAPFAFASHAYDQFHLLMPLYLARRWSGTPEPREGQALAWVPPDRLDEYPMP
PADRPLLPLLRDFL
>Mature_334_residues
MRGFCVPDSATRETVGAAVLRPLQQEDAARFHQLINDWDICRRLPEAPFPYPLETAEAWVAAAVADRAAGRAHEFAILDA
DGSMIGSAGLRLDAARGSAALGYWIGRRFWRRGHAGAAVAELLHWGFAELPIATIEATVAADNAASIALLERAGFSATGR
GHAKFIGQPGASLPVMRYAITREALALRHLAAPEPVAAPAAAKPLVLVAACALVDIEGRILLARRPPGKKMAGLWEFPGG
KLAPGETPERALVREMEEELGILLREEDVAPFAFASHAYDQFHLLMPLYLARRWSGTPEPREGQALAWVPPDRLDEYPMP
PADRPLLPLLRDFL

Specific function: Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=124, Percent_Identity=41.1290322580645, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786288, Length=124, Percent_Identity=33.8709677419355, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 36230; Mature: 36230

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGFCVPDSATRETVGAAVLRPLQQEDAARFHQLINDWDICRRLPEAPFPYPLETAEAWV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
AAAVADRAAGRAHEFAILDADGSMIGSAGLRLDAARGSAALGYWIGRRFWRRGHAGAAVA
HHHHHHHHCCCCEEEEEEECCCCEECCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHH
ELLHWGFAELPIATIEATVAADNAASIALLERAGFSATGRGHAKFIGQPGASLPVMRYAI
HHHHCCHHHCCHHHHEEEEECCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHH
TREALALRHLAAPEPVAAPAAAKPLVLVAACALVDIEGRILLARRPPGKKMAGLWEFPGG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEECCCEEEEEECCCCCCCCCEEECCCC
KLAPGETPERALVREMEEELGILLREEDVAPFAFASHAYDQFHLLMPLYLARRWSGTPEP
CCCCCCCHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
REGQALAWVPPDRLDEYPMPPADRPLLPLLRDFL
CCCCEEEECCCHHCCCCCCCCCCCCHHHHHHHHC
>Mature Secondary Structure
MRGFCVPDSATRETVGAAVLRPLQQEDAARFHQLINDWDICRRLPEAPFPYPLETAEAWV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
AAAVADRAAGRAHEFAILDADGSMIGSAGLRLDAARGSAALGYWIGRRFWRRGHAGAAVA
HHHHHHHHCCCCEEEEEEECCCCEECCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHH
ELLHWGFAELPIATIEATVAADNAASIALLERAGFSATGRGHAKFIGQPGASLPVMRYAI
HHHHCCHHHCCHHHHEEEEECCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHH
TREALALRHLAAPEPVAAPAAAKPLVLVAACALVDIEGRILLARRPPGKKMAGLWEFPGG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEECCCEEEEEECCCCCCCCCEEECCCC
KLAPGETPERALVREMEEELGILLREEDVAPFAFASHAYDQFHLLMPLYLARRWSGTPEP
CCCCCCCHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
REGQALAWVPPDRLDEYPMPPADRPLLPLLRDFL
CCCCEEEECCCHHCCCCCCCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; H2O [C]

Specific reaction: CTP + H2O = CMP + diphosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 11053429 [H]