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The map label for this gene is paaF [C]

Identifier: 148260515

GI number: 148260515

Start: 1676034

End: 1676822

Strand: Reverse

Name: paaF [C]

Synonym: Acry_1515

Alternate gene names: 148260515

Gene position: 1676822-1676034 (Counterclockwise)

Preceding gene: 148260516

Following gene: 148260514

Centisome position: 49.48

GC content: 71.74

Gene sequence:

>789_bases
ATGAGCGAACCGGTTGAATTTTCCCTGGCCGAAGACGGCACCGCGACGATCTCGATGAACCGCCCCGCGCTGCACAACGC
GTTCGACGAGGGGATGATCGCGGCGCTGACCGCCGCCTGCCTGCGGGCGGGCGAGGATCCGCGCGTCCGCCGCGTGGTGC
TGGAGGGGCGGGGGAAGAGTTTTTCCGCGGGCGCCGATCTCAACTGGATGCGTCGCAGCGCGGCTTACACGGCCGAGCAG
AACCACGCGGACGCGCTGGGGCTGGGCCGGCTGATGCGGACGCTGAACGACCTGCCGAAGCCGGTGATCGGGCTGGTGCA
GGGGGCGGCCTTCGGCGGCGGCGCCGGGCTTGCGGCGTGCTGCGACATCGTGATCGCCGCGGAGGATGCGAAATTCTGCT
TCTCCGAGGTCCGGCTCGGCCTGATCCCGGCGGTGATCAGCCCCTATGTCGTCGCCGCGATCGGGCCGCACCAGGCGCGG
CGGTATTTTTGCTCCGCCGAGGTGATCCCCGCGCCGGAGGCGATGCGGATCGGACTCGCGCATATCGTGGCGCCGCGCGA
CGGGCTGGCGGCGGCGCGGGATGCGATGATCGCGACCCTCGCCCAGGGCGGGCCCGAGGCGCAGGCGGATGCGAAGGCGC
TGGTCGGCGATGTCGCGGGCCGGGATATCGATGATGCGCTGGTGAACATGACGGCGCGGCGCATCGCCGATCGCCGCGCG
AGCGCCGAGGCGCAGGAAGGCCTGGCCGCGTTTCTGGAGAAACGCGCGCCGTCCTGGCGGGCGGGGTGA

Upstream 100 bases:

>100_bases
GACCCGGCGGATACGCGGATGGTGCTGGCGCTCGGGCTCTCGGCGAGCCTGAACGCGCCGATCGAGCCGACGCGGTTCGG
CCTGTTCAGGATGTGAGGCG

Downstream 100 bases:

>100_bases
GCGCGATGTTCGGATCGGTCCTGATCGCCAATCGCGGAGAGATCGCCTGCCGGGTGATCCGCACGGCGCGGCGGATGGGG
CTGCGCAGCATCGCGGTGTT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSEPVEFSLAEDGTATISMNRPALHNAFDEGMIAALTAACLRAGEDPRVRRVVLEGRGKSFSAGADLNWMRRSAAYTAEQ
NHADALGLGRLMRTLNDLPKPVIGLVQGAAFGGGAGLAACCDIVIAAEDAKFCFSEVRLGLIPAVISPYVVAAIGPHQAR
RYFCSAEVIPAPEAMRIGLAHIVAPRDGLAAARDAMIATLAQGGPEAQADAKALVGDVAGRDIDDALVNMTARRIADRRA
SAEAQEGLAAFLEKRAPSWRAG

Sequences:

>Translated_262_residues
MSEPVEFSLAEDGTATISMNRPALHNAFDEGMIAALTAACLRAGEDPRVRRVVLEGRGKSFSAGADLNWMRRSAAYTAEQ
NHADALGLGRLMRTLNDLPKPVIGLVQGAAFGGGAGLAACCDIVIAAEDAKFCFSEVRLGLIPAVISPYVVAAIGPHQAR
RYFCSAEVIPAPEAMRIGLAHIVAPRDGLAAARDAMIATLAQGGPEAQADAKALVGDVAGRDIDDALVNMTARRIADRRA
SAEAQEGLAAFLEKRAPSWRAG
>Mature_261_residues
SEPVEFSLAEDGTATISMNRPALHNAFDEGMIAALTAACLRAGEDPRVRRVVLEGRGKSFSAGADLNWMRRSAAYTAEQN
HADALGLGRLMRTLNDLPKPVIGLVQGAAFGGGAGLAACCDIVIAAEDAKFCFSEVRLGLIPAVISPYVVAAIGPHQARR
YFCSAEVIPAPEAMRIGLAHIVAPRDGLAAARDAMIATLAQGGPEAQADAKALVGDVAGRDIDDALVNMTARRIADRRAS
AEAQEGLAAFLEKRAPSWRAG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=268, Percent_Identity=29.1044776119403, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI31542718, Length=263, Percent_Identity=27.3764258555133, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI157694516, Length=231, Percent_Identity=25.1082251082251, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI213417737, Length=232, Percent_Identity=25.4310344827586, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI70995211, Length=151, Percent_Identity=31.7880794701987, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=32.0158102766798, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=28.2442748091603, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787660, Length=266, Percent_Identity=34.2105263157895, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI221142681, Length=271, Percent_Identity=29.1512915129151, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082183, Length=267, Percent_Identity=26.9662921348315, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=259, Percent_Identity=30.1158301158301, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=255, Percent_Identity=28.2352941176471, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=238, Percent_Identity=26.890756302521, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=178, Percent_Identity=30.8988764044944, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=246, Percent_Identity=26.8292682926829, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=231, Percent_Identity=26.8398268398268, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=29.2181069958848, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=29.2181069958848, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24653139, Length=259, Percent_Identity=31.6602316602317, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=23.3716475095785, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19920382, Length=208, Percent_Identity=28.3653846153846, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27432; Mature: 27300

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPVEFSLAEDGTATISMNRPALHNAFDEGMIAALTAACLRAGEDPRVRRVVLEGRGKS
CCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCC
FSAGADLNWMRRSAAYTAEQNHADALGLGRLMRTLNDLPKPVIGLVQGAAFGGGAGLAAC
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHH
CDIVIAAEDAKFCFSEVRLGLIPAVISPYVVAAIGPHQARRYFCSAEVIPAPEAMRIGLA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHCCCCCCCCHHHHHHHH
HIVAPRDGLAAARDAMIATLAQGGPEAQADAKALVGDVAGRDIDDALVNMTARRIADRRA
HHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SAEAQEGLAAFLEKRAPSWRAG
HHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEPVEFSLAEDGTATISMNRPALHNAFDEGMIAALTAACLRAGEDPRVRRVVLEGRGKS
CCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCC
FSAGADLNWMRRSAAYTAEQNHADALGLGRLMRTLNDLPKPVIGLVQGAAFGGGAGLAAC
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHH
CDIVIAAEDAKFCFSEVRLGLIPAVISPYVVAAIGPHQARRYFCSAEVIPAPEAMRIGLA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHCCCCCCCCHHHHHHHH
HIVAPRDGLAAARDAMIATLAQGGPEAQADAKALVGDVAGRDIDDALVNMTARRIADRRA
HHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SAEAQEGLAAFLEKRAPSWRAG
HHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA