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The map label for this gene is tetA [H]

Identifier: 148260445

GI number: 148260445

Start: 1605082

End: 1606323

Strand: Reverse

Name: tetA [H]

Synonym: Acry_1444

Alternate gene names: 148260445

Gene position: 1606323-1605082 (Counterclockwise)

Preceding gene: 148260446

Following gene: 148260442

Centisome position: 47.39

GC content: 71.66

Gene sequence:

>1242_bases
ATGAGCGGTTCCACGTCCCGCAGGCGCGGGGCCGCTTTCGGCTTCATCATGGCCACGATCCTGCTGGACATGCTGGCCAT
GGCGATCGTGCTGCCGGTCCTGCCCGCGCTCATCACCGGGCTGGTCGGCGGGGGCGCCGGGCTGAGCGCGCATGTGTTCG
GCGCCTTCGGTGCCGCCTGGGCGGCGATGCAGTTCATTGCCTCGCCGATGCTTGGCGCGCTGTCCGACCGGTTCGGCCGG
CGGCCGGTGATCCTGCTCTCCTGCCTCGGCCAGGCGGTGGATTTCACCGTGATGGCGCTGGCTCCATCGGTCGGCTGGCT
GCTCGCCGGGCGGATTCTCTCCGGCGCTTCCTCGGCCAACATCGCCGCCGCGAATGCATATATTTCCGATGTCACGCCGC
CCGAGCGGCGGGCGGCGGCGTTCGGCGTCTCGGGCGTTGCCGCGGCGATCGGCTTTGTGCTCGGACCGGCGTTGGGCGGT
CTGCTCGGCGCGGTTTCGCTGCGCCTGCCGTTCTGGTTCGCCGCAGGTCTTTCCCTCGCCAACTTCCTCTACGGTCTGGT
GGCGCTGCCGGAATCGCTCGCGCCGCGCCGCCGCCGGCGGATCGGCTGGCGGGGCCTCAACCCGCTGGATGCGCTGCGCT
TCTATCGCCGGCCGGGCGTGGCCGCGCCCGCGACGATCTATGCGCTCGGCACGCTCGCGCTGCAGGCGGTGCCTTCGGTC
ATGGTGCTTTACGTGCAGGAGCGGTTCGGCTGGCGCCCACAGCTGATCGGCGCCACCCTCGCCGCCGCCGGGGTCGGGTT
TGCCGTGGTCGGCGGCGTTGTGGTCGGGCCGATGGTGCGCCGGCTTGGCGAGCCGAGAACGCTTATCCTTGGCCTGATCG
GCGGCGCCGCGGGGTTTGTGCTGTTCGGTCTCGCGCCCGATTCGCGGATTTTCTGGATCGGCGTACCGGTTCTCTCGGCC
TGGGGGCTGGCGATGCCTGCGGTGCAGGGGATCATGGCGCACCGGATTGGCGGGCGCGAGCAGGGGCGGCTGCAGGGGTT
GCTCGCCAGCCTGAACGGGCTGACCGCGCTGGTCGGCCCGTTGCTGTTCACCACGATCTACGCGGCGACGCTGGGGCCTG
ACGGCACGGCGGACGTGCCGCGCTCGGGCACCGCCCTCGTCGTCGCCGGCCTGCTGCTGCTCGCCTCGCTGATTCCGCTG
CGCTGGGCGGTGCGGTATCAGGCCGGCAGGCCGAACCCGTAG

Upstream 100 bases:

>100_bases
TATCGCCGGTGTTCCACGCCATCAAGTCGGACCGCACGCGCGACGTGACCACCAGCGCCGACTACGCCTGAGGGATGGTG
CGTCTTTCGCGCCAGTGTCG

Downstream 100 bases:

>100_bases
GCGTCCATCCGCAGGATGGCGTGCTCGGTCGAGTTGTGCAGCCGCCGTGCCGCGCTGCCCTCTCCGACGCCGATCGCGGT
GAAGAGCGGCAGCAGATGCT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: TetA(A) [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSGSTSRRRGAAFGFIMATILLDMLAMAIVLPVLPALITGLVGGGAGLSAHVFGAFGAAWAAMQFIASPMLGALSDRFGR
RPVILLSCLGQAVDFTVMALAPSVGWLLAGRILSGASSANIAAANAYISDVTPPERRAAAFGVSGVAAAIGFVLGPALGG
LLGAVSLRLPFWFAAGLSLANFLYGLVALPESLAPRRRRRIGWRGLNPLDALRFYRRPGVAAPATIYALGTLALQAVPSV
MVLYVQERFGWRPQLIGATLAAAGVGFAVVGGVVVGPMVRRLGEPRTLILGLIGGAAGFVLFGLAPDSRIFWIGVPVLSA
WGLAMPAVQGIMAHRIGGREQGRLQGLLASLNGLTALVGPLLFTTIYAATLGPDGTADVPRSGTALVVAGLLLLASLIPL
RWAVRYQAGRPNP

Sequences:

>Translated_413_residues
MSGSTSRRRGAAFGFIMATILLDMLAMAIVLPVLPALITGLVGGGAGLSAHVFGAFGAAWAAMQFIASPMLGALSDRFGR
RPVILLSCLGQAVDFTVMALAPSVGWLLAGRILSGASSANIAAANAYISDVTPPERRAAAFGVSGVAAAIGFVLGPALGG
LLGAVSLRLPFWFAAGLSLANFLYGLVALPESLAPRRRRRIGWRGLNPLDALRFYRRPGVAAPATIYALGTLALQAVPSV
MVLYVQERFGWRPQLIGATLAAAGVGFAVVGGVVVGPMVRRLGEPRTLILGLIGGAAGFVLFGLAPDSRIFWIGVPVLSA
WGLAMPAVQGIMAHRIGGREQGRLQGLLASLNGLTALVGPLLFTTIYAATLGPDGTADVPRSGTALVVAGLLLLASLIPL
RWAVRYQAGRPNP
>Mature_412_residues
SGSTSRRRGAAFGFIMATILLDMLAMAIVLPVLPALITGLVGGGAGLSAHVFGAFGAAWAAMQFIASPMLGALSDRFGRR
PVILLSCLGQAVDFTVMALAPSVGWLLAGRILSGASSANIAAANAYISDVTPPERRAAAFGVSGVAAAIGFVLGPALGGL
LGAVSLRLPFWFAAGLSLANFLYGLVALPESLAPRRRRRIGWRGLNPLDALRFYRRPGVAAPATIYALGTLALQAVPSVM
VLYVQERFGWRPQLIGATLAAAGVGFAVVGGVVVGPMVRRLGEPRTLILGLIGGAAGFVLFGLAPDSRIFWIGVPVLSAW
GLAMPAVQGIMAHRIGGREQGRLQGLLASLNGLTALVGPLLFTTIYAATLGPDGTADVPRSGTALVVAGLLLLASLIPLR
WAVRYQAGRPNP

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI39753965, Length=374, Percent_Identity=24.5989304812834, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI94721311, Length=369, Percent_Identity=26.0162601626016, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI225703104, Length=200, Percent_Identity=34.5, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI225703102, Length=200, Percent_Identity=34.5, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI217416366, Length=389, Percent_Identity=25.4498714652956, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787291, Length=360, Percent_Identity=25.5555555555556, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71996331, Length=353, Percent_Identity=24.929178470255, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17551570, Length=224, Percent_Identity=29.0178571428571, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357569, Length=337, Percent_Identity=25.8160237388724, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161080926, Length=337, Percent_Identity=25.8160237388724, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161080924, Length=337, Percent_Identity=25.8160237388724, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24656463, Length=337, Percent_Identity=25.8160237388724, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24656468, Length=337, Percent_Identity=25.8160237388724, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17738085, Length=338, Percent_Identity=25.7396449704142, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI221513297, Length=312, Percent_Identity=25.3205128205128, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24652011, Length=335, Percent_Identity=25.3731343283582, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42543; Mature: 42412

Theoretical pI: Translated: 12.16; Mature: 12.16

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SGSTSRRRGAAFGFIMATILLDMLAMAIVLPVLPALITGLVGGGAGLSAHVFGAFGAAW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AAMQFIASPMLGALSDRFGRRPVILLSCLGQAVDFTVMALAPSVGWLLAGRILSGASSAN
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IAAANAYISDVTPPERRAAAFGVSGVAAAIGFVLGPALGGLLGAVSLRLPFWFAAGLSLA
EEEHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFLYGLVALPESLAPRRRRRIGWRGLNPLDALRFYRRPGVAAPATIYALGTLALQAVPSV
HHHHHHHHCCHHHCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
MVLYVQERFGWRPQLIGATLAAAGVGFAVVGGVVVGPMVRRLGEPRTLILGLIGGAAGFV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
LFGLAPDSRIFWIGVPVLSAWGLAMPAVQGIMAHRIGGREQGRLQGLLASLNGLTALVGP
EEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LLFTTIYAATLGPDGTADVPRSGTALVVAGLLLLASLIPLRWAVRYQAGRPNP
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312499; 6310527 [H]