Click here to switch to the map view.

The map label for this gene is recJ [H]

Identifier: 148260299

GI number: 148260299

Start: 1448420

End: 1450204

Strand: Reverse

Name: recJ [H]

Synonym: Acry_1296

Alternate gene names: 148260299

Gene position: 1450204-1448420 (Counterclockwise)

Preceding gene: 148260300

Following gene: 148260297

Centisome position: 42.79

GC content: 73.28

Gene sequence:

>1785_bases
GTGGACGCCGGCCTGAACGGGGCGGCGCCGGCTTTCGGGGTCGAACGCAGCCTGACCGGCCGGCGCTGGCTCTGGCGCCC
GGCCGCGGACCGCACGGCGCAGGCCATCGCCCAGCGCTGCGGCGTGCCGGAGGTGATCGGCCGGCTGCTCGCCGGGCGCG
GCGTGATGCCGGATCATGCCGCGGATTTCCTCGAGCCGACGCTGCGCGCCTATCTGCCCGATCCCTCGGCGCTGACCGAC
ATGGATGCCGCGGCGGCGCGGATCGCCGATTCAGTGCAGCGCGGCGCGACCATCGCGATCTTTGGCGATTACGACGTCGA
CGGCGCCTGTTCGGGCGCGCTCATGGCGCTGGGCCTTGGGGAACTTGGCGCGACGACCATCAATTACGTGCCGGATCGGC
TCCGCGAGGGCTACGGGCCGAACGCGCCGGCGCTGCGGGCGCTTGCCGGGCAGGGGGCGGAACTGATCGTCTGCGTCGAT
TGCGGAACGGCGGCGCATGAGGCGCTCGCGGCGCTCGACGGCCGGGCGGATGCCATCGTTCTCGACCATCACAAGAGCGA
GGGGGCTCCGCCCGCCATCGTGGCGACGGTGAACCCGAACCGCGCTGACGATGCGTCGGGCCTCGGCAACGTTTGCGCGG
CGACGATCAGCTTCCTCACCCTCGTCGCCGTGCAGCGCGAACTTCGCCGGCGGGGCTTCTTCGCGACGCGGCGCGAGATC
GACCTGCTGTCGATGCTCGACCTCGTCGCGCTCGCGACGGTCTGCGACGTGATGCCGCTCACCGGCCTTAACCGCGCGCT
GGTGACCCAGGGGCTGAAGGTGATGGCGCGGCGGGAACGGCCGGGAATCGCGGCATTGCTCGACGTCGCGCAGGTGAGGG
AGGCGCCGGGCACGATGACCTGCGGCTTCGCGATCGGCCCGCGGATCAACGCCGCCGGCCGCATCGCCGAGGCGGATCTC
GGGCTGCGCACGCTGCTCGCCGCCGAGCCGGCGACGGCGATGGTGCTGGCGCGCCGCCTCGACGAGATCAACCGCGAGCG
CCAGGCGGTGGAGGCCGGGCTGCTGGACGAGGCGATGACCGACGCCGAGGCCCAGTTCGCCGCCGGCGCGCCGGTCGCGC
TGGTTGCCGGCGAGCAATGGCATCCGGGCGTGGTCGGCATTGTCGCCGGCCGGATCAAGGAAACCTTCAACCGTCCGGCC
CTGGTCGCGGGAATCGCGGAGGGCAGGGCGGTGGGCTCTGGCCGCTCGGTGCCGGGGGTCGATCTCGGCAGCGCGATCAT
CGCCGCGCGCGAGGCGGGGCTGCTGATCAAGGGCGGCGGCCACGCCATGGCCGCGGGTTTCACGGTGGAAGCCGGGCGGA
TCGAGGCGCTGCGCGGCTTCCTCGCAGAGCGGTTGGCGGTGGCCGCGACGCTGCCGGACGCGGCCGATCTGCCGATCGAG
GGCATGGCGGCGGTCGGCGGGATCGACGAGGCGCTCGCCGCGGCGATCGGCCGCGTCGGGCCGTTCGGCGCCGGCAACGA
GGAGCCGGTGTTCGTGGTGCCGCGGGCGCGGGTCCTGCGGGCGGACCGCATCGGCCGGAACGAGGCGACGATCCGCGCCT
TCGTCGAGGGCGAGGCCGGTGGCCGGCTGAAGACGATCCTGTTCCGCGCCGGAGACGGCGACCTTGCGGCCGCGCTGGCC
CGTCCGGGCGGCGCACCGCTCAATCTTGCAGGTCACATCCGCGCGGAACAATGGAATGGCAGAACCTCGGTCAGCCTGAT
CCTGATCGACGCCGCTCCCGCTTGA

Upstream 100 bases:

>100_bases
GGCGGTATGCGACCGGCGCGGTGACCCACTCGATCGTGATGCGGTCGAAATCCGGCACCGTCCGCTATATCGAGGCGCAT
CACAACTTCCAGACCAAGCC

Downstream 100 bases:

>100_bases
CCTGCCCCCACCACTCGGCTACCCACGCTTTCAGAAAGTCCCGTTCGTCTAGAGGCCTAGGACGTCGCCCTCTCACGGCG
ATAACAGGGGTTCGAATCCC

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MDAGLNGAAPAFGVERSLTGRRWLWRPAADRTAQAIAQRCGVPEVIGRLLAGRGVMPDHAADFLEPTLRAYLPDPSALTD
MDAAAARIADSVQRGATIAIFGDYDVDGACSGALMALGLGELGATTINYVPDRLREGYGPNAPALRALAGQGAELIVCVD
CGTAAHEALAALDGRADAIVLDHHKSEGAPPAIVATVNPNRADDASGLGNVCAATISFLTLVAVQRELRRRGFFATRREI
DLLSMLDLVALATVCDVMPLTGLNRALVTQGLKVMARRERPGIAALLDVAQVREAPGTMTCGFAIGPRINAAGRIAEADL
GLRTLLAAEPATAMVLARRLDEINRERQAVEAGLLDEAMTDAEAQFAAGAPVALVAGEQWHPGVVGIVAGRIKETFNRPA
LVAGIAEGRAVGSGRSVPGVDLGSAIIAAREAGLLIKGGGHAMAAGFTVEAGRIEALRGFLAERLAVAATLPDAADLPIE
GMAAVGGIDEALAAAIGRVGPFGAGNEEPVFVVPRARVLRADRIGRNEATIRAFVEGEAGGRLKTILFRAGDGDLAAALA
RPGGAPLNLAGHIRAEQWNGRTSVSLILIDAAPA

Sequences:

>Translated_594_residues
MDAGLNGAAPAFGVERSLTGRRWLWRPAADRTAQAIAQRCGVPEVIGRLLAGRGVMPDHAADFLEPTLRAYLPDPSALTD
MDAAAARIADSVQRGATIAIFGDYDVDGACSGALMALGLGELGATTINYVPDRLREGYGPNAPALRALAGQGAELIVCVD
CGTAAHEALAALDGRADAIVLDHHKSEGAPPAIVATVNPNRADDASGLGNVCAATISFLTLVAVQRELRRRGFFATRREI
DLLSMLDLVALATVCDVMPLTGLNRALVTQGLKVMARRERPGIAALLDVAQVREAPGTMTCGFAIGPRINAAGRIAEADL
GLRTLLAAEPATAMVLARRLDEINRERQAVEAGLLDEAMTDAEAQFAAGAPVALVAGEQWHPGVVGIVAGRIKETFNRPA
LVAGIAEGRAVGSGRSVPGVDLGSAIIAAREAGLLIKGGGHAMAAGFTVEAGRIEALRGFLAERLAVAATLPDAADLPIE
GMAAVGGIDEALAAAIGRVGPFGAGNEEPVFVVPRARVLRADRIGRNEATIRAFVEGEAGGRLKTILFRAGDGDLAAALA
RPGGAPLNLAGHIRAEQWNGRTSVSLILIDAAPA
>Mature_594_residues
MDAGLNGAAPAFGVERSLTGRRWLWRPAADRTAQAIAQRCGVPEVIGRLLAGRGVMPDHAADFLEPTLRAYLPDPSALTD
MDAAAARIADSVQRGATIAIFGDYDVDGACSGALMALGLGELGATTINYVPDRLREGYGPNAPALRALAGQGAELIVCVD
CGTAAHEALAALDGRADAIVLDHHKSEGAPPAIVATVNPNRADDASGLGNVCAATISFLTLVAVQRELRRRGFFATRREI
DLLSMLDLVALATVCDVMPLTGLNRALVTQGLKVMARRERPGIAALLDVAQVREAPGTMTCGFAIGPRINAAGRIAEADL
GLRTLLAAEPATAMVLARRLDEINRERQAVEAGLLDEAMTDAEAQFAAGAPVALVAGEQWHPGVVGIVAGRIKETFNRPA
LVAGIAEGRAVGSGRSVPGVDLGSAIIAAREAGLLIKGGGHAMAAGFTVEAGRIEALRGFLAERLAVAATLPDAADLPIE
GMAAVGGIDEALAAAIGRVGPFGAGNEEPVFVVPRARVLRADRIGRNEATIRAFVEGEAGGRLKTILFRAGDGDLAAALA
RPGGAPLNLAGHIRAEQWNGRTSVSLILIDAAPA

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=586, Percent_Identity=33.4470989761092, Blast_Score=234, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 61205; Mature: 61205

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAGLNGAAPAFGVERSLTGRRWLWRPAADRTAQAIAQRCGVPEVIGRLLAGRGVMPDHA
CCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
ADFLEPTLRAYLPDPSALTDMDAAAARIADSVQRGATIAIFGDYDVDGACSGALMALGLG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCC
ELGATTINYVPDRLREGYGPNAPALRALAGQGAELIVCVDCGTAAHEALAALDGRADAIV
HHCCHHHHHCHHHHHCCCCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCCEEE
LDHHKSEGAPPAIVATVNPNRADDASGLGNVCAATISFLTLVAVQRELRRRGFFATRREI
EECCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
DLLSMLDLVALATVCDVMPLTGLNRALVTQGLKVMARRERPGIAALLDVAQVREAPGTMT
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEE
CGFAIGPRINAAGRIAEADLGLRTLLAAEPATAMVLARRLDEINRERQAVEAGLLDEAMT
ECEEECCCCCCCCCEEHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAEAQFAAGAPVALVAGEQWHPGVVGIVAGRIKETFNRPALVAGIAEGRAVGSGRSVPGV
CCHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
DLGSAIIAAREAGLLIKGGGHAMAAGFTVEAGRIEALRGFLAERLAVAATLPDAADLPIE
CHHHHHHHHHHCCEEEECCCCEEEECEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GMAAVGGIDEALAAAIGRVGPFGAGNEEPVFVVPRARVLRADRIGRNEATIRAFVEGEAG
CHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCEEEEEEECCCC
GRLKTILFRAGDGDLAAALARPGGAPLNLAGHIRAEQWNGRTSVSLILIDAAPA
CEEEEEEEECCCCHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEEEEEEECCCC
>Mature Secondary Structure
MDAGLNGAAPAFGVERSLTGRRWLWRPAADRTAQAIAQRCGVPEVIGRLLAGRGVMPDHA
CCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
ADFLEPTLRAYLPDPSALTDMDAAAARIADSVQRGATIAIFGDYDVDGACSGALMALGLG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCC
ELGATTINYVPDRLREGYGPNAPALRALAGQGAELIVCVDCGTAAHEALAALDGRADAIV
HHCCHHHHHCHHHHHCCCCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCCEEE
LDHHKSEGAPPAIVATVNPNRADDASGLGNVCAATISFLTLVAVQRELRRRGFFATRREI
EECCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
DLLSMLDLVALATVCDVMPLTGLNRALVTQGLKVMARRERPGIAALLDVAQVREAPGTMT
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEE
CGFAIGPRINAAGRIAEADLGLRTLLAAEPATAMVLARRLDEINRERQAVEAGLLDEAMT
ECEEECCCCCCCCCEEHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAEAQFAAGAPVALVAGEQWHPGVVGIVAGRIKETFNRPALVAGIAEGRAVGSGRSVPGV
CCHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
DLGSAIIAAREAGLLIKGGGHAMAAGFTVEAGRIEALRGFLAERLAVAATLPDAADLPIE
CHHHHHHHHHHCCEEEECCCCEEEECEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GMAAVGGIDEALAAAIGRVGPFGAGNEEPVFVVPRARVLRADRIGRNEATIRAFVEGEAG
CHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCEEEEEEECCCC
GRLKTILFRAGDGDLAAALARPGGAPLNLAGHIRAEQWNGRTSVSLILIDAAPA
CEEEEEEEECCCCHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]