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The map label for this gene is mii [H]

Identifier: 148260269

GI number: 148260269

Start: 1412161

End: 1413315

Strand: Reverse

Name: mii [H]

Synonym: Acry_1266

Alternate gene names: 148260269

Gene position: 1413315-1412161 (Counterclockwise)

Preceding gene: 148260270

Following gene: 148260266

Centisome position: 41.7

GC content: 73.33

Gene sequence:

>1155_bases
GTGGCGCTCCGGGCAATCCGCGCCGTGTTCATGCGCGGCGGCACCTCGAAGGCGCTGATCTTCCGCGCCGGCGATCTCCC
CGCCGATCGGGCGGACTGGGCGCCGATCTTCCTCGCCGCGATGGGCTCGCCGGACCCGGCGATGCGCCAGCTCGACGGCA
TGGGCGGCGGCGTGTCCTCGCTGTCGAAATGCTGCGTCGTCGGCCCGCCGAGCCGGCCGGATGCGGATGTCGACTACACG
TTCGCGCAGGTCGCGATCGACCGGCCGGAGGTCGATTACGCCAGTAATTGCGGCAACATGTCCTCGGCGATCGGCCCCTT
CGCGGTGGATGAGGGGCTGGTCGCCCCGCCTGCGGGGAACGAGGCGGTGGTGCGCATCCACAACACCAATACCGGCAAGA
TCATCGTCGCCCGGTTCCCGATGGAAGATGGCCGCGCCGCGGTGACGGGTGACCTCGCGATCGACGGCGTCGCCGGCACC
GGGGCACCGATCCGCCTCGATTTCACCGATCCCGGCGGGGCCGGCACCGGGCGCCTGCTGCCGACCGGCAACCTGACGGA
CCGGCTCGACGTACCCGGCCTCGGGCCGCTGCGCGTCACCCTGATCGACGCCGCCAATCCCTGCGTGCTGGTCGCCGCCG
CCGATCTCGGGATGACCGCGACCGAAACGCCGGCGGCGATGGAGCGCGACACCGCGCTGATCGCCGGGATCGAGGCGCTG
CGGCGCGCGGCTTCGGTGCGGATGGGCCTGGCCGCCGATCTCGAGGCGGCGGCGCGGATCATCAGCGTGCCGAAAGTGGG
CATTCTCGCGCCGCCGGCGGATGCGCCCGTCCTGTCAGGGGCGGTGCAGACGGCCGCGAGCGTCGACATTCTGGCGCGCA
TGATGTCGTCCGGCCAGCCGCATCGCGCGGTGCCGCTGACCGGCGCGCTCTGCCTCGCCGTGGCCTGCCGGCTGCCCGGC
ACCGTGGCGCATGACCTGGTCCGGCCGGGCGAGGCGACCGATCCGGTGCGGGTCGGCCATCCTTCCGGCGCGATCCTCGT
CGCCGCCGGCGTGTCCTCAGGGCCGGACGGACCCCGCGTCTCGCACGCCACCGTGTTCCGGACCGCGCGGCGGCTGTTCC
AGGGCGAGGTGCTCCATGTCGGCCCCCGCGTATAG

Upstream 100 bases:

>100_bases
GCGATGCGCGCGCATGTCGAGAACGGCTATCGCCGGCTGACCGGCCAGGCGCTGGTCCCGGGTCCGGCATCCGCCGCGCC
GGCGGCGTCGGGCTGATCCG

Downstream 100 bases:

>100_bases
GGGTTACGCTACCGGCTTGTCGGGGAAGGCGAGTTCGTGGCGCGAGCGCCAGTCCGGTGTCACGTAGTTCACGATCAGCG
AGCGCCGCACCCCGCTGATC

Product: hypothetical protein

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MALRAIRAVFMRGGTSKALIFRAGDLPADRADWAPIFLAAMGSPDPAMRQLDGMGGGVSSLSKCCVVGPPSRPDADVDYT
FAQVAIDRPEVDYASNCGNMSSAIGPFAVDEGLVAPPAGNEAVVRIHNTNTGKIIVARFPMEDGRAAVTGDLAIDGVAGT
GAPIRLDFTDPGGAGTGRLLPTGNLTDRLDVPGLGPLRVTLIDAANPCVLVAAADLGMTATETPAAMERDTALIAGIEAL
RRAASVRMGLAADLEAAARIISVPKVGILAPPADAPVLSGAVQTAASVDILARMMSSGQPHRAVPLTGALCLAVACRLPG
TVAHDLVRPGEATDPVRVGHPSGAILVAAGVSSGPDGPRVSHATVFRTARRLFQGEVLHVGPRV

Sequences:

>Translated_384_residues
MALRAIRAVFMRGGTSKALIFRAGDLPADRADWAPIFLAAMGSPDPAMRQLDGMGGGVSSLSKCCVVGPPSRPDADVDYT
FAQVAIDRPEVDYASNCGNMSSAIGPFAVDEGLVAPPAGNEAVVRIHNTNTGKIIVARFPMEDGRAAVTGDLAIDGVAGT
GAPIRLDFTDPGGAGTGRLLPTGNLTDRLDVPGLGPLRVTLIDAANPCVLVAAADLGMTATETPAAMERDTALIAGIEAL
RRAASVRMGLAADLEAAARIISVPKVGILAPPADAPVLSGAVQTAASVDILARMMSSGQPHRAVPLTGALCLAVACRLPG
TVAHDLVRPGEATDPVRVGHPSGAILVAAGVSSGPDGPRVSHATVFRTARRLFQGEVLHVGPRV
>Mature_383_residues
ALRAIRAVFMRGGTSKALIFRAGDLPADRADWAPIFLAAMGSPDPAMRQLDGMGGGVSSLSKCCVVGPPSRPDADVDYTF
AQVAIDRPEVDYASNCGNMSSAIGPFAVDEGLVAPPAGNEAVVRIHNTNTGKIIVARFPMEDGRAAVTGDLAIDGVAGTG
APIRLDFTDPGGAGTGRLLPTGNLTDRLDVPGLGPLRVTLIDAANPCVLVAAADLGMTATETPAAMERDTALIAGIEALR
RAASVRMGLAADLEAAARIISVPKVGILAPPADAPVLSGAVQTAASVDILARMMSSGQPHRAVPLTGALCLAVACRLPGT
VAHDLVRPGEATDPVRVGHPSGAILVAAGVSSGPDGPRVSHATVFRTARRLFQGEVLHVGPRV

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=377, Percent_Identity=37.9310344827586, Blast_Score=207, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 39106; Mature: 38975

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRAIRAVFMRGGTSKALIFRAGDLPADRADWAPIFLAAMGSPDPAMRQLDGMGGGVSS
CHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCHHH
LSKCCVVGPPSRPDADVDYTFAQVAIDRPEVDYASNCGNMSSAIGPFAVDEGLVAPPAGN
HCCEEEECCCCCCCCCCCEEEEEEEECCCCCCHHHCCCCCHHCCCCEECCCCEECCCCCC
EAVVRIHNTNTGKIIVARFPMEDGRAAVTGDLAIDGVAGTGAPIRLDFTDPGGAGTGRLL
CEEEEEEECCCCEEEEEECCCCCCCEEEEECEEEECCCCCCCEEEEEECCCCCCCCCCEE
PTGNLTDRLDVPGLGPLRVTLIDAANPCVLVAAADLGMTATETPAAMERDTALIAGIEAL
ECCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
RRAASVRMGLAADLEAAARIISVPKVGILAPPADAPVLSGAVQTAASVDILARMMSSGQP
HHHHHHHHCHHHCHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
HRAVPLTGALCLAVACRLPGTVAHDLVRPGEATDPVRVGHPSGAILVAAGVSSGPDGPRV
CCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEECCCCCCEEEEECCCCCCCCCCC
SHATVFRTARRLFQGEVLHVGPRV
CHHHHHHHHHHHHCCCEEECCCCC
>Mature Secondary Structure 
ALRAIRAVFMRGGTSKALIFRAGDLPADRADWAPIFLAAMGSPDPAMRQLDGMGGGVSS
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCHHH
LSKCCVVGPPSRPDADVDYTFAQVAIDRPEVDYASNCGNMSSAIGPFAVDEGLVAPPAGN
HCCEEEECCCCCCCCCCCEEEEEEEECCCCCCHHHCCCCCHHCCCCEECCCCEECCCCCC
EAVVRIHNTNTGKIIVARFPMEDGRAAVTGDLAIDGVAGTGAPIRLDFTDPGGAGTGRLL
CEEEEEEECCCCEEEEEECCCCCCCEEEEECEEEECCCCCCCEEEEEECCCCCCCCCCEE
PTGNLTDRLDVPGLGPLRVTLIDAANPCVLVAAADLGMTATETPAAMERDTALIAGIEAL
ECCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
RRAASVRMGLAADLEAAARIISVPKVGILAPPADAPVLSGAVQTAASVDILARMMSSGQP
HHHHHHHHCHHHCHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
HRAVPLTGALCLAVACRLPGTVAHDLVRPGEATDPVRVGHPSGAILVAAGVSSGPDGPRV
CCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEECCCCCCEEEEECCCCCCCCCCC
SHATVFRTARRLFQGEVLHVGPRV
CHHHHHHHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA