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The map label for this gene is fadI [H]

Identifier: 148260260

GI number: 148260260

Start: 1400297

End: 1401583

Strand: Reverse

Name: fadI [H]

Synonym: Acry_1257

Alternate gene names: 148260260

Gene position: 1401583-1400297 (Counterclockwise)

Preceding gene: 148260261

Following gene: 148260259

Centisome position: 41.35

GC content: 71.17

Gene sequence:

>1287_bases
ATGCTCACCGCCCCCTCGCTCCGCCGCGTCGCGATCATCGGCGGCACGCGCATTCCCTTCGCCCGCGCCAACACCGCCTA
TGCCGAGGCCTCCAACCAGGACATGCTGACCGCCGCCCTGCAGGGCCTGGTCGACCGCTACCGCCTCGGCGGCGAACGGC
TCGGCGAGGTCGTCGCCGGCGCCGTGCTCAAGCACAGCCGCGACTTCAACCTCACCCGCGAGGCGGTGCTCTCCACCACG
CTCGGCCACGACACCCCGGCCTATGACATCCAGCAGGCCTGCGGCACCGGGCTCGAAGCGGCCCTGCTGGTCGCCAGCAA
GATCGCCCTCGGCCAGATCGACGCCGGCATCGCCGGCGGGGTCGACACCACGTCCGACGCGCCGATCGCGCTCAACGAAA
AGCTGCGCAAGCTGCTGCTCAAGGCCAATCGCGGCCGCACCACCGCCGAGAAGGTGAAAACCCTCGCCGGCATCCGCCCG
TCCATGCTGCTCCGCCCGGCGCTGCCGCGCAACGCGGAGCCGCGCACCGGCCTGTCGATGGGCGAGCATTGCGAGCAGAT
GGCGAAACGCTGGCGCATCCCCCGCGCCGCCCAGGACGAGCTCACCGTCGCCAGTCATCGCAACCTCGCCGCCGCCTACG
GACGCGGCTTCTTCAACGACCTCATCGTGCCGTTTCGCGGCCTGACGAAGGACGGCAATCTCCGCCCCGACATCGACCCG
GCGAAGCTCGCGGCGATGCCGCCGGTGTTCGACCGCAAGGGCGGCACCATGACCGCCGCCAACTCCACCCCGCTGACCGA
TGGCGCGGCCGTCGTCCTGCTCGCCTCCGAGGAATGGGCGCAGGCGCGCAACCTTCCGGTTCTCGCCTATCTGCGCACCG
GCGAGGCGGCGGCGGTCGATTTCGTCGATGGCGAGGAAGGCCTGCTGATGGCGCCGGCCTATGCCGTGCCGCGGATGCTC
GGCAAGCTCGGCATCACCTTGCAGGATTTCGATTTCTACGAAATCCACGAGGCCTTCGCCGCCCAGGTGCTCTCGACCCT
GGCCGCCTGGGAAAGCCCCGACTACGCGCGCGACCGCCTCGGGCTCGACGCCCCGCCCGGCGCCATCGACCGCGCGCGGC
TCAACGTCAACGGCAGCTCGCTCGCCACCGGCCACCCCTTCGCCGCGACCGGCGGGCGGATTCTGGCGACGCTGGCGAAG
ATGCTGGCCGAAAAGGGGGCCGGACGCGGCCTGATCTCGATCTGTGCCGCGGGCGGGCAGGGTGTCGTCGCGGTGGTAGA
GCGCTGA

Upstream 100 bases:

>100_bases
CCGGCGGCACCCGCCTGATCGAACTGCGCGACCGGCGCGAGACGAAGCCGCATCTGCGCGGCACGCTCGCCTTCCGCGCC
GCCGCCTGATGGAGACATCA

Downstream 100 bases:

>100_bases
GGGAAAGTGATATGACCGATACAATGCAGGACAGTGTCGAAACCGACGGAATCTGGTTCGCCTCCTACCGCGAAGGGGTG
CCGCACGACATTGGCGACGA

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MLTAPSLRRVAIIGGTRIPFARANTAYAEASNQDMLTAALQGLVDRYRLGGERLGEVVAGAVLKHSRDFNLTREAVLSTT
LGHDTPAYDIQQACGTGLEAALLVASKIALGQIDAGIAGGVDTTSDAPIALNEKLRKLLLKANRGRTTAEKVKTLAGIRP
SMLLRPALPRNAEPRTGLSMGEHCEQMAKRWRIPRAAQDELTVASHRNLAAAYGRGFFNDLIVPFRGLTKDGNLRPDIDP
AKLAAMPPVFDRKGGTMTAANSTPLTDGAAVVLLASEEWAQARNLPVLAYLRTGEAAAVDFVDGEEGLLMAPAYAVPRML
GKLGITLQDFDFYEIHEAFAAQVLSTLAAWESPDYARDRLGLDAPPGAIDRARLNVNGSSLATGHPFAATGGRILATLAK
MLAEKGAGRGLISICAAGGQGVVAVVER

Sequences:

>Translated_428_residues
MLTAPSLRRVAIIGGTRIPFARANTAYAEASNQDMLTAALQGLVDRYRLGGERLGEVVAGAVLKHSRDFNLTREAVLSTT
LGHDTPAYDIQQACGTGLEAALLVASKIALGQIDAGIAGGVDTTSDAPIALNEKLRKLLLKANRGRTTAEKVKTLAGIRP
SMLLRPALPRNAEPRTGLSMGEHCEQMAKRWRIPRAAQDELTVASHRNLAAAYGRGFFNDLIVPFRGLTKDGNLRPDIDP
AKLAAMPPVFDRKGGTMTAANSTPLTDGAAVVLLASEEWAQARNLPVLAYLRTGEAAAVDFVDGEEGLLMAPAYAVPRML
GKLGITLQDFDFYEIHEAFAAQVLSTLAAWESPDYARDRLGLDAPPGAIDRARLNVNGSSLATGHPFAATGGRILATLAK
MLAEKGAGRGLISICAAGGQGVVAVVER
>Mature_428_residues
MLTAPSLRRVAIIGGTRIPFARANTAYAEASNQDMLTAALQGLVDRYRLGGERLGEVVAGAVLKHSRDFNLTREAVLSTT
LGHDTPAYDIQQACGTGLEAALLVASKIALGQIDAGIAGGVDTTSDAPIALNEKLRKLLLKANRGRTTAEKVKTLAGIRP
SMLLRPALPRNAEPRTGLSMGEHCEQMAKRWRIPRAAQDELTVASHRNLAAAYGRGFFNDLIVPFRGLTKDGNLRPDIDP
AKLAAMPPVFDRKGGTMTAANSTPLTDGAAVVLLASEEWAQARNLPVLAYLRTGEAAAVDFVDGEEGLLMAPAYAVPRML
GKLGITLQDFDFYEIHEAFAAQVLSTLAAWESPDYARDRLGLDAPPGAIDRARLNVNGSSLATGHPFAATGGRILATLAK
MLAEKGAGRGLISICAAGGQGVVAVVER

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=435, Percent_Identity=35.4022988505747, Blast_Score=259, Evalue=5e-69,
Organism=Homo sapiens, GI4557237, Length=435, Percent_Identity=27.1264367816092, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4501853, Length=433, Percent_Identity=29.5612009237875, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI167614485, Length=432, Percent_Identity=27.3148148148148, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI148539872, Length=429, Percent_Identity=27.972027972028, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1788683, Length=411, Percent_Identity=38.6861313868613, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI87082165, Length=434, Percent_Identity=31.1059907834101, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788554, Length=431, Percent_Identity=30.1624129930394, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1787663, Length=434, Percent_Identity=29.7235023041475, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI48994986, Length=434, Percent_Identity=28.3410138248848, Blast_Score=135, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17551802, Length=432, Percent_Identity=33.7962962962963, Blast_Score=241, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI133906874, Length=429, Percent_Identity=26.8065268065268, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17535921, Length=434, Percent_Identity=25.5760368663594, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25147385, Length=435, Percent_Identity=25.5172413793103, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6322031, Length=431, Percent_Identity=29.9303944315545, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325229, Length=437, Percent_Identity=27.6887871853547, Blast_Score=152, Evalue=8e-38,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=36.5339578454333, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24655093, Length=427, Percent_Identity=28.5714285714286, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24640423, Length=436, Percent_Identity=26.1467889908257, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17648125, Length=391, Percent_Identity=29.156010230179, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 45233; Mature: 45233

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAPSLRRVAIIGGTRIPFARANTAYAEASNQDMLTAALQGLVDRYRLGGERLGEVVAG
CCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
AVLKHSRDFNLTREAVLSTTLGHDTPAYDIQQACGTGLEAALLVASKIALGQIDAGIAGG
HHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
VDTTSDAPIALNEKLRKLLLKANRGRTTAEKVKTLAGIRPSMLLRPALPRNAEPRTGLSM
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCH
GEHCEQMAKRWRIPRAAQDELTVASHRNLAAAYGRGFFNDLIVPFRGLTKDGNLRPDIDP
HHHHHHHHHHCCCCCCCCCCEEHHHCCCHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCH
AKLAAMPPVFDRKGGTMTAANSTPLTDGAAVVLLASEEWAQARNLPVLAYLRTGEAAAVD
HHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECHHHHHHCCCCEEEEEECCCEEEEE
FVDGEEGLLMAPAYAVPRMLGKLGITLQDFDFYEIHEAFAAQVLSTLAAWESPDYARDRL
EECCCCCEEECCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHC
GLDAPPGAIDRARLNVNGSSLATGHPFAATGGRILATLAKMLAEKGAGRGLISICAAGGQ
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEHEEECCCC
GVVAVVER
CEEEEECC
>Mature Secondary Structure
MLTAPSLRRVAIIGGTRIPFARANTAYAEASNQDMLTAALQGLVDRYRLGGERLGEVVAG
CCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
AVLKHSRDFNLTREAVLSTTLGHDTPAYDIQQACGTGLEAALLVASKIALGQIDAGIAGG
HHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
VDTTSDAPIALNEKLRKLLLKANRGRTTAEKVKTLAGIRPSMLLRPALPRNAEPRTGLSM
CCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCH
GEHCEQMAKRWRIPRAAQDELTVASHRNLAAAYGRGFFNDLIVPFRGLTKDGNLRPDIDP
HHHHHHHHHHCCCCCCCCCCEEHHHCCCHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCH
AKLAAMPPVFDRKGGTMTAANSTPLTDGAAVVLLASEEWAQARNLPVLAYLRTGEAAAVD
HHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECHHHHHHCCCCEEEEEECCCEEEEE
FVDGEEGLLMAPAYAVPRMLGKLGITLQDFDFYEIHEAFAAQVLSTLAAWESPDYARDRL
EECCCCCEEECCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHC
GLDAPPGAIDRARLNVNGSSLATGHPFAATGGRILATLAKMLAEKGAGRGLISICAAGGQ
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEHEEECCCC
GVVAVVER
CEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA