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The map label for this gene is ilvE [H]

Identifier: 148259861

GI number: 148259861

Start: 937993

End: 938892

Strand: Reverse

Name: ilvE [H]

Synonym: Acry_0850

Alternate gene names: 148259861

Gene position: 938892-937993 (Counterclockwise)

Preceding gene: 148259866

Following gene: 148259860

Centisome position: 27.7

GC content: 68.44

Gene sequence:

>900_bases
ATGAGTCTGATTCCGTTTGACGACCGCGACGGCATGATCTGGTGGGACGGCGAGCTGCGCCCCTGGCGGGACGCCAAGCT
GCACGTGCTGACGCACGGGCTGCATTACGCCTCCGCCGTCTTCGAGGGCGAGCGGTCGTATGGCGGGAACATCTTCCGCC
TGCGCGACCATACCAACCGGCTGATCCAGTCGGGCAAGCTGCTCGGCTTCGACATTCCCTTCAGCGCCGATGCGATCGAC
GCCGCCTGCAACGAGGTGCTGAAGGCGAACGGGCTGACCGACGCCTATATCCGCCCGATCGCCTGGCGCGGCTCGGAGAT
GCTCGCGGTTTCCGCCCAGCAGACCAAGATCCACCTCGCCATCGCCGCCTGGCCGTGGCCGAACCTGTTCGGCAACGACC
GGATGAAGGGCATCACCCTCGGCATGGCGCCGTGGCGCCGGCCCGATCCGCGCACCGCGCCGACCGCCTCGAAGGCCGCC
GGGCTCTACATGATCGGCACGCTCTCGAAGCACGCGGCCGAGGCGGAGGGGTTCAACGACGCGCTGATGCTCACCCTCGA
CGGGCATGTCGCCGAGGCGACGGGGGCGAACATCTTCTTCGCGATCGACGGCGAACTGCACACGCCGACGCCGGACTGCT
TCCTCGACGGCATTACCCGCCGCACGGTGATGGCGCTGGCCCGCGCGCGGCAGATTCCGGTGCATGAGCGGCACATCAAG
CCCGAGGAGATGGCGAAGGCGAGCGAGGTGTTCCTCGCCGGCACCGCCGCCGAGGTGACGCCGGTGCGCCAGATCGGCGA
GCTGACCTTCACGCCGGGGAAGATCACCGAGACGCTGATCGCGGACTATACCGCGCTCGTCCACATGTCGCCGGAAGAGG
TGGCCAAGCGGGCGGCGTGA

Upstream 100 bases:

>100_bases
TCCTGCTCTCCGGCAATCATCGCTTGGCCATCGGCCGCGGACAAATTATGGTTCCGGGTTCCTCGGCAGAGGGGACCGAC
ATGAAAGAGGACGCGAGGCG

Downstream 100 bases:

>100_bases
CCGTTCCGCGGTTCAGCGACCACGCGGCGAACGAGCGCACCTTCCTCGCCTGGGTGCGCACCGCGATCGCGGTGATGGCG
TTCGGCTTCCTGGTCGAACG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSLIPFDDRDGMIWWDGELRPWRDAKLHVLTHGLHYASAVFEGERSYGGNIFRLRDHTNRLIQSGKLLGFDIPFSADAID
AACNEVLKANGLTDAYIRPIAWRGSEMLAVSAQQTKIHLAIAAWPWPNLFGNDRMKGITLGMAPWRRPDPRTAPTASKAA
GLYMIGTLSKHAAEAEGFNDALMLTLDGHVAEATGANIFFAIDGELHTPTPDCFLDGITRRTVMALARARQIPVHERHIK
PEEMAKASEVFLAGTAAEVTPVRQIGELTFTPGKITETLIADYTALVHMSPEEVAKRAA

Sequences:

>Translated_299_residues
MSLIPFDDRDGMIWWDGELRPWRDAKLHVLTHGLHYASAVFEGERSYGGNIFRLRDHTNRLIQSGKLLGFDIPFSADAID
AACNEVLKANGLTDAYIRPIAWRGSEMLAVSAQQTKIHLAIAAWPWPNLFGNDRMKGITLGMAPWRRPDPRTAPTASKAA
GLYMIGTLSKHAAEAEGFNDALMLTLDGHVAEATGANIFFAIDGELHTPTPDCFLDGITRRTVMALARARQIPVHERHIK
PEEMAKASEVFLAGTAAEVTPVRQIGELTFTPGKITETLIADYTALVHMSPEEVAKRAA
>Mature_298_residues
SLIPFDDRDGMIWWDGELRPWRDAKLHVLTHGLHYASAVFEGERSYGGNIFRLRDHTNRLIQSGKLLGFDIPFSADAIDA
ACNEVLKANGLTDAYIRPIAWRGSEMLAVSAQQTKIHLAIAAWPWPNLFGNDRMKGITLGMAPWRRPDPRTAPTASKAAG
LYMIGTLSKHAAEAEGFNDALMLTLDGHVAEATGANIFFAIDGELHTPTPDCFLDGITRRTVMALARARQIPVHERHIKP
EEMAKASEVFLAGTAAEVTPVRQIGELTFTPGKITETLIADYTALVHMSPEEVAKRAA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=269, Percent_Identity=38.2899628252788, Blast_Score=187, Evalue=8e-49,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 32880; Mature: 32749

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIPFDDRDGMIWWDGELRPWRDAKLHVLTHGLHYASAVFEGERSYGGNIFRLRDHTNR
CCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCEEEEEHHHHH
LIQSGKLLGFDIPFSADAIDAACNEVLKANGLTDAYIRPIAWRGSEMLAVSAQQTKIHLA
HHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHCCEEEECCCEEEEEEEECEEEEEE
IAAWPWPNLFGNDRMKGITLGMAPWRRPDPRTAPTASKAAGLYMIGTLSKHAAEAEGFND
EEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHCCCEEEEECCHHHHHHCCCCCC
ALMLTLDGHVAEATGANIFFAIDGELHTPTPDCFLDGITRRTVMALARARQIPVHERHIK
EEEEEECCCEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
PEEMAKASEVFLAGTAAEVTPVRQIGELTFTPGKITETLIADYTALVHMSPEEVAKRAA
HHHHHHHHHEEEECCCHHHHHHHHHCCEEECCCHHHHHHHHHHHHEEEECHHHHHHHCC
>Mature Secondary Structure 
SLIPFDDRDGMIWWDGELRPWRDAKLHVLTHGLHYASAVFEGERSYGGNIFRLRDHTNR
CCCCCCCCCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCEEEEEHHHHH
LIQSGKLLGFDIPFSADAIDAACNEVLKANGLTDAYIRPIAWRGSEMLAVSAQQTKIHLA
HHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHCCEEEECCCEEEEEEEECEEEEEE
IAAWPWPNLFGNDRMKGITLGMAPWRRPDPRTAPTASKAAGLYMIGTLSKHAAEAEGFND
EEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHCCCEEEEECCHHHHHHCCCCCC
ALMLTLDGHVAEATGANIFFAIDGELHTPTPDCFLDGITRRTVMALARARQIPVHERHIK
EEEEEECCCEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
PEEMAKASEVFLAGTAAEVTPVRQIGELTFTPGKITETLIADYTALVHMSPEEVAKRAA
HHHHHHHHHEEEECCCHHHHHHHHHCCEEECCCHHHHHHHHHHHHEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]