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The map label for this gene is puuD [H]

Identifier: 148259858

GI number: 148259858

Start: 935511

End: 936251

Strand: Reverse

Name: puuD [H]

Synonym: Acry_0847

Alternate gene names: 148259858

Gene position: 936251-935511 (Counterclockwise)

Preceding gene: 148259859

Following gene: 148259857

Centisome position: 27.62

GC content: 72.87

Gene sequence:

>741_bases
ATGCCCAGCATCATCGGCATTCCCGCCTGCACCAAGTTCATCGGCAACCACCACCAGCACGCCACCCCGGCGCGCTACGG
CGAGGCGGTGATCGCGGCGAGCGACGCGCTGCCGCTGCTGATCCCGCCGGAAGGCGAGAAGATGATCGCCGTGCTCGACC
GGCTGGACGGCATCCTGTTCAACGGCAGCCCGAGCAACGTGATGCCGGCACGCTACGGCGTCGCCAATGACGAGACGCCG
GACGCGCACGACGCCGACCGCGACGCGACGACGCTGCCGCTGATCCGCGCCGCCCTTGCCCGCGGGATGCCGGTGCTCGG
CATCTGCCGCGGGTTGCAGGAGCTCAACGTCGCCCTCGGCGGCACGCTGCACCAGCAGGTCCATACCCTGCCGGGGCGGC
TCGATCACCGCGCCGGCAACGGCACGAGGGCCGAGCAGATGGCGCTCAAGCACGAGGTGCGGCTGAGCGGCGCGCTGCGG
CATATCTGCGGGGCGGAGACGATTATGGTGAATTCGCTGCACGGCCAGGCGATCGACCGGCTGGCGCCGGGGCTCGCGGT
GGAGGCGGTGGCGCCGGACGGGACGATCGAGGCGGTGCGGGTCGAGGGGGCGGCGGGCTTCGCCATTGCCGTGCAGTGGC
ACCCGGAATGGGCGGTGATGGAGGCGCCGGACCGGCAGCGGCTGTTCGCCGCCTTCGGCGAGGCGTGCCGGACCTGGCGC
GGCGGGCGCGCGGCGGCCTGA

Upstream 100 bases:

>100_bases
GCCGATGGCCTTCGCCATGTTCGCCTGCGCGGTGCTCGCCGCACTCGCCGCCGCCTTCCGACCCCAACGCCTGACCTGAC
ACGCCCCTGACGAGGACAAC

Downstream 100 bases:

>100_bases
CTTTTCGGGACGCAGCTTCTATATGGGGGGCTTACGCGGAAGGACCGAGGCCATGGATATCCAGACGATCGATTCGCAAT
ACGACCAGTTGCAGGCCCAG

Product: peptidase C26

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MPSIIGIPACTKFIGNHHQHATPARYGEAVIAASDALPLLIPPEGEKMIAVLDRLDGILFNGSPSNVMPARYGVANDETP
DAHDADRDATTLPLIRAALARGMPVLGICRGLQELNVALGGTLHQQVHTLPGRLDHRAGNGTRAEQMALKHEVRLSGALR
HICGAETIMVNSLHGQAIDRLAPGLAVEAVAPDGTIEAVRVEGAAGFAIAVQWHPEWAVMEAPDRQRLFAAFGEACRTWR
GGRAAA

Sequences:

>Translated_246_residues
MPSIIGIPACTKFIGNHHQHATPARYGEAVIAASDALPLLIPPEGEKMIAVLDRLDGILFNGSPSNVMPARYGVANDETP
DAHDADRDATTLPLIRAALARGMPVLGICRGLQELNVALGGTLHQQVHTLPGRLDHRAGNGTRAEQMALKHEVRLSGALR
HICGAETIMVNSLHGQAIDRLAPGLAVEAVAPDGTIEAVRVEGAAGFAIAVQWHPEWAVMEAPDRQRLFAAFGEACRTWR
GGRAAA
>Mature_245_residues
PSIIGIPACTKFIGNHHQHATPARYGEAVIAASDALPLLIPPEGEKMIAVLDRLDGILFNGSPSNVMPARYGVANDETPD
AHDADRDATTLPLIRAALARGMPVLGICRGLQELNVALGGTLHQQVHTLPGRLDHRAGNGTRAEQMALKHEVRLSGALRH
ICGAETIMVNSLHGQAIDRLAPGLAVEAVAPDGTIEAVRVEGAAGFAIAVQWHPEWAVMEAPDRQRLFAAFGEACRTWRG
GRAAA

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=243, Percent_Identity=41.9753086419753, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 26088; Mature: 25957

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIIGIPACTKFIGNHHQHATPARYGEAVIAASDALPLLIPPEGEKMIAVLDRLDGILF
CCCCCCCHHHHHHHCCCCCCCCCHHHCCEEEEECCCCEEEECCCCCHHHHHHHHHCCEEE
NGSPSNVMPARYGVANDETPDAHDADRDATTLPLIRAALARGMPVLGICRGLQELNVALG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
GTLHQQVHTLPGRLDHRAGNGTRAEQMALKHEVRLSGALRHICGAETIMVNSLHGQAIDR
CHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHH
LAPGLAVEAVAPDGTIEAVRVEGAAGFAIAVQWHPEWAVMEAPDRQRLFAAFGEACRTWR
HCCCCEEEEECCCCCEEEEEEECCCCEEEEEEECCCEEEECCCCHHHHHHHHHHHHHHCC
GGRAAA
CCCCCC
>Mature Secondary Structure 
PSIIGIPACTKFIGNHHQHATPARYGEAVIAASDALPLLIPPEGEKMIAVLDRLDGILF
CCCCCCHHHHHHHCCCCCCCCCHHHCCEEEEECCCCEEEECCCCCHHHHHHHHHCCEEE
NGSPSNVMPARYGVANDETPDAHDADRDATTLPLIRAALARGMPVLGICRGLQELNVALG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
GTLHQQVHTLPGRLDHRAGNGTRAEQMALKHEVRLSGALRHICGAETIMVNSLHGQAIDR
CHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHH
LAPGLAVEAVAPDGTIEAVRVEGAAGFAIAVQWHPEWAVMEAPDRQRLFAAFGEACRTWR
HCCCCEEEEECCCCCEEEEEEECCCCEEEEEEECCCEEEECCCCHHHHHHHHHHHHHHCC
GGRAAA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]