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The map label for this gene is folC [H]

Identifier: 148259855

GI number: 148259855

Start: 932765

End: 934078

Strand: Reverse

Name: folC [H]

Synonym: Acry_0844

Alternate gene names: 148259855

Gene position: 934078-932765 (Counterclockwise)

Preceding gene: 148259856

Following gene: 148259854

Centisome position: 27.56

GC content: 73.74

Gene sequence:

>1314_bases
GTGAGCGGGGCGGCGCCGGCCCCGGCACCGGCACCGAGCCGGATCGACGCGCGGATCGAGCGGCTGCACGCGCTCTATCC
GCGGCTGATCGACCTGCGGCTGGAACGGCTGGAGCGGCTGCTCGAAGCCCTGGGCCATCCGGAGCGGCGGCTGCCGCCGA
CCATTCATGTCGCCGGGACCAACGGCAAGGGCAGCGTCTGCGCCTTCCTGCGCGGCATGGTCGAGGCGGCGGGGCTGCGC
GCACATGTCTATACCTCGCCGCATCTCGTGCGGTTCAACGAGCGCATCCGGCTGGCCGGCGCGCTGGTCAGCGACGACGA
GCTGGACGCAGCGCTCGACGAGGTCGAGCGGGTGAACGACGCCGCGCCGATCACCGTGTTCGAGGCGATCACCGCCGCCG
CCTTCGTGCTGTTCGCGGCACGGTCGGCGGAGATCGCGATCATCGAGGTGGGGCTCGGCGGCACCTTCGATGCGACCAAC
GTGATCCCGCCGCCCGCCGCCGCCGCGATCACCGCGATCTCGCTCGATCACCGCGAGTTCCTCGGCGAGACGGAAACCGT
GATCGCCGCCGAGAAGGCCGGGATCATGAAGCCGGGCAGCCGGGCCGTGACCGGGTTCCAGACGCCGGACGTGCTGGCCG
TGCTGGAGTCGCGGGCGAAGGCGGCCGGGGTGCCGCTCGCCGCGCGCGGGCGGCAGTGGCGGATCATGAAGCAGGGCGAG
GGCGCGGTGTATGAGGACGAGGAGGGGCGGCTGGAGCTGCCCCGCCCCGCCCTGCCCGGCGCGCACCAGTTCGACAATGC
CGGCATCGCCATCGCCGCCCTGCGCGCCGGCGGGTTCGGGCTCGACCAGGACGCCTTCGCGCGCGGCATGGCGGCGGCGG
AATGGCCGGCACGGTTGCAGCGCCTTGGGCCGCCCTTGGCCTCGTTGCTGCCGGCGGGATCGGAACTCTGGCTCGACGGC
GCGCACAATCCGGGCGGGGCGCAGGCGCTGGCCGAGGTTCTGGCCGGCTGGGAGGGGCCGACGCATCTCGTCGTCGGGAT
GAAGCAGGCCAAGGACGTCGCGGAGTTTTTGCGCATCCTGCTGCCGCAGGCGGCATCGGTGCAGGCGGTGGTCGAGCCGG
GGCAGCATCTGGCCCTGCCCGTCGAGGCGATCATCGAGGCGTCGGGCGGGGTCGCGACGGCGGGGCCGGATGTGGAGGCC
GCGCTGCGGCGCCTCGCCGCGGACGGGACGCCGAAGCGGGTGCTGGTCTGCGGCTCGCTCTATCTCGCCGGGGTGATCCT
GGCACGGCAGGAAGCGGCGCCGGCGCAGGGCTGA

Upstream 100 bases:

>100_bases
ACGGCATGGTGGACATGGTGCTCGCGCGCGGCGAACTGGCGGCGACACTTGGGCGGCTGCTCGGCCTGCTGAAGGTGGGG
CGGCCGGACGCAGAGGCGGC

Downstream 100 bases:

>100_bases
TCCCCCTCGCCTTCCGGCCTGCCCGGCTTGCCTGAACCGCGCGATCCACCCGATACTCGCGCGGAGCAAAAAGGGAGACC
GGAATGTCCGCCACGATGAA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSGAAPAPAPAPSRIDARIERLHALYPRLIDLRLERLERLLEALGHPERRLPPTIHVAGTNGKGSVCAFLRGMVEAAGLR
AHVYTSPHLVRFNERIRLAGALVSDDELDAALDEVERVNDAAPITVFEAITAAAFVLFAARSAEIAIIEVGLGGTFDATN
VIPPPAAAAITAISLDHREFLGETETVIAAEKAGIMKPGSRAVTGFQTPDVLAVLESRAKAAGVPLAARGRQWRIMKQGE
GAVYEDEEGRLELPRPALPGAHQFDNAGIAIAALRAGGFGLDQDAFARGMAAAEWPARLQRLGPPLASLLPAGSELWLDG
AHNPGGAQALAEVLAGWEGPTHLVVGMKQAKDVAEFLRILLPQAASVQAVVEPGQHLALPVEAIIEASGGVATAGPDVEA
ALRRLAADGTPKRVLVCGSLYLAGVILARQEAAPAQG

Sequences:

>Translated_437_residues
MSGAAPAPAPAPSRIDARIERLHALYPRLIDLRLERLERLLEALGHPERRLPPTIHVAGTNGKGSVCAFLRGMVEAAGLR
AHVYTSPHLVRFNERIRLAGALVSDDELDAALDEVERVNDAAPITVFEAITAAAFVLFAARSAEIAIIEVGLGGTFDATN
VIPPPAAAAITAISLDHREFLGETETVIAAEKAGIMKPGSRAVTGFQTPDVLAVLESRAKAAGVPLAARGRQWRIMKQGE
GAVYEDEEGRLELPRPALPGAHQFDNAGIAIAALRAGGFGLDQDAFARGMAAAEWPARLQRLGPPLASLLPAGSELWLDG
AHNPGGAQALAEVLAGWEGPTHLVVGMKQAKDVAEFLRILLPQAASVQAVVEPGQHLALPVEAIIEASGGVATAGPDVEA
ALRRLAADGTPKRVLVCGSLYLAGVILARQEAAPAQG
>Mature_436_residues
SGAAPAPAPAPSRIDARIERLHALYPRLIDLRLERLERLLEALGHPERRLPPTIHVAGTNGKGSVCAFLRGMVEAAGLRA
HVYTSPHLVRFNERIRLAGALVSDDELDAALDEVERVNDAAPITVFEAITAAAFVLFAARSAEIAIIEVGLGGTFDATNV
IPPPAAAAITAISLDHREFLGETETVIAAEKAGIMKPGSRAVTGFQTPDVLAVLESRAKAAGVPLAARGRQWRIMKQGEG
AVYEDEEGRLELPRPALPGAHQFDNAGIAIAALRAGGFGLDQDAFARGMAAAEWPARLQRLGPPLASLLPAGSELWLDGA
HNPGGAQALAEVLAGWEGPTHLVVGMKQAKDVAEFLRILLPQAASVQAVVEPGQHLALPVEAIIEASGGVATAGPDVEAA
LRRLAADGTPKRVLVCGSLYLAGVILARQEAAPAQG

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=348, Percent_Identity=31.0344827586207, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI66932984, Length=348, Percent_Identity=31.0344827586207, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1788654, Length=426, Percent_Identity=31.924882629108, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17553148, Length=339, Percent_Identity=30.6784660766962, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71984923, Length=339, Percent_Identity=30.6784660766962, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17553150, Length=339, Percent_Identity=30.6784660766962, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6323760, Length=424, Percent_Identity=30.188679245283, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6324815, Length=289, Percent_Identity=27.681660899654, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6322718, Length=426, Percent_Identity=27.2300469483568, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24641571, Length=231, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24581568, Length=200, Percent_Identity=32.5, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 45672; Mature: 45541

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAAPAPAPAPSRIDARIERLHALYPRLIDLRLERLERLLEALGHPERRLPPTIHVAGT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
NGKGSVCAFLRGMVEAAGLRAHVYTSPHLVRFNERIRLAGALVSDDELDAALDEVERVND
CCCCHHHHHHHHHHHHCCCEEEEECCCEEEEECCCEEEEEEEECCHHHHHHHHHHHHHCC
AAPITVFEAITAAAFVLFAARSAEIAIIEVGLGGTFDATNVIPPPAAAAITAISLDHREF
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHEEEECHHHHH
LGETETVIAAEKAGIMKPGSRAVTGFQTPDVLAVLESRAKAAGVPLAARGRQWRIMKQGE
CCCCHHEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCEEEEEECCC
GAVYEDEEGRLELPRPALPGAHQFDNAGIAIAALRAGGFGLDQDAFARGMAAAEWPARLQ
CCEEECCCCCEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHCCHHHHHHHHHH
RLGPPLASLLPAGSELWLDGAHNPGGAQALAEVLAGWEGPTHLVVGMKQAKDVAEFLRIL
HHCCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHH
LPQAASVQAVVEPGQHLALPVEAIIEASGGVATAGPDVEAALRRLAADGTPKRVLVCGSL
CCCCCCCHHHHCCCCCEECCHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEHHH
YLAGVILARQEAAPAQG
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SGAAPAPAPAPSRIDARIERLHALYPRLIDLRLERLERLLEALGHPERRLPPTIHVAGT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
NGKGSVCAFLRGMVEAAGLRAHVYTSPHLVRFNERIRLAGALVSDDELDAALDEVERVND
CCCCHHHHHHHHHHHHCCCEEEEECCCEEEEECCCEEEEEEEECCHHHHHHHHHHHHHCC
AAPITVFEAITAAAFVLFAARSAEIAIIEVGLGGTFDATNVIPPPAAAAITAISLDHREF
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHEEEECHHHHH
LGETETVIAAEKAGIMKPGSRAVTGFQTPDVLAVLESRAKAAGVPLAARGRQWRIMKQGE
CCCCHHEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCEEEEEECCC
GAVYEDEEGRLELPRPALPGAHQFDNAGIAIAALRAGGFGLDQDAFARGMAAAEWPARLQ
CCEEECCCCCEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHCCHHHHHHHHHH
RLGPPLASLLPAGSELWLDGAHNPGGAQALAEVLAGWEGPTHLVVGMKQAKDVAEFLRIL
HHCCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHH
LPQAASVQAVVEPGQHLALPVEAIIEASGGVATAGPDVEAALRRLAADGTPKRVLVCGSL
CCCCCCCHHHHCCCCCEECCHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEHHH
YLAGVILARQEAAPAQG
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]