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The map label for this gene is tnpA [H]

Identifier: 148259717

GI number: 148259717

Start: 786201

End: 787562

Strand: Reverse

Name: tnpA [H]

Synonym: Acry_0704

Alternate gene names: 148259717

Gene position: 787562-786201 (Counterclockwise)

Preceding gene: 148259718

Following gene: 148259715

Centisome position: 23.24

GC content: 64.32

Gene sequence:

>1362_bases
ATGGCGGTTCGAGGCGGAGCTTTGGGACATTTTGGTGATGCGCGGCGCGCGGCGATCGGCAACACACTGGTTGAGCGGGT
AGTGGAAACTGGCTCTCTGGTCATACGCAAACTCGGGGGCACGCGGGCGCGGGAGATTGCGATCCATCGGTTCCTTTCGG
CCCCCTCGGTGACGTGTGGCGAGATGGTTGAGACGCTGGCGCATTGCACGGCAGTGGCCTGCAAGGGGCGTCGGATTGTG
GCGGTGCAGGACACGACAGAGATCAATTTTGCCGGCCGCGAGGAGCGTCGTCGGGGCCTTGGTCCGGCCGGAGATGGCGT
ATCGGCGGGGTTTTTCCTGCACCCGGTGATTGCGATCGACAGCGAGACGGAGGCGGTGCTCGGCCTGATCGACGCGAAAA
TATGGACCCGTTCTGATGGGTTCGATCCGACACCTGCGCGTGAGCGGGCGTTGGAGGACAAGGAGAGTGTGCGATGGTTG
GAGGGCGCTTCACACGCCGCTGACCGCCTGACCGATGCCGCCTCGGTCGTGGTTGTGGCCGACCGCGAGGGTGACATCTA
TGCGGGCTTTGCCAGGCGTCCGGCGTCGATCGAGATGATCGTCCGTGCCGCTCAGGATCGTGTTCTGGATGACGGTAGAC
GCCTATTTGCCGCCCCCGAGGCGTGGCCTGAACTGGTCCGCAGTGAGGTGCGCGTCGCGCCATCGCGCACCGGCATTGCC
GCACGGGTTGCGACCGTGGCGCTGCGTGCTGGAACGGTGACGATCTGCCGTCCGCGCCACGGCGGCGACGTGGGCGGACC
AGCGCATCTGACCCTGACCATGGTGGAGGCGCGCGAGGTCGACTGGAATGGCGAAGGCACCCCGCTCTTGTGGCGCCTGC
TCACCACGATCGAGACCATCGACGCTGACGGTGCCGCCGAAATCGTACGCCTCTACCGGCTGAGATGGAGGATTGAAGAG
GTTTTCCGGTCTTTGAAAAGTGACGGTCTCAGGCTTGAAGAAACCCAGATGCAAGATGCCGGACGGCTGTTCAAGCTGGC
CTTGATCGGCCTCGCGGCAGCGACCCGCACCGTACAACTCGTCGATGCCCGCGACGGCAGCGACCGGCCGGCAACCGACG
TCATCGATCCCGGATTACTCCCGGCCGCCGATGCCATTGCCCCCACTCTCGAAGGCAAGACACCGCGGCAAAAAAACCCC
CATCCACCGCGATCCCTCGCATGGCTCGCCTGGATCATCGCCCGCCTCGGCGGGTGGAATTGCTACTACAAGCCGCCAGG
ACCAAAGACCATGCGCGCAGGGTGGGCAACCTTCGCTTCCATGGCCACAGGCTTCATCATCGCAGCTCAATCAAATCCGT
GA

Upstream 100 bases:

>100_bases
ATCGCGAACCTCACCTATACGGCTACGGCATTCACACATTTTTCTTGCTGTTTGAGTTGGGCTCTGATTCTCGATGATCT
CTGCAGTGATGGGGAACGAG

Downstream 100 bases:

>100_bases
ATCCCGTAGCACCTATACGGGCCCTCTCAACCTGTATCAGGGGTCAGGCGCCAATAACGGGTATGGCAATATTCAGAGCT
TCCTGCAGTACGGCGGCACC

Product: transposase, IS4 family protein

Products: NA

Alternate protein names: Tnp [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MAVRGGALGHFGDARRAAIGNTLVERVVETGSLVIRKLGGTRAREIAIHRFLSAPSVTCGEMVETLAHCTAVACKGRRIV
AVQDTTEINFAGREERRRGLGPAGDGVSAGFFLHPVIAIDSETEAVLGLIDAKIWTRSDGFDPTPARERALEDKESVRWL
EGASHAADRLTDAASVVVVADREGDIYAGFARRPASIEMIVRAAQDRVLDDGRRLFAAPEAWPELVRSEVRVAPSRTGIA
ARVATVALRAGTVTICRPRHGGDVGGPAHLTLTMVEAREVDWNGEGTPLLWRLLTTIETIDADGAAEIVRLYRLRWRIEE
VFRSLKSDGLRLEETQMQDAGRLFKLALIGLAAATRTVQLVDARDGSDRPATDVIDPGLLPAADAIAPTLEGKTPRQKNP
HPPRSLAWLAWIIARLGGWNCYYKPPGPKTMRAGWATFASMATGFIIAAQSNP

Sequences:

>Translated_453_residues
MAVRGGALGHFGDARRAAIGNTLVERVVETGSLVIRKLGGTRAREIAIHRFLSAPSVTCGEMVETLAHCTAVACKGRRIV
AVQDTTEINFAGREERRRGLGPAGDGVSAGFFLHPVIAIDSETEAVLGLIDAKIWTRSDGFDPTPARERALEDKESVRWL
EGASHAADRLTDAASVVVVADREGDIYAGFARRPASIEMIVRAAQDRVLDDGRRLFAAPEAWPELVRSEVRVAPSRTGIA
ARVATVALRAGTVTICRPRHGGDVGGPAHLTLTMVEAREVDWNGEGTPLLWRLLTTIETIDADGAAEIVRLYRLRWRIEE
VFRSLKSDGLRLEETQMQDAGRLFKLALIGLAAATRTVQLVDARDGSDRPATDVIDPGLLPAADAIAPTLEGKTPRQKNP
HPPRSLAWLAWIIARLGGWNCYYKPPGPKTMRAGWATFASMATGFIIAAQSNP
>Mature_452_residues
AVRGGALGHFGDARRAAIGNTLVERVVETGSLVIRKLGGTRAREIAIHRFLSAPSVTCGEMVETLAHCTAVACKGRRIVA
VQDTTEINFAGREERRRGLGPAGDGVSAGFFLHPVIAIDSETEAVLGLIDAKIWTRSDGFDPTPARERALEDKESVRWLE
GASHAADRLTDAASVVVVADREGDIYAGFARRPASIEMIVRAAQDRVLDDGRRLFAAPEAWPELVRSEVRVAPSRTGIAA
RVATVALRAGTVTICRPRHGGDVGGPAHLTLTMVEAREVDWNGEGTPLLWRLLTTIETIDADGAAEIVRLYRLRWRIEEV
FRSLKSDGLRLEETQMQDAGRLFKLALIGLAAATRTVQLVDARDGSDRPATDVIDPGLLPAADAIAPTLEGKTPRQKNPH
PPRSLAWLAWIIARLGGWNCYYKPPGPKTMRAGWATFASMATGFIIAAQSNP

Specific function: Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After n

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559
- InterPro:   IPR003201
- InterPro:   IPR014737
- InterPro:   IPR014736
- InterPro:   IPR014735 [H]

Pfam domain/function: PF01609 Transposase_11; PF02281 Transposase_Tn5 [H]

EC number: NA

Molecular weight: Translated: 48974; Mature: 48842

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRGGALGHFGDARRAAIGNTLVERVVETGSLVIRKLGGTRAREIAIHRFLSAPSVTCG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHH
EMVETLAHCTAVACKGRRIVAVQDTTEINFAGREERRRGLGPAGDGVSAGFFLHPVIAID
HHHHHHHHHHHHHCCCCEEEEEECCCEECCCCHHHHHHCCCCCCCCCCCCCEEEEEEEEC
SETEAVLGLIDAKIWTRSDGFDPTPARERALEDKESVRWLEGASHAADRLTDAASVVVVA
CCHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEE
DREGDIYAGFARRPASIEMIVRAAQDRVLDDGRRLFAAPEAWPELVRSEVRVAPSRTGIA
ECCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCCCHH
ARVATVALRAGTVTICRPRHGGDVGGPAHLTLTMVEAREVDWNGEGTPLLWRLLTTIETI
HHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHC
DADGAAEIVRLYRLRWRIEEVFRSLKSDGLRLEETQMQDAGRLFKLALIGLAAATRTVQL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHEEEE
VDARDGSDRPATDVIDPGLLPAADAIAPTLEGKTPRQKNPHPPRSLAWLAWIIARLGGWN
EECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
CYYKPPGPKTMRAGWATFASMATGFIIAAQSNP
EEECCCCCCHHHHHHHHHHHHHCCEEEEECCCC
>Mature Secondary Structure 
AVRGGALGHFGDARRAAIGNTLVERVVETGSLVIRKLGGTRAREIAIHRFLSAPSVTCG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHH
EMVETLAHCTAVACKGRRIVAVQDTTEINFAGREERRRGLGPAGDGVSAGFFLHPVIAID
HHHHHHHHHHHHHCCCCEEEEEECCCEECCCCHHHHHHCCCCCCCCCCCCCEEEEEEEEC
SETEAVLGLIDAKIWTRSDGFDPTPARERALEDKESVRWLEGASHAADRLTDAASVVVVA
CCHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEE
DREGDIYAGFARRPASIEMIVRAAQDRVLDDGRRLFAAPEAWPELVRSEVRVAPSRTGIA
ECCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCCCHH
ARVATVALRAGTVTICRPRHGGDVGGPAHLTLTMVEAREVDWNGEGTPLLWRLLTTIETI
HHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHC
DADGAAEIVRLYRLRWRIEEVFRSLKSDGLRLEETQMQDAGRLFKLALIGLAAATRTVQL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHEEEE
VDARDGSDRPATDVIDPGLLPAADAIAPTLEGKTPRQKNPHPPRSLAWLAWIIARLGGWN
EECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
CYYKPPGPKTMRAGWATFASMATGFIIAAQSNP
EEECCCCCCHHHHHHHHHHHHHCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6260374; 6271452; 7867940; 6291786; 10884228; 12367522; 11896402 [H]